RBRC01188, B6.Cg-Tg(CTSG-NUP98/HOXA9)1589Tanak/TanakRbrc

RBRC No. RBRC01188
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(CTSG-NUP98/HOXA9)1589Tanak/TanakRbrc
Former Common name t(7;11)AML NUP98-HOXA9 transgenic (Tg) mice
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Kakuro Nakamura, NIH in 1996. The transgene was injected into the pronuclei of B6D2F2 fertilized eggs. The transgenic mice were backcrossed to C57BL/6J over 10 times.
Strain description B6.Cg-Tg(CTSG-NUP98/HOXA9)1589Tanak. This transgenic mice express the chimeric NUP98-HOXA9 gene in the myeloid lineage. About 20% of mice with this transgene exhibit myeloproliferative disorder and acute myeloid leukemia (AML).
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol CTSG
Symbol name Cathepsin G (human)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol HOXA9
Symbol name homeo box A9 (human)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter human Cathepsin G promoter
3 Symbol NUP98
Symbol name nucleoporin 98 (human)
Chromosome UN
Common name No Data
Symbol description No Data
References Blood 2005 Jan 15;105(2):784-93. Epub 2004 Sep 28.

Identification of cooperative genes for NUP98-HOXA9 in myeloid leukemogenesis using a mouse model.
Research applications Cancer Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Additional information
1 The Cancer Institute of JFCR
2 Mouse of the Month Mar 2006
3 Genotyping protocol <PCR>
Depositor Nakamura, Takuro (Japanese Foundation for Cancer Research) Nakamura, Takuro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
RNA export factor RAE1 contributes to NUP98-HOXA9-mediated leukemogenesis. Cell Cycle.10(9): 1456-67 (2011).(21467841)
Funasaka T, Nakano H, Wu Y, Hashizume C, Gu L, Nakamura T, Wang W, Zhou P, Moore MA, Sato H, Wong RW.

RBRC01189, CB.Cg-Tg(ITGAL-BCL11A)/Rbrc

RBRC No. RBRC01189
Type TransgeneCartagena wks
Species Mus musculus
Strain name CB.Cg-Tg(ITGAL-BCL11A)/Rbrc
Former Common name Evi9/BclIIa transgenic mice
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol CAPG
Symbol name capping protein (actin filament), gelsolin-like (human)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter human ITGAL(CD11a) promoter
2 Symbol BCL11A
Symbol name B-cell CLL/lymphoma 11A (zinc finger protein)
Chromosome UN
Common name 2810047E18Rik, COUP-TF interacting protein 1, CTIP1, D930021L15Rik, Evi9, mKIAA1809, mouse myeloid leukemia gene
Symbol description No Data
References Unpublished

Research applications Cancer Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Additional information
1 The Cancer Institute of JFCR
2 Genotyping protocol <PCR>
Depositor Nakamura, Takuro (Japanese Foundation for Cancer Research) Nakamura, Takuro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC01190, 129-Bcl11a<tm1Ngc>/Rbrc

RBRC No. RBRC01190
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name 129-Bcl11a<tm1Ngc>/Rbrc
Former Common name Evi9/BclIIa Knockout mice
H-2 Haplotype No Data
ES cell line CJ7 [129/SvlmJ]
Donor strain 129S1/SvImJ via CJ7 ES cell line
Background strain No Data
Appearance
1 Appearance white-bellied agouti
Genotype A/A B/B C/C
Strain development Developed by Drs. Pentao Liu and Neal G Copeland at NIH in 1999. A PGK-neo cassette replaced was transfered into CJ7 ES cells to replace exon 1 of Bcl11a gene.
Strain description Developed by Drs. Pentao Liu and Neal G Copeland at NIH in 1999. A PGK-neo cassette replaced was transfered into CJ7 ES cells to replace exon 1 of Bcl11a gene.
Colony maintenance Sibling Mating (Heterozygote x Wild-type, Heterozygote)
Homozygous mutant mice die within 3-4hrs after birth.
Health Report
Gene Details
Promoter No Data
1 Symbol Bcl11a
Symbol name B-cell CLL/lymphoma 11A (zinc finger protein)
Chromosome 11
Common name 2810047E18Rik, 4930538E20Rik, COUP-TF interacting protein 1, CTIP1, Evi9, mKIAA1809, mouse myeloid leukemia gene
Symbol description No Data
2 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 11
Common name No Data
Symbol description No Data
3 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 11
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References Nat Immunol. 2003 Jun;4(6):525-32. Epub 2003 Apr 28.

Bcl11a is essential for normal lymphoid development.
Research applications Fluorescent Proteins/lacZ System,
Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature Immunol., 6, 525-532 (2003).
Additional information
1 The Cancer Institute of JFCR
2 Genotyping protocol <PCR>
Depositor Nakamura, Takuro (Japanese Foundation for Cancer Research) Nakamura, Takuro
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC01191, B6;129-Meis1<tm1Ngc>/Rbrc

RBRC No. RBRC01191
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129-Meis1<tm1Ngc>/Rbrc
Former Common name Meis1 Knockout mice
H-2 Haplotype No Data
ES cell line JR2448 [129S3/SvImJ]
Donor strain 129S3/SvImJ via JR2448 ES cell line
Background strain C57BL/6J
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Drs. Masami Fujita and Neal Copeland, National Cancer Institute in 1997. Donor strain is 129S3/SvlmJ via JR2448 ES cell line. This strain was maintained in C57BL/6J and 129S3/SvlmJ mixed background.
Strain description B6;129S3-Meis1<tm1Ngc>. Meis1 deficient mice exhibit abnormal retina, vasculature, hematopoietic system defects. Homozygous mutant mice die by embryonic day 14.5.
Colony maintenance Litter size is a little small. Embryonic lethality (Homozygous).
Genotyping by PCR.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Meis1
Symbol name myeloid ecotropic viral integration site 1
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 11
Common name No Data
Symbol description No Data
3 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 11
Common name No Data
Symbol description E. coli beta galactosidase
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References EMBO J. 2004 Jan 28;23(2):450-9. Epub 2004 Jan 8.

Hematopoietic, angiogenic and eye defects in Meis1 mutant animals.
Research applications Fluorescent Proteins/lacZ System,
Sensorineural Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
EMBO J., 23, 450-459 (2004).
Additional information
1 The Cancer Institute of JFCR
2 Genotyping protocol <PCR>
Depositor Nakamura, Takuro (Japanese Foundation for Cancer Research) Nakamura, Takuro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01192, B6;129-Meis3<tm1Ngc>/Rbrc

RBRC No. RBRC01192
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129-Meis3<tm1Ngc>/Rbrc
Former Common name Meis3 Knockout mice
H-2 Haplotype No Data
ES cell line JR2448 [129S3/SvImJ]
Donor strain 129S3/SvImJ via JR2448 ES cell line
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Meis3
Symbol name Meis homeobox 3
Chromosome 7
Common name Mrg2
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 7
Common name No Data
Symbol description E. coli beta galactosidase
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 7
Common name neo; neomycin;
Symbol description No Data
References Unpublished

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Additional information
1 The Cancer Institute of JFCR
2 Genotyping protocol <PCR>
Depositor Nakamura, Takuro (Japanese Foundation for Cancer Research) Nakamura, Takuro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months