RBRC03798, B6.129X1-Sept4<tm1Ksh>

RBRC No. RBRC03798
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129X1-Sept4<tm1Ksh>
Former Common name B6J-Sept4 KO
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Sept4
Symbol name septin 4
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name No Data
Chromosome 11
Common name No Data
Symbol description No Data
References Dev Cell. 2005 Mar;8(3):343-52.
J Hepatol. 2008 Nov;49(5):768-78. Epub 2008 Jul 15.
Mol Cell Biol 2005 Dec;25(24):10965-78.
Neuron. 2007 Feb 15;53(4):519-33.

Cortical organization by the septin cytoskeleton is essential for structural and mechanical integrity of mammalian spermatozoa.
Cortical organization by the septin cytoskeleton is essential for structural and mechanical integrity of mammalian spermatozoa.
Cortical organization by the septin cytoskeleton is essential for structural and mechanical integrity of mammalian spermatozoa.
Cortical organization by the septin cytoskeleton is essential for structural and mechanical integrity of mammalian spermatozoa.
Cortical organization by the septin cytoskeleton is essential for structural and mechanical integrity of mammalian spermatozoa.
Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Dev. Cell., 8, 343-352 (2005).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kinoshita, Makoto (Nagoya University) Kinoshita, Makoto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC03799, C.129X1(B6)-Sept4<tm1Ksh>

RBRC No. RBRC03799
Type Targeted MutationCartagena
Species Mus musculus
Strain name C.129X1(B6)-Sept4<tm1Ksh>
Former Common name BALB/c-Sept4 KO
H-2 Haplotype No Data
Background strain BALB/cCrSlc
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Sept4
Symbol name septin 4
Chromosome 11
Common name No Data
Symbol description No Data
References Dev Cell. 2005 Mar;8(3):343-52.
J Hepatol. 2008 Nov;49(5):768-78. Epub 2008 Jul 15.
Mol Cell Biol 2005 Dec;25(24):10965-78.
Neuron. 2007 Feb 15;53(4):519-33.

Cortical organization by the septin cytoskeleton is essential for structural and mechanical integrity of mammalian spermatozoa.
Cortical organization by the septin cytoskeleton is essential for structural and mechanical integrity of mammalian spermatozoa.
Cortical organization by the septin cytoskeleton is essential for structural and mechanical integrity of mammalian spermatozoa.
Cortical organization by the septin cytoskeleton is essential for structural and mechanical integrity of mammalian spermatozoa.
Cortical organization by the septin cytoskeleton is essential for structural and mechanical integrity of mammalian spermatozoa.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Dev. Cell., 8, 343-352 (2005).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kinoshita, Makoto (Nagoya University) Kinoshita, Makoto
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC03800, C57BL/6J-Tg(Prnp-Sept4)1Ksh

RBRC No. RBRC03800
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6J-Tg(Prnp-Sept4)1Ksh
Former Common name C57BL/6J-Sept4Tg
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Sept4
Symbol name septin 4
Chromosome UN
Common name No Data
Symbol description No Data
References Molecular Brain, (2013).
Dev Cell. 2005 Mar;8(3):343-52.
J Hepatol. 2008 Nov;49(5):768-78. Epub 2008 Jul 15.
Mol Cell Biol 2005 Dec;25(24):10965-78.
Neuron. 2007 Feb 15;53(4):519-33.

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neuron, 53, 519-533 (2007).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kinoshita, Makoto (Nagoya University) Kinoshita, Makoto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04894, C57BL/6-Cdc42ep4<tm1Ksh>

RBRC No. RBRC04894
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6-Cdc42ep4<tm1Ksh>
Former Common name B6-Cdc42ep4 floxed
H-2 Haplotype No Data
ES cell line RENKA [C57BL/6NCrlCrlj]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Makoto Kinoshita, Nagoya University Graduate School of Science in 2009.
Strain description No Data
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj] Homozygous mutant mice are viable and fertile.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Cdc42ep4
Symbol name CDC42 effector protein (Rho GTPase binding) 4
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 11
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 11
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
6 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References Unpublished

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Commun. 6:10090 (2015).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kinoshita, Makoto (Nagoya University) Kinoshita, Makoto
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05390, C57BL/6-Sept7<tm1Ksh>

RBRC No. RBRC05390
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6-Sept7<tm1Ksh>
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Makoto Kinoshita, Nagoya University Graduate School of Science in 2007-8. C57BL/6 background. A frt flanked neo cassette was removed by FRP meditated recombination.
Strain description Sept7 floxed mice. Exon 3 of Sept7 gene was flanked by loxP sites.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Sept7
Symbol name septin 7
Chromosome 9
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 9
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 9
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat Commun. 2013;4:2532. doi: 10.1038/ncomms3532
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kinoshita, Makoto (Nagoya University) Kinoshita, Makoto
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06467, C57BL/6-Tg(Prnp-Sirt1,Cryaa-EGFP)1Ksh

RBRC No. RBRC06467
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(Prnp-Sirt1,Cryaa-EGFP)1Ksh
Former Common name PrP-Sirt1 Tg, B6-Prnp-Sirt1/Cryaa-GFP Tg
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Makoto Kinoshita, Nagoya University Graduate School of Science (2008-9). Prnp-Sirt1 and alpha A-crystallin EGFP transcription units were co-injected into the C57BL/6J mouse oocytes.
Strain description PrP-Sirt1 transgenic mice having a mouse Sirt1 gene under the control of mouse prion gene promoter (MoPrP). The MoPrP-Sirt1 transcription unit was co-injected with a mouse alpha A-crystallin promoter-driven EGFP cDNA.
Colony maintenance Carrier x Nocarrier [C57BL/6JJmsSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Cryaa promoter
2 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Prnp promoter
3 Symbol Sirt1
Symbol name sirtuin 1
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Fluorescent Proteins/lacZ System,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol Brain. 2014 Aug 29;7(1):62. doi: 10.1186/s13041-014-0062-1.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
For profit use of the BIOLOGICAL RESSOURCE, the RECIPIENT must contact with the Intellectual Property Office, Nagoya University to obtain a prior written consent.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Mouse of the Month Feb 2015
Depositor Kinoshita, Makoto (Nagoya University) Kinoshita, Makoto
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09539, C57BL/6-Cdc42ep4<tm1.1Ksh>

RBRC No. RBRC09539
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6-Cdc42ep4<tm1.1Ksh>
Former Common name B6-Cdc42ep4KO
H-2 Haplotype No Data
ES cell line RENKA [C57BL/6NCrlCrlj]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Dr. Makoto Kinoshita/ Nagoya University Graduate School of Science
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Cdc42ep4
Symbol name CDC42 effector protein (Rho GTPase binding) 4
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 11
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Commun. 6:10090 (2015).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Kinoshita, Makoto (Nagoya University) Kinoshita, Makoto
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months