RBRC10594, B6;B6D2-Tg(Piwil2-Flag/NLS/AZf/Piwil4)#6Nkn

RBRC No. RBRC10594
Type TransgeneCartagena
Species Mus musculus
Strain name B6;B6D2-Tg(Piwil2-Flag/NLS/AZf/Piwil4)#6Nkn
Former Common name Tg-MiliP-FNAZf-Miwi2
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name No Data
Chromosome No Data
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Kojima-Kita K, Kuramochi-Miyagawa S, Nagamori I, Ogonuki N, Ogura A, Hasuwa H, Akazawa T, Inoue N, Nakano T. MJWI2 as an Effector of DNA Methylation and Gene Silencing in Embryonic Male Germ Cells. Cell Rep. 2016 Sep 13;16(11):2819-2828. do1: 10.1016/j.celrep.2016.08.027. PMID: 27626653
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education
or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 No Data
Depositor Nakano, Toru (Osaka university) Nakano, Toru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC10595, B6;B6D2-Tg(Piwil2-Flag/NLS/AZf/Piwil4)#7Nkn

RBRC No. RBRC10595
Type TransgeneCartagena
Species Mus musculus
Strain name B6;B6D2-Tg(Piwil2-Flag/NLS/AZf/Piwil4)#7Nkn
Former Common name Tg-MiliP-FNAZf-M1wi2
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name No Data
Chromosome No Data
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Kojima-Kita K, Kuramochi-Miyagawa S, Nagamori I, Ogonuki N, Ogura A, Hasuwa H, Akazawa T, Inoue N, Nakano T. MJWI2 as an Effector of DNA Methylation and Gene Silencing in Embryonic Male Germ Cells. Cell Rep. 2016 Sep 13;16(11):2819-2828. do1: 10.1016/j.celrep.2016.08.027. PMID: 27626653
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education
or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 No Data
Depositor Nakano, Toru (Osaka university) Nakano, Toru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02109, B6;129-Gmcl1<tm1Nak>

RBRC No. RBRC02109
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6;129-Gmcl1<tm1Nak>
Former Common name mouse germ cell-less homologue-1 KO
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Gmcl1
Symbol name germ cell-less homolog 1 (Drosophila)
Chromosome 6
Common name 2810049L19Rik, Gcl, mglc-1
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name neo; neomycin;
Symbol description No Data
References Mol Cell Biol. 2003 Feb;23(4):1304-15.

Mouse germ cell-less as an essential component for nuclear integrity.
Research applications Reproductive Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol. Cell. Biol., 23, 1304-1315 ( 2003).
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Nakano, Toru (Osaka university) Nakano, Toru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09475, B6.129S2-Piwil2<tm1Nkn>

RBRC No. RBRC09475
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129S2-Piwil2<tm1Nkn>
Former Common name Mili-KO
H-2 Haplotype No Data
ES cell line D3 [129S2/SvPas]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Piwil2
Symbol name piwi-like RNA-mediated gene silencing 2
Chromosome 14
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 14
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Development., 131, 839-849 (2004).
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (by e-mail).
Additional information
1 No Data
Depositor Nakano, Toru (Osaka university) Nakano, Toru
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09476, B6.129S2-Piwil4<tm1Nkn>

RBRC No. RBRC09476
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129S2-Piwil4<tm1Nkn>
Former Common name Miwi2-KO
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Piwil4
Symbol name piwi-like RNA-mediated gene silencing 4
Chromosome 9
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 9
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genes Dev., 1, 908-917 (2008).
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (by e-mail).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Nakano, Toru (Osaka university) Nakano, Toru
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC10587, C57BL/6-Pnldc1<em1Nkn>

RBRC No. RBRC10587
Type CRISPR/Cas9
Species Mus musculus
Strain name C57BL/6-Pnldc1<em1Nkn>
Former Common name Pnldcl_del-1lnt-Exon3, Pnldc1-KO
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Cas9 mRNA and each single-guide RNA (sgRNA) transcribed in vitro by the mMESSAGE mMACHINE T7 Ultra Kit (Ambion) were injected into C57BL6 zygotes. Two-cell-stage embryos were transplanted into pseudopregnant mice.
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Pnldc1
Symbol name poly(A)-specific ribonuclease (PARN)-like domain containing 1
Chromosome 17
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nishimura T, Nagamori I, Nakatani T, Izumi N, Tomari Y, Kuramochi-Miyagawa S, Nakano T. PNLDCl, mouse pre-piRNA Trimmer, is required for meiotic and post-meiotic male germ cell development. EMBO Rep. 2018 Mar;l9(3). pii: e44957. doi: 10.15252/embr.201744957.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 No Data
Depositor Nakano, Toru (Osaka university) Nakano, Toru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC10588, C57BL/6-Pnldc1<em2Nkn>

RBRC No. RBRC10588
Type CRISPR/Cas9
Species Mus musculus
Strain name C57BL/6-Pnldc1<em2Nkn>
Former Common name Pnldcl_Exon 7-mut, Pnldc1-mut
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Cas9 mRNA and each single-guide RNA (sgRNA) transcribed in vitro by the mMESSAGE mMACHINE T7 Ultra Kit (Ambion) were injected into C57BL6 zygotes. Two-cell-stage embryos were transplanted into pseudopregnant mice.
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Pnldc1
Symbol name poly(A)-specific ribonuclease (PARN)-like domain containing 1
Chromosome 17
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nishimura T, Nagamori I, Nakatani T, Izumi N, Tomari Y, Kuramochi-Miyagawa S, Nakano T. PNLDCl, mouse pre-piRNA Trimmer, is required for meiotic and post-meiotic male germ cell development. EMBO Rep. 2018 Mar;l9(3). pii: e44957. doi: 10.15252/embr.201744957.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education
or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 No Data
Depositor Nakano, Toru (Osaka university) Nakano, Toru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC10589, C57BL/6-Pnldc1<em3Nkn>

RBRC No. RBRC10589
Type CRISPR/Cas9
Species Mus musculus
Strain name C57BL/6-Pnldc1<em3Nkn>
Former Common name Pnldcl_del-14nt-Exon3, Pnldc1-KO
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Cas9 mRNA and each single-guide RNA (sgRNA) transcribed in vitro by the mMESSAGE mMACHINE T7 Ultra Kit (Ambion) were injected into C57BL6 zygotes. Two-cell-stage embryos were transplanted into pseudopregnant mice.
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Pnldc1
Symbol name poly(A)-specific ribonuclease (PARN)-like domain containing 1
Chromosome 17
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nishimura T, Nagamori I, Nakatani T, Izumi N, Tomari Y, Kuramochi-Miyagawa S, Nakano T. PNLDCl, mouse pre-piRNA Trimmer, is required for meiotic and post-meiotic male germ cell development. EMBO Rep. 2018 Mar;l9(3). pii: e44957. doi: 10.15252/embr.201744957.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education
or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 No Data
Depositor Nakano, Toru (Osaka university) Nakano, Toru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC10590, B6;B6D2-Tg(Piwil2-Flag/NLS/Piwil4)#10Nkn

RBRC No. RBRC10590
Type TransgeneCartagena
Species Mus musculus
Strain name B6;B6D2-Tg(Piwil2-Flag/NLS/Piwil4)#10Nkn
Former Common name Tg-MiliP-FN-Miwi2
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name No Data
Chromosome No Data
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Kojima-Kita K, Kuramochi-Miyagawa S, Nagamori I, Ogonuki N, Ogura A, Hasuwa H, Akazawa T, Inoue N, Nakano T. MJWI2 as an Effector of DNA Methylation and Gene Silencing in Embryonic Male Germ Cells. Cell Rep. 2016 Sep 13;16(11):2819-2828. do1: 10.1016/j.celrep.2016.08.027. PMID: 27626653
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education
or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 No Data
Depositor Nakano, Toru (Osaka university) Nakano, Toru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC10591, B6;B6D2-Tg(Piwil2-Flag/NLS/Piwil4)#7Nkn

RBRC No. RBRC10591
Type TransgeneCartagena
Species Mus musculus
Strain name B6;B6D2-Tg(Piwil2-Flag/NLS/Piwil4)#7Nkn
Former Common name Tg-Mi!iP-FN-Miwi2
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name No Data
Chromosome No Data
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Kojima-Kita K, Kuramochi-Miyagawa S, Nagamori I, Ogonuki N, Ogura A, Hasuwa H, Akazawa T, Inoue N, Nakano T. MJWI2 as an Effector of DNA Methylation and Gene Silencing in Embryonic Male Germ Cells. Cell Rep. 2016 Sep 13;16(11):2819-2828. do1: 10.1016/j.celrep.2016.08.027. PMID: 27626653
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education
or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 No Data
Depositor Nakano, Toru (Osaka university) Nakano, Toru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months