RBRC01861, C57BL/6-Tg(NodalNDE-Cre)16Hmd

RBRC No. RBRC01861
Type TransgeneCartagena wks
Species Mus musculus
Strain name C57BL/6-Tg(NodalNDE-Cre)16Hmd
Former Common name NDE-Cre
H-2 Haplotype No Data
Background strain C57BL/6NCrSlc
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Drs. Yukiko Saijoh and Hiroshi Hamada at Osaka University at 2000. The Nodal-cre plasmids were injected into the pronuclei of C57BL/6Cr fertilized eggs.
Strain description No Data
Colony maintenance Backcross to C57BL/6 (Hemizygote x C57BL/6CrSlc)
Health Report
Gene Details
Promoter mouse Nodal gene promoter, enhancer
1 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References Dev Biol. 2003 Apr 1;256(1):160-72.

Left-right patterning of the mouse lateral plate requires nodal produced in the node.
Research applications Cre/loxP system,
Developmental Biology Research,
General Purpose,
Genetics Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE.
Additional information
1 Mouse of the Month May 2007
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02444, B6.Cg-Tg(Lefty2 3.3-cre)77bHmd

RBRC No. RBRC02444
Type Transgene CongenicCartagena wks
Species Mus musculus
Strain name B6.Cg-Tg(Lefty2 3.3-cre)77bHmd
Former Common name B6-lefty2 3.3-Cre, B6;C3-Tg(lefty2 3.3-Cre)77b Hmd
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter Lefty2 lateral plate mesoderm enhancer and promoter
1 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04112, B6;129-Nodal<tm2Hmd>

RBRC No. RBRC04112
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6;129-Nodal<tm2Hmd>
Former Common name Nodal beta geo
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Nodal
Symbol name nodal
Chromosome 10
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04799, B6;B6129-EpCAM<tm1.1Hmd>

RBRC No. RBRC04799
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;B6129-EpCAM<tm1.1Hmd>
Former Common name EpCAM floxed
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University. The construct was electroporated into G4 ES cells derived from B6129F1. The SA-IRES-Bgeo was excised by crossing with mice expressing F1p (B6-FLPe transgenic mice).
Strain description Exon2 and exon3 of Tacstd1 (Epcam, epithelial cell adhesion molecule) gene flanked by loxp sites. Conditional Tacstd1 deficient mice can be generated by cyossing with tissue-specific Cre mice. Homozygous mice were viable and fertile.
Colony maintenance Heterozygote X Wild-type [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Epcam
Symbol name epithelial cell adhesion molecule
Chromosome 17
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 17
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 17
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05246, B6C3-Tg(Tcf-hsp-Venus)53Hmd

RBRC No. RBRC05246
Type TransgeneCartagena
Species Mus musculus
Strain name B6C3-Tg(Tcf-hsp-Venus)53Hmd
Former Common name B6C3-Tcf-Venus Tg
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Tetsuya Nakamura and Hiroshi Hamada, Institute for Molecular and Cellular Biology, Osaka University in 2009. The Tcfx4-hsp-Venus transgene was injected into the pronuclei of B6C3F1 fertilized eggs.
Strain description Tcf-venus reporter transgenic mice. These mice are useful for monitoring of Wnt canonical pathway signals.
Colony maintenance Carrier x Noncarrier [or Crossing to Jcl:B6C3F1]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name Sv40 polyA additional signal
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter mouse Hsp68 promoter
2 Symbol Venus
Symbol name Green Fluorescent Protein, F46L (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06207, B6C3-Tg(Cerl2-sGFP*d3)6Hmd

RBRC No. RBRC06207
Type TransgeneCartagena
Species Mus musculus
Strain name B6C3-Tg(Cerl2-sGFP*d3)6Hmd
Former Common name Cerl2(Cerl2-sGFP-Delta 3′)BAC(6)
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Tetsuya Nakamura and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2008. The transgene was injected into the pronuclei of B6C3F1 fertilized eggs. C57BL/6 and C3H mixed background.
Strain description BAC Cerl2-sGFP (delta 3') transgenic mice. The mice were generated by using a BAC clone containing the mouse Cerl2 gene, PR23-421H21. The sGFP (a 300-bp sequence coding for a portion of EGFP) gene was inserted into the exon 2 of in the Cerl2 BAC clone. The 3'-UTR of Cerl2 was replaced by the polyA sequence (250 bp) of SV40.
Colony maintenance Carrier x Noncarrier [or Crossing to Jcl:B6C3F1]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol polyA
Symbol name SV40 virus polyA signal sequence
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Dand5 promoter
2 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Developmental Biology Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC06554, B6.Cg-Tg(RAREn-hsp-lacZ)5Hmd

RBRC No. RBRC06554
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(RAREn-hsp-lacZ)5Hmd
Former Common name RAREn-hsp-lacZ(5), B6-RAREn-lacZ Tg#5
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Katsuyoshi Takaoka and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2012. The transgene was injected into the pronuclei of B6C3F2 fertilized eggs. C57BL/6 and C3H mixed background.
Strain description 9xRAREn-hsp-lacZ transgenic mice. Line 3 (RBRC06553), Line 5 (RBRC06554).
Colony maintenance Carrier x Noncarrier [or Crossing to C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Nodal
Symbol name mouse Nodal retinoic acid response element (RAREn)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse hsp68 promoter
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 No Data
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02106, B6.129-Lefty1<tm1Hmd>

RBRC No. RBRC02106
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129-Lefty1<tm1Hmd>
Former Common name lefty 1 null KO
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Lefty1
Symbol name left right determination factor 1
Chromosome 1
Common name Leftb, lefty-1, Stra3
Symbol description No Data
Promoter SV40 and herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 1
Common name neo; neomycin;
Symbol description No Data
References Cell. 1998 Aug 7;94(3):287-97.

lefty-1 is required for left-right determination as a regulator of lefty-2 and nodal.
Research applications Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE.
Additional information
1 Mouse of the Month May 2007
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02445, B6.Cg-Tg(Lefty2 5.5-cre)21BHmd

RBRC No. RBRC02445
Type Transgene CongenicCartagena wks
Species Mus musculus
Strain name B6.Cg-Tg(Lefty2 5.5-cre)21BHmd
Former Common name B6-lefty2 5.5-Cre, B6;C3-Tg(lefty2 5.5-Cre)21B Hmd
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter Lefty2 epiblast enhancer and promoter
1 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References Genes Dev. 2001 May 15;15(10):1242-56.

The transcription factor FoxH1 (FAST) mediates Nodal signaling during anterior-posterior patterning and node formation in the mouse.
Research applications Cre/loxP system,
Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04113, B6;129-Nodal<tm2.1Hmd>

RBRC No. RBRC04113
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129-Nodal<tm2.1Hmd>
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Nodal
Symbol name nodal
Chromosome 10
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months