RBRC10895, B6.129S-Gjc1<tm2.1Kni>

RBRC No. RBRC10895
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129S-Gjc1<tm2.1Kni>
Former Common name Cx45 floxed; Cx45 flox; Connexin45 floxed; Connexin45 flox
H-2 Haplotype No Data
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Gjc1
Symbol name gap junction membrane channel protein alpha 7
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 11
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data
5 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome 11
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nishii K et al., Mech Dev. 2016 May;140:41-52.
Additional information
1 No Data
Depositor Nishii, Kiyomasa (NATIONAL DEFENSE MEDICAL COLLEGE) Nishii, Kiyomasa
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC00759, B6.129S-Dad1<tm1Kni>/KniRbrc

RBRC No. RBRC00759
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129S-Dad1<tm1Kni>/KniRbrc
Former Common name Dad1 Knockout mice
H-2 Haplotype No Data
Donor strain 129S6/SvEv via CCE ES cell line
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Dad1
Symbol name defender against cell death 1
Chromosome 14
Common name No Data
Symbol description No Data
Promoter mouse RNA pol II promoter
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 14
Common name neo; neomycin;
Symbol description No Data
References Genes Cells. 1999 Apr;4(4):243-52.

Abnormalities of developmental cell death in Dad1-deficient mice.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genes Cells, 4, 243-252 (1999).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Nishii, Kiyomasa (NATIONAL DEFENSE MEDICAL COLLEGE) Nishii, Kiyomasa
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC00760, B6;129S-Gjc1<tm1Kni>/KniRbrc

RBRC No. RBRC00760
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6;129S-Gjc1<tm1Kni>/KniRbrc
Former Common name B6;129S-Gja7<tm1Kni>/KniRbrc, B6;129S-Gjc1<tm1Kni>/KniRbrc, Cx45 Knockout mice
H-2 Haplotype No Data
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]
Donor strain 129S6/SvEvTac via EK.CCE ES Cell Line
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Gjc1
Symbol name gap junction membrane channel protein alpha 7
Chromosome 11
Common name connexin45(Cx45) ; Cnx45, connexin 45, Cx45, Gja-7, Gja7
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 11
Common name No Data
Symbol description E. coli beta galactosidase
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data
5 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome 11
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
6 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References Development. 2000 Aug;127(16):3501-12.

Loss of connexin45 causes a cushion defect in early cardiogenesis.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Development, 127, 3501-3512 (2000).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Nishii, Kiyomasa (NATIONAL DEFENSE MEDICAL COLLEGE) Nishii, Kiyomasa
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months