RBRC06200, B6C3-Tg(Nodal-lacZ)2yHmd

RBRC No. RBRC06200
Type TransgeneCartagena
Species Mus musculus
Strain name B6C3-Tg(Nodal-lacZ)2yHmd
Former Common name Nodal-lacZ/BAC(2y), Nodal-lacZ Tg#2y
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Katsuyoshi Takaoka, Masayuki Uehara and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2008. The transgene was injected into the pronuclei of B6C3F1 fertilized eggs. C57BL/6 and C3H mixed background.
Strain description Nodal-lacZ transgenic mice. The transgene may be integrated into chromosome Y.
Colony maintenance Carrier (male) x Noncarrier (female) [or Crossing to Jcl:B6C3F1]
The transgene may be integrated into chromosome Y.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome Y
Common name No Data
Symbol description No Data
Promoter mouse Nodal promoter
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome Y
Common name No Data
Symbol description No Data
References

Research applications Developmental Biology Research,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06547, B6.Cg-Prickle2<tm1Hmd>

RBRC No. RBRC06547
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Prickle2<tm1Hmd>
Former Common name Prickle2 flox(neo)#60, B6-Prickle2/fneo
H-2 Haplotype No Data
ES cell line G4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Masakazu Hashimoto, Katsura Minegishi and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2010. G4 ES cells derived from (129 x C57BL/6)F1 were used to generate the mutant mice. C57BL/6 and 129 mixed background.
Strain description Prickle1 gene floxed neo mice. Exon 6 was flanked by loxP sites. A frt flanked PGK-neo cassette was inserted after the exon 6.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name polyA
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol Prickle2
Symbol name prickle homolog 2 (Drosophila)
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 No Data
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02314, B6;129-Cyp26b1<tm1Hh>

RBRC No. RBRC02314
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6;129-Cyp26b1<tm1Hh>
Former Common name Cyp26 b1 null
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Cyp26b1
Symbol name cytochrome P450, family 26, subfamily b, polypeptide 1
Chromosome 6
Common name CP26, P450RAI-2, retinoic acid B1
Symbol description No Data
2 Symbol poly A
Symbol name HSV TK poly A
Chromosome 6
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol puro
Symbol name puromycin resistance gene (Streptomyces alboniger)
Chromosome 6
Common name No Data
Symbol description No Data
References Dev Cell. 2004 Mar;6(3):411-22.

Regulation of retinoic acid distribution is required for proximodistal patterning and outgrowth of the developing mouse limb.
Research applications Developmental Biology Research,
Metabolism Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC03827, STOCK Tg(Foxa2mNE-hsp-Pkd2/Venus)75Hmd

RBRC No. RBRC03827
Type TransgeneCartagena
Species Mus musculus
Strain name STOCK Tg(Foxa2mNE-hsp-Pkd2/Venus)75Hmd
Former Common name Foxa2mNE-hsp-Pkd2/Venus(75); Foxa2 mNE-Pkd2-Venus(75); HNF3βmNE-Pkd2-Venus(75)
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Satoko Yoshiba and Dr. Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2007.
Strain description These transgenic mice express the Pkd2/venus gene in node under the control of the mouse HNF3beta node/notochordal specific enhancer.
Health Report
Gene Details
Promoter No Data
1 Symbol Venus
Symbol name Green Fluorescent Protein, F46L (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Foxa2 enhancer, hsp promoter
2 Symbol Pkd2
Symbol name polycystic kidney disease 2
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04703, B6;129B6-Pih1d3<tm1Hmd>

RBRC No. RBRC04703
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129B6-Pih1d3<tm1Hmd>
Former Common name Twister (flox/+)
H-2 Haplotype No Data
ES cell line G4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2006. A PGK-neo cassette was removed by crossing with CAG-FLPe mice.
Strain description Pih1d3 (Stp/Twister) gene floxed mice. Expression of Pih1d3 is detected by X-gal staining.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Pih1d3
Symbol name PIH1 domain containing 3
Chromosome 1
Common name Sfp; sperm flagellar protein
Symbol description No Data
2 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 1
Common name internal ribosomal entry site from EMCV
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 1
Common name No Data
Symbol description No Data
4 Symbol Myc-tag
Symbol name myc-epitope tag
Chromosome 1
Common name No Data
Symbol description No Data
5 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 1
Common name No Data
Symbol description E. coli beta galactosidase
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 1
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05058, B6;B6C3(129)-Tg(Cerl1-cre/ERT2)1Hmd

RBRC No. RBRC05058
Type TransgeneCartagena
Species Mus musculus
Strain name B6;B6C3(129)-Tg(Cerl1-cre/ERT2)1Hmd
Former Common name Cer1-creERT2/BAC (1) B6-Cer1CreER Tg#1
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroshi Hamada, Institute for Molecular and Cellular Biology, Osaka University in 2009. The transgene was injected into the pronuclei of B6C3F1 fertilized egg.
Strain description No Data
Colony maintenance Carrier x Carrier, Noncarrier [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol ERT2
Symbol name estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Cerbelus-like1 enhancer
2 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06201, B6C3-Tg(Nodal-lacZ)3Hmd

RBRC No. RBRC06201
Type TransgeneCartagena
Species Mus musculus
Strain name B6C3-Tg(Nodal-lacZ)3Hmd
Former Common name Nodal-lacZ/BAC(3), Nodal-lacZ Tg#3
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Katsuyoshi Takaoka, Masayuki Uehara and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2008. The transgene was injected into the pronuclei of B6C3F1 fertilized eggs. C57BL/6 and C3H mixed background.
Strain description Nodal-lacZ transgenic mice. The transgene may be integrated into chromosome Y.
Colony maintenance Carrier x Noncarrier [or Crossing to Jcl:B6C3F1]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Nodal promoter
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Developmental Biology Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06548, B6.Cg-Prickle2<tm1.2Hmd>

RBRC No. RBRC06548
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Prickle2<tm1.2Hmd>
Former Common name Prickle2 delta ex6, B6-Prickle2 KO
H-2 Haplotype No Data
ES cell line G4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Masakazu Hashimoto, Katsura Minegishi and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2010. G4 ES cells derived from (129 x C57BL/6)F1 were used to generate the mutant mice. Flanked Exon 6 was removed by crossing with CAG-Cre mice (B6/129 background). A frt flanked PGK-neo cassette was removed by crossing with CAG-FLPe mice (B6/129 background). C57BL/6 and 129 mixed background.
Strain description Exon 6 of Prickle2 gene was removed.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol Prickle2
Symbol name prickle homolog 2 (Drosophila)
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 No Data
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02405, B6.Cg-4930583H14Rik<tm1Hmd>

RBRC No. RBRC02405
Type Targeted Mutation CongenicCartagena wks
Species Mus musculus
Strain name B6.Cg-4930583H14Rik<tm1Hmd>
Former Common name 4930583H14Rik flox
H-2 Haplotype No Data
ES cell line G4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takako Maeda and Hiroshi Hamada, Institute for Molecular and Cellular Biology, Osaka University in 2007. G4 ES cells derived from (129S6 x C57BL/6)F1 were used. C57BL/6 and 129 mixed background.
Strain description 4930583H14Rik floxed mice. ORF region of the exon 4 was flanked by loxP sites.
Health Report
Gene Details
Promoter No Data
1 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 3
Common name internal ribosomal entry site from EMCV
Symbol description No Data
2 Symbol 4930583H14Rik
Symbol name RIKEN cDNA 4930583H14 gene
Chromosome 3
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 3
Common name No Data
Symbol description No Data
4 Symbol Myc-tag
Symbol name myc-epitope tag
Chromosome 3
Common name No Data
Symbol description No Data
5 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 3
Common name No Data
Symbol description E. coli beta galactosidase
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 3
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
7 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 3
Common name neo; neomycin;
Symbol description No Data
References No Data

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC03828, STOCK Tg(Lefty1 0.7-lacZ)3Hmd

RBRC No. RBRC03828
Type TransgeneCartagena
Species Mus musculus
Strain name STOCK Tg(Lefty1 0.7-lacZ)3Hmd
Former Common name Lefty1-0.7-lacZ(3)
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome No Data
Common name No Data
Symbol description No Data
References Dev Cell. 2006 Apr;10(4):451-9.

The mouse embryo autonomously acquires anterior-posterior polarity at implantation.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months