RBRC02704, B6.Cg-Tg(FucciS/G2/M)#474Bsi

RBRC No. RBRC02704
Type Transgene CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Tg(FucciS/G2/M)#474Bsi
Former Common name B6.Cg-Tg(Fucci)474Bsi, B6;B6D2-Tg(Fucci)474Bsi, B6;B6D2-Tg(Fucci)474Bsi
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Asako Sakaue-Sawano and Atsushi Miyawaki, RIKEN Brain Science Institute. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6N.
Strain description Transgenic mouse strains express fucci (fluorescent, ubiquitination-based cell cycle indicator) under the control of CAG promoter. These mice can be used to clearly visualize the cell cycle with fluorescence, i.e. S/G2/M phase nuclei green and those in G1 phase red in vivo. #474 (RBRC02704), #492 (RBRC02705), #504 (RBRC02706): Fucci-S/G2/M-Green (mAG-hGeminin(1/110)). #596 (RBRC02707), #610 (RBRC02708), #639 (RBRC02709): Fucci-G1-Red (mKO2-hCdt1(30/120)). For whole body (various tissues): #504 and #596 (Cell 2008). For hematopoietic system (monocyte and macrophage): #474 and #610 (Development 2013). For lymphocytic system: #492 and 639 (PNAS 2010). #474 and #610 double transgenic mice (RBRC02817). #504 and #596 double transgenic mice (RBRC02892). Whole-body visualization of Fucci mice with a flashlight (PCR genotyping is also available.): Check the Fucci S/2G/M-Green expressing mice at 1-4 days after birth by using blue LED flashlight through a sharp-cut filter in the dark box. Check the Fucci G1-Red expressing mice within 1-4 days after birth by using green LED flashlight through a green band-cut filter in the dark box. The part with few hairs such as in the mouth and auricles is suitable for the observation of fluorescence. Visualizing device: blue LED flashlight (470 nm), S470/30 filter, 535AF26 filter, sharp-cut filter (500 or 520 nm), green band-cut filter (red filter). #474 and #504: Tg homozygous mice are viable and fertile. #596: Tg homozygous mice are probably lethal (details unknown). #492, #610 and #639: Viability and fertility of homozygous mice are unknown (probably decreased fertility, details unknown).
Colony maintenance Carrier x Carrier [or Crossing to C57BL/6NJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol GMNN
Symbol name geminin (human)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
2 Symbol mAG
Symbol name monomeric Azami Green (Coral)
Chromosome UN
Common name No Data
Symbol description No Data
References Cell. 2008 Feb 8;132(3):487-98.

Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
PLOS ONE (2013) doi: 10.1371/journal.pone.0073801. Development (2013) doi:10.1242/dev.099226.
The RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT agrees to acknowledge PROVIDER in all oral presentations and written publications as related to the MATERIAL and provide a copy of the publication to the DEPOSITOR. The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement regarding the usage of the CAG promoter specified by the DEVELOPER, Dr. Junichi Miyazaki of Osaka University.
Additional information
1 CAGGS MATERIAL TRANSFER AGREEMENT (Pdf)/ (Word)
Miyawaki Lab HP
2 Genetic Background
3 Genotyping protocol <PCR>
Depositor Miyawaki, Atsushi (RIKEN Center for Brain Science) Miyawaki, Atsushi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
Tracking of intertissue migration reveals the origins of tumor-infiltrating monocytes. Proc Natl Acad Sci U S A. (2014).(24825888)
Shand FH, Ueha S, Otsuji M, Koid SS, Shichino S, Tsukui T, Kosugi-Kanaya M, Abe J, Tomura M, Ziogas J, Matsushima K.
Fucci-guided purification of hematopoietic stem cells with high repopulating activity. Biochem Biophys Res Commun. (2014).(25534850)
Yo M, Sakaue-Sawano A, Noda S, Miyawaki A, Miyoshi H.
Involvement of receptor activator of nuclear factor kappa-B ligand-induced incomplete cytokinesis in polyploidization of osteoclasts. J. Biol. Chem. (2015).(26670608)
Takegahara N, Kim H, Mizuno H, Sakaue-Sawano A, Miyawaki A, Tomura M, Kanagawa O, Ishii M, Choi Y.
Microbiome Influences Prenatal and Adult Microglia in a Sex-Specific Manner. Cell172(3): 500-516 (2018).(29275859)
Morgane Sonia Thion, Donovan Low, Aymeric Silvin, Jinmiao Chen, Pauline Grisel, Jonas Schulte-Schrepping, Ronnie Blecher, Thomas Ulas, Paola Squarzoni, Guillaume Hoeffel, Fanny Coulpier, Eleni Siopi, Friederike Sophie David, Claus Scholz, Foo Shihui, Josephine Lum, Arlaine Anne Amoyo, Anis Larbi, Michael Poidinger, Anne Buttgereit, Pierre-Marie Lledo, Melanie Greter, Jerry Kok Yen Chan, Ido Amit, Marc Beyer, Joachim Ludwig Schultze, Andreas Schlitzer, Sven Pettersson, Florent Ginhoux, Sonia Garel.
Developmental Analysis of Bone Marrow Neutrophils Reveals Populations Specialized in Expansion, Trafficking, and Effector Functions. Immunity (2018).(29466759)
Evrard M, Kwok IWH, Chong SZ, Teng KWW, Becht E, Chen J, Sieow JL, Penny HL, Ching GC, Devi S, Adrover JM, Li JLY, Liong KH, Tan L, Poon Z, Foo S, Chua JW, Su IH, Balabanian K, Bachelerie F, Biswas SK, Larbi A, Hwang WYK, Madan V, Koeffler HP, Wong SC, Newell EW, Hidalgo A, Ginhoux F, Ng LG.

RBRC02705, B6.Cg-Tg(FucciS/G2/M)#492Bsi

RBRC No. RBRC02705
Type Transgene CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Tg(FucciS/G2/M)#492Bsi
Former Common name B6.Cg-Tg(Fucci)492Bsi, B6;B6D2-Tg(Fucci)492Bsi
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Asako Sakaue-Sawano and Atsushi Miyawaki, RIKEN Brain Science Institute. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6N.
Strain description Transgenic mouse strains express fucci (fluorescent, ubiquitination-based cell cycle indicator) under the control of CAG promoter. These mice can be used to clearly visualize the cell cycle with fluorescence, i.e. S/G2/M phase nuclei green and those in G1 phase red in vivo. #474 (RBRC02704), #492 (RBRC02705), #504 (RBRC02706): Fucci-S/G2/M-Green (mAG-hGeminin(1/110)). #596 (RBRC02707), #610 (RBRC02708), #639 (RBRC02709): Fucci-G1-Red (mKO2-hCdt1(30/120)). For whole body (various tissues): #504 and #596 (Cell 2008). For hematopoietic system (monocyte and macrophage): #474 and #610 (Development 2013). For lymphocytic system: #492 and 639 (PNAS 2010). #474 and #610 double transgenic mice (RBRC02817). #504 and #596 double transgenic mice (RBRC02892). Whole-body visualization of Fucci mice with a flashlight (PCR genotyping is also available.): Check the Fucci S/2G/M-Green expressing mice at 1-4 days after birth by using blue LED flashlight through a sharp-cut filter in the dark box. Check the Fucci G1-Red expressing mice within 1-4 days after birth by using green LED flashlight through a green band-cut filter in the dark box. The part with few hairs such as in the mouth and auricles is suitable for the observation of fluorescence. Visualizing device: blue LED flashlight (470 nm), S470/30 filter, 535AF26 filter, sharp-cut filter (500 or 520 nm), green band-cut filter (red filter). #474 and #504: Tg homozygous mice are viable and fertile. #596: Tg homozygous mice are probably lethal (details unknown). #492, #610 and #639: Viability and fertility of homozygous mice are unknown (probably decreased fertility, details unknown).
Colony maintenance Carrier x Noncarrier [C57BL/6NJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol GMNN
Symbol name geminin (human)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
2 Symbol mAG
Symbol name monomeric Azami Green (Coral)
Chromosome UN
Common name No Data
Symbol description No Data
References Cell. 2008 Feb 8;132(3):487-98.

Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement regarding the usage of the CAG promoter specified by the DEVELOPER, Dr. Junichi Miyazaki of Osaka University. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a non-for profit institution for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Proc. Natl. Acad. Sci. U S A., 107 (27): 12192-12197 (2010) designated by the DEPOSITOR is requested. The RECIPIENT agrees to acknowledge PROVIDER in all oral presentations and written publications as related to the MATERIAL and provide a copy of the publication to the DEPOSITOR.
Additional information
1 CAGGS MATERIAL TRANSFER AGREEMENT (Pdf)/ (Word)
Miyawaki Lab HP
2 Genetic Background
3 Genotyping protocol <PCR>
Depositor Miyawaki, Atsushi (RIKEN Center for Brain Science) Miyawaki, Atsushi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
T cell signaling. Antigen affinity, costimulation, and cytokine inputs sum linearly to amplify T cell expansion. Science.346(6213): 1123-7 (2014).(25430770)
Marchingo JM, Kan A, Sutherland RM, Duffy KR, Wellard CJ, Belz GT, Lew AM, Dowling MR, Heinzel S, Hodgkin PD.
T cell signaling. Antigen affinity, costimulation, and cytokine inputs sum linearly to amplify T cell expansion. Science.346(6213): 1123-7 (2014).(25430770)
Marchingo JM, Kan A, Sutherland RM, Duffy KR, Wellard CJ, Belz GT, Lew AM, Dowling MR, Heinzel S, Hodgkin PD.

RBRC02708, B6.Cg-Tg(FucciG1)#610Bsi

RBRC No. RBRC02708
Type Transgene CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Tg(FucciG1)#610Bsi
Former Common name B6.Cg-Tg(Fucci)610Bsi, B6;B6D2-Tg(Fucci)610Bsi
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Asako Sakaue-Sawano and Atsushi Miyawaki, RIKEN Brain Science Institute. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6N.
Strain description Transgenic mouse strains express fucci (fluorescent, ubiquitination-based cell cycle indicator) under the control of CAG promoter. These mice can be used to clearly visualize the cell cycle with fluorescence, i.e. S/G2/M phase nuclei green and those in G1 phase red in vivo. #474 (RBRC02704), #492 (RBRC02705), #504 (RBRC02706): Fucci-S/G2/M-Green (mAG-hGeminin(1/110)). #596 (RBRC02707), #610 (RBRC02708), #639 (RBRC02709): Fucci-G1-Red (mKO2-hCdt1(30/120)). For whole body (various tissues): #504 and #596 (Cell 2008). For hematopoietic system (monocyte and macrophage): #474 and #610 (Development 2013). For lymphocytic system: #492 and 639 (PNAS 2010). #474 and #610 double transgenic mice (RBRC02817). #504 and #596 double transgenic mice (RBRC02892). Whole-body visualization of Fucci mice with a flashlight (PCR genotyping is also available.): Check the Fucci S/2G/M-Green expressing mice at 1-4 days after birth by using blue LED flashlight through a sharp-cut filter in the dark box. Check the Fucci G1-Red expressing mice within 1-4 days after birth by using green LED flashlight through a green band-cut filter in the dark box. The part with few hairs such as in the mouth and auricles is suitable for the observation of fluorescence. Visualizing device: blue LED flashlight (470 nm), S470/30 filter, 535AF26 filter, sharp-cut filter (500 or 520 nm), green band-cut filter (red filter). #474 and #504: Tg homozygous mice are viable and fertile. #596: Tg homozygous mice are probably lethal (details unknown). #492, #610 and #639: Viability and fertility of homozygous mice are unknown (probably decreased fertility, details unknown).
Colony maintenance Carrier x Noncarrier [C57BL/6NJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol CDT1
Symbol name chromatin licensing and DNA replication factor (human)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
2 Symbol mKO2
Symbol name monomeric Kusabira Orange (Coral)
Chromosome UN
Common name No Data
Symbol description No Data
References Cell. 2008 Feb 8;132(3):487-98.

Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Development (2013) doi:10.1242/dev.099226.
The RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT agrees to acknowledge PROVIDER in all oral presentations and written publications as related to the MATERIAL and provide a copy of the publication to the DEPOSITOR.
Additional information
1 CAGGS MATERIAL TRANSFER AGREEMENT (Pdf)/ (Word)
Miyawaki Lab HP
2 Genetic Background
3 Genotyping protocol <PCR>
Depositor Miyawaki, Atsushi (RIKEN Center for Brain Science) Miyawaki, Atsushi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
Fucci-guided purification of hematopoietic stem cells with high repopulating activity. Biochem Biophys Res Commun. (2014).(25534850)
Yo M, Sakaue-Sawano A, Noda S, Miyawaki A, Miyoshi H.

RBRC02709, B6.Cg-Tg(FucciG1)#639Bsi

RBRC No. RBRC02709
Type Transgene CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Tg(FucciG1)#639Bsi
Former Common name B6.Cg-Tg(Fucci)639Bsi, B6;B6D2-Tg(Fucci)639Bsi
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Asako Sakaue-Sawano and Atsushi Miyawaki, RIKEN Brain Science Institute. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6N.
Strain description Transgenic mouse strains express fucci (fluorescent, ubiquitination-based cell cycle indicator) under the control of CAG promoter. These mice can be used to clearly visualize the cell cycle with fluorescence, i.e. S/G2/M phase nuclei green and those in G1 phase red in vivo. #474 (RBRC02704), #492 (RBRC02705), #504 (RBRC02706): Fucci-S/G2/M-Green (mAG-hGeminin(1/110)). #596 (RBRC02707), #610 (RBRC02708), #639 (RBRC02709): Fucci-G1-Red (mKO2-hCdt1(30/120)). For whole body (various tissues): #504 and #596 (Cell 2008). For hematopoietic system (monocyte and macrophage): #474 and #610 (Development 2013). For lymphocytic system: #492 and 639 (PNAS 2010). #474 and #610 double transgenic mice (RBRC02817). #504 and #596 double transgenic mice (RBRC02892). Whole-body visualization of Fucci mice with a flashlight (PCR genotyping is also available.): Check the Fucci S/2G/M-Green expressing mice at 1-4 days after birth by using blue LED flashlight through a sharp-cut filter in the dark box. Check the Fucci G1-Red expressing mice within 1-4 days after birth by using green LED flashlight through a green band-cut filter in the dark box. The part with few hairs such as in the mouth and auricles is suitable for the observation of fluorescence. Visualizing device: blue LED flashlight (470 nm), S470/30 filter, 535AF26 filter, sharp-cut filter (500 or 520 nm), green band-cut filter (red filter). #474 and #504: Tg homozygous mice are viable and fertile. #596: Tg homozygous mice are probably lethal (details unknown). #492, #610 and #639: Viability and fertility of homozygous mice are unknown (probably decreased fertility, details unknown).
Colony maintenance Carrier x Noncarrier [C57BL/6NJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol CDT1
Symbol name chromatin licensing and DNA replication factor (human)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
2 Symbol mKO2
Symbol name monomeric Kusabira Orange (Coral)
Chromosome UN
Common name No Data
Symbol description No Data
References Cell. 2008 Feb 8;132(3):487-98.

Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
PLOS ONE, Published: September 16, (2013). Contrasting Quiescent G0 Phase with Mitotic Cell Cycling in the Mouse Immune System
Michio Tomura equal contributor, Asako Sakaue-Sawano equal contributor, Yoshiko Mori equal contributor, Mitsuyo Takase-Utsugi, Akihiro Hata, Kenji Ohtawa, Osami Kanagawa, Atsushi Miyawaki.
The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement regarding the usage of the CAG promoter specified by the DEVELOPER, Dr. Junichi Miyazaki of Osaka University. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a non-for profit institution for a not-for-profit research. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature (Proc. Natl. Acad. Sci. U S A., 107 (27): 12192-12197 (2010) designated by the DEPOSITOR is requested. The RECIPIENT agrees to acknowledge PROVIDER in all oral presentations and written publications as related to the MATERIAL and provide a copy of the publication to the DEPOSITOR.
Additional information
1 CAGGS MATERIAL TRANSFER AGREEMENT (Pdf)/ (Word)
Miyawaki Lab HP
2 Genetic Background
3 Genotyping protocol <PCR>
Depositor Miyawaki, Atsushi (RIKEN Center for Brain Science) Miyawaki, Atsushi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Stretched cell cycle model for proliferating lymphocytes. Proc Natl Acad Sci U S A. (2014).(24733943)
Dowling MR, Kan A, Heinzel S, Zhou JH, Marchingo JM, Wellard CJ, Markham JF, Hodgkin PD.
Tracking of intertissue migration reveals the origins of tumor-infiltrating monocytes. Proc Natl Acad Sci U S A. (2014).(24825888)
Shand FH, Ueha S, Otsuji M, Koid SS, Shichino S, Tsukui T, Kosugi-Kanaya M, Abe J, Tomura M, Ziogas J, Matsushima K.
T cell signaling. Antigen affinity, costimulation, and cytokine inputs sum linearly to amplify T cell expansion. Science.346(6213): 1123-7 (2014).(25430770)
Marchingo JM, Kan A, Sutherland RM, Duffy KR, Wellard CJ, Belz GT, Lew AM, Dowling MR, Heinzel S, Hodgkin PD.
Involvement of receptor activator of nuclear factor kappa-B ligand-induced incomplete cytokinesis in polyploidization of osteoclasts. J. Biol. Chem. (2015).(26670608)
Takegahara N, Kim H, Mizuno H, Sakaue-Sawano A, Miyawaki A, Tomura M, Kanagawa O, Ishii M, Choi Y.

RBRC02817, B6.Cg-Tg(FucciS/G2/M)#474Bsi Tg(FucciG1)#610Bsi

RBRC No. RBRC02817
Type Transgene CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Tg(FucciS/G2/M)#474Bsi Tg(FucciG1)#610Bsi
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Asako Sakaue-Sawano and Atsushi Miyawaki, RIKEN Brain Science Institute. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6N.
Strain description Transgenic mouse strains express fucci (fluorescent, ubiquitination-based cell cycle indicator) under the control of CAG promoter. These mice can be used to clearly visualize the cell cycle with fluorescence, i.e. S/G2/M phase nuclei green and those in G1 phase red in vivo. #474 (RBRC02704), #492 (RBRC02705), #504 (RBRC02706): Fucci-S/G2/M-Green (mAG-hGeminin(1/110)). #596 (RBRC02707), #610 (RBRC02708), #639 (RBRC02709): Fucci-G1-Red (mKO2-hCdt1(30/120)). For whole body (various tissues): #504 and #596 (Cell 2008). For hematopoietic system (monocyte and macrophage): #474 and #610 (Development 2013). For lymphocytic system: #492 and 639 (PNAS 2010). #474 and #610 double transgenic mice (RBRC02817). #504 and #596 double transgenic mice (RBRC02892). Whole-body visualization of Fucci mice with a flashlight (PCR genotyping is also available.): Check the Fucci S/2G/M-Green expressing mice at 1-4 days after birth by using blue LED flashlight through a sharp-cut filter in the dark box. Check the Fucci G1-Red expressing mice within 1-4 days after birth by using green LED flashlight through a green band-cut filter in the dark box. The part with few hairs such as in the mouth and auricles is suitable for the observation of fluorescence. Visualizing device: blue LED flashlight (470 nm), S470/30 filter, 535AF26 filter, sharp-cut filter (500 or 520 nm), green band-cut filter (red filter). #474 and #504: Tg homozygous mice are viable and fertile. #596: Tg homozygous mice are probably lethal (details unknown). #492, #610 and #639: Viability and fertility of homozygous mice are unknown (probably decreased fertility, details unknown).
Colony maintenance #474: Carrier x Noncarrier; #610: Carrier x Nocarrier [Crossing with #474 (RBRC02704) and #610 (RBRC02708)]
Health Report
Gene Details
Promoter No Data
1 Symbol CDT1
Symbol name chromatin licensing and DNA replication factor (human)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol GMNN
Symbol name geminin (human)
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol mAG
Symbol name monomeric Azami Green (Coral)
Chromosome UN
Common name No Data
Symbol description No Data
4 Symbol mKO2
Symbol name monomeric Kusabira Orange (Coral)
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
PLOS ONE (2013) doi: 10.1371/journal.pone.0073801. Development (2013) doi:10.1242/dev.099226.
The RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT agrees to acknowledge PROVIDER in all oral presentations and written publications as related to the MATERIAL and provide a copy of the publication to the DEPOSITOR. The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement regarding the usage of the CAG promoter specified by the DEVELOPER, Dr. Junichi Miyazaki of Osaka University.
Additional information
1 CAGGS MATERIAL TRANSFER AGREEMENT (Pdf)/ (Word)
Miyawaki Lab HP
2 Genotyping protocol <PCR>
Depositor Miyawaki, Atsushi (RIKEN Center for Brain Science) Miyawaki, Atsushi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
Fucci-guided purification of hematopoietic stem cells with high repopulating activity. Biochem Biophys Res Commun. (2014).(25534850)
Yo M, Sakaue-Sawano A, Noda S, Miyawaki A, Miyoshi H.

RBRC09364, B6;129S-Igs7<tm92.1(tetO-YCX2.60)Hze>/J

RBRC No. RBRC09364
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129S-Igs7<tm92.1(tetO-YCX2.60)Hze>/J
Former Common name Ai92(TITL-YCX2.60)
H-2 Haplotype No Data
ES cell line G4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 polyA
Chromosome 9
Common name No Data
Symbol description No Data
2 Symbol F3
Symbol name yeast FRT (flipase recombination target) site
Chromosome 9
Common name No Data
Symbol description No Data
3 Symbol F5
Symbol name yeast FRT (flipase recombination target) site
Chromosome 9
Common name No Data
Symbol description No Data
4 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 9
Common name No Data
Symbol description No Data
5 Symbol Igs7
Symbol name intergenic site 7
Chromosome 9
Common name No Data
Symbol description No Data
6 Symbol WPRE
Symbol name Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)
Chromosome 9
Common name No Data
Symbol description No Data
7 Symbol loxP
Symbol name phage P1 loxP
Chromosome 9
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
8 Symbol Hyg
Symbol name hygromycin phasphotransferase (E. coli)
Chromosome 9
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System,
Tet system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neuron., 85(5): 942-958 (2015).
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT agrees to acknowledge PROVIDER in all oral presentations and written publications as related to the MATERIAL and provide a copy of the publication to the DEPOSITOR.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Miyawaki Lab HP
Depositor Miyawaki, Atsushi (RIKEN Center for Brain Science) Miyawaki, Atsushi
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09365, STOCK Gt(ROSA)26Sor<tm5(ACTB-tTA)Luo>Igs7<tm92.1(tetO-YCX2.60)Hze>/J

RBRC No. RBRC09365
Type Targeted MutationCartagena
Species Mus musculus
Strain name STOCK Gt(ROSA)26Sor<tm5(ACTB-tTA)Luo>Igs7<tm92.1(tetO-YCX2.60)Hze>/J
Former Common name ROSA26-ZtTA;Ai92(TITL-YCX2.60)
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name SV40 polyA
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol b-geo
Symbol name No Data
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol tTA
Symbol name tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol No Data
Symbol name SV40 polyA
Chromosome 9
Common name No Data
Symbol description No Data
7 Symbol F3
Symbol name yeast FRT (flipase recombination target) site
Chromosome 9
Common name No Data
Symbol description No Data
8 Symbol F5
Symbol name yeast FRT (flipase recombination target) site
Chromosome 9
Common name No Data
Symbol description No Data
9 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 9
Common name No Data
Symbol description No Data
10 Symbol Igs7
Symbol name intergenic site 7
Chromosome 9
Common name No Data
Symbol description No Data
11 Symbol WPRE
Symbol name Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)
Chromosome 9
Common name No Data
Symbol description No Data
12 Symbol loxP
Symbol name phage P1 loxP
Chromosome 9
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
13 Symbol Hyg
Symbol name hygromycin phasphotransferase (E. coli)
Chromosome 9
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System,
Tet system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neuron., 85(5): 942-958 (2015).
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT agrees to acknowledge PROVIDER in all oral presentations and written publications as related to the MATERIAL and provide a copy of the publication to the DEPOSITOR.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Miyawaki Lab HP
2 Genotyping protocol <PCR>
Depositor Miyawaki, Atsushi (RIKEN Center for Brain Science) Miyawaki, Atsushi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :