RBRC01298, CAT-Rho-K DN/5-18

RBRC No. RBRC01298
Type TransgeneCartagena
Species Mus musculus
Strain name CAT-Rho-K DN/5-18
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6J
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene containing CMV promoter and bovine Rho-K DN cDNA separated by a floxed chloramphenicol acetyltransferase (CAT) cassette was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J.
Strain description These transgenic mice express a dominant-negative form for RhoA for Rho-kinase (Rho-K DN) by using the Cre-loxP system to suppress the activity of these signaling molecules in developing tissues. The signaling through Rho GTPase is important in the regulation of cellular processes such as cell morphology, cell adhesion, cell motility, and apoptosis. Copy number: line 3-1 (RBRC01294)_10 copies, line 5-18 (RBRC01298)_1 copy.
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]  Tg mice are viable and fertile.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Rock2
Symbol name Rho-associated coiled-coil containing protein kinase 2 (bovine)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CMV promoter, rabbit beta-globin intron, rabbit beta-globin poly A, SV40 early gene poly A
3 Symbol cat
Symbol name E. coli Chloramphenicol acetyltransferase (CAT)
Chromosome UN
Common name No Data
Symbol description No Data
References J Neurosci 2004 Apr 7;24(14):3480-8.

Survival of developing motor neurons mediated by Rho GTPase signaling pathway through Rho-kinase.
Research applications Cre/loxP system,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neursci., 24, 3480-3488 (2004).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Lab HP (Japanese)
2 Mouse of the Month Jun 2005
3 Genotyping protocol <PCR>
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC03070, B6;129-Sst<tm1Koba>

RBRC No. RBRC03070
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129-Sst<tm1Koba>
Former Common name B6.Cg-Tg(SST-IL-2Rα/YFP)
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Sst
Symbol name somatostatin
Chromosome 16
Common name No Data
Symbol description No Data
2 Symbol IL2RA
Symbol name Interleukin-2 receptor α-subunit (human)
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol YFP
Symbol name Yellow Fluorescent Protein (Jellyfish)
Chromosome No Data
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT shall contact the DEPOSITOR (Dr. Kazuto Kobayasi, kazuto@fmu.ac.jp) kazuto@fmu.ac.jp about the use of the BIOLOGICAL RESOURCE in advance.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01492, B6.Cg-Tg(DBH-cre)9-9Koba/KobaRbrc

RBRC No. RBRC01492
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(DBH-cre)9-9Koba/KobaRbrc
Former Common name DBH-Cre/9-9
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J.
Strain description These transgenic mice express Cre recombinase gene under the control of the human dopamin beta hydroxylase (DBH) gene promoter. This strain can be used to make an inducible knock-out by crossing with the other genetically-engineered mice that have a loxP site-flanked sequence of interest. The recombination occurs in typical noradrenergic and adrenergic cell groups as well as in transient catecholaminergic cell types. Line 6-8 (RBRC01297), Line 9-9 (RBRC01492).
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]
Health Report
Gene Details
Promoter human dopamine beta-hydroxylase (DBH) promoter, rabbit beta-globin intron, rabbit beta-globin poly A, SV40 early gene poly A
1 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References J Neurosci Res. 2004 Oct 1;78(1):7-15.

Fate of transient catecholaminergic cell types revealed by site-specific recombination in transgenic mice.
Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neurosci. Res., 78, 7-15 (2004).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Lab HP (Japanese)
2 Mouse of the Month June 2006
3 Genotyping protocol <PCR>
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC03071, B6;129-Pvalb<tm1Koba>

RBRC No. RBRC03071
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129-Pvalb<tm1Koba>
Former Common name B6.Cg-Tg(PVA-IL-2Rα/YFP)
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Pvalb
Symbol name parvalbmin
Chromosome 15
Common name No Data
Symbol description No Data
2 Symbol IL2RA
Symbol name Interleukin-2 receptor α-subunit (human)
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol YFP
Symbol name Yellow Fluorescent Protein (Jellyfish)
Chromosome No Data
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT shall contact the DEPOSITOR (Dr. Kazuto Kobayasi, kazuto@fmu.ac.jp) kazuto@fmu.ac.jp about the use of the BIOLOGICAL RESOURCE in advance.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01527, NP-IL2R/2-6

RBRC No. RBRC01527
Type TransgeneCartagena
Species Mus musculus
Strain name NP-IL2R/2-6
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse neropsin (NP) promoter, rabbit beta-globin intron,
rabbit beta-globin poly A, SV40 early gene poly A
2 Symbol IL2RA
Symbol name Interleukin-2 receptor α-subunit (human)
Chromosome UN
Common name No Data
Symbol description No Data
References J Neurosci 2005 Aug 24;25(34):7743-53.

Subthalamic neurons coordinate basal ganglia function through differential neural pathways.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neurosci., 25 (34) 7743-7753 (2005).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC03110, C57BL/6J-Tg(mDrd1-YFP)264-7Koba

RBRC No. RBRC03110
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6J-Tg(mDrd1-YFP)264-7Koba
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Drd1 promoter
2 Symbol YFP
Symbol name Yellow Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neuron, 89 (3) 550-565 (2016).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT shall contact the DEPOSITOR (Dr. Kazuto Kobayasi, kazuto@fmu.ac.jp) about the use of the BIOLOGICAL RESOURCE in advance.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01528, NP-IL2R/3-10

RBRC No. RBRC01528
Type TransgeneCartagena
Species Mus musculus
Strain name NP-IL2R/3-10
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse neropsin (NP) promoter, rabbit beta-globin intron,
rabbit beta-globin poly A, SV40 early gene poly A
2 Symbol IL2RA
Symbol name Interleukin-2 receptor α-subunit (human)
Chromosome UN
Common name No Data
Symbol description No Data
References J Neurosci 2005 Aug 24;25(34):7743-53.

Subthalamic neurons coordinate basal ganglia function through differential neural pathways.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neurosci., 25 (34) 7743-7753 (2005).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC03111, C57BL/6J-Tg(mDrd1-YFP)680-1Koba

RBRC No. RBRC03111
Type TransgeneCartagena wks
Species Mus musculus
Strain name C57BL/6J-Tg(mDrd1-YFP)680-1Koba
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Kazuto Kobayashi, Fukushima Medical University School of Medicine in 2009.
Strain description This transgenic mice express a yellow fluorescent protein under the control of the mouse Drd1a (dopamine receptor D1A gene promoter.
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]
Health Report
Gene Details
Promoter mouse Drd1 promoter
1 Symbol YFP
Symbol name Yellow Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neuron, 89 (3) 550-565 (2016).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT shall contact the DEPOSITOR (Dr. Kazuto Kobayasi, kazuto@fmu.ac.jp) about the use of the BIOLOGICAL RESOURCE in advance.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Phosphoproteomics of the Dopamine Pathway Enables Discovery of Rap1 Activation as a Reward Signal In Vivo. Neuron89(3): 550-65. (2016).(26804993)
Nagai T, Nakamuta S, Kuroda K, Nakauchi S, Nishioka T, Takano T, Zhang X, Tsuboi D, Funahashi Y, Nakano T, Yoshimoto J, Kobayashi K, Uchigashima M, Watanabe M, Miura M, Nishi A, Kobayashi K, Yamada K, Amano M, Kaibuchi K.
Dopamine D1 Receptor Immunoreactivity on Fine Processes of GFAP-Positive Astrocytes in the Substantia Nigra Pars Reticulata of Adult Mouse. Front Neuroanat11: 3 (2017).(28203148)
Nagatomo K, Suga S, Saitoh M, Kogawa M, Kobayashi K, Yamamoto Y, Yamada K.
Cell type-specific activation of mitogen-activated protein kinase in D1 receptor-expressing neurons of the nucleus accumbens potentiates stimulus-reward learning in mice. Sci. Rep.8(1): 14413. (2018).(30258218)
Bin Saifullah MA, Nagai T, Kuroda K, Wulaer B, Nabeshima T, Kaibuchi K, Yamada K.

RBRC01816, B6.129P2-Drd2<tm1(IL2RA)Koba>/KobaRbrc

RBRC No. RBRC01816
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129P2-Drd2<tm1(IL2RA)Koba>/KobaRbrc
Former Common name D2R-IL-2R
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Drd2
Symbol name dopamine receptor D2
Chromosome 9
Common name D2 receptor, D2R, Drd-2
Symbol description No Data
2 Symbol HBB2
Symbol name rabbit beta-globin poly A
Chromosome 9
Common name No Data
Symbol description No Data
3 Symbol IL2RA
Symbol name Interleukin-2 receptor α-subunit (human)
Chromosome 9
Common name No Data
Symbol description No Data
4 Symbol poly A
Symbol name SV40
Chromosome 9
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 9
Common name neo; neomycin;
Symbol description No Data
References J Neurosci. 2003 Oct 8;23(27):9078-88.

Conditional ablation of striatal neuronal types containing dopamine D2 receptor disturbs coordination of basal ganglia function.
Research applications Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neurosci. 23 (27) 9078-9088 (2003).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Lab HP (Japanese)
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC03162, B6.B6D2-Tg(Th-EGFP)6-7Koba

RBRC No. RBRC03162
Type TransgeneCartagena
Species Mus musculus
Strain name B6.B6D2-Tg(Th-EGFP)6-7Koba
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Kazuto Kobayashi, Fukushima Medical University School of Medicine in 1998. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J. Two lines were generated. RBRC02095 (commonly-used), RBRC03162.
Strain description This transgenic strain expresses GFP under the control of rat tyrosine hydroxylase gene promoter.
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]
Health Report
Gene Details
Promoter rat tyrosine hydroxylase (TH) promoter
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References J Neurochem. 2002 Jul;82(2):295-304.

Dynamics of tyrosine hydroxylase promoter activity during midbrain dopaminergic neuron development.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J Neurochem. 2002 82(2):295-304.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kobayashi, Kazuto (Fukushima Medical University) Kobayashi, Kazuto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months