RBRC05502, 129-Cfm2<tm1Tfur>

RBRC No. RBRC05502
Type Targeted MutationCartagena
Species Mus musculus
Strain name 129-Cfm2<tm1Tfur>
Former Common name 129-Cfm2 KO
H-2 Haplotype No Data
ES cell line TC1 [129S6/SvEvTac]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takahisa Furukawa, Osaka Bioscience Institute in 2007. TC1/TC-1 ES cells were used. 129 and C57BL/6 mixed background.
Strain description Cfm2 gene knockout mice. Exon 1 of Cfm2 gene was replaced with PGK-neo cassette. Homozygous mutant mice show no obvious abnormality.
Colony maintenance Homozygote x Homozygote, Heterozygote [or Crossing to C57BL/6NCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol Fam101a
Symbol name family with sequence similarity 101, member A
Chromosome 5
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name SV40 Poly A signal
Chromosome 5
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 5
Common name neo; neomycin;
Symbol description No Data
References Unpublished

Research applications Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
未定(後日指定)
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Furukawa, Takahisa (Osaka Bioscience Institute) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06573, B6.Cg-Tg(Dkk3-EGFP)1Tfur

RBRC No. RBRC06573
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(Dkk3-EGFP)1Tfur
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takahisa Furukawa, Osaka Bioscience Institute in 2008. The BAC-Dkk3-EGFP transgene was injected into the pronuclei of B6C3F1 fertilized eggs.
Strain description BAC-Dkk3-EGFP transgenic mice. This transgenic mouse expresses EGFP under the control of the mouse Dkk3 promoter. 129 background (RBRC05561), C57BL/6 mixed background (RBRC06573).
Colony maintenance Carrier x Noncarrier [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References Unpublished

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
後日指定
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor Furukawa, Takahisa (Osaka Bioscience Institute) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09682, C57BL/6-A930003A15Rik<tm1Tfur>

RBRC No. RBRC09682
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6-A930003A15Rik<tm1Tfur>
Former Common name D15 KO mouse
H-2 Haplotype No Data
ES cell line JM8A3 [C57BL/6N-A]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takahisa Furukawa, Institute for Protein Research, Osaka University in 2015. JM8A3 (C57BL/6N) ES cell line was used. Mice were maintained in the C57BL/6 background.
Strain description A930003A15Rik knockout mice. A930003A15Rik is a long noncoding RNA and highly expressed at the inner retina.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome 16
Common name No Data
Symbol description No Data
2 Symbol A930003A15Rik
Symbol name RIKEN cDNA A930003A15 gene
Chromosome 16
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 16
Common name No Data
Symbol description No Data
4 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 16
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 16
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
6 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 16
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must perform the study on a collaboration basis with the DEPOSITOR.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Furukawa, Takahisa (Institute for Protein Research, Osaka University) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC10907, C57BL/6-Smim40<tm1Tfur>

RBRC No. RBRC10907
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6-Smim40<tm1Tfur>
Former Common name B6- D18-Het
H-2 Haplotype No Data
ES cell line JM8A3 [C57BL/6N-A]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome 17
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 17
Common name No Data
Symbol description No Data
3 Symbol Smim40
Symbol name small integral membrane protein 40
Chromosome 17
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 17
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
5 Symbol neo
Symbol name No Data
Chromosome 17
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT which wants to use the BIOLOGICAL RESOURCE is requested to contact the depositor (Dr. Takahisa Furukawa) and enter into a Material Transfer Agreement with Osaka University.
Additional information
1 No Data
Depositor Furukawa, Takahisa (Institute for Protein Research, Osaka University) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05509, B6.129S6-Trpm1<tm1Tfur>

RBRC No. RBRC05509
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129S6-Trpm1<tm1Tfur>
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takahisa Furukawa, Osaka Bioscience Institute in 2005. A neomycin selection cassette was inserted into the exon 4-6 of the TRPM1 gene. C57BL/6 background. 129 background (RBRC05138), B6 background (RBRC05509).
Strain description Trpm1 knockout mice. A neomycin selection cassette was inserted into the exon 4-6 of the TRPM1 gene.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJmsSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol Trpm1
Symbol name transient receptor potential cation channel, subfamily M, member 1
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name SV40 Poly A signal
Chromosome 7
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 7
Common name neo; neomycin;
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
Additional information
1 No Data
Depositor Furukawa, Takahisa (Osaka Bioscience Institute) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06574, B6.Cg-Tg(Chx10-cre/ERT2)G7Tfur

RBRC No. RBRC06574
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(Chx10-cre/ERT2)G7Tfur
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takahisa Furukawa, Osaka Bioscience Institute in 2010. The BAC-Chx10-creERT2 transgene was injected into the pronuclei of B6C3F1 fertilized eggs.
Strain description BAC-Chx10-creERT2 transgenic mice. This transgenic mouse expresses tamoxifen-inducible cre recombinase under the control of the mouse Chx10 promoter. 129 background (RBRC05565), C57BL/6 mixed background (RBRC06574).
Colony maintenance Carrier x Carrier, Noncarrier [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 polyA signal
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol ERT2
Symbol name estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Vsx2 promoter
3 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References Unpublished

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
後日指定
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Furukawa, Takahisa (Osaka Bioscience Institute) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09689, B6(Cg)-Tmem132a<tm1c(KOMP)Wtsi>/Tfur

RBRC No. RBRC09689
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6(Cg)-Tmem132a<tm1c(KOMP)Wtsi>/Tfur
Former Common name Tmem132a flox
H-2 Haplotype No Data
ES cell line JM8A3.N1 [C57BL/6N-A]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takahisa Furukawa, Institute for Protein Research, Osaka University. JM8A3 (C57BL/6N) ES cell line was used. Mice were maintained in a mixed background of C57BL/6J and C3H. A IRES-LacZ/Neo cassette was removed by a FLP mouse line.
Strain description Floxed mice of Tmem132a. Exon 2 and 3 were flanked by loxP sites.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 19
Common name No Data
Symbol description No Data
2 Symbol Tmem132a
Symbol name transmembrane protein 132A
Chromosome 19
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 19
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE,
an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested. The availability of the BIOLOGICALRESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must perform the study on a collaboration basis with the DEPOSITOR. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
Additional information
1 No Data
Depositor Furukawa, Takahisa (Institute for Protein Research, Osaka University) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05552, 129S6.129S(Cg)-Grm6<tm1Nak>

RBRC No. RBRC05552
Type Targeted MutationCartagena
Species Mus musculus
Strain name 129S6.129S(Cg)-Grm6<tm1Nak>
Former Common name 129-mGluR6 KO, 129-Grm6 KO
H-2 Haplotype No Data
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]
Background strain 129S6/SvEvTac
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Shigetada Nakanishi and Takahisa Furukawa, Osaka Bioscience Institute in 2012. The mutant mice were generated by backcrossing to 129S6/SvEvTac from B6-Grm6-lacZ Tg/Grm6 KO mice (RBRC01353).
Strain description mGluR6 (Grm6) knockout mice. 126 background. 1.2 kb sequence encoding part of the transmembrane region of Grm6 gene was replaced with a neomycin resistance cassette.
Colony maintenance Heterozygote x Wild-type [or Crossing to 129S6/SvEvTac]
Health Report
Gene Details
Promoter No Data
1 Symbol Grm6
Symbol name glutamate receptor, metabotropic 6; targeted mutation 1, Shigetada Nakanishi
Chromosome 11
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References Cell. 1995 Mar 10;80(5):757-65.
J Neurosci. 1999 Apr 1;19(7):2568-79.

Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene.
Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene.
Research applications Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Cell, 80, 757-765 (1995). J. Neurosci., 19, 2568-2579 (1999).
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Furukawa, Takahisa (Osaka Bioscience Institute) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
Transsynaptic Binding of Orphan Receptor GPR179 to Dystroglycan-Pikachurin Complex Is Essential for the Synaptic Organization of Photoreceptors. Cell Rep.25(1): 130-145 (2018).(30282023)
Orlandi C, Omori Y, Wang Y, Cao Y, Ueno A, Roux MJ, Condomitti G, de Wit J, Kanagawa M, Furukawa T, Martemyanov KA.

RBRC09366, C3;B6-Mef2d<tm1a-flox-tfur>

RBRC No. RBRC09366
Type Targeted MutationCartagena
Species Mus musculus
Strain name C3;B6-Mef2d<tm1a-flox-tfur>
Former Common name Mef2d flox; C3;B6-Mef2d
H-2 Haplotype No Data
ES cell line TC1 [129S6/SvEvTac]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 3
Common name No Data
Symbol description No Data
2 Symbol Mef2d
Symbol name myocyte enhancer factor 2D
Chromosome 3
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 3
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
Developmental Biology Research,
FLP/frt system,
Neurobiology Research,
Sensorineural Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Furukawa, Takahisa (Institute for Protein Research, Osaka University) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09714, B6(C3)-Vsx2<tm1.1(cre/ERT2)Tfur>

RBRC No. RBRC09714
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6(C3)-Vsx2<tm1.1(cre/ERT2)Tfur>
Former Common name Chx10-IRES-CreERT2 KI
H-2 Haplotype No Data
ES cell line JM8A3 [C57BL/6N-A]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takahisa Furukawa, Institute for Protein Research, Osaka University. JM8A3 (C57BL/6N) ES cell line was used. This knock-in mice were mated to mice in B6.C3H background and then mated to mice in C57BL/6J background.
Strain description IRES-CreERT2 knock-in mice. An IRES-CreERT2 cassette was placed just downstream of stop codon of the Vsx2 (visual system homeobox 2, Chx10) gene. A Neo gene cassette was removed by a CAG-Cre mouse line. IRES-CreERT2 can be removed by a Flp recombinase.
Colony maintenance Heterozygote x Wild-type [or Crossing to C57BL/6JJmsSlc]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol ERT2
Symbol name estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)
Chromosome 12
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 12
Common name No Data
Symbol description No Data
3 Symbol Vsx2
Symbol name visual system homeobox 2
Chromosome 12
Common name No Data
Symbol description No Data
4 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome 12
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 12
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must perform the study on a collaboration basis with the DEPOSITOR.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Furukawa, Takahisa (Institute for Protein Research, Osaka University) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months