RBRC10172, B6;Cg-Sftpc<tm1(cre/ERT2)Yasu>

RBRC No. RBRC10172
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6;Cg-Sftpc<tm1(cre/ERT2)Yasu>
Former Common name SftpC-CreERT2
H-2 Haplotype No Data
ES cell line V6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 pA
Chromosome 14
Common name No Data
Symbol description No Data
2 Symbol Bsd
Symbol name Aspergillus terreus blasticidin resistance gene (Bsd)
Chromosome 14
Common name No Data
Symbol description No Data
3 Symbol ERT2
Symbol name No Data
Chromosome 14
Common name No Data
Symbol description No Data
4 Symbol HBB2
Symbol name rabbit beta-globin poly A
Chromosome 14
Common name No Data
Symbol description No Data
5 Symbol Sftpc
Symbol name surfactant associated protein C
Chromosome 14
Common name No Data
Symbol description No Data
6 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome 14
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature Commun., 9, 2081 (2018).
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Yamada, Yasuhiro (Center for iPS Cell Research and Application, Kyoto University) Yamada, Yasuhiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC02415, C57BL/6-Tg(TRE-GFP)392Yasu

RBRC No. RBRC02415
Type TransgeneCartagena wks
Species Mus musculus
Strain name C57BL/6-Tg(TRE-GFP)392Yasu
Former Common name β-catenin/Tcf reporter mice
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Yasuhiro Yamada, Gifu University Graduate School of Medicine in 2007. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs. Line 392 (main line) (RBRC02415), line 622 (rbrc02416) and line 628 (RBRC02417).
Strain description Beta-catenin/Tcf reporter mice. These mice express GFP gene under the control of the Tcf/Lef binding sites and a minimal thymidine kinase promoter from the TOPFLASH plasmid. Immunohistochemistry with a GFP antibody can be used to assess the Tg expression in the intestine. In other organs except for the intestine. In the splenocytes, expression of GFP was observed by FACS analysis, In other organs, the GFP expression patterns are not examined yet.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 polyA
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter Tcf binding element, herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References Cancer Res. 2007 May 1;67(9):4079-87.
Carcinogenesis. 2008 Mar;29(3):666-72. Epub 2008 Jan 19.

Development of gastric tumors in Apc(Min/+) mice by the activation of the beta-catenin/Tcf signaling pathway.
Development of gastric tumors in Apc(Min/+) mice by the activation of the beta-catenin/Tcf signaling pathway.
Research applications Cancer Research,
Cell Biology Research,
Developmental Biology Research,
Fluorescent Proteins/lacZ System,
General Purpose,
Tet system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Depositor Yamada, Yasuhiro (Gifu University, Graduate School of Medicine) Yamada, Yasuhiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02416, C57BL/6-Tg(TRE-GFP)622Yasu

RBRC No. RBRC02416
Type TransgeneCartagena wks
Species Mus musculus
Strain name C57BL/6-Tg(TRE-GFP)622Yasu
Former Common name β-catenin/Tcf reporter mice
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter Tcf binding element, herpes simplex virus thymidine kinase promoter (HSV tk promoter)
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References Cancer Res. 2007 May 1;67(9):4079-87.
Carcinogenesis. 2008 Mar;29(3):666-72. Epub 2008 Jan 19.

Development of gastric tumors in Apc(Min/+) mice by the activation of the beta-catenin/Tcf signaling pathway.
Development of gastric tumors in Apc(Min/+) mice by the activation of the beta-catenin/Tcf signaling pathway.
Research applications Cancer Research,
Cell Biology Research,
Developmental Biology Research,
Fluorescent Proteins/lacZ System,
General Purpose,
Tet system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor Yamada, Yasuhiro (Gifu University, Graduate School of Medicine) Yamada, Yasuhiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02417, C57BL/6-Tg(TRE-GFP)628Yasu

RBRC No. RBRC02417
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(TRE-GFP)628Yasu
Former Common name β-catenin/Tcf reporter mice
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter Tcf binding element, herpes simplex virus thymidine kinase promoter (HSV tk promoter)
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References Cancer Res. 2007 May 1;67(9):4079-87.
Carcinogenesis. 2008 Mar;29(3):666-72. Epub 2008 Jan 19.

Development of gastric tumors in Apc(Min/+) mice by the activation of the beta-catenin/Tcf signaling pathway.
Development of gastric tumors in Apc(Min/+) mice by the activation of the beta-catenin/Tcf signaling pathway.
Research applications Cancer Research,
Cell Biology Research,
Developmental Biology Research,
Fluorescent Proteins/lacZ System,
General Purpose,
Tet system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor Yamada, Yasuhiro (Gifu University, Graduate School of Medicine) Yamada, Yasuhiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC03947, B6;129B6-Apc<tm1(GFP)Yasu>

RBRC No. RBRC03947
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129B6-Apc<tm1(GFP)Yasu>
Former Common name Apc-IRES-GFP
H-2 Haplotype No Data
ES cell line V6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Apc
Symbol name adenomatosis polyposis coli
Chromosome No Data
Common name No Data
Symbol description No Data
References No Data

Research applications Cancer Research,
Cell Biology Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor Yamada, Yasuhiro (Gifu University, Graduate School of Medicine) Yamada, Yasuhiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05543, B6;B6129-Rest<tm1.1Yasu>

RBRC No. RBRC05543
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;B6129-Rest<tm1.1Yasu>
Former Common name Rest cKO, B6-Rest floxed
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Yasuhiro Yamada, Gifu University Graduate School of Medicine / Center for iPS Cell Research and Application, Kyoto University. C57BL/6J and 129S4 mixed background. The neomycin resistant cassette was removed by Cre mediated recombination.
Strain description Rest, RE1-silencing transcription factor gene floxed mice. Exon 4 of Rest gene was replaced with a loxP-exon 4-IRES-GFP-loxP construct. Conditional Rest deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 5
Common name internal ribosomal entry site from EMCV
Symbol description No Data
2 Symbol Rest
Symbol name RE1-silencing transcription factor
Chromosome 5
Common name No Data
Symbol description No Data
3 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 5
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 5
Common name No Data
Symbol description No Data
References

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR, KYOTO UNIVERSITY at cira-keiyaku@cira.kyoto-u.ac.jp.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor Yamada, Yasuhiro (Center for iPS Cell Research and Application, Kyoto University) Yamada, Yasuhiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC10170, B6;Cg-Pdx1<tm1(cre)Yasu>

RBRC No. RBRC10170
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6;Cg-Pdx1<tm1(cre)Yasu>
Former Common name Pdx1 ires-Cre
H-2 Haplotype No Data
ES cell line V6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name Rabbit globin pA
Chromosome 5
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name Simian virus (SV40) pA
Chromosome 5
Common name No Data
Symbol description No Data
3 Symbol Ires
Symbol name internal ribosomal entry site (EMCV)
Chromosome 5
Common name No Data
Symbol description No Data
4 Symbol Pdx1
Symbol name pancreatic and duodenal homeobox 1
Chromosome 5
Common name No Data
Symbol description No Data
5 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome 5
Common name No Data
Symbol description No Data
6 Symbol rox
Symbol name D6 Bacteriophage rox site
Chromosome 5
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
7 Symbol puro
Symbol name puromycin resistance gene (Streptomyces alboniger)
Chromosome 5
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature Commun., 9, 2081 (2018).
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Yamada, Yasuhiro (Center for iPS Cell Research and Application, Kyoto University) Yamada, Yasuhiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC10171, B6;Cg-Gt(ROSA)26Sor<tm3(rtTA3)Yasu> Col1a1<tm4(tetO-OSKM,-mCherry)Yasu>

RBRC No. RBRC10171
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6;Cg-Gt(ROSA)26Sor<tm3(rtTA3)Yasu> Col1a1<tm4(tetO-OSKM,-mCherry)Yasu>
Former Common name Rosa26-LSL-rtTA3/ Col-tetO-OSKM
H-2 Haplotype No Data
ES cell line V6.5 [(C57BL/6J x 129S4/SvJae)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Col1a1
Symbol name collagen, type I, alpha 1
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System,
Tet system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature Commun., 9, 2081 (2018).
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Yamada, Yasuhiro (Center for iPS Cell Research and Application, Kyoto University) Yamada, Yasuhiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :