RBRC00986, B6C3F1-Tg(CAG-EGFP)CX-FM50Osb

RBRC No. RBRC00986
Type TransgeneCartagena wks
Species Mus musculus
Strain name B6C3F1-Tg(CAG-EGFP)CX-FM50Osb
Former Common name B6C3F1B6C3F1 TgN(act-EGFP)OsbCX-FM050
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Masaru Okabe at Osaka University in 1997. The CAG-GFP plasmids were injected into the pronuclei of B6C3F2 fertilized eggs.
Strain description Both homozygous and hemizygous transgenic mice express GFP in all over the body. The transgene is located in X chromosome. This strain is useful for detection of cell fate in transplantation and developmental studies.
Colony maintenance Sibling Mating (Homozygote x Hemizygote)
Health Report
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome X
Common name No Data
Symbol description No Data
References FEBS Lett. 1997 May 5;407(3):313-9.
Genomics. 2002 Dec;80(6):564-74.
Proc Natl Acad Sci U S A 2005 Mar 15;102(11):4039-44. Epub 2005 Mar 3.

‘Green mice’ as a source of ubiquitous green cells.
‘Green mice’ as a source of ubiquitous green cells.
‘Green mice’ as a source of ubiquitous green cells.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genomics, 80, 564-574 (2002). Proc Natl Acad Sci USA.102(11), 4039-4044 (2005).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
2 Genotyping protocol <PCR>
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01990, B6C3F1-Tg(CAG-EGFP)C5-FM053Osb

RBRC No. RBRC01990
Type TransgeneCartagena
Species Mus musculus
Strain name B6C3F1-Tg(CAG-EGFP)C5-FM053Osb
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 5
Common name No Data
Symbol description No Data
References Genomics. 2002 Dec;80(6):564-74.

FISH analysis of 142 EGFP transgene integration sites into the mouse genome.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genomics. 80(6):564-574 (2002).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02006, B6C3F1-Tg(CAG-EGFP)C16-FM071Osb

RBRC No. RBRC02006
Type TransgeneCartagena
Species Mus musculus
Strain name B6C3F1-Tg(CAG-EGFP)C16-FM071Osb
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 16
Common name No Data
Symbol description No Data
References Genomics. 2002 Dec;80(6):564-74.

FISH analysis of 142 EGFP transgene integration sites into the mouse genome.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genomics. 80(6):564-574 (2002).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02022, B6C3F1-Tg(CAG-EGFP)C2-FM120Osb

RBRC No. RBRC02022
Type TransgeneCartagena
Species Mus musculus
Strain name B6C3F1-Tg(CAG-EGFP)C2-FM120Osb
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 2
Common name No Data
Symbol description No Data
References Genomics. 2002 Dec;80(6):564-74.

FISH analysis of 142 EGFP transgene integration sites into the mouse genome.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genomics. 80(6):564-574 (2002).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02038, B6D2F1-Tg(CAG-EGFP)C5-FM170Osb

RBRC No. RBRC02038
Type TransgeneCartagena
Species Mus musculus
Strain name B6D2F1-Tg(CAG-EGFP)C5-FM170Osb
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 5
Common name No Data
Symbol description No Data
References Genomics. 2002 Dec;80(6):564-74.

FISH analysis of 142 EGFP transgene integration sites into the mouse genome.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genomics. 80(6):564-574 (2002).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02054, B6D2F1-Tg(CAG-EGFP-lox71)FM226Osb

RBRC No. RBRC02054
Type TransgeneCartagena
Species Mus musculus
Strain name B6D2F1-Tg(CAG-EGFP-lox71)FM226Osb
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol lox71
Symbol name mutant lox sequence
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
2 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome No Data
Common name No Data
Symbol description No Data
References Genomics. 2002 Dec;80(6):564-74.

