RBRC04608, B6;129P2-Runx1<tm1Homy>

RBRC No. RBRC04608
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Runx1<tm1Homy>
Former Common name Runx1-delta IRES
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Runx1
Symbol name runt related transcription factor 1
Chromosome 16
Common name AML1, Cbfa2, Pebp2a2, runt domain, alpha subunit 2
Symbol description No Data
2 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome 16
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 16
Common name No Data
Symbol description No Data
4 Symbol Ires
Symbol name internal ribosomal entry site (EMCV)
Chromosome 16
Common name No Data
Symbol description No Data
5 Symbol RUNX1
Symbol name runt related transcription factor 1(human)
Chromosome 16
Common name No Data
Symbol description No Data
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 16
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05655, B6;129P2-Cbl<tm1(CBL*Q367P)Homy>

RBRC No. RBRC05655
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Cbl<tm1(CBL*Q367P)Homy>
Former Common name Cbl Q367P cKI, B6-Cbl/CBL*Q367P KI
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2012. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6 mice. Neomycin selection cassette was removed by crossing with FLPe mice.
Strain description Exon 5 to 9 of Cbl (Casitas B-lineage lymphoma) gene was replaced with a loxP flanked human CBL, and a human CBL having Q367P variant was inserted the downstream.
Colony maintenance Heterozygote x Wild-type [C57BL/6NCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol CBL
Symbol name Casitas B-lineage lymphoma(human)
Chromosome 9
Common name No Data
Symbol description No Data
2 Symbol Cbl
Symbol name Casitas B-lineage lymphoma
Chromosome 9
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 9
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 9
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 9
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04609, B6;129P2-Wnt5a<tm1.1Homy>

RBRC No. RBRC04609
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Wnt5a<tm1.1Homy>
Former Common name Wnt5a-floxed
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 14
Common name No Data
Symbol description No Data
2 Symbol Wnt5a
Symbol name wingless-related MMTV integration site 5A
Chromosome 14
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 14
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 14
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
Developmental Biology Research,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
WNT5a is required for normal ovarian follicle development and antagonizes gonadotropin responsiveness in granulosa cells by suppressing canonical WNT signaling. FASEB J.30: 1534-1547 (2016).(26667040)
Abedini A, Zamberlam G, Lapointe E, Tourigny C, Boyer A, Paquet M, Hayashi K, Honda H, Kikuchi A, Price C, Boerboom D.

RBRC05921, B6;129P2-Eed<tm1(L240Q)Homy>

RBRC No. RBRC05921
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Eed<tm1(L240Q)Homy>
Former Common name Eed L240Q KI mice
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2012. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6 mice. Neomycin selection cassette was removed by crossing with Cre mice.
Strain description Eed L240Q mutant knock-in mice. L240Q mutation in the EED gene was found from patients with myelodysplastic syndromes.
Colony maintenance Heterozygote x Wild-type [C57BL/6NCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol Eed
Symbol name embryonic ectoderm development
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Developmental Biology Research,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04841, B6N.129P2-Bhlhe40<tm1Homy>

RBRC No. RBRC04841
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6N.129P2-Bhlhe40<tm1Homy>
Former Common name Dec1 KO
H-2 Haplotype No Data
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Bhlhe40
Symbol name basic helix-loop-helix family, member e40
Chromosome 6
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (Streptomyces fradiase)
Chromosome 6
Common name neo; neomycin;
Symbol description No Data
References

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Icariin protects against glucocorticoid induced osteoporosis, increases the expression of the bone enhancer DEC1 and modulates the PI3K/Akt/GSK3 beta/beta-catenin integrated signaling pathway. Biochem. Pharmacol.136: 109-121 (2017).(28408345)
Hu J, Mao Z, He S, Zhan Y, Ning R, Liu W, Yan B, Yang J.

RBRC05922, B6;129P2-Eed<tm1(I363M)Homy>

RBRC No. RBRC05922
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Eed<tm1(I363M)Homy>
Former Common name IEed I363M K
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2012. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6 mice. Neomycin selection cassette was removed by crossing with Cre mice.
Strain description Eed I363M mutant knock-in mice. I363M mutation in the EED gene was found from patients with myelodysplastic syndromes.
Colony maintenance Heterozygote x Wild-type [C57BL/6NCrlCrlj]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Eed
Symbol name embryonic ectoderm development
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Developmental Biology Research,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04842, C57BL/6N-Tg(Lck-Bhlhe40)1Homy

RBRC No. RBRC04842
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6N-Tg(Lck-Bhlhe40)1Homy
Former Common name Dec1 Tg
H-2 Haplotype No Data
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol FLAG
Symbol name FLAG tag (synthetic)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Lck promoter
2 Symbol Bhlhe40
Symbol name basic helix-loop-helix family, member e40
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05923, B6;129P2-Eed<tm1Homy>

RBRC No. RBRC05923
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Eed<tm1Homy>
Former Common name Eed cKO mice
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2012. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6 mice. Neomycin selection cassette was removed by crossing with FLPe mice.
Strain description Eed floxed mice. Exon 6 of the Eed gene was flanked by loxP sites.
Colony maintenance Heterozygote x Wild-type [C57BL/6NCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol Eed
Symbol name embryonic ectoderm development
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 7
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Developmental Biology Research,
FLP/frt system,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05198, B6;129P2-Ciz1<tm1Homy>

RBRC No. RBRC05198
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Ciz1<tm1Homy>
Former Common name Ciz1 cKO B6-Ciz1 floxed
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2008. A loxP was inserted upstream of exon 5 and an FRT flanked neo cassette with a 3' loxP site was inserted downstream of exon 5. Neo cassette was removed by crossing with FLPe mice (C57BL/6-Tg (CAG-FLPe) 36Ito). The mice were backcrossed with C57BL/6N for at least 6 times.
Strain description Ciz1 gene floxed mice. Exon 5 of Ciz1 gene is flanked by loxP sites. Conditonal Ciz1 deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenance Heterozygote X Wild-type [C57BL/6NCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol Ciz1
Symbol name CDKN1A interacting zinc finger protein 1
Chromosome 2
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome No Data
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome No Data
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05953, B6;129P2-Tg(Ly6a-DR-GFP)1Homy

RBRC No. RBRC05953
Type TransgeneCartagena
Species Mus musculus
Strain name B6;129P2-Tg(Ly6a-DR-GFP)1Homy
Former Common name Sca1-DR-GFP, B6-Ly6a-DR-GFP Tg
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2011. The mutant mice were crossed to C57BL/6 mice.
Strain description ScaI(Ly6A)-DR-GFP tragsnegnic mice. Homology-directed repair (HDR) reporter DR-GFP mice under control of the mouse Ly6a promoter.
Colony maintenance Carrier x Noncarrier [C57BL/6NCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol Sce-GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol iGFP
Symbol name intense GFP(iGFP) (Aequorea victoria)
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months