RBRC05522, B6.129S7-Sox9<tm1(cre/ERT2)Haak>

RBRC No. RBRC05522
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129S7-Sox9<tm1(cre/ERT2)Haak>
Former Common name Sox9-CreERT2 KI, Sox9-CreERT2 knock-in, B6-Sox9 KI/CreER
H-2 Haplotype No Data
ES cell line AB1 [129S7/SvEvBrd-Hprt<+>]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Haruhiko Akiyama, The University of Texas MD Anderson Cancer Center. The knock-in vector was electroporated into AB1 ES cells. The mutant mice were crossed to C57BL/6.
Strain description Sox9-CreER knock-in mice. These mice have a tamoxifen-inducible cre (CreERT2) at the Sox9 locus. IRES-CreERT2-pA and FRT-PGK-neo-bpA cassettes were inserted into the 3' UTR of exon 3 of the Sox9 locus.
Colony maintenance Heterozygote x Wild-type [C57BL/6]
Health Report
Gene Details
Promoter No Data
1 Symbol ERT2
Symbol name estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 11
Common name No Data
Symbol description No Data
3 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 11
Common name No Data
Symbol description No Data
4 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 11
Common name No Data
Symbol description No Data
5 Symbol Sox9
Symbol name SRY-box containing gene 9
Chromosome 11
Common name No Data
Symbol description No Data
6 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome 11
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
7 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Developmental Biology Research,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genesis., 48, 635-644 (2010).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Haruhiko Akiyama, Kyoto University, is requested. Depositor retains ownership of the Biological Resource, including any Biological Resource contained in any modification. Any Biological Resource delivered pursuant to the Agreement is understood to be experimental in nature and may have hazardous properties. The DEPOSITOR MAKES NO REPRESENTATIONS AND EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED. THERE ARE NO EXPRESS OR IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR THAT THE USE OF THE BIOLOGICAL RESOURCE WILL NOT INFRINGE ANY PATENT, COPYRIGHT, TRADEMARK, OR OTHER PROPRIETARY RIGHTS. In no event shall Depositor be liable for any use by recipient of the Biological Resource for any loss, claim, damage or liability, of whatsoever kind of nature, which may arise from or in connection with the use, handling or storage of the Biological Resource. Except where limited by Federal law, or by the constitution and laws of the state governing the recipient, the recipient agrees to hold harmless Depositor and The University of Texas System and their Regents, officers, agents and employees, from any liability, loss or damage they may suffer as a result of claims, demands, costs or judgments against them arising out of recipient's use of the Material and the use by recipient of the results obtained from the Research. The Biological Resource and modifications containing the Biological Resource may only be transferred to non-profit institutions for academic research purposes.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor de Crombrugghe, Benoit (The University of Texas MD Anderson Cancer Center) de Crombrugghe, Benoit
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC05523, B6.129S7-Sox9<tm1(cre)Crm>

RBRC No. RBRC05523
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129S7-Sox9<tm1(cre)Crm>
Former Common name Sox9-Cre KI, Sox9-Cre knock-in, B6-Sox9 KI/Cre
H-2 Haplotype No Data
ES cell line AB1 [129S7/SvEvBrd-Hprt<+>]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Haruhiko Akiyama, The University of Texas MD Anderson Cancer Center. The knock-in vector was electroporated into AB1 ES cells. The mutant mice were crossed to C57BL/6.
Strain description Sox9-Cre knock-in mice. These mice have a cre recombinase gene at the Sox9 locus. An IRES-Cre-pA/frt-flanked PGK-neo-bpA cassette was inserted into the 3' UTR of exon 3 of the Sox9 locus.
Colony maintenance Heterozygote x Wild-type [C57BL/6]
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 11
Common name No Data
Symbol description No Data
3 Symbol Sox9
Symbol name SRY-box containing gene 9
Chromosome 11
Common name No Data
Symbol description No Data
4 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome 11
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name No Data
Symbol description No Data
References Proc Natl Acad Sci U S A 2005 Oct 11;102(41):14665-70. Epub 2005 Oct 3.

Osteo-chondroprogenitor cells are derived from Sox9 expressing precursors.
Research applications Cre/loxP system,
Developmental Biology Research,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Proc. Natl. Acad. Sci., U S A,, 102, :14665-14670 (2005).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Haruhiko Akiyama, Kyoto University, is requested. Depositor retains ownership of the Biological Resource, including any Biological Resource contained in any modification. Any Biological Resource delivered pursuant to the Agreement is understood to be experimental in nature and may have hazardous properties. The DEPOSITOR MAKES NO REPRESENTATIONS AND EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED. THERE ARE NO EXPRESS OR IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR THAT THE USE OF THE BIOLOGICAL RESOURCE WILL NOT INFRINGE ANY PATENT, COPYRIGHT, TRADEMARK, OR OTHER PROPRIETARY RIGHTS. In no event shall Depositor be liable for any use by recipient of the Biological Resource for any loss, claim, damage or liability, of whatsoever kind of nature, which may arise from or in connection with the use, handling or storage of the Biological Resource. Except where limited by Federal law, or by the constitution and laws of the state governing the recipient, the recipient agrees to hold harmless Depositor and The University of Texas System and their Regents, officers, agents and employees, from any liability, loss or damage they may suffer as a result of claims, demands, costs or judgments against them arising out of recipient's use of the Material and the use by recipient of the results obtained from the Research. The Biological Resource and modifications containing the Biological Resource may only be transferred to non-profit institutions for academic research purposes.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor de Crombrugghe, Benoit (The University of Texas MD Anderson Cancer Center) de Crombrugghe, Benoit
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05524, B6.Cg-Tg(Col1a1-cre)1Haak

RBRC No. RBRC05524
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(Col1a1-cre)1Haak
Former Common name Col1a1-Cre Tg, Col1a1-Cre transgenic, B6-Col1a1Cre Tg
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Haruhiko Akiyama, The University of Texas MD Anderson Cancer Center. The mice were crossed to C57BL/6.
Strain description Col1a1-Cre transgenic mice in which Cre recombinase is specifically expressed in osteoblast.
Colony maintenance Carrier x Noncarrier [C57BL/6NCrSlc]
Health Report
Gene Details
Promoter mouse Col1a1 promoter
1 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References J Bone Miner Res. 2008 Feb;23(2):271-7.

Osteoblast-targeted expression of Sfrp4 in mice results in low bone mass.
Research applications Cre/loxP system,
Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Bone Miner Res., 23, 271-277 (2008).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Haruhiko Akiyama, Kyoto University, is requested. Depositor retains ownership of the Biological Resource, including any Biological Resource contained in any modification. Any Biological Resource delivered pursuant to the Agreement is understood to be experimental in nature and may have hazardous properties. The DEPOSITOR MAKES NO REPRESENTATIONS AND EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED. THERE ARE NO EXPRESS OR IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR THAT THE USE OF THE BIOLOGICAL RESOURCE WILL NOT INFRINGE ANY PATENT, COPYRIGHT, TRADEMARK, OR OTHER PROPRIETARY RIGHTS. In no event shall Depositor be liable for any use by recipient of the Biological Resource for any loss, claim, damage or liability, of whatsoever kind of nature, which may arise from or in connection with the use, handling or storage of the Biological Resource. Except where limited by Federal law, or by the constitution and laws of the state governing the recipient, the recipient agrees to hold harmless Depositor and The University of Texas System and their Regents, officers, agents and employees, from any liability, loss or damage they may suffer as a result of claims, demands, costs or judgments against them arising out of recipient's use of the Material and the use by recipient of the results obtained from the Research. The Biological Resource and modifications containing the Biological Resource may only be transferred to non-profit institutions for academic research purposes.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor de Crombrugghe, Benoit (The University of Texas MD Anderson Cancer Center) de Crombrugghe, Benoit
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05525, B6.B6D2-Tg(Prrx1-Sox9,-lacZ)1Haak

RBRC No. RBRC05525
Type TransgeneCartagena
Species Mus musculus
Strain name B6.B6D2-Tg(Prrx1-Sox9,-lacZ)1Haak
Former Common name Prx1-Sox9 Tg, Prx1-Sox9 transgenic, B6-Prx1-Sox9/lacZ Tg
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Haruhiko Akiyama, The University of Texas MD Anderson Cancer Center. The transgene was injected into the pronuclei of (C57BL/6 x DBA/2)F1 fertilized eggs. The mutant mice were crossed to C57BL/6.
Strain description Prx1-Sox9/lacZ transgenic mice in which Sox9 is specifically expressed in limb bud mesenchyme. Prx1-Sox9/lacZ transgene contains a 2.4 kb of the Prx1 limb specific promoter encoding the 3xHA-epitope tagged Sox9 cDNA followe by an IRES lacZ. Tg mice exhibit pre- and postaxial extra-digit formation. lacZ expression was detected in the limb buds from E9.5
Colony maintenance Carrier x Noncarrier [C57BL/6NCrSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol HA
Symbol name human influenza virus HA (hemagglutinin) tag sequence
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome UN
Common name No Data
Symbol description No Data
4 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Prx1 promoter
5 Symbol SOX9
Symbol name SRY-box containing gene 9 (human)
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Developmental Biology Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Matrix Biol., 26, 224-233 (2007).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Haruhiko Akiyama, Kyoto University, is requested. Depositor retains ownership of the Biological Resource, including any Biological Resource contained in any modification. Any Biological Resource delivered pursuant to the Agreement is understood to be experimental in nature and may have hazardous properties. The DEPOSITOR MAKES NO REPRESENTATIONS AND EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED. THERE ARE NO EXPRESS OR IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR THAT THE USE OF THE BIOLOGICAL RESOURCE WILL NOT INFRINGE ANY PATENT, COPYRIGHT, TRADEMARK, OR OTHER PROPRIETARY RIGHTS. In no event shall Depositor be liable for any use by recipient of the Biological Resource for any loss, claim, damage or liability, of whatsoever kind of nature, which may arise from or in connection with the use, handling or storage of the Biological Resource. Except where limited by Federal law, or by the constitution and laws of the state governing the recipient, the recipient agrees to hold harmless Depositor and The University of Texas System and their Regents, officers, agents and employees, from any liability, loss or damage they may suffer as a result of claims, demands, costs or judgments against them arising out of recipient's use of the Material and the use by recipient of the results obtained from the Research. The Biological Resource and modifications containing the Biological Resource may only be transferred to non-profit institutions for academic research purposes.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor de Crombrugghe, Benoit (The University of Texas MD Anderson Cancer Center) de Crombrugghe, Benoit
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05651, B6;129-Sox9<tm1Haak>

RBRC No. RBRC05651
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129-Sox9<tm1Haak>
Former Common name Sox9-EGFP KI, Sox9-EGFP knock-in, B6-Sox9/GFP KI
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Haruhiko Akiyama, The University of Texas MD Anderson Cancer Center. A loxP-flanked PGK-neo-bpA was removed by cre-mediated recombination. C57BL/6 and 129 mixed background.
Strain description Sox9-EGFP knock-in mice. An IRES-EGFP-pA cassette was inserted into the 3' untranslated region in exon 3 of Sox9 gene via homologous recombination.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 11
Common name No Data
Symbol description No Data
3 Symbol Sox9
Symbol name SRY-box containing gene 9
Chromosome 11
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Developmental Biology Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Dev. Dyn., 238, 1100-1110 (2009).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and Dr. Haruhiko Akiyama, Kyoto University, is requested. Depositor retains ownership of the Biological Resource, including any Biological Resource contained in any modification. Any Biological Resource delivered pursuant to the Agreement is understood to be experimental in nature and may have hazardous properties. The DEPOSITOR MAKES NO REPRESENTATIONS AND EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED. THERE ARE NO EXPRESS OR IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR THAT THE USE OF THE BIOLOGICAL RESOURCE WILL NOT INFRINGE ANY PATENT, COPYRIGHT, TRADEMARK, OR OTHER PROPRIETARY RIGHTS. In no event shall Depositor be liable for any use by recipient of the Biological Resource for any loss, claim, damage or liability, of whatsoever kind of nature, which may arise from or in connection with the use, handling or storage of the Biological Resource. Except where limited by Federal law, or by the constitution and laws of the state governing the recipient, the recipient agrees to hold harmless Depositor and The University of Texas System and their Regents, officers, agents and employees, from any liability, loss or damage they may suffer as a result of claims, demands, costs or judgments against them arising out of recipient's use of the Material and the use by recipient of the results obtained from the Research. The Biological Resource and modifications containing the Biological Resource may only be transferred to non-profit institutions for academic research purposes.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor de Crombrugghe, Benoit (The University of Texas MD Anderson Cancer Center) de Crombrugghe, Benoit
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months