FISH analysis of 142 EGFP transgene integration sites into the mouse genome.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genomics. 80(6):564-574 (2002).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02070, B6D2F1-Tg(CAG-EGFP-lox71)C12-FM248Osb

RBRC No. RBRC02070
Type TransgeneCartagena
Species Mus musculus
Strain name B6D2F1-Tg(CAG-EGFP-lox71)C12-FM248Osb
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol lox71
Symbol name mutant lox sequence
Chromosome 12
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
2 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 12
Common name No Data
Symbol description No Data
References Genomics. 2002 Dec;80(6):564-74.

FISH analysis of 142 EGFP transgene integration sites into the mouse genome.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genomics. 80(6):564-574 (2002).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02182, B6.Cg-Tg(Prm1-Cre)01Osb

RBRC No. RBRC02182
Type Transgene CongenicCartagena wks
Species Mus musculus
Strain name B6.Cg-Tg(Prm1-Cre)01Osb
Former Common name protamine-cre
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Masato Ikawa at Osaka University in 1997.
Strain description This transgenic mouse expresses the cre-recombinase under the control of the protamine 1 gene promoter. This strain can be used to make an inducible knock-out by crossing with the other genetically-engineered mice that have floxed genes.
Colony maintenance Backcross to C57BL/6 (Hemizygote x C57BL6/CrSlc)
Health Report
Gene Details
Promoter protamine 1
1 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References Biochem Biophys Res Commun. 2004 Aug 20;321(2):275-9.

Lineage-specific cell disruption in living mice by Cre-mediated expression of diphtheria toxin A chain.
Research applications Cre/loxP system,
Reproductive Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Biochem. Biophs. Res. Commun., 321, 275-279 (2004).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the BIOLOGICAL RESOURCES. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 Lab HP
2 Genetic Background
Breeding characters
3 Genotyping protocol <PCR>
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05860, B6;129-Hhex<tm1Ngu>

RBRC No. RBRC05860
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129-Hhex<tm1Ngu>
Former Common name Hhex KO, B6-Hhex KO
H-2 Haplotype No Data
ES cell line D3 [129S2/SvPas]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Tamio Noguchi, Graduate School of Bioagricultural Sciences, Nagoya University in 2000.
Strain description Hhex gene knockout mice. Homozygous mutant mice are embryonic lethal.
Colony maintenance Heterozygote x Wild-type [C57BL/6NJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Hhex
Symbol name hematopoietically expressed homeobox
Chromosome 19
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 19
Common name neo; neomycin;
Symbol description No Data
References

Research applications Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Biochem. Biophys. Res. Commun., 276, 1155-1161(2000).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 Lab HP
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01347, B6.Cg-Tg(CAG-floxed neo-DT)03Osb

RBRC No. RBRC01347
Type TransgeneCartagena wks
Species Mus musculus
Strain name B6.Cg-Tg(CAG-floxed neo-DT)03Osb
Former Common name REP-DT
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Masaru Okabe, Genome Information Research Center, Osaka University in 2003. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. C57BL/6 and DBA/2 mixed background.
Strain description CAG-DT conditional transgenic mice (REP-DT mice). The transgene consists of the CAG promoter and the diphtheria toxin A chain (DT) cDNA separated by a floxed neo cassette. When the floxed neo cassette is excised by crossing with Cre mice, DT is expressed. It has been reported that healthy but sterile mice due to a disruption of germ line cells are generated when the REP-DT mice were mated with testis specific cre mice (Prm1-cre). Other tissue/organ-specific expression patterns of the transgene are not clear (Caution: The gene expression pattern in transgenic mice can be different from it of the endogenous gene.)
Colony maintenance Carrier x Carrier, Noncarrier [or Crossing to C57BL/6NCrSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol DT
Symbol name diphtheria toxin A (DT-A)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome UN
Common name neo; neomycin;
Symbol description No Data
References Biochem Biophys Res Commun. 2004 Aug 20;321(2):275-9.

Lineage-specific cell disruption in living mice by Cre-mediated expression of diphtheria toxin A chain.
Research applications Cre/loxP system,
General Purpose
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Biochem. Biophys. Res. Commun., 321, 275-279 (2004).
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University (http://www.uic.osaka-u.ac.jp/en/index.html). The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Okabe, Masaru (Osaka university) Okabe, Masaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :