RBRC00702, C57BL/6-TgH(Zic1)

RBRC No. RBRC00702
Type Targeted MutationCartagena 20wks,♀
Species Mus musculus
Strain name C57BL/6-TgH(Zic1)
Former Common name No Data
H-2 Haplotype No Data
ES cell line J1 [129S4/SvJae]
Donor strain 129S4/SvJae via J1 ES cell line
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype No Data
Strain development Developed by Aruga J (Aruga J et al. J Neurosci 18, 284-293, 1998).
The mice were frozen-stored at 2-cell stage after in vitro fertilization between a heterozygous male and C57BL/6J females. Live mice were recovered and born on April 28, 2003.
Deposited to RIKEN BioResource Center, RIKEN Tsukuba Institute in 2002.
Strain description Zic genes encode zinc finger proteins. These genes are homologs of the Drosophila pair-rule gene odd-paired. This strain was developed to clarify the function of Zic1 gene. Homozygous Zic1 deficient mice show remarkable ataxia during postnatal development, and die within 1 month. Their cerebella are hypoplastic and missing a lobule in the anterior lobe. A bromodeoxyuridine labeling study indicated a reduction both in the proliferating cell fraction in the external germinal layer (EGL), from embryonic day14, and in forward movement of the EGL (Aruga J et al. J Neurosci 18, 284-293, 1998).
Colony maintenance Mice were maintained by successive backcrossing heterozygotes to C57BL/6 mice.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Zic1
Symbol name zinc finger protein of the cerebellum 1
Chromosome 9
Common name zinc finger protein of the cerebellum 1
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 9
Common name neo; neomycin;
Symbol description No Data
References J Neurosci. 1998 Jan 1;18(1):284-93.

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neurosci., 18, 284-293 (1998).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Lab HP
Depositor Aruga, Jun (RIKEN Center for Brain Science) Aruga, Jun
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Heterozygous deletion of the linked genes ZIC1 and ZIC4 is involved in Dandy-Walker malformation. Nat. Genet.10: 1053-1055 (2004).(15338008)
Inessa GRINBERG, Hope NORTHRUP, Holly ARDINGER, Chitra PRASAD, William B DOBYNS, Kathleen J MILLEN

RBRC00818, C57BL/6-Tg(Zic1/iresLacZ)1Arg

RBRC No. RBRC00818
Type Transgene CongenicCartagena wks
Species Mus musculus
Strain name C57BL/6-Tg(Zic1/iresLacZ)1Arg
Former Common name Z19K
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description Zic1 is overexpressed in dosal spinalcord. The expression can be monitored by X-gal staining.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome UN
Common name internal ribosomal entry site from EMCV
Symbol description No Data
2 Symbol Zic1
Symbol name zinc finger protein of the cerebellum 1
Chromosome UN
Common name zinc finger protein of the cerebellum 1
Symbol description No Data
3 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome UN
Common name No Data
Symbol description E. coli beta galactosidase
References Dev Biol. 2002 Apr 15;244(2):329-41.

Zic1 promotes the expansion of dorsal neural progenitors in spinal cord by inhibiting neuronal differentiation.
Research applications Analysis of spinal cord development,
Developmental Biology Research,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Dev. Biol., 244, 329-341 (2002).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Lab HP
Depositor Aruga, Jun (RIKEN Center for Brain Science) Aruga, Jun
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC00819, C57BL/6-Tg(Zic1tr/iresLacZ)1Arg

RBRC No. RBRC00819
Type Transgene CongenicCartagena wks
Species Mus musculus
Strain name C57BL/6-Tg(Zic1tr/iresLacZ)1Arg
Former Common name TR9K
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description Dorsal spinal coed is marked by LacZ expression.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome UN
Common name internal ribosomal entry site from EMCV
Symbol description No Data
2 Symbol Zic1
Symbol name zinc finger protein of the cerebellum 1
Chromosome UN
Common name zinc finger protein of the cerebellum 1
Symbol description No Data
3 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome UN
Common name No Data
Symbol description E. coli beta galactosidase
References Dev Biol. 2002 Apr 15;244(2):329-41.

Zic1 promotes the expansion of dorsal neural progenitors in spinal cord by inhibiting neuronal differentiation.
Research applications Analysis of spinal cord development,
Developmental Biology Research,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Dev. Biol., 244, 329-341 (2002).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Lab HP
Depositor Aruga, Jun (RIKEN Center for Brain Science) Aruga, Jun
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC00109, C3.129S4(B6)-Zic1<tm1Jaru>/JaruRbrc

RBRC No. RBRC00109
Type Targeted MutationCartagena
Species Mus musculus
Strain name C3.129S4(B6)-Zic1<tm1Jaru>/JaruRbrc
Former Common name C3H/HeN-TgH(Zic1), Zic1 KO (C3H background)
H-2 Haplotype No Data
ES cell line J1 [129S4/SvJae](D3 subline)
Donor strain B6;129S4-Zic1tm1Jaru
Background strain C3H/HeNJcl
Appearance
1 Appearance agouti
Genotype No Data
Strain development Developed by Dr. Jun Aruga. Protein coding region of Zic1 exon1 is replaced by PGK-neo gene homologous recombination in ES cell. This strain has been introduced into C3H/HeN genetic background by successive backcrossing for more than 10 generations.
Strain description The Zic1 gene is known to have important roles in the cerebellar function. In this strain protein coding region of Zic1 exon 1 was replaced by PGK-Neo gene by homologous recombination in J1 ES cells.
Genotype of Affected Mutation:Zic1 null heterozygous.
Colony maintenance Backcrossing to C3H/HeN. Or sister-brother mating of heterozygotes. Breeding performance is good. The mice are provided as heterozygous breeder pairs.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Zic1
Symbol name zinc finger protein of the cerebellum 1
Chromosome 9
Common name zinc finger protein of the cerebellum 1
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 9
Common name neo; neomycin;
Symbol description No Data
References Brain Res Mol Brain Res. 2000 May 31;78(1-2):15-25.
Dev Biol. 2002 Apr 15;244(2):329-41.
J Neurosci. 1998 Jan 1;18(1):284-93.
Mech Dev. 1999 Dec;89(1-2):141-50.

A 5′ segment of the mouse Zic1 gene contains a region specific enhancer for dorsal hindbrain and spinal cord.
A 5′ segment of the mouse Zic1 gene contains a region specific enhancer for dorsal hindbrain and spinal cord.
A 5′ segment of the mouse Zic1 gene contains a region specific enhancer for dorsal hindbrain and spinal cord.
A 5′ segment of the mouse Zic1 gene contains a region specific enhancer for dorsal hindbrain and spinal cord.
Research applications Developmental Biology Research,
Mouse Models for Human Disease,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neurosci., 18, 284-293 (1998).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Lab HP
Depositor Aruga, Jun (RIKEN Center for Brain Science) Aruga, Jun
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC00165, B6.129S4-Zic2<tm1Jaru>/JaruRbrc

RBRC No. RBRC00165
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129S4-Zic2<tm1Jaru>/JaruRbrc
Former Common name C57BL/6N-Kd(Zic2), Zic2 Knockdown mouse
H-2 Haplotype No Data
Donor strain 129S4/SvJae via J1 ES cell line
Background strain C57BL/6N
Appearance
1 Appearance black
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Zic2
Symbol name zinc finger protein of the cerebellum 2
Chromosome 14
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 14
Common name neo; neomycin;
Symbol description No Data
References J Neurosci. 2002 Jan 1;22(1):218-25.
Proc Natl Acad Sci U S A. 2000 Feb 15;97(4):1618-23.

Research applications Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Proc. Natl. Acad. Sci. USA, 97, 1618-1623 (2000).
Additional information
1 Lab HP
Depositor Aruga, Jun (RIKEN Center for Brain Science) Aruga, Jun
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Zic2 promotes axonal divergence at the optic chiasm midline by EphB1-dependent and -independent mechanisms. Development.135(10): 1833-41 (2008).(18417618)
Garc a-Frigola C, Carreres MI, Vegar C, Mason C, Herrera E.
Zic2 regulates the expression of Sert to modulate eye-specific refinement at the visual targets. EMBO J.29(18): 3170-83 (2010).(20676059)
Garc a-Frigola C, Herrera E.
Shh/Boc signaling is required for sustained generation of ipsilateral projecting ganglion cells in the mouse retina. J Neurosci.33(20): 8596-607 (2013).(23678105)
S nchez-Arrones L, Nieto-Lopez F, S nchez-Camacho C, Carreres MI, Herrera E, Okada A, Bovolenta P.