RBRC00407, B6.129P2-Tcrd<tm1Mom>/ItoRbrc

RBRC No. RBRC00407
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Tcrd<tm1Mom>/ItoRbrc
Former Common name TCR delta-KO, TCRd KO
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Donor strain 129P2/OlaHsd via E14 ES cell line
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Drs. S. Itohara, P. Mombaerts and S Tonegawa, Howard Hughes Medical Institute, Massachusetts Institute of Technology in 1993.
Strain description Developed by Drs. S. Itohara, P. Mombaerts and S Tonegawa, Howard Hughes Medical Institute, Massachusetts Institute of Technology in 1993.
Colony maintenance Sibling Mating
Homozygous mutant mice are viable and fertile.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Tcrd
Symbol name T-cell receptor delta chain
Chromosome 14
Common name Tcr delta, Tcrdelta
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 14
Common name neo; neomycin;
Symbol description No Data
References Cell. 1993 Feb 12;72(3):337-48.

T cell receptor delta gene mutant mice: independent generation of alpha beta T cells and programmed rearrangements of gamma delta TCR genes.
Research applications Cell Biology Research,
Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Itohara et al., Cell, 72, 337-348 (1993).
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Itohara, ShigeYoshi (RIKEN Center for Brain Science) Itohara, ShigeYoshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Involvement of NK1.1+ {gamma}{delta}T Cells in the IL-18 Plus IL-2-Induced Interstitial Lung Disease. Am J Respir Cell Mol Biol. (2011).(21257923)
Segawa S, Goto D, Yoshiga Y, Horikoshi M, Sugihara M, Hayashi T, Chino Y, Matsumoto I, Ito S, Sumida T.
The regulatory role of IFN-γ producing gammadelta T cells via the suppression of Th17 cell activity in bleomycin-induced pulmonary fibrosis. Clin. Exp. Immunol. (2016).(27083148)
Segawa S, Goto D, Iizuka A, Kaneko S, Yokosawa M, Kondo Y, Matsumoto I, Sumida T.

RBRC10322, C57BL/6J-Tg(Trpc4-cre)G383Stl

RBRC No. RBRC10322
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6J-Tg(Trpc4-cre)G383Stl
Former Common name Trpc4-Cre
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Science 353, 1536-1541 (2016).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Itohara, ShigeYoshi (RIKEN Center for Brain Science) Itohara, ShigeYoshi
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC10700, C57BL/6-App<tm1(T668A)Tsuz>

RBRC No. RBRC10700
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6-App<tm1(T668A)Tsuz>
Former Common name T668A delta neo (A/A)
H-2 Haplotype No Data
ES cell line MS12 [C57BL/6]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol App
Symbol name amyloid beta (A4) precursor protein
Chromosome 16
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 16
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
PLoS One. 2006 Dec 20;1:e51.
Additional information
1 No Data
Depositor Itohara, ShigeYoshi (RIKEN Center for Brain Science) Itohara, ShigeYoshi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC10716, C57BL/6J-Tg(Aqp4-cre)4Ito

RBRC No. RBRC10716
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6J-Tg(Aqp4-cre)4Ito
Former Common name Aqp4-Cre#4v
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 polyA
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
Depositor Itohara, ShigeYoshi (RIKEN Center for Brain Science) Itohara, ShigeYoshi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC10732, C57BL/6-Ntng1<tm2.1Ito>

RBRC No. RBRC10732
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6-Ntng1<tm2.1Ito>
Former Common name Ntng1 flox
H-2 Haplotype No Data
ES cell line MS12 [C57BL/6]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 3
Common name No Data
Symbol description No Data
2 Symbol Ntng1
Symbol name netrin G1
Chromosome 3
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 3
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 3
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Sci Rep. 2016 Jun 27;6:28750. doi: 10.1038/srep28750
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Itohara, ShigeYoshi (RIKEN Center for Brain Science) Itohara, ShigeYoshi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC10748, C57BL/6-Rapgef4<tm1Ito>

RBRC No. RBRC10748
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6-Rapgef4<tm1Ito>
Former Common name CGII-flox
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 2
Common name No Data
Symbol description No Data
2 Symbol Rapgef4
Symbol name Rap guanine nucleotide exchange factor (GEF) 4
Chromosome 2
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 2
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 2
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol. Brain, 8:38 (2015).
Additional information
1 No Data
Depositor Itohara, ShigeYoshi (RIKEN Center for Brain Science) Itohara, ShigeYoshi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC00408, B6.129P2-Gfap<tm1Ito>/ItoRbrc

RBRC No. RBRC00408
Type Targeted Mutation CongenicCartagena wks
Species Mus musculus
Strain name B6.129P2-Gfap<tm1Ito>/ItoRbrc
Former Common name Gfap KO, GFAP-KO-clone 27
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Donor strain 129P2/OlaHsd via E14 ES cell line
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype No Data
Strain development Developed by Shigeyoshi Itohara, Institute for Virus Research, Kyoto University in 1995. The construct was electoroporated into E14 ES cells derived from 129P2/OlaHsd. The mutant mice were backcrossed to C57BL/6J.
Strain description Gfap knockout mice. Immediately downstream of the endogenous start codon of Gfap was replaced with a cassette, containing a promoterless lacZ and pgk-neo. Gfap, glial fibrillary acidic protein is an intermediate filament protein specifically expressed in astrocytes. Homozygous mutant mice develop normally and not show any detectable abnormalities in the anatomy of the brain. But, exhibit a impairment of long-term depression (LTD) and PF-Purkinje cell synapses and eyeblink, suggested that the Gfap is required for communications between Bergmann glia and Purkinje cells.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]. Homozygous mutant mice are viable and fertile.
Health Report
Gene Details
Promoter No Data
1 Symbol Gfap
Symbol name glial fibrillary acidic protein
Chromosome 11
Common name No Data
Symbol description No Data
Promoter endogenous start codon
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 11
Common name No Data
Symbol description E. coli beta galactosidase
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References Neuron. 1995 Jan;14(1):29-41.

Research applications Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Gomi et al., Neuron, 14, 29-41 (1995).
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Itohara, ShigeYoshi (RIKEN Center for Brain Science) Itohara, ShigeYoshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Allele-specific nuclear positioning of the monoallelically expressed astrocyte marker GFAP. Genes Dev.22(4): 489-98 (2008).(18281462)
Takizawa T, Gudla PR, Guo L, Lockett S, Misteli T.

RBRC10598, C57BL/6J-Tg(Slc6a4-cre)208Ito

RBRC No. RBRC10598
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6J-Tg(Slc6a4-cre)208Ito
Former Common name 5HTT-Cre, Sert-Cre, SLC6A4-Cre
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 polyA
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
4 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Arakawa et al. J Neurosci. 2014;34:12001-12014.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 No Data
Depositor Itohara, ShigeYoshi (RIKEN Center for Brain Science) Itohara, ShigeYoshi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC10701, C57BL/6-App<tm1.1(T668D)Ito>

RBRC No. RBRC10701
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6-App<tm1.1(T668D)Ito>
Former Common name T668D delta neo
H-2 Haplotype No Data
ES cell line MS12 [C57BL/6]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol App
Symbol name amyloid beta (A4) precursor protein
Chromosome 16
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 16
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
後日指定
Additional information
1 No Data
Depositor Itohara, ShigeYoshi (RIKEN Center for Brain Science) Itohara, ShigeYoshi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC10717, C57BL/6J-Tg(Aqp4-cre)5Ito

RBRC No. RBRC10717
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6J-Tg(Aqp4-cre)5Ito
Former Common name Aqp4-Cre#5A
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 polyA
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
Depositor Itohara, ShigeYoshi (RIKEN Center for Brain Science) Itohara, ShigeYoshi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months