RBRC05467, B6.Cg-Gt(ROSA)26Sor<tm4.1(CAG-mCitrine)Maoh>

RBRC No. RBRC05467
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Gt(ROSA)26Sor<tm4.1(CAG-mCitrine)Maoh>
Former Common name B6-ROSA26/CAG-mCitrine
H-2 Haplotype No Data
ES cell line E14TG2a [129P2/OlaHsd]carries Hprt
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Develop by Masato Ohtsuka, Tokai University School of Medicine in 2010. The mice were backcrossed to C57BL/6JJcl. Extra sequence in the RMCE<ex> was removed by FLP-mediated recombination.
Strain description The mice having a CAG-mCitrine-polyA cassette at the ROSA26 locus that were generated by RMCE (Recombinase-mediated cassette exchange). The mutant mice exhibit ubiquitous and very bright fluorescence.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name SV40 polyA
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol lox2272
Symbol name mutant lox sequence
Chromosome 6
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
5 Symbol mCitrine
Symbol name variant of Yellow Fluorescent Protein
Chromosome 6
Common name No Data
Symbol description No Data
References Nucleic Acids Res. 2010 Dec 1;38(22):e198. Epub 2010 Sep 29.

Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.
Research applications Cell Biology Research,
Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nucleic Acids Res., 38, e198 (2010). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Ohtsuka, Masato (Tokai University) Ohtsuka, Masato
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05468, B6.Cg-Gt(ROSA)26Sor<tm5.1(CAG-mOrange)Maoh>

RBRC No. RBRC05468
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Gt(ROSA)26Sor<tm5.1(CAG-mOrange)Maoh>
Former Common name B6-ROSA26/CAG-mOrange
H-2 Haplotype No Data
ES cell line E14TG2a [129P2/OlaHsd]carries Hprt
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Develop by Masato Ohtsuka, Tokai University School of Medicine in 2010. The mice were backcrossed to C57BL/6JJcl. Extra sequence in the RMCE<ex> was removed by FLP-mediated recombination.
Strain description The mice having a CAG-mOrange-polyA cassette at the ROSA26 locus that were generated by RMCE (Recombinase-mediated cassette exchange). The mutant mice exhibit ubiquitous and very bright fluorescence.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name SV40 polyA
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol lox2272
Symbol name mutant lox sequence
Chromosome 6
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
5 Symbol mOrange
Symbol name Red Fluorescent Protein (Discosoma sp.)
Chromosome 6
Common name No Data
Symbol description No Data
References Nucleic Acids Res. 2010 Dec 1;38(22):e198. Epub 2010 Sep 29.

Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.
Research applications Cell Biology Research,
Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nucleic Acids Res., 38, e198 (2010). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Ohtsuka, Masato (Tokai University) Ohtsuka, Masato
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05469, B6.Cg-H2-Tw3<tm2.1(CAG-EGFP)Maoh>

RBRC No. RBRC05469
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-H2-Tw3<tm2.1(CAG-EGFP)Maoh>
Former Common name B6-H2-Tw3/CAG-GFP
H-2 Haplotype No Data
ES cell line E14TG2a [129P2/OlaHsd]carries Hprt
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Develop by Masato Ohtsuka, Tokai University School of Medicine in 2010. The mice were backcrossed to C57BL/6JJcl. Extra sequence in the RMCE<ex> was removed by FLP-mediated recombination.
Strain description The mice having a CAG-EGFP-polyA cassette at the H2-T3 locus that were generated by RMCE (Recombinase-mediated cassette exchange). The mutant mice exhibit ubiquitous and very bright fluorescence.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol H2-T3
Symbol name histocompatibility 2, T region locus 3
Chromosome 17
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name SV40 polyA
Chromosome 17
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 17
Common name No Data
Symbol description No Data
4 Symbol lox2272
Symbol name mutant lox sequence
Chromosome 17
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
5 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome 17
Common name No Data
Symbol description No Data
References Nucleic Acids Res. 2010 Dec 1;38(22):e198. Epub 2010 Sep 29.

Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.
Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nucleic Acids Res., 38, e198 (2010). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Depositor Ohtsuka, Masato (Tokai University) Ohtsuka, Masato
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05470, C.Cg-Gt(ROSA)26Sor<tm3.1(CAG-mCFP)Maoh>

RBRC No. RBRC05470
Type Targeted MutationCartagena
Species Mus musculus
Strain name C.Cg-Gt(ROSA)26Sor<tm3.1(CAG-mCFP)Maoh>
Former Common name BALB-ROSA26/CAG-mCFP
H-2 Haplotype No Data
ES cell line E14TG2a [129P2/OlaHsd]carries Hprt
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Develop by Masato Ohtsuka, Tokai University School of Medicine in 2010. The mice were backcrossed to BALB/cAJcl. Extra sequence in the RMCE<ex> was removed by FLP-mediated recombination.
Strain description The mice having a CAG-mCFP-polyA cassette at the ROSA26 locus that were generated by RMCE (Recombinase-mediated cassette exchange). The mutant mice exhibit ubiquitous and very bright fluorescence.
Colony maintenance Homozygote x Homozygote [or Crossing to BALB/cAJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
References Nucleic Acids Res. 2010 Dec 1;38(22):e198. Epub 2010 Sep 29.

Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.
Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nucleic Acids Res., 38, e198 (2010). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 No Data
Depositor Ohtsuka, Masato (Tokai University) Ohtsuka, Masato
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05471, C.Cg-Gt(ROSA)26Sor<tm4.1(CAG-mCitrine)Maoh>

RBRC No. RBRC05471
Type Targeted MutationCartagena
Species Mus musculus
Strain name C.Cg-Gt(ROSA)26Sor<tm4.1(CAG-mCitrine)Maoh>
Former Common name BALB-ROSA26/CAG-mCitrine
H-2 Haplotype No Data
ES cell line E14TG2a [129P2/OlaHsd]carries Hprt
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Develop by Masato Ohtsuka, Tokai University School of Medicine in 2010. The mice were backcrossed to BALB/cAJcl. Extra sequence in the RMCE<ex> was removed by FLP-mediated recombination.
Strain description The mice having a CAG-mCitrine-polyA cassette at the ROSA26 locus that were generated by RMCE (Recombinase-mediated cassette exchange). The mutant mice exhibit ubiquitous and very bright fluorescence.
Colony maintenance Homozygote x Homozygote [or Crossing to BALB/cAJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name SV40 polyA
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol lox2272
Symbol name mutant lox sequence
Chromosome 6
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
5 Symbol mCitrine
Symbol name variant of Yellow Fluorescent Protein
Chromosome 6
Common name No Data
Symbol description No Data
References Nucleic Acids Res. 2010 Dec 1;38(22):e198. Epub 2010 Sep 29.

Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.
Research applications Cell Biology Research,
Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nucleic Acids Res., 38, e198 (2010). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 No Data
Depositor Ohtsuka, Masato (Tokai University) Ohtsuka, Masato
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05472, C.Cg-Gt(ROSA)26Sor<tm6.1(CAG-tdTomato)Maoh>

RBRC No. RBRC05472
Type Targeted MutationCartagena
Species Mus musculus
Strain name C.Cg-Gt(ROSA)26Sor<tm6.1(CAG-tdTomato)Maoh>
Former Common name BALB-ROSA26/CAG-tdTomato
H-2 Haplotype No Data
ES cell line E14TG2a [129P2/OlaHsd]carries Hprt
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Develop by Masato Ohtsuka, Tokai University School of Medicine in 2010. The mice were backcrossed to BALB/cAJcl. Extra sequence in the RMCE<ex> was removed by FLP-mediated recombination.
Strain description The mice having a CAG-tdTomato-polyA cassette at the ROSA26 locus that were generated by RMCE (Recombinase-mediated cassette exchange). The mutant mice exhibit ubiquitous and very bright fluorescence.
Colony maintenance Homozygote x Homozygote [or Crossing to BALB/cAJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name SV40 polyA
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol SA
Symbol name Splice acceptor
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol lox2272
Symbol name mutant lox sequence
Chromosome 6
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
6 Symbol tdTomato
Symbol name Red Fluorescent Protein (Discosoma sp.) tdTomato (tandem dimeric Tomato)
Chromosome 6
Common name No Data
Symbol description No Data
References Nucleic Acids Res. 2010 Dec 1;38(22):e198. Epub 2010 Sep 29.

Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.
Research applications Cell Biology Research,
Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nucleic Acids Res., 38, e198 (2010). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Ohtsuka, Masato (Tokai University) Ohtsuka, Masato
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC06517, C57BL/6-Gt(ROSA)26Sor<tm10(PITT)Maoh>

RBRC No. RBRC06517
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6-Gt(ROSA)26Sor<tm10(PITT)Maoh>
Former Common name TOKMO-3, B6-ROSA26/PITT KI
H-2 Haplotype No Data
ES cell line EGR-101 [C57BL/6N]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Masato Ohtsuka, Tokai University School of Medicine in 2014. EGR101 ES cells were used to generate the mutant mice.
Strain description SA-F14-attP-JT15-eGFP-T2A-neo-hOCT4 ZFN target site-pA-FRT-L-lox2272-F15 ROSA26 knock-in mice.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6N]
Health Report
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name Simian virus 40 (SV40) polyA
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol No Data
Symbol name Streptomyces lividans attachment site
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol No Data
Symbol name actinophage phi C31 attachment site
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol F14
Symbol name yeast FRT variant (F14)
Chromosome 6
Common name No Data
Symbol description No Data
7 Symbol F15
Symbol name yeast FRT variant (F15)
Chromosome 6
Common name No Data
Symbol description No Data
8 Symbol FRT-L
Symbol name yeast FRT variant (FRT-L)
Chromosome 6
Common name No Data
Symbol description No Data
9 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
10 Symbol JT15
Symbol name phage P1 loxP variant (JT15)
Chromosome 6
Common name No Data
Symbol description No Data
11 Symbol SA
Symbol name Splice acceptor (adenovirus)
Chromosome 6
Common name No Data
Symbol description No Data
12 Symbol T2A
Symbol name Thosea asigna virus 2A peptide (insect)
Chromosome 6
Common name No Data
Symbol description No Data
13 Symbol lox2272
Symbol name mutant lox sequence
Chromosome 6
Common name No Data
Symbol description No Data
14 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
後日指定。 (will be announced).
For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. The RECIPIENT agrees to include the DEPOSITOR/DEVELOPER as a co-author in the first publication of the results obtained from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Mouse of the Month Sep 2015
2 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Depositor Ohtsuka, Masato (Tokai University) Ohtsuka, Masato
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09661, B6.Cg-Gt(ROSA)26Sor<tm12.1(Nphs1-EGFP)Maoh>

RBRC No. RBRC09661
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Gt(ROSA)26Sor<tm12.1(Nphs1-EGFP)Maoh>
Former Common name Nephrin-EGFP mice
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name Simian virus 40 (SV40) polyA,
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol SA
Symbol name Splice acceptor
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol lox2272
Symbol name mutant lox sequence
Chromosome 6
Common name No Data
Symbol description No Data
Promoter mouse Nephrin promoter
6 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome 6
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
後日指定。 (will be announced).
For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor Ohtsuka, Masato (Tokai University) Ohtsuka, Masato
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05466, B6.129P2-Gt(ROSA)26Sor<tm2.1(CAG-EGFP)Maoh>

RBRC No. RBRC05466
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Gt(ROSA)26Sor<tm2.1(CAG-EGFP)Maoh>
Former Common name B6-ROSA26/CAG-GFP
H-2 Haplotype No Data
ES cell line E14TG2a [129P2/OlaHsd]carries Hprt
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Develop by Masato Ohtsuka, Tokai University School of Medicine in 2010. The mice were backcrossed to C57BL/6JJcl. Extra sequence in the RMCE<ex> was removed by FLPe injection.
Strain description The mice having a CAG-EGFP-polyA cassette at the ROSA26 locus that were generated by RMCE (Recombinase-mediated cassette exchange). The mutant mice exhibit ubiquitous and very bright fluorescence.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
References Nucleic Acids Res. 2010 Dec 1;38(22):e198. Epub 2010 Sep 29.

Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression.
Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nucleic Acids Res., 38, e198 (2010). For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor Ohtsuka, Masato (Tokai University) Ohtsuka, Masato
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months