RBRC09366, C3;B6-Mef2d<tm1a-flox-tfur>

RBRC No. RBRC09366
Type Targeted MutationCartagena
Species Mus musculus
Strain name C3;B6-Mef2d<tm1a-flox-tfur>
Former Common name Mef2d flox; C3;B6-Mef2d
H-2 Haplotype No Data
ES cell line TC1 [129S6/SvEvTac]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 3
Common name No Data
Symbol description No Data
2 Symbol Mef2d
Symbol name myocyte enhancer factor 2D
Chromosome 3
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 3
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
Developmental Biology Research,
FLP/frt system,
Neurobiology Research,
Sensorineural Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Furukawa, Takahisa (Institute for Protein Research, Osaka University) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09714, B6(C3)-Vsx2<tm1.1(cre/ERT2)Tfur>

RBRC No. RBRC09714
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6(C3)-Vsx2<tm1.1(cre/ERT2)Tfur>
Former Common name Chx10-IRES-CreERT2 KI
H-2 Haplotype No Data
ES cell line JM8A3 [C57BL/6N-A]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takahisa Furukawa, Institute for Protein Research, Osaka University. JM8A3 (C57BL/6N) ES cell line was used. This knock-in mice were mated to mice in B6.C3H background and then mated to mice in C57BL/6J background.
Strain description IRES-CreERT2 knock-in mice. An IRES-CreERT2 cassette was placed just downstream of stop codon of the Vsx2 (visual system homeobox 2, Chx10) gene. A Neo gene cassette was removed by a CAG-Cre mouse line. IRES-CreERT2 can be removed by a Flp recombinase.
Colony maintenance Heterozygote x Wild-type [or Crossing to C57BL/6JJmsSlc]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol ERT2
Symbol name estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)
Chromosome 12
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 12
Common name No Data
Symbol description No Data
3 Symbol Vsx2
Symbol name visual system homeobox 2
Chromosome 12
Common name No Data
Symbol description No Data
4 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome 12
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 12
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must perform the study on a collaboration basis with the DEPOSITOR.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Furukawa, Takahisa (Institute for Protein Research, Osaka University) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05552, 129S6.129S(Cg)-Grm6<tm1Nak>

RBRC No. RBRC05552
Type Targeted MutationCartagena
Species Mus musculus
Strain name 129S6.129S(Cg)-Grm6<tm1Nak>
Former Common name 129-mGluR6 KO, 129-Grm6 KO
H-2 Haplotype No Data
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]
Background strain 129S6/SvEvTac
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Shigetada Nakanishi and Takahisa Furukawa, Osaka Bioscience Institute in 2012. The mutant mice were generated by backcrossing to 129S6/SvEvTac from B6-Grm6-lacZ Tg/Grm6 KO mice (RBRC01353).
Strain description mGluR6 (Grm6) knockout mice. 126 background. 1.2 kb sequence encoding part of the transmembrane region of Grm6 gene was replaced with a neomycin resistance cassette.
Colony maintenance Heterozygote x Wild-type [or Crossing to 129S6/SvEvTac]
Health Report
Gene Details
Promoter No Data
1 Symbol Grm6
Symbol name glutamate receptor, metabotropic 6; targeted mutation 1, Shigetada Nakanishi
Chromosome 11
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References Cell. 1995 Mar 10;80(5):757-65.
J Neurosci. 1999 Apr 1;19(7):2568-79.

Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene.
Specific deficit of the ON response in visual transmission by targeted disruption of the mGluR6 gene.
Research applications Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Cell, 80, 757-765 (1995). J. Neurosci., 19, 2568-2579 (1999).
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Furukawa, Takahisa (Osaka Bioscience Institute) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
Transsynaptic Binding of Orphan Receptor GPR179 to Dystroglycan-Pikachurin Complex Is Essential for the Synaptic Organization of Photoreceptors. Cell Rep.25(1): 130-145 (2018).(30282023)
Orlandi C, Omori Y, Wang Y, Cao Y, Ueno A, Roux MJ, Condomitti G, de Wit J, Kanagawa M, Furukawa T, Martemyanov KA.

RBRC09367, C3;B6-Mef2d<tm1.2Tfur>

RBRC No. RBRC09367
Type Targeted MutationCartagena
Species Mus musculus
Strain name C3;B6-Mef2d<tm1.2Tfur>
Former Common name Mef2d KO
H-2 Haplotype No Data
ES cell line TC1 [129S6/SvEvTac]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 3
Common name No Data
Symbol description No Data
2 Symbol Mef2d
Symbol name myocyte enhancer factor 2D
Chromosome 3
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 3
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
Developmental Biology Research,
FLP/frt system,
Neurobiology Research,
Sensorineural Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Furukawa, Takahisa (Institute for Protein Research, Osaka University) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09715, B6;B6C3-Tg(Grm6-FLPo)1Tfur

RBRC No. RBRC09715
Type TransgeneCartagena
Species Mus musculus
Strain name B6;B6C3-Tg(Grm6-FLPo)1Tfur
Former Common name mGluR6-FLPo
H-2 Haplotype No Data
Background strain C57BL/6JJmsSlc
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takahisa Furukawa, Institute for Protein Research, Osaka University. A DNA construct was injected to fertilized eggs derived from B6C3HF1. The mice were mated to mice in C57BL/6J background.
Strain description FLPo Transgenic mice under the control of Grm6 (mGluR6) gene promoter.
Colony maintenance Heterozygote x Wild-type [or Crossing to C57BL/6JJmsSlc]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 polyA
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Grm6 promoter
2 Symbol FLPo
Symbol name yeast flippase recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University). The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must perform the study on a collaboration basis with the DEPOSITOR.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Furukawa, Takahisa (Institute for Protein Research, Osaka University) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05558, 129(Cg)-Rax<tm1.1Tfur>

RBRC No. RBRC05558
Type Targeted MutationCartagena
Species Mus musculus
Strain name 129(Cg)-Rax<tm1.1Tfur>
Former Common name 129-Rax floxed
H-2 Haplotype No Data
ES cell line TC1 [129S6/SvEvTac]
Background strain 129S6/SvEvTac
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takahisa Furukawa, Osaka Bioscience Institute in 2006. 129 background. A Frt-flanked PGK-neo selection cassette was removed by crossing with FLP1 mice (B6;SJL-Tg(ACTFLPe)9205Dym/J).
Strain description Rax gene floxed mice. Exons 1 and 2 of the Rax gene were flanked by loxP sites. Conditional Rax deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenance Homozygote x Homozygote [or Crossing to 126S6/SvEvTac]
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 18
Common name No Data
Symbol description No Data
2 Symbol Rax
Symbol name retina and anterior neural fold homeobox; targeted mutation 1.1, Takahisa Furukawa
Chromosome 18
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 18
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neurosci., 31, 16792-16807 (2011).
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Furukawa, Takahisa (Osaka Bioscience Institute) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months