RBRC01816, B6.129P2-Drd2<tm1(IL2RA)Koba>/KobaRbrc

RBRC No. RBRC01816
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129P2-Drd2<tm1(IL2RA)Koba>/KobaRbrc
Former Common name D2R-IL-2R
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Drd2
Symbol name dopamine receptor D2
Chromosome 9
Common name D2 receptor, D2R, Drd-2
Symbol description No Data
2 Symbol HBB2
Symbol name rabbit beta-globin poly A
Chromosome 9
Common name No Data
Symbol description No Data
3 Symbol IL2RA
Symbol name Interleukin-2 receptor α-subunit (human)
Chromosome 9
Common name No Data
Symbol description No Data
4 Symbol poly A
Symbol name SV40
Chromosome 9
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 9
Common name neo; neomycin;
Symbol description No Data
References J Neurosci. 2003 Oct 8;23(27):9078-88.

Conditional ablation of striatal neuronal types containing dopamine D2 receptor disturbs coordination of basal ganglia function.
Research applications Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neurosci. 23 (27) 9078-9088 (2003).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Lab HP (Japanese)
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC03162, B6.B6D2-Tg(Th-EGFP)6-7Koba

RBRC No. RBRC03162
Type TransgeneCartagena
Species Mus musculus
Strain name B6.B6D2-Tg(Th-EGFP)6-7Koba
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J. Two lines were generated. RBRC02095 (commonly-used), RBRC03162.
Strain description This transgenic strain expresses GFP under the control of rat tyrosine hydroxylase gene promoter.
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]
Health Report
Gene Details
Promoter rat tyrosine hydroxylase (TH) promoter
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References J Neurochem. 2002 Jul;82(2):295-304.

Dynamics of tyrosine hydroxylase promoter activity during midbrain dopaminergic neuron development.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J Neurochem. 2002 82(2):295-304.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01817, B6.129P2-Drd4<tm1(IL2RA)Tao>/KobaRbrc

RBRC No. RBRC01817
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129P2-Drd4<tm1(IL2RA)Tao>/KobaRbrc
Former Common name D4R-IL-2R
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998.
Strain description B6.129P2-Drd4<tm1(IL2RA)Tao. The human interleukin-2 receptor alpha-subunit gene and neo cassettes were introduced into exon 2 of mouse D4R gene. Specific neurons expressing D4R gene of this mice are disrupted by a recombinant immunotoxin.
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
Health Report
Gene Details
Promoter No Data
1 Symbol Drd4
Symbol name dopamine receptor 4
Chromosome 7
Common name D4R, Drd-4
Symbol description No Data
2 Symbol HBB2
Symbol name rabbit beta-globin poly A
Chromosome 7
Common name No Data
Symbol description No Data
3 Symbol IL2RA
Symbol name Interleukin-2 receptor α-subunit (human)
Chromosome 7
Common name No Data
Symbol description No Data
4 Symbol poly A
Symbol name SV40
Chromosome 7
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 7
Common name neo; neomycin;
Symbol description No Data
References J Neurosci. 2003 Dec 17;23(37):11662-72.

Dopamine D4 receptor-induced postsynaptic inhibition of GABAergic currents in mouse globus pallidus neurons.
Research applications Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Dopamine D4 receptor-induced postsynaptic inhibition of GABAergic currents in mouse globus pallidus neurons. Shin R-M, Masuda M, Miura M, Sano H, Shirasawa T, Song WJ, Kobayashi K, Aosaki T. J. Neurosci. 23 (37) 11662-11672 (2003).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC04935, C57BL/6J-Tg(Drd2-IL2RA/mVenus)4-5Koba

RBRC No. RBRC04935
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6J-Tg(Drd2-IL2RA/mVenus)4-5Koba
Former Common name B6-Drd2-IL2R/mVenus Tg#4-5
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol mVenus
Symbol name variant of enhanced yellow fluorescent protein (mVenus)
Chromosome UN
Common name Venus
Symbol description No Data
2 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome UN
Common name No Data
Symbol description No Data
Promoter rat Drd2 promoter
3 Symbol IL2RA
Symbol name Human Interleukin-2 receptor a-subunit
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Lab HP (Japanese)
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02095, B6.B6D2-Tg(Th-EGFP)21-31Koba

RBRC No. RBRC02095
Type Transgene CongenicCartagena
Species Mus musculus
Strain name B6.B6D2-Tg(Th-EGFP)21-31Koba
Former Common name B6.Cg-Tg(TH-GFP)21-31
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J. Two lines were generated. RBRC02095 (commonly-used), RBRC03162.
Strain description The TH-GFP transgenic mouse was generated to express GFP in the majority of midbrain dopamine neurons under the control of the rat TH gene promoter. Dopamine is a neurotransmitter which mediates motor control, cognition, emotion, reward, and neuroendocrine functions. Dysfunction of dopamine-producing neurons in the midbrain is involved in some neurological and neuropsychiatric diseases such as Parkinson's disease and schizophrenia. These dopamine neurons express tyrosine hydroxylase (TH), a key enzyme of catecholamine biosynthesis. This transgenic mouse is useful for visualizing dopamine neurons to study the physiology and pathogenesis of dopamine neurons. Homozygous transgenic mice are lethal.
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]
Health Report
Gene Details
Promoter rat tyrosine hydroxylase (TH) promoter
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References J Neurochem. 2002 Jul;82(2):295-304.

Dynamics of tyrosine hydroxylase promoter activity during midbrain dopaminergic neuron development.
Research applications Fluorescent Proteins/lacZ System,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neurochem., 82, 295-304 (2002).
Additional information
1 Mouse of the Month May 2008
Genetic Background
2 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
3 Construct map
Transgene sequence
4 Genotyping protocol <PCR>
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
Sensory Input Regulates Spatial and Subtype-Specific Patterns of Neuronal Turnover in the Adult Olfactory Bulb. J Neurosci.31(32): 11587-11596 (2011).(21832189)
Sawada M, Kaneko N, Inada H, Wake H, Kato Y, Yanagawa Y, Kobayashi K, Nemoto T, Nabekura J, Sawamoto K.
Structural basis for serotonergic regulation of neural circuits in the mouse olfactory bulb. J Comp Neurol. (2014).(25234191)
Suzuki Y, Kiyokage E, Sohn J, Hioki H, Toida K.
Identification of a Vav2-dependent mechanism for GDNF/Ret control of mesolimbic DAT trafficking. Nat. Neurosci. (2015).(26147533)
Zhu S, Zhao C, Wu Y, Yang Q, Shao A, Wang T, Wu J, Yin Y, Li Y, Hou J, Zhang X, Zhou G, Gu X, Wang X, Bustelo XR, Zhou J.
Differential contribution of Ih to the integration of excitatory synaptic inputs in Substantia Nigra pars compacta and Ventral Tegmental Area dopaminergic neurons. Eur. J. Neurosci. (2015).(26354486)
Masi A,, Narducci R, Resta F, Carbone C, Kobayashi K, Mannaioni G.
Spatial distribution of synapses on tyrosine hydroxylase expressing juxtaglomerular cells in the mouse olfactory glomerulus. J. Comp. Neurol. (2016).(27864931)
Kiyokage E, Kobayashi K, Toida K.

RBRC04936, C57BL/6J-Tg(Npy-IL2RA/mVenus)984-2Koba

RBRC No. RBRC04936
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6J-Tg(Npy-IL2RA/mVenus)984-2Koba
Former Common name B6-NPY-IL2R/mVenus Tg#984-2
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol mVenus
Symbol name variant of enhanced yellow fluorescent protein (mVenus)
Chromosome UN
Common name Venus
Symbol description No Data
Promoter mouse mouse Neuropeptide Y promoter
2 Symbol IL2RA
Symbol name Human Interleukin-2 receptor a-subunit
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Lab HP (Japanese)
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02140, B6.Cg-Tg(DBH-tauGFP)17

RBRC No. RBRC02140
Type Transgene CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Tg(DBH-tauGFP)17
Former Common name B6.Cg-(DBH-tauGFP)17
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The Tg mice were backcrossed to C57BL/6J over 20 times. Line 17 (RBRC02140), line 36 (RBRC02141).
Strain description These transgenic mice express a fusion gene of bovine tau protein and GFP under the control of the human DBH promoter in typical noradrenergic and adrenergic cell groups. Line 17 and 36 were generated. The expression level of Tg in each line was following order: 17 > 36 (developer's information).
Health Report No Data
Gene Details
Promoter human dopamine beta hydroxylase (DBH) promoter
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol tau
Symbol name bovine tau protein cDNA
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neurogastroenterol. Motil. 22(3):331-e86. PMID: 19775251
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05424, B6;129-Drd1<tm2(IL2RA/mVenus)Koba

RBRC No. RBRC05424
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129-Drd1<tm2(IL2RA/mVenus)Koba
Former Common name B6-Drd1/IL2RAmVenus KI
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Kazuto Kobayashi, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine in 2010. A human IL2RA and mVenus fusion gene was transferred into 129 derived ES cells. The mutant mice were crossed to C57BL/6J for several times.
Strain description The human IL2RA and mVenus fusion gene was inserted into the start codon of Drd1 gene.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome 9
Common name No Data
Symbol description No Data
2 Symbol Drd1
Symbol name dopamine receptor D1
Chromosome 9
Common name No Data
Symbol description No Data
3 Symbol IL2RA
Symbol name Human Interleukin-2 receptor alpha-subunit
Chromosome 9
Common name No Data
Symbol description No Data
4 Symbol mVenus
Symbol name variant of enhanced yellow fluorescent protein (mVenus)
Chromosome 9
Common name No Data
Symbol description No Data
References

Research applications Fluorescent Proteins/lacZ System,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Lab HP (Japanese)
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02141, B6.Cg-Tg(DBH-tauGFP)36

RBRC No. RBRC02141
Type Transgene CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Tg(DBH-tauGFP)36
Former Common name B6.Cg-(DBH-tauGFP)36
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The Tg mice were backcrossed to C57BL/6J over 20 times. Line 17 (RBRC02140), line 36 (RBRC02141).
Strain description These transgenic mice express a fusion gene of bovine tau protein and GFP under the control of the human DBH promoter in typical noradrenergic and adrenergic cell groups. Line 17 and 36 were generated. The expression level of Tg in each line was following order: 17 > 36 (developer's information).
Health Report No Data
Gene Details
Promoter human dopamine beta hydroxylase (DBH) promoter
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol tau
Symbol name bovine tau protein cDNA
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neurogastroenterol. Motil. 22(3):331-e86. PMID: 19775251
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09464, B6;Tg(ChAT-IL-2Ra/mVenus)997-6

RBRC No. RBRC09464
Type TransgeneCartagena
Species Mus musculus
Strain name B6;Tg(ChAT-IL-2Ra/mVenus)997-6
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol mVenus
Symbol name variant of enhanced yellow fluorescent protein (mVenus)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter Rat Choline acetyltransferase (ChAT) promoter
3 Symbol IL2RA
Symbol name Homo sapiens interleukin 2 receptor, alpha (IL2RA)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Sci. Rep., 5:13158 (2015).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months