RBRC00301, B6.129S6-Rela<tm1Yuo>/2Rbrc

RBRC No. RBRC00301
Type Targeted MutationCartagena 8wks,♂
Species Mus musculus
Strain name B6.129S6-Rela<tm1Yuo>/2Rbrc
Former Common name RelA KO, RKO-2
H-2 Haplotype No Data
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]
Donor strain 129S6/SvEvTac via EK.CCE ES cell line
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Rela
Symbol name v-rel reticuloendotheliosis viral oncogene homolog A
Chromosome 19
Common name p65 NFkB, p65 NF-kappa B, avian reticuloendotheliosis viral (v-rel) oncogene homolog A
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 19
Common name neo; neomycin;
Symbol description No Data
References J Exp Med. 1997 Mar 3;185(5):953-61.
Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2994-9.

NF-kappa B RelA-deficient lymphocytes: normal development of T cells and B cells, impaired production of IgA and IgG1 and reduced proliferative responses.
NF-kappa B RelA-deficient lymphocytes: normal development of T cells and B cells, impaired production of IgA and IgG1 and reduced proliferative responses.
Research applications Cell Biology Research,
Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J Exp Med., 185, 953-961 (1997).
Any patent rights and other intellectual properties are retained by the depositor. Users should contact the depositor in the case of application for any patents with the results from these mice.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Doi, Takahiro (RIKEN, BioResource Center) Doi, Takahiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC00302, B6.129S6-Rela<tm1Yuo>/3Rbrc

RBRC No. RBRC00302
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129S6-Rela<tm1Yuo>/3Rbrc
Former Common name RelA KO, RKO-3
H-2 Haplotype No Data
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]
Donor strain 129S6/SvEvTac via EK.CCE ES cell line
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Rela
Symbol name v-rel reticuloendotheliosis viral oncogene homolog A
Chromosome 19
Common name p65 NFkB, p65 NF-kappa B, avian reticuloendotheliosis viral (v-rel) oncogene homolog A
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 19
Common name neo; neomycin;
Symbol description No Data
References J Exp Med. 1997 Mar 3;185(5):953-61.
Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2994-9.

NF-kappa B RelA-deficient lymphocytes: normal development of T cells and B cells, impaired production of IgA and IgG1 and reduced proliferative responses.
NF-kappa B RelA-deficient lymphocytes: normal development of T cells and B cells, impaired production of IgA and IgG1 and reduced proliferative responses.
Research applications Cell Biology Research,
Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J Exp Med., 185, 953-961 (1997).
Any patent rights and other intellectual properties are retained by the depositor. Users should contact the depositor in the case of application for any patents with the results from these mice.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Doi, Takahiro (RIKEN, BioResource Center) Doi, Takahiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC00305, B6.129S-Tnf<tm1Ljo> Rela<tm1Yuo>/Rbrc

RBRC No. RBRC00305
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129S-Tnf<tm1Ljo> Rela<tm1Yuo>/Rbrc
Former Common name C57BL/6-Tnf<tm1Ljo> Rela<tm1Yuo>/Rbrc, TNFκB
H-2 Haplotype No Data
ES cell line W9.5/D3 for TNF alpha, CCE [129/Sv] for relA
Donor strain RelA: 129S6/SvEvTac via EK.CCE ES cell line, THF: 129S1/Sv-p+ Tyr+ Kitl+ via W9.5 ES cell line
Background strain C57BL/6
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Produced by corssing relA deficient mice and TNF deficient mice strains. Neomycin cassettes were inserted into the nucleotides 3704-5364 of TNF gene and the downstream of the translation initiation codon of relA gene, respectively. RelA deficient mice were backcrossed to C57BL/6 for 10 generations, and TNF deficient mice were backcrossed to C57BL/6 for 5 generations. Then, the two mutant strains were crossed to create the TNFkB mice. Developed by Takahiro Doi at Aichi Cancer Research Center in 1997.
Strain description TNF and RelA double knockout mice. TNF, tumor necrosis factor is a multifunction proinflammatory cytokine. RelA, v-rel reticuloendotheliosis viral oncogene homolog A (Avian) is one of NF-kB family relating to the development of lymphocytes, suggested to be highly sensitive to TNF cytotoxicity. Mice lacking only relA gene die between days 14 and 15 of embryogenesis due to liver cell apoptosis by endogenous TNF cytotoxicity. Meanwhile, TNF/relA double-deficient mice are viable and have normal liver. This strain is useful for studies of functions of RelA in vivo.
Colony maintenance TNF: Homozygote x Homozygote; RelA: Heterozygote x Wild-type
Health Report
BRC facility Health Report in the last year and a half

Examination Date Room Rack
May 7, 2012 2-検 M
Mar 5, 2012 2-検 M
Jan 10, 2012 2-検 M
Nov 7, 2011 2-検 M
Sep 5, 2011 2-検 M
Jul 4, 2011 2-検 M
Apr 25, 2011 2-検 M
Mar 7, 2011 2-検 M

Gene Details
Promoter No Data
1 Symbol Tnf
Symbol name tumor necrosis factor
Chromosome 17
Common name tumor necrosis factor alpha, DIF, Tnfa, TNFSF2, TNFalpha, TNF-alpha, TNF alpha, Tnfsf1a, tumor necrosis factor-alpha
Symbol description No Data
2 Symbol Rela
Symbol name v-rel reticuloendotheliosis viral oncogene homolog A
Chromosome 19
Common name p65 NFkB, p65 NF-kappa B, avian reticuloendotheliosis viral (v-rel) oncogene homolog A
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 19
Common name neo; neomycin;
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 17
Common name neo; neomycin;
Symbol description No Data
References J Exp Med. 1997 Mar 3;185(5):953-61.
Proc Natl Acad Sci U S A. 1997 Jul 22;94(15):8093-8.
Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2994-9.

NF-kappa B RelA-deficient lymphocytes: normal development of T cells and B cells, impaired production of IgA and IgG1 and reduced proliferative responses.
NF-kappa B RelA-deficient lymphocytes: normal development of T cells and B cells, impaired production of IgA and IgG1 and reduced proliferative responses.
NF-kappa B RelA-deficient lymphocytes: normal development of T cells and B cells, impaired production of IgA and IgG1 and reduced proliferative responses.
NF-kappa B RelA-deficient lymphocytes: normal development of T cells and B cells, impaired production of IgA and IgG1 and reduced proliferative responses.
Research applications Apoptosis Research,
Cancer Research,
Cardiovascular Research,
Developmental Biology Research,
Diabetes and Obesity Research,
Endocrine Deficiency Research,
Hematological Research,
Immunology and Inflammation Research,
Immunology and Inflammation Research,
Internal/Organ Research,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Proc Natl Acad Sci USA., 96, 2994-2999 (1999).
The for-profit institutions should obtain written confirmation from Ludwig Institute for Cancer Research. Ludwig Institute for Cancer Research: 1275 York Avenue, New York NY 10021, USA, FAX: 1+212-717-3100
Any patent rights and other intellectual properties are retained by the depositor and Ludwig Institute for Cancer Research. Users should contact the depositor and Ludwig Institute for Cancer Research in the case of application for any patents with the results from these mice.
Additional information
1 Mouse of the Month May 2009
2 Genotyping protocol <PCR>
Depositor Doi, Takahiro (RIKEN, BioResource Center) Doi, Takahiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC00300, B6.129S6-Rela<tm1Yuo>/1Rbrc

RBRC No. RBRC00300
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129S6-Rela<tm1Yuo>/1Rbrc
Former Common name RelA KO, RKO-1
H-2 Haplotype No Data
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]
Donor strain 129S6/SvEvTac via EK.CCE ES cell line
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Rela
Symbol name v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Chromosome 19
Common name p65 NFkB, p65 NF-kappa B, avian reticuloendotheliosis viral (v-rel) oncogene homolog A
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 19
Common name neo; neomycin;
Symbol description No Data
References J Exp Med. 1997 Mar 3;185(5):953-61.
Proc Natl Acad Sci U S A. 1999 Mar 16;96(6):2994-9.

Research applications Cell Biology Research,
Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J Exp Med., 185, 953-961 (1997).
Any patent rights and other intellectual properties are retained by the depositor. Users should contact the depositor in the case of application for any patents with the results from these mice.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Doi, Takahiro (RIKEN, BioResource Center) Doi, Takahiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
A proximal kappaB site in the IL-23 p19 promoter is responsible for RelA- and c-Rel-dependent transcription. J Immunol.179(10): 6596-603 (2007).(17982049)
Mise-Omata S, Kuroda E, Niikura J, Yamashita U, Obata Y, Doi TS.
p53 regulates glucose metabolism through an IKK-NF-kappaB pathway and inhibits cell transformation. Nat Cell Biol.10(5): 611-8 (2008).(18391940)
Kawauchi K, Araki K, Tobiume K, Tanaka N.