RBRC09730, B6.Cg-Braf<tm1Tumg>

RBRC No. RBRC09730
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Braf<tm1Tumg>
Former Common name Braf Q241R Neo/+ (C57BL/6 background)
H-2 Haplotype No Data
ES cell line Bruce 4 [B6.Cg-Thy1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Yoko Aoki and Shin-ichi Inoue, Tohoku University School of Medicine in 2014. BRUCE-4 ES cells were used. Mice were maintained by sister-brother mating.
Strain description Knock-in mutants in which Braf-mutated protein (BrafQ241R) is expressed in a Cre-dependent manner. Q241R mutation in mouse Braf corresponds to Q257R in human BRAF, the most frequent mutation in the cardio-facio-cutaneous (CFC) syndrome. BrafQ241R heterozygous mice in the ICR genetic background are available as RBRC09731.
Health Report
Gene Details
Promoter No Data
1 Symbol Braf
Symbol name Braf transforming gene
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name No Data
Symbol description No Data
References No Data

Research applications Cancer Research,
Cell Biology Research,
Cre/loxP system,
FLP/frt system,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Human Molecular Genetics, 23, 6553-6566 (2014).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Aoki, Yoko (Tohoku University) Aoki, Yoko
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09731, ICR.Cg-Braf<tm1.1Tumg>

RBRC No. RBRC09731
Type Targeted MutationCartagena
Species Mus musculus
Strain name ICR.Cg-Braf<tm1.1Tumg>
Former Common name Braf Q241R/+ ICR (Braf Q241R knock-in mice; ICR background)
H-2 Haplotype No Data
ES cell line Bruce 4 [B6.Cg-Thy1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Yoko Aoki and Shin-ichi Inoue, Tohoku University School of Medicine in 2014. BRUCE-4 ES cells were used. RBRC09730 was crossed with CAG-Cre and then backcrossed to ICR for 11 generations.
Strain description Braf-mutated protein (BrafQ241R) knock-in mice in ICR genetic background. Q241R mutation in mouse Braf corresponds to Q257R in human BRAF, the most frequent mutation in the cardio-facio-cutaneous (CFC) syndrome. Conditional BrafQ241R knock-in mice are available as RBRC09730.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Braf
Symbol name Braf transforming gene
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
References No Data

Research applications Cancer Research,
Cell Biology Research,
Cre/loxP system,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Human Molecular Genetics, 23, 6553-6566 (2014). Human Molecular Genetics, 24, 7349-7360 (2015).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Aoki, Yoko (Tohoku University) Aoki, Yoko
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09907, 129.Cg-Braf<tm1Tumg>

RBRC No. RBRC09907
Type Targeted MutationCartagena
Species Mus musculus
Strain name 129.Cg-Braf<tm1Tumg>
Former Common name Braf Q241R Neo/+ (129 background)
H-2 Haplotype No Data
ES cell line Bruce 4 [B6.Cg-Thy1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description Knock-in mutants in which Braf-mutated protein (BrafQ241R) is expressed in a Cre-dependent manner. Q241R mutation in mouse Braf corresponds to Q257R in human BRAF, the most frequent mutation in the cardio-facio-cutaneous (CFC) syndrome. BrafQ241R heterozygous mice in the ICR genetic background are available as RBRC09731.
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 polyA signal
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol Braf
Symbol name Braf transforming gene
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol Tyr
Symbol name tyrosinase
Chromosome 7
Common name No Data
Symbol description No Data
7 Symbol Tyr
Symbol name tyrosinase
Chromosome 7
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
8 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name No Data
Symbol description No Data
References No Data

Research applications Cancer Research,
Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Hum. Mol. Genet., 23, 6553-6566 (2014).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Aoki, Yoko (Tohoku University) Aoki, Yoko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC09908, C.Cg-Braf<tm1Tumg>

RBRC No. RBRC09908
Type Targeted MutationCartagena
Species Mus musculus
Strain name C.Cg-Braf<tm1Tumg>
Former Common name Braf Q241R Neo/+ (BALB/c background)
H-2 Haplotype No Data
ES cell line Bruce 4 [B6.Cg-Thy1]
Background strain BALB/cAnNCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 polyA signal
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol Braf
Symbol name Braf transforming gene
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Hum. Mol. Genet., 23, 6553-6566 (2014).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Aoki, Yoko (Tohoku University) Aoki, Yoko
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC10843, B6.Cg-Rit1<tm1(A57G)Tumg>

RBRC No. RBRC10843
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Rit1<tm1(A57G)Tumg>
Former Common name Rit1A57G Neo/+ (C57BL/6J background)
H-2 Haplotype No Data
ES cell line Bruce 4 [B6.Cg-Thy1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name Adenovirus SA(splice acceptor site)-stop
Chromosome 3
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name Polyomavirus SV40 polyA signal
Chromosome 3
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 3
Common name No Data
Symbol description No Data
4 Symbol Rit1
Symbol name Ras-like without CAAX 1
Chromosome 3
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 3
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
6 Symbol neo
Symbol name No Data
Chromosome 3
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
EBioMedicine, 42:43-53 (2019).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Aoki, Yoko (Tohoku University) Aoki, Yoko
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC10844, B6.Cg-Rit1<tm1.1(A57G)Tumg>

RBRC No. RBRC10844
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Rit1<tm1.1(A57G)Tumg>
Former Common name Rit1A57G/+ (Rit1 A57G knock-in mice; C57BL/6J background)
H-2 Haplotype No Data
ES cell line Bruce 4 [B6.Cg-Thy1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Rit1
Symbol name Ras-like without CAAX 1
Chromosome 3
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 3
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
EBioMedicine, 42:43-53 (2019).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Aoki, Yoko (Tohoku University) Aoki, Yoko
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC10866, B6.Cg-Hras<tm1.1(G12S)Tumg>

RBRC No. RBRC10866
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Hras<tm1.1(G12S)Tumg>
Former Common name HrasG12S/+ (Hras G12S knock-in mice; C57BL/6J background)
H-2 Haplotype No Data
ES cell line Bruce 4 [B6.Cg-Thy1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Hras
Symbol name Harvey rat sarcoma virus oncogene
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
EBioMedicine. 2018 27:138-150
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICALRESOURCE.
Additional information
1 No Data
Depositor Aoki, Yoko (Tohoku University) Aoki, Yoko
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :