RBRC00897, B6.Cg-Aicda<tm1Hon> (N10)/HonRbrc

RBRC No. RBRC00897
Type Targeted Mutation CongenicCartagena wks
Species Mus musculus
Strain name B6.Cg-Aicda<tm1Hon> (N10)/HonRbrc
Former Common name AID-KO C57BL/6 N10, B6-AID KO#N10, B6.Cg-Aicda<tm1Hon> (N10)/HonRbrc
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Donor strain (C57BL/6 x CBA)F1 via TT2 ES cell line
Background strain No Data
Appearance
1 Appearance Black
Genotype a/a B/B C/C
Strain development Developed by Tasuku Honjo, Graduate School of Medicine, Kyoto University. The construct was electoroporated into TT2 ES cells derived from (C57BL/6 x CBA)F1. The mutant mice were backcrossed to C57BL/6N.
Strain description AID (Aicda, activation-induced cytidine deaminase) knockout mice. Exon 2 and part of exon 3 were replaced with a neomycin resistance cassette. Aid, Activation-Induced cytidine Deaminase is a molecule which specifically expressed in B lymphocytes after antigen stimulation. AID deficiency caused a complete defect in class switching and showed a hyper-IgM phenotype with enlarged germinal centers containing strongly activated B cells before or after immunization. Accumulation of IgM positive B cell is remarkable in the gut lamina propria. Furthermore, it is suggested that a mutation of the Aid gene was a cause of the hereditary immune deficiency disorder, type-2 hyper-IgM syndrome (HIGM2) in human. As well as class switch recombination, it was also indicated that somatic hypermutation of an immunoglobulin variable region gene and gene conversion do not occur at all in the Aid deficiency mice. Aid gene is a molecule serving as a central role in antibody gene variety acquisition mechanism of B cell.
Colony maintenance Homozygote x Homozygote. Homozygous mutant mice are viable and fertile.
Health Report
Gene Details
Promoter No Data
1 Symbol Aicda
Symbol name Activation-induced cytidine deaminase
Chromosome 6
Common name AID
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name neo; neomycin;
Symbol description No Data
References Cell. 2000 Sep 1;102(5):553-63.

Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme.
Research applications Cre/loxP system,
Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Cell 102, 553-563 (2000).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
Additional information
1 Honjo Lab HP
2 Mouse of the Month Dec 2005
Genetic Background
3 Genotyping protocol <PCR>
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University) Honjo, Tasuku
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
IgG and IgE Collaboratively Accelerate Expulsion of Strongyloides venezuelensis in a Primary Infection. Infect Immun.81(7): 2518-27 (2013).(23630966)
Matsumoto M, Sasaki Y, Yasuda K, Takai T, Muramatsu M, Yoshimoto T, Nakanishi K.
Class switch recombination and somatic hypermutation of virus-neutralizing antibodies are not essential for control of friend retrovirus infection. J. Virol.89(2): 1468-73 (2014).(25378499)
Kato M, Tsuji-Kawahara S, Kawasaki Y, Kinoshita S, Chikaishi T, Takamura S, Fujisawa M, Kawada A, Miyazawa M.

RBRC04892, B6(Cg)-Aicda<tm2.1Hon>

RBRC No. RBRC04892
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6(Cg)-Aicda<tm2.1Hon>
Former Common name B6-AID KI/G23S
H-2 Haplotype No Data
ES cell line Bruce 4 [B6.Cg-Thy1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Min Wei and Tasuku Honjo, Gradutate School of Medicine, Kyoto University in 2007.
Strain description No Data
Colony maintenance Heterozygote x Wild-type [C57BL/6J(Crlj)]
Health Report
Gene Details
Promoter No Data
1 Symbol Aicda
Symbol name activation-induced cytidine deaminase
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Immunol., 12, 264-270 (2011).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
Additional information
1 Honjo Lab HP
2 Genotyping protocol <PCR>
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University) Honjo, Tasuku
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC00898, B6.Cg-Aicda<tm1Hon>/HonRbrc

RBRC No. RBRC00898
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Aicda<tm1Hon>/HonRbrc
Former Common name AID-KO (C57BL/6 X CBA) C57BL/6; AID mixed background
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Donor strain (C57BL/6 x CBA)F1 via TT2 ES cell line
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Aicda
Symbol name Activation-induced cytidine deaminase
Chromosome 6
Common name AID
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name neo; neomycin;
Symbol description No Data
References Cell. 2000 Sep 1;102(5):553-63.

Class switch recombination and hypermutation require activation-induced cytidine deaminase (AID), a potential RNA editing enzyme.
Research applications Cre/loxP system,
Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Cell 102, 553-563 (2000).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
Additional information
1 Honjo Lab HP
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University) Honjo, Tasuku
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC05550, B6.Cg-Ighm<tm1Cgn> Tg(Aicda-cre)9Mbu Gt(ROSA)26Sor<tm1Sho>

RBRC No. RBRC05550
Type Targeted Mutation and TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Ighm<tm1Cgn> Tg(Aicda-cre)9Mbu Gt(ROSA)26Sor<tm1Sho>
Former Common name muMT/Aicda-cre/R26R, B6-Ighm KO/AicdaCre/ROSA26-lacZ
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development muMT : Klaus Rajewsky, Insitute for Genetics, University of Cologne. R26R : Philippe Soriano, Mount Sinal School of Medicine. Aicda-cre : Meinrad Busslinger. Research Institute of Molecular Pathology.
Strain description This strain has Ighm knockout (Ighm<tm1Cgn>), AID-Tg (Tg(Aicda-cre)9Mbu) and ROSA26 lacZ reporter (Gt(ROSA)26Sor<tm1Sho>) alleles. Immunodeficient.
Colony maintenance Ighm : Homozygote x Homozygote ; Tg : Carrier x Carrier ; ROSA26 : Homozygote x Homozygote
Health Report
BRC facility Health Report in the last year and a half

Examination Date Room Rack
Nov 9, 2015 2-検 G
Sep 7, 2015 2-検 G
Jul 6, 2015 2-検 G
May 11, 2015 2-検 G
Mar 9, 2015 2-検 G
Jan 13, 2015 2-検 G
Nov 10, 2014 2-検 G
Sep 8, 2014 2-検 G

Gene Details
Promoter No Data
1 Symbol Ighm
Symbol name immunoglobulin heavy constant mu
Chromosome 12
Common name No Data
Symbol description No Data
2 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome No Data
Common name neo; neomycin;
Symbol description No Data
4 Symbol CD2 (human)
Symbol name CD2 (human)
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter mouse Aicda promoter
5 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References Immunity. 2008 Jun;28(6):751-62.


Instructive role of the transcription factor E2A in early B lymphopoiesis and germinal center B cell development.
Instructive role of the transcription factor E2A in early B lymphopoiesis and germinal center B cell development.
Instructive role of the transcription factor E2A in early B lymphopoiesis and germinal center B cell development.
Instructive role of the transcription factor E2A in early B lymphopoiesis and germinal center B cell development.
Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. PLoS One. 2011 6(12):e29141.
2) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
3) The BIOLOGICAL RESOURCE contains Tg(Aicda-re)9Mbu and Gt(ROSA)26Sortm1Sho. Before executing the MTA between RIKEN BRC and the RECIPIENT, the RECIPIENT shall contact Dr. Meinrad Busslinger, Research Institute of Molecular Pathology and shall execute the material transfer agreement with Research Institute of Molecular Pathology to use Tg(Aicda-cre)9Mbu. The RECIPIENT shall obtain the prior written consent of Dr. Philippe Soriano, Mount Sinai School of Medicine to use Gt(ROSA)26Sortm1Sho.
Additional information
1 Honjo Lab HP
2 Genotyping protocol <PCR>
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University) Honjo, Tasuku
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC00903, C.129S2-Pdcd1<tm1Hon>/HonRbrc (N10)

RBRC No. RBRC00903
Type Targeted Mutation CongenicCartagena wks
Species Mus musculus
Strain name C.129S2-Pdcd1<tm1Hon>/HonRbrc (N10)
Former Common name PD-1-KO-N10 (BALB/c)
H-2 Haplotype No Data
ES cell line D3 [129S2/SvPas]
Donor strain 129S2/SvPas via D3 ES cell line
Background strain BALB/c
Appearance
1 Appearance albino
Genotype A/A b/b c/c
Strain development Developed by Dr. Tasuku Honjo, Kyoto University
Strain description The PD1 (programmed cell death 1) gene is an immunoinhibitory receptor that belongs to the CD28 family, and plays a role in the negative control of proliferation, differentiation and class switching of B cells. Homozygous mutant mice exhibit abnormalities in leukopoiesis and immune system which vary considerably depending on the genetic background. The homozygous mutant mice with BALB/c background exhibit autoimmune dilated cardiomyopathy. The mutant mice of the BALB/c-PD1 (N10 line) show severe dilated cardiomyopathy and start to die as early as 5 weeks of age. Meanwhile, the BALB/c-PD1 (N12) line mutant mice exhibit less severe dilated cardiomyopathy and survive longer than N10 line.
Colony maintenance Sibling Mating (Homozygote x Homozygote)
Homozygous mutant mice are fertile and viable.
Health Report
Gene Details
Promoter No Data
1 Symbol Pdcd1
Symbol name programmed cell death 1
Chromosome 1
Common name Ly101, PD-1, Pdc1
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 1
Common name neo; neomycin;
Symbol description No Data
References Immunity. 1999 Aug;11(2):141-51.
Int Immunol. 1998 Oct;10(10):1563-72.
Science. 2001 Jan 12;291(5502):319-22.

Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Science 291, 319-322 (2001).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
Additional information
1 Honjo Lab HP
2 Mouse of the Month Mar 2005
3 Genotyping protocol <PCR>
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University) Honjo, Tasuku
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC05551, B6.Cg-Tg(Aicda-cre)9Mbu Gt(ROSA)26Sor<tm1Hjf>

RBRC No. RBRC05551
Type Targeted Mutation and TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(Aicda-cre)9Mbu Gt(ROSA)26Sor<tm1Hjf>
Former Common name Aicda-cre/rosa-tdRFP, B6-AicdaCre/ROSA26-tdRFP
H-2 Haplotype No Data
ES cell line AK7 [129S4/SvJaeSor]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hitoshi Nagaoka, Graduate School of Medicine, Kyoto University. Rosa-tdRFP : Hans Jorg Fehling, Institute of Immunology, University Clinics Ulm. Aicda-cre : Meinrad Busslinger. Research Institute of Molecular Pathology.
Strain description This strain has AID-Tg (Tg(Aicda-cre)9Mbu) and ROSA26 tdRFP reporter (Gt(ROSA)26Sor<tm1Hjf>) alleles.
Colony maintenance Tg : Carrier x Carrier ; ROSA26 : Homozygote x Homozygote
Health Report
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol RFP
Symbol name Red Fluorescent Protein (Discosoma sp.)
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol SA
Symbol name Splice acceptor (adenovirus)
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol lox2272
Symbol name mutant lox sequence
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
7 Symbol No Data
Symbol name SV40 nuclear localization sequence
Chromosome UN
Common name No Data
Symbol description No Data
8 Symbol CD2 (human)
Symbol name CD2 (human)
Chromosome UN
Common name No Data
Symbol description No Data
9 Symbol Ires
Symbol name internal ribosomal entry site (EMCV)
Chromosome UN
Common name No Data
Symbol description No Data
10 Symbol poly A
Symbol name SV40 polyadenylation site
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Aicda promoter
11 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References Eur J Immunol. 2007 Jan;37(1):43-53.
Immunity. 2008 Jun;28(6):751-62.

Faithful activation of an extra-bright red fluorescent protein in "knock-in" Cre-reporter mice ideally suited for lineage tracing studies.
Faithful activation of an extra-bright red fluorescent protein in "knock-in" Cre-reporter mice ideally suited for lineage tracing studies.
Faithful activation of an extra-bright red fluorescent protein in "knock-in" Cre-reporter mice ideally suited for lineage tracing studies.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. PLoS One. 2011 6(12):e29141.
2) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
3) The BIOLOGICAL RESOURCE contains Tg(Aicda-re)9Mbu and Gt(ROSA)26Sortm1Hjf. Before executing the MTA between RIKEN BRC and the RECIPIENT, the RECIPIENT shall contact Dr. Meinrad Busslinger, Research Institute of Molecular Pathology and shall execute the material transfer agreement with Research Institute of Molecular Pathology to use Tg(Aicda-cre)9Mbu. The RECIPIENT shall obtain the prior written consent of Dr. Hans Joerg Fehling, Institute of Immunology, University Clinics Ulm to use Gt(ROSA)26Sortm1Hjf.
Additional information
1 Honjo Lab HP
2 Genotyping protocol <PCR>
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University) Honjo, Tasuku
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC00904, C.129S2-Pdcd1<tm1Hon>/HonRbrc (N12)

RBRC No. RBRC00904
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name C.129S2-Pdcd1<tm1Hon>/HonRbrc (N12)
Former Common name PD-1-KO-N12 (BALB/c)
H-2 Haplotype No Data
ES cell line D3 [129S2/SvPas]
Donor strain 129S2/SvPas via D3 ES cell line
Background strain BALB/cCrSlc
Appearance
1 Appearance albino
Genotype A/A b/b c/c
Strain development Developed by Dr. Tasuku Honjo, Kyoto University.
Strain description The PD1 (programmed cell death 1) gene is an immunoinhibitory receptor that belongs to the CD28 family, and plays a role in the negative control of proliferation, differentiation and class switching of B cells. Homozygous mutant mice exhibit abnormalities in leukopoiesis and the immune system which vary considerably depending on the genetic background. The homozygous mutant mice with BALB/c background exhibit autoimmune dilated cardiomyopathy. The mutant mice of the BALB/c-PD1 (N10 line) show severe dilated cardiomyopathy and start to die as early as 5 weeks of age. Meanwhile, the BALB/c-PD1 (N12) line mutant mice exhibit less severe dilated cardiomyopathy and survive longer than N10 line.
Colony maintenance Sibling Mating (Homozygote x Homozygote)
Homozygous mutant mice are fertile and viable.
Health Report
Gene Details
Promoter No Data
1 Symbol Pdcd1
Symbol name programmed cell death 1
Chromosome 1
Common name Ly101, PD-1, Pdc1
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 1
Common name neo; neomycin;
Symbol description No Data
References Immunity. 1999 Aug;11(2):141-51.
Int Immunol. 1998 Oct;10(10):1563-72.
Science. 2001 Jan 12;291(5502):319-22.

Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
Development of lupus-like autoimmune diseases by disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor.
Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Science 291, 319-322 (2001).
1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Science 291, 319-322 (2001).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
Additional information
1 Honjo Lab HP
2 Mouse of the Month Mar 2005
3 Genotyping protocol <PCR>
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University) Honjo, Tasuku
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
Chemotherapy rescues tumor-driven aberrant CD4+ T-cell differentiation and restores an activated polyfunctional helper phenotype. Blood.115(12): 2397-406 (2010).(20118405)
Ding ZC, Blazar BR, Mellor AL, Munn DH, Zhou G.
Polyfunctional CD4+ T cells are essential for eradicating advanced B-cell lymphoma after chemotherapy. Blood. (2012).(22859605)
Ding ZC, Huang L, Blazar BR, Yagita H, Mellor AL, Munn DH, Zhou G.
Immunosuppressive myeloid cells induced by chemotherapy attenuate antitumor CD4+ T cell responses through the PD-1/PD-L1 axis. Cancer Res. (2014).(24780756)
Ding ZC, Lu X, Yu M, Lemos H, Huang L, Chandler P, Liu K, Walters M, Krasinski A, Mack M, Blazar BR, Mellor AL, Munn DH, Zhou G.

RBRC05938, C57BL/6-Tg(Aicda*dME-cre,-CD2)1Hon

RBRC No. RBRC05938
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(Aicda*dME-cre,-CD2)1Hon
Former Common name B6.Tg(Aicda-cre)dME, dMe-ac-cre
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Tasuku Honjo, Graduate School of Medicine. Kyoto University in 2010.
Strain description dME-ac-cre transgenice mice, in which the region 2 c-Myb- and E2F-binding sites-the silencing elements- in the Aicda enhancer were deleted.
Colony maintenance Carrier x Noncarrier [C57BL/6NCrSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 polyA signal
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol CD2
Symbol name human
CD2 cDNA
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome UN
Common name No Data
Symbol description No Data
4 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome UN
Common name No Data
Symbol description No Data
5 Symbol Ires
Symbol name internal ribosomal entry site (EMCV)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Aicda promoter
6 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested. PLoS One, 8, e61433 (2013).
Additional information
1 Honjo Lab HP
2 Genotyping protocol <PCR>
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University) Honjo, Tasuku
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01071, Floxed RBP-J

RBRC No. RBRC01071
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name Floxed RBP-J
Former Common name No Data
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Donor strain Not Specified
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Tasuku Honjo, Graduate School of Medicine, Kyoto University. The construct was electoroporated into E14 ES cells derived from 129P2/OlaHsd. The mutant mice were crossed to C57BL/6.
Strain description Rbpj floxed mice. Rbpj, recombination signal binding protein for immunoglobulin kappa J region is a transcriptional factor of a Notch/RBP-J signaling pathway play a role in fate determination in various lineages. Exon 6 and 7 of Rbpj gene was flanked by loxP sites, and a neomycin resistance gene was inserted between exon 7 and the loxP site. Conditional RBP-J deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenance Homozygote x Homozygote. Homozygous mice are viable and fertile.
Health Report
Gene Details
Promoter No Data
1 Symbol Rbpj
Symbol name recombining binding protein suppressor of hairless (Drosophila)
Chromosome 5
Common name RBPf, RBP-JC, RBP-Jf, Rbpjflox, Rbp-Jflox, RBP-JkappaFl, RBP-Jkappaflox, RBPJkappaloxP, RBP-Jlox, RbpjloxP, Rbpsuhtm3Kyo
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 5
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 5
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 5
Common name neo; neomycin;
Symbol description No Data
References Int Immunol. 2002 Jun;14(6):637-45.

Notch signaling controls liver development by regulating biliary differentiation.
Notch signaling controls liver development by regulating biliary differentiation.
Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Int, Immunol., 14, 637-645 (2002).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
Additional information
1 Honjo Lab HP
2 Genetic Background
3 Genotyping protocol <PCR>
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University) Honjo, Tasuku
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
Notch signaling controls liver development by regulating biliary differentiation. Development.136(10): 1727-39 (2009).(19369401)
Zong Y, Panikkar A, Xu J, Antoniou A, Raynaud P, Lemaigre F, Stanger BZ.
RBP-J promotes neuronal differentiation and inhibits oligodendroglial development in adult neurogenesis. Dev Biol.332(2): 339-50 (2009).(19501584)
Fujimoto M, Takagi Y, Muraki K, Nozaki K, Yamamoto N, Tsuji M, Hashimoto N, Honjo T, Tanigaki K.
Regulation of myeloid leukaemia by the cell-fate determinant Musashi. Nature.466(7307): 765-8 (2010).(20639863)
Ito T, Kwon HY, Zimdahl B, Congdon KL, Blum J, Lento WE, Zhao C, Lagoo A, Gerrard G, Foroni L, Goldman J, Goh H, Kim SH, Kim DW, Chuah C, Oehler VG, Radich JP, Jordan CT, Reya T.
Notch pathway regulation of neural crest cell development in vivo. Dev Dyn.241(2): 376-89 (2012).(22275227)
Mead TJ, Yutzey KE.
Epithelial Cell-Intrinsic Notch Signaling Plays an Essential Role in the Maintenance of Gut Immune Homeostasis. J Immunol. (2012).(22279105)
Obata Y, Takahashi D, Ebisawa M, Kakiguchi K, Yonemura S, Jinnohara T, Kanaya T, Fujimura Y, Ohmae M, Hase K, Ohno H.
In vivo notch reactivation in differentiating cochlear hair cells induces sox2 and prox1 expression but does not disrupt hair cell maturation. Dev Dyn.241(4): 684-96 (2012).(22354878)
Liu Z, Owen T, Fang J, Srinivasan RS, Zuo J.
Notch signaling in chondrocytes modulates endochondral ossification and osteoarthritis development. Proc Natl Acad Sci U S A. (2013).(23319657)
Hosaka Y, Saito T, Sugita S, Hikata T, Kobayashi H, Fukai A, Taniguchi Y, Hirata M, Akiyama H, Chung UI, Kawaguchi H.
Epithelial-stromal interaction via Notch signaling is essential for the full maturation of gut-associated lymphoid tissues. EMBO Rep.15(12): 1297-304 (2014).(25378482)
Obata Y, Kimura S, Nakato G, Iizuka K, Miyagawa Y, Nakamura Y, Furusawa Y, Sugiyama M, Suzuki K, Ebisawa M, Fujimura Y, Yoshida H, Iwanaga T, Hase K, Ohno H.
A Genome-Wide Analysis Identifies a Notch-RBP-J kappa-IL-7R alpha Axis That Controls IL-17-Producing gamma delta T Cell Homeostasis in Mice. J Immunol. (2014).(25429074)
Nakamura M, Shibata K, Hatano S, Sato T, Ohkawa Y, Yamada H, Ikuta K, Yoshikai Y.
O-GlcNAc on NOTCH1 EGF repeats regulates ligand-induced Notch signaling and vascular development in mammals. Elife6: (2017).(28395734)
Sawaguchi S, Varshney S, Ogawa M, Sakaidani Y, Yagi H, Takeshita K, Murohara T, Kato K, Sundaram S, Stanley P, Okajima T.

RBRC06296, CAJcl.Cg-Aicda<tm2.1Hon>

RBRC No. RBRC06296
Type Targeted MutationCartagena
Species Mus musculus
Strain name CAJcl.Cg-Aicda<tm2.1Hon>
Former Common name BALBcAJcl-AID G23S, BALBcA-AID/G23S KI
H-2 Haplotype No Data
ES cell line Bruce 4 [B6.Cg-Thy1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Reiko Shinkura, Nagahama Institute of Bio-Science and Technology, and Tasuku Honjo, Graduate School of Medicine, Kyoto University. Bruce 4 ES cells were used. The mutant mice were backcrossed to BALB/cAJcl.
Strain description Aicda G23S mutant knock-in mice. BALB/cAJcl background. For the details, please contact the developer (Dr. Reiko Shinkura, Nagahama Institute of Bio-Science and Technology).
Colony maintenance Homozygote x Homozygote [or Crossing to BALB/cAJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Aicda
Symbol name activation-induced cytidine deaminase
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
1) The RECIPIENT shall use the BIOLOGICAL RESOURCE only for academic research for the purpose of publishing the research results.
2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a citation of the following literature(s) designated by the DEPOSITOR are requested. Nat. Immunol., 12, 264-270 (2011).
3) RECIPIENT shall notify the PROVIDER upon filing a patent application claiming modification of the BIOLOGICAL RESOURCE or method(s) of manufacture or use(s) of the BIOLOGICAL RESOURCE.
Additional information
1 Honjo Lab HP
Depositor Honjo, Tasuku (Graduate School of Medicine, Kyoto University) Honjo, Tasuku
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months