RBRC01708, B6.Cg-Tg(Mt1-RET)242Ina

RBRC No. RBRC01708
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(Mt1-RET)242Ina
Former Common name 242 RET
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Masashi Kato, Nagoya University Graduate School of Medicine. The Mt1-RET transgene was injected into the fertilized eggs of (BALB/c x C57BL/6) x BALB/c mice. C57BL/6 and BALB/c mixed background.
Strain description Mt1-Ret 242 transgenic mice. The mice express the human RET under the control of the mouse MT1 promoter/enhancer. Mt1-RET homozygous mutant mice are embryonic lethal.
Colony maintenance Carrier x Noncarrier
Health Report
Gene Details
Promoter No Data
1 Symbol Tg(Mt1-RET)242Ina
Symbol name transgene insertion 242, Izumi Nakashima
Chromosome UN
Common name Tg(Mt1-RFP/FET)242Num
Symbol description No Data
2 Symbol No Data
Symbol name SV40 splicing signal-polyadenylation site
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse metallothionein 1 promoter/enhancer
3 Symbol RET
Symbol name recombinant RET proto-oncogene (human)
Chromosome UN
Common name RET51, RET9
Symbol description No Data
References EMBO J. 1991 Nov;10(11):3167-75.

Aberrant melanogenesis and melanocytic tumour development in transgenic mice that carry a metallothionein/ret fusion gene.
Research applications Cancer Research,
Dermatology Research,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Kato, Masashi (Chubu University College of Life and Health Sciences) Kato, Masashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC01709, B6.Cg-Tg(Mt1-RET)304Ina/B6

RBRC No. RBRC01709
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(Mt1-RET)304Ina/B6
Former Common name 304-B6 RET
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C +/+
2 Appearance black with black skin (hyperpigmented skin)
Genotype a/a B/B C/C Tg/+
Strain development Developed by Masashi Kato, Nagoya University Graduate School of Medicine. The Mt1-RET transgene was injected into the fertilized eggs of (BALB/c x C57BL/6) x BALB/c mice. C57BL/6 and BALB/c mixed background.
Strain description Mt1-Ret 304 transgenic mice. The mice express the human RET under the control of the mouse MT1 promoter/enhancer. This Mt1-RET 304 strain is a model of cutaneous melanosis, and develops benign tumors 100% on the skin. Of these tumors, 65% transform to malignant tumors in ten to twelve months after the birth. Tg homozygous mice are embryonic lethal.
Colony maintenance Carrier x Noncarrier
Health Report
Gene Details
Promoter No Data
1 Symbol Tg(Mt1-RET)304Ina
Symbol name transgene insertion 304, Izumi Nakashima
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name SV40 splicing signal-polyadenylation site
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse metallothionein 1 promoter/enhancer
3 Symbol RET
Symbol name recombinant RET proto-oncogene (human)
Chromosome UN
Common name RET51, RET9
Symbol description No Data
References EMBO J. 1991 Nov;10(11):3167-75.

Aberrant melanogenesis and melanocytic tumour development in transgenic mice that carry a metallothionein/ret fusion gene.
Research applications Cancer Research,
Dermatology Research,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Kato, Masashi (Chubu University College of Life and Health Sciences) Kato, Masashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05847, B6.Cg-Ednrb<tm1Ywa> Tg(DBH-Ednrb)Ywa Tg(Dct-lacZ)A12Jkn

