RBRC00457, B6.129P2-Prkcd<tm1Kin>

RBRC No. RBRC00457
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129P2-Prkcd<tm1Kin>
Former Common name PKCδ Knockout mice
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Keiichi Nakayama, Kyusyu University.
Strain description Protein kinase C (PKC) delta is a member of PKC family which phosphorylates a wide variety of protein targets and is known to be involved in diverse cellular signaling pathways. In PKC family members, PKC delta has a unique function to supress cell proliferation. PKC-delta deficient mice exhibit expansion of the B- lymphocyte population and show autoimmune disease after 6 months of age.
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
Health Report
Gene Details
Promoter No Data
1 Symbol Prkcd
Symbol name protein kinase C, delta
Chromosome 14
Common name D14Ertd420e, PKC[d], Pkcd, PKCdelta
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 14
Common name neo; neomycin;
Symbol description No Data
References

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Nakayama, Keiichi (Medical institute of bioregulation, Kyushu University) Nakayama, Keiichi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Genetic disruption of protein kinase C delta reduces endotoxin-induced lung injury. Am J Physiol Lung Cell Mol Physiol.303(10): L880-8 (2012).(22983354)
Chichger H, Grinnell KL, Casserly B, Chung CS, Braza J, Lomas-Neira J, Ayala A, Rounds S, Klinger JR, Harrington EO.

RBRC06400, B6;129P2-Cdkn1b<tm2Kin>

RBRC No. RBRC06400
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Cdkn1b<tm2Kin>
Former Common name p27/S10A KI, B6-Cdkn1b/S10A KI
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Keiichi Nakayama, Medical Institute of Bioregulation, Kyushu University. E14 ES cells were used. C57BL/6J and 129 mixed.
Strain description p23 S10A variant knockin mice (S10A type).
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Cdkn1b
Symbol name cyclin-dependent kinase inhibitor 1B
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
References

Research applications Cancer Research,
Cell Biology Research,
Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
Depositor Nakayama, Keiichi (Medical institute of bioregulation, Kyushu University) Nakayama, Keiichi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC00458, B6.129P2-Cdkn1b<tm1Kin>

RBRC No. RBRC00458
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Cdkn1b<tm1Kin>
Former Common name B6.129P2-Cdkn1b; p27 Knockout mice
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Keiko Nakayama and Kei-ichi Nakayama, Howard Hughes Medical Institute, Washington University School of Medicine in 1996. E14 ES cells were used to generate the mutant mice. The mutant mice were backcrossed to C57BL/6.
Strain description p27 (Cdkn1b) gene knockout mice. A 2.5 kb region containing exons 1 and 2 was replaced with a PGK-neo cassette.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Cdkn1b
Symbol name cyclin-dependent kinase inhibitor 1B
Chromosome 6
Common name Kip1, p27, p27Kip1
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name neo; neomycin;
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Nakayama, Keiichi