RBRC04842, C57BL/6N-Tg(Lck-Bhlhe40)1Homy

RBRC No. RBRC04842
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6N-Tg(Lck-Bhlhe40)1Homy
Former Common name Dec1 Tg
H-2 Haplotype No Data
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol FLAG
Symbol name FLAG tag (synthetic)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Lck promoter
2 Symbol Bhlhe40
Symbol name basic helix-loop-helix family, member e40
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05923, B6;129P2-Eed<tm1Homy>

RBRC No. RBRC05923
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Eed<tm1Homy>
Former Common name Eed cKO mice
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2012. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6 mice. Neomycin selection cassette was removed by crossing with FLPe mice.
Strain description Eed floxed mice. Exon 6 of the Eed gene was flanked by loxP sites.
Colony maintenance Heterozygote x Wild-type [C57BL/6NCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol Eed
Symbol name embryonic ectoderm development
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 7
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Developmental Biology Research,
FLP/frt system,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05198, B6;129P2-Ciz1<tm1Homy>

RBRC No. RBRC05198
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Ciz1<tm1Homy>
Former Common name Ciz1 cKO B6-Ciz1 floxed
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2008. A loxP was inserted upstream of exon 5 and an FRT flanked neo cassette with a 3' loxP site was inserted downstream of exon 5. Neo cassette was removed by crossing with FLPe mice (C57BL/6-Tg (CAG-FLPe) 36Ito). The mice were backcrossed with C57BL/6N for at least 6 times.
Strain description Ciz1 gene floxed mice. Exon 5 of Ciz1 gene is flanked by loxP sites. Conditonal Ciz1 deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenance Heterozygote X Wild-type [C57BL/6NCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol Ciz1
Symbol name CDKN1A interacting zinc finger protein 1
Chromosome 2
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome No Data
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome No Data
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05953, B6;129P2-Tg(Ly6a-DR-GFP)1Homy

RBRC No. RBRC05953
Type TransgeneCartagena
Species Mus musculus
Strain name B6;129P2-Tg(Ly6a-DR-GFP)1Homy
Former Common name Sca1-DR-GFP, B6-Ly6a-DR-GFP Tg
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2011. The mutant mice were crossed to C57BL/6 mice.
Strain description ScaI(Ly6A)-DR-GFP tragsnegnic mice. Homology-directed repair (HDR) reporter DR-GFP mice under control of the mouse Ly6a promoter.
Colony maintenance Carrier x Noncarrier [C57BL/6NCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol Sce-GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol iGFP
Symbol name intense GFP(iGFP) (Aequorea victoria)
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05199, B6;129P2-Kdm2b<tm1Homy>

RBRC No. RBRC05199
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Kdm2b<tm1Homy>
Former Common name Fbxl10 cKO B6-Kdm2b floxed
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. A loxP was inserted upstream of exon 8 and an FRT flanked neo cassette with a 3' loxP site was inserted downstream of exon 8. Neo cassette was removed by crossing with FLPe mice (C57BL/6-Tg (CAG-FLPe) 36Ito). The mice were backcrossed with C57BL/6N for at least 8 times.
Strain description Kdm2b (Fbxl10) gene floxed mice. Exon 8 of Kdm2b gene is flanked by loxP sites. Conditonal Kdm2b deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenance Heterozygote X Wild-type [C57BL/6NCrlCrlj]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Kdm2b
Symbol name lysine (K)-specific demethylase 2B
Chromosome 5
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 5
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 5
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC10071, C57BL/6-Tg(S100A8-FCGR2A)1Homy

RBRC No. RBRC10071
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(S100A8-FCGR2A)1Homy
Former Common name MRP8/hFcgRIIa WT Tg
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome UN
Common name No Data
Symbol description No Data
Promoter human MRP8 promoter
2 Symbol FCGR2A
Symbol name No Data
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05200, B6;129P2-Kdm2b<tm1.1Homy>

RBRC No. RBRC05200
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Kdm2b<tm1.1Homy>
Former Common name Fbxl10 KO B6-Kdm2b KO
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. A loxP was inserted upstream of exon 8 and an FRT flanked neo cassette with a 3' loxP site was inserted downstream of exon 8. Exon 8 was removed by crossing with Cre mice (B6. B6C3-Tg (CAG-Cre) CZ-M02Osb). The mice were backcrossed with C57BL/6N for at least 9 times.
Strain description Kdm2b (Fbxl10) gene knockout mice. Exon 8 was removed by cre-mediated recombination.
Colony maintenance Heterozygote X Wild-type [C57BL/6NCrlCrlj]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Kdm2b
Symbol name lysine (K)-specific demethylase 2B
Chromosome 5
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome No Data
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome No Data
Common name neo; neomycin;
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC10072, C57BL/6-Tg(S100A8-FCGR2A<*>A225I)1Homy

RBRC No. RBRC10072
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(S100A8-FCGR2A<*>A225I)1Homy
Former Common name MRP8/hFcgRIIa A225I Tg
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome UN
Common name No Data
Symbol description No Data
Promoter human MRP8 promoter
2 Symbol FCGR2A
Symbol name No Data
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02293, B6.129P2-p130Cas<tm1.1Homy>

RBRC No. RBRC02293
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-p130Cas<tm1.1Homy>
Former Common name Cas-ex2<flox/flox>
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2003.
Strain description No Data
Colony maintenance backcross to C57BL/6N (Heterozygote x C57BL/6NCrlCrlj)
Health Report
Gene Details
Promoter No Data
1 Symbol Bcar1
Symbol name breast cancer anti-estrogen resistance 1
Chromosome 8
Common name Cas, Crkas, p130 Cas
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 8
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 8
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 8
Common name No Data
Symbol description No Data
References Genes Cells. 2008 Feb;13(2):145-57.

Functional analysis of Src homology 3-encoding exon (exon 2) of p130Cas in primary fibroblasts derived from exon 2-specific knockout mice.
Research applications Cell Biology Research,
Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05201, B6;129P2-Mbtd1<tm1Homy>

RBRC No. RBRC05201
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Mbtd1<tm1Homy>
Former Common name Hemp KO B6-Mbtd1 KI
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. The Mbtd1 mutant mice were generated by replacing the mbt-coding exons with IRES-GFP and floxed neo resistant gene. The neo resistant gene was removed by microinjecting a Cre expression vector into fertilized eggs. The mutant mice were backcrossed with C57BL/6N for at least 10 times.
Strain description Mbtd1 (Hemp) gene knockout mice. Exon 6, 7 and 8 (mbt-coding exons) were replaced with an IRES-GFP. Western blot analysis showed absence of Mbtb1 protein. Homozygous null mutant mice show skeletal abnormalities and are neonatal lethal.
Colony maintenance Heterozygote X Wild-type [C57BL/6NCrlCrlj]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Mbtd1
Symbol name mbt domain containing 1
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome No Data
Common name internal ribosomal entry site from EMCV
Symbol description No Data
3 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome No Data
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome No Data
Common name No Data
Symbol description No Data
References

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months