RBRC No. RBRC05847
Type Targeted Mutation and TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Ednrb<tm1Ywa> Tg(DBH-Ednrb)Ywa Tg(Dct-lacZ)A12Jkn
Former Common name Ednrb(-/-); DBH-Ednrb; Dct-LacZ+/+, B6-Ednrb KO/DBH-Ednrb/Dct-lacZ
H-2 Haplotype No Data
ES cell line JH1 [129S7/SvEvBrd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Masashi Kato, Nagoya University Graduate School of Medicine. C57BL/6 background. Ddnrb KO and DBH-Ednrb Tg alleles were generated by Masashi Yanagisawa, Howard Hughes Medical Institute. Dct-lacZ Tg allele was generated by Ian Jackson, Western General Hospital.
Strain description Ednrb KO/DBH-Ednrb Tg/Dct-lacZ Tg mice. Ednrb homozygous and DBH-Ednrb Tg mice are viable due to a lethal megacolon phenotype of Ednrb KO mice are rescued by the DBH-Ednrb expression. These mice also express lacZ in melanocytes under the control of Dct promoter.
Colony maintenance Ednrb KO : Homozygote x Heterozygote ; DBH-Ednrb : Carrier x Carrier ; Dct-lacZ : Carrier x Carrier (Homozygote x Homozygote)
Health Report
Gene Details
Promoter No Data
1 Symbol Ednrb
Symbol name endothelin receptor type B
Chromosome 14
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name SV40 splice and polyadenylation signals
Chromosome UN
Common name No Data
Symbol description No Data
Promoter human dopamine beta hydroxylase (DBH) promoter
3 Symbol Ednrb
Symbol name endothelin receptor type B (Rat)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse dopachrome tautomerase promoter
4 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 14
Common name neo; neomycin;
Symbol description No Data
References Cell. 1994 Dec 30;79(7):1267-76.


Targeted and natural (piebald-lethal) mutations of endothelin-B receptor gene produce megacolon associated with spotted coat color in mice.
Targeted and natural (piebald-lethal) mutations of endothelin-B receptor gene produce megacolon associated with spotted coat color in mice.
Targeted and natural (piebald-lethal) mutations of endothelin-B receptor gene produce megacolon associated with spotted coat color in mice.
Targeted and natural (piebald-lethal) mutations of endothelin-B receptor gene produce megacolon associated with spotted coat color in mice.
Research applications Fluorescent Proteins/lacZ System,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
The DEPOSITOR of BIOLOGICAL RESOURCE must obtain a consent on use of it from the DEVELOPER of the Ednrbtm1Ywa and Tg(DBH-Ednrb)Ywa (Professor Masashi Yanagisawa, University of Tsukuba (yanagisawa.masa.fu@u.tsukuba.ac.jp) and the DEVELOPER of Tg(Dct-lacZ)A12Jkn (Professor Ian J.Jackson, Western General Hospital(Edinburgh, UK)(ian.jackson@igmm.ed.ac.uk) before providing the mice to the RECIPIENT.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Kato, Masashi (Chubu University College of Life and Health Sciences) Kato, Masashi
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC05939, B6.Cg-Tg(Mt1-RET)304Ina Hr<hr>

RBRC No. RBRC05939
Type Transgene and Spontaneous MutationCartagena
Species Mus musculus
Strain name B6.Cg-Tg(Mt1-RET)304Ina Hr<hr>
Former Common name HL-RET-mice (304line)
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Masashi Kato, Nagoya University Graduate School of Medicine. Generated by crossing of RET mice and Hr mice (Hos:HRM). The mice were backcrossed to C57BL/6 over 10 times.
Strain description Mt1-Ret 304 mice with hairless mutant. The mice express the human RET under the control of the mouse MT1 promoter/enhancer. This strain is a model of cutaneous melanosis and develops tumors on the skin. Tg homozygous mice are embryonic lethal.
Colony maintenance Tg: Carrier x Noncarrier; hr: Homozygote x Homozygote
Health Report
Gene Details
Promoter No Data
1 Symbol Hr
Symbol name hairless
Chromosome 14
Common name No Data
Symbol description No Data
2 Symbol polyA
Symbol name SV40 splicing signal-polyadenylation site
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse metallothionein-I promoter-enhancer
3 Symbol RET
Symbol name ret proto-oncogene (human)
Chromosome UN
Common name No Data
Symbol description No Data
References Oncogene. 1998 Oct 8;17(14):1885-8.

Transgenic mouse model for skin malignant melanoma.
Transgenic mouse model for skin malignant melanoma.
Research applications Cancer Research,
Dermatology Research,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Kato, Masashi (Chubu University College of Life and Health Sciences) Kato, Masashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC05943, B6.Cg-Tg(Mt1-RET)242Ina Tg(Dct-LacZ)A12Jkn

RBRC No. RBRC05943
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(Mt1-RET)242Ina Tg(Dct-LacZ)A12Jkn
Former Common name 242RET;Dct-LacZ
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Masashi Kato, Nagoya University Graduate School of Medicine. Generated by crossing of RET mice and Hr mice (Hos:HRM). The mice were backcrossed to C57BL/6 over 10 times.
Strain description Mt1-RET/Dct-lacZ Tg mice. These mice express the lacZ in melanocytes under the control of Dct promoter and the human RET under the control of the mouse MT1 promoter/enhancer. Mt1-RET homozygous mutant mice are embryonic lethal.
Colony maintenance Mt1-RET: Carrier x Noncarrier; Dct-lacZ: Carrier x Carrier (Homozygote x Homozygote)
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 splicing signal-polyadenylation site, vector sequence
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Dct promoter
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse metallothionein-I promoter-enhancer
3 Symbol RET
Symbol name ret proto-oncogene (human)
Chromosome UN
Common name No Data
Symbol description No Data
References EMBO J. 1991 Nov;10(11):3167-75.

Aberrant melanogenesis and melanocytic tumour development in transgenic mice that carry a metallothionein/ret fusion gene.
Aberrant melanogenesis and melanocytic tumour development in transgenic mice that carry a metallothionein/ret fusion gene.
Research applications Cancer Research,
Dermatology Research,
Fluorescent Proteins/lacZ System,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Kato, Masashi (Chubu University College of Life and Health Sciences) Kato, Masashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC05944, B6.Cg-Tg(Mt1-RET)304Ina Tg(Dct-LacZ)A12Jkn

RBRC No. RBRC05944
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(Mt1-RET)304Ina Tg(Dct-LacZ)A12Jkn
Former Common name 304RET;Dct-LacZ
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Masashi Kato, Nagoya University Graduate School of Medicine. C57BL/6 background. The Dct-lacZ Tg allele was generated by Ian Jackson, Western General Hospital.
Strain description Mt1-RET/Dct-lacZ Tg mice. These mice express the lacZ in melanocytes under the control of Dct promoter and the human RET under the control of the mouse MT1 promoter/enhancer. Mt1-RET homozygous mutant mice are embryonic lethal.
Colony maintenance Mt1-RET: Carrier x Noncarrier; Dct-lacZ: Carrier x Carrier (Homozygote x Homozygote)
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 splicing signal-polyadenylation site
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter mouse Dct promoter
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse metallothionein-I promoter-enhancer
3 Symbol RET
Symbol name ret proto-oncogene (human)
Chromosome UN
Common name No Data
Symbol description No Data
References Oncogene. 1998 Oct 8;17(14):1885-8.

Transgenic mouse model for skin malignant melanoma.
Transgenic mouse model for skin malignant melanoma.
Research applications Cancer Research,
Dermatology Research,
Fluorescent Proteins/lacZ System,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Kato, Masashi (Chubu University College of Life and Health Sciences) Kato, Masashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC05975, B6.Cg-Tg(Mt1-RET)242Ina Hr<hr>

RBRC No. RBRC05975
Type Transgene and Spontaneous MutationCartagena
Species Mus musculus
Strain name B6.Cg-Tg(Mt1-RET)242Ina Hr<hr>
Former Common name HL-RET-mice (242line)
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Masashi Kato, Nagoya University Graduate School of Medicine. Generated by crossing of RET mice and Hr mice (Hos:HRM). The mice were backcrossed to C57BL/6 over 10 times.
Strain description Mt1-Ret 242 mice with hairless mutant. The mice express the human RET under the control of the mouse MT1 promoter/enhancer. Mt1-RET homozygous mutant mice are embryonic lethal.
Colony maintenance Tg: Carrier x Noncarrier; hr: Homozygote x Homozygote
Health Report
Gene Details
Promoter No Data
1 Symbol Hr
Symbol name hairless
Chromosome 14
Common name No Data
Symbol description No Data
2 Symbol polyA
Symbol name SV40 splicing signal-polyadenylation site
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Metallothionein-I promoter-enhancer
3 Symbol RET
Symbol name ret proto-oncogene (human)
Chromosome UN
Common name No Data
Symbol description No Data
References EMBO J. 1991 Nov;10(11):3167-75.
J Invest Dermatol. 2006 Dec 7;.

Aberrant melanogenesis and melanocytic tumour development in transgenic mice that carry a metallothionein/ret fusion gene.
Aberrant melanogenesis and melanocytic tumour development in transgenic mice that carry a metallothionein/ret fusion gene.
Research applications Cancer Research,
Dermatology Research,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Kato, Masashi (Chubu University College of Life and Health Sciences) Kato, Masashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06249, B6.129-Ret<tm1Mat>

RBRC No. RBRC06249
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129-Ret<tm1Mat>
Former Common name B6-Ret YF KI, B6-Ret/YF KI
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Masahide Takahashi, Nagoya University Graduate School of Medicine. ES cells derived from 129 were used for generation of the mice. The mutant mice were backcrossed to C57BL/6 over 16 times.
Strain description Ret Y1062F variant knock-in mice having two amino acid replacement (KLY<1062> to KIF<1062>) within exon 19 and a floxed PGK-neo cassette inserted into intron 19. Homozygous mutant mice are born normally, but exhibit megacolon and deafness and die by day 27 after birth.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJmsSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol Ret
Symbol name ret proto-oncogene
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Mouse Models for Human Disease,
Sensorineural Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Kato, Masashi (Chubu University College of Life and Health Sciences) Kato, Masashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC06250, ICR.Cg-Tg(Mt1-RET)242Ina

RBRC No. RBRC06250
Type TransgeneCartagena
Species Mus musculus
Strain name ICR.Cg-Tg(Mt1-RET)242Ina
Former Common name ICR-RET#242, ICR-Mt1-RET Tg#242
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Masashi Kato, Nagoya University Graduate School of Medicine. The Mt1-RET transgene was injected into the fertilized eggs of (BALB/c x C57BL/6) x BALB/c mice. ICR mixed background.
Strain description Mt1-Ret 242 transgenic mice. The mice express the human RET under the control of the mouse MT1 promoter/enhancer. Mt1-RET homozygous mutant mice are embryonic lethal.
Colony maintenance Carrier x Noncarrier [or Crossing to Slc:ICR]
Health Report
Gene Details
Promoter No Data
1 Symbol polyA
Symbol name SV40 splicing signal-polyadenylation site
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Metallothionein-I promoter-enhancer
2 Symbol RET
Symbol name ret proto-oncogene (human)
Chromosome UN
Common name No Data
Symbol description No Data
References EMBO J. 1991 Nov;10(11):3167-75.
Oncogene. 1999 Jan 21;18(3):837-42.

Aberrant melanogenesis and melanocytic tumour development in transgenic mice that carry a metallothionein/ret fusion gene.
Aberrant melanogenesis and melanocytic tumour development in transgenic mice that carry a metallothionein/ret fusion gene.
Research applications Cancer Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Kato, Masashi (Chubu University College of Life and Health Sciences) Kato, Masashi
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06251, B6.Cg-Ret<tm1Mat> Ptch1<tm1Mps>

RBRC No. RBRC06251
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Ret<tm1Mat> Ptch1<tm1Mps>
Former Common name Ret YF; Ptch1 KO, B6-RetYF KI/Ptch1 KO
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Ret Y1062F knock-in: Developed by Masahide Takahashi, Nagoya University Graduate School of Medicine. Ptch1 KO: Developed by Matthew Scott, Stanford University School of Medicine.
Strain description Ret Y1062F knock-in and Ptch1 KO double mutant mice. The Ret Y1062F knock-in allele consists of two amino acid replacement (KLY<1062> to KIF<1062>) within exon 19 and a floxed PGK-neo cassette inserted into intron 19. Part of exon 1 and all of exon 2 of Ptch1 gene were replaced with a lacZ-neo cassette in the Ptch1 KO allele.
Colony maintenance Ret: Heterozygote x Wild-type; Ptch1: Heterozygote x Wild-type [or Crossing to C57BL/6JJmsSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol Ptch1
Symbol name patched homolog 1
Chromosome 13
Common name No Data
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 13
Common name No Data
Symbol description No Data
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 13
Common name No Data
Symbol description No Data
4 Symbol Ret
Symbol name ret proto-oncogene
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
6 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Kato, Masashi (Chubu University College of Life and Health Sciences) Kato, Masashi
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :