RBRC01544, B6.129P2-Sirpa<tm1Nog>/Rbrc

RBRC No. RBRC01544
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129P2-Sirpa<tm1Nog>/Rbrc
Former Common name SHPS-1 Knockout mice
H-2 Haplotype No Data
ES cell line E14.1 [129P2/OlaHsd]
Donor strain 129P2/OlaHsd via E14.1 ES cell line
Background strain C57BL/6NJcl
Appearance
1 Appearance black
Genotype No Data
Strain development Developed by Dr. Kenjiro Inagaki, Kobe University School of Medicine in 2000. The construct was electoroporated into E14.1 ES cells derived from 129P2/OlaHsd. The mutant mice were backcrossed C57BL/6N over 10 times.
Strain description SHPS-1 knockout mice. Exon 7 and 8 were replaced with a nemoycin selection cassette. SHPS-1 (Ptpns1, protein tyrosine phosphatase, non-receptor type substrate 1) gene ia a member of sinal-regulatory proteins, plays a impotant roles in integrin-mediated cytoskeletal reoganization, cell motility and the regulation of Rho.
Colony maintenance Heterozygote x Wild-type [C57BL/6NJcl]. Homozygous mutant mice are viable and fertile.
Health Report
Gene Details
Promoter No Data
1 Symbol Sirpa
Symbol name signal-regulatory protein alpha
Chromosome 2
Common name Bit, CD172a, P84, SHPS-1, SIRP; Ptpns1;protein tyrosine phosphatase, non-receptor type substrate 1
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 2
Common name neo; neomycin;
Symbol description No Data
References EMBO J 2000 Dec 15;19(24):6721-31.

SHPS-1 regulates integrin-mediated cytoskeletal reorganization and cell motility.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must submit its receipt to the DEPOSITOR immediately after receiving the BIOLOGICAL RESOURCE.
Additional information
1 Genetic Background
2 Lab HP
3 Genotyping protocol <PCR>
Depositor Matozaki, Takashi (Kobe University) Matozaki, Takashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Signal Inhibitory Regulatory Protein Alpha Influences Macrophage Clearance From The Alveolar Space After An Inflammatory Stimulus. Am. J. Respir. Crit. Care Med.181: A2761 (2010).
Mark Kearns, William J. Janssen, and Peter M. Henson.
SHPS-1 deficiency induces robust neuroprotection against experimental stroke by attenuating oxidative stress. J Neurochem.122(4): 834-843 (2012).(22671569)
Wang L, Lu Y, Deng S, Zhang Y, Yang L, Guan Y, Matozaki T, Ohnishi H, Jiang H, Li H.
Increased Susceptibility to Salmonella Infection in Signal Regulatory Protein alpha-Deficient Mice. J Immunol.189(5): 2537-44 (2012).(22851710)
Li LX, Atif SM, Schmiel SE, Lee SJ, McSorley SJ.

RBRC02650, B6;129S4-Ptprh<tm1>

RBRC No. RBRC02650
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129S4-Ptprh<tm1>
Former Common name Sap-1 loxed
H-2 Haplotype No Data
ES cell line J1 [129S4/SvJae]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 7
Common name neo; neomycin;
Symbol description No Data
2 Symbol Ptprh
Symbol name protein tyrosine phosphatase, receptor type, H
Chromosome 7
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 7
Common name No Data
Symbol description E. coli beta galactosidase
References No Data

Research applications Cell Biology Research,
Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Additional information
1 Lab HP
Depositor Matozaki, Takashi (Kobe University) Matozaki, Takashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02651, B6.Cg-Ptprh<tm1.1>

RBRC No. RBRC02651
Type Targeted Mutation CongenicCartagena wks
Species Mus musculus
Strain name B6.Cg-Ptprh<tm1.1>
Former Common name Sap-1 KO
H-2 Haplotype No Data
ES cell line J1 [129S4/SvJae]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Ptprh
Symbol name protein tyrosine phosphatase, receptor type, H
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Matozaki, Takashi (Kobe University) Matozaki, Takashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04488, C.129P2(B6)-Sirpa<tm1Nog>

RBRC No. RBRC04488
Type Targeted MutationCartagena
Species Mus musculus
Strain name C.129P2(B6)-Sirpa<tm1Nog>
Former Common name SIRP alpha mutant mice
H-2 Haplotype No Data
Background strain BALB/cAJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Sirpa
Symbol name signal-regulatory protein alpha
Chromosome 2
Common name Bit, CD172a, P84, SHPS-1, SIRP; Ptpns1;protein tyrosine phosphatase, non-receptor type substrate 1
Symbol description No Data
References EMBO J 2000 Dec 15;19(24):6721-31.

SHPS-1 regulates integrin-mediated cytoskeletal reorganization and cell motility.
Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT f BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Matozaki, Takashi (Kobe University) Matozaki, Takashi
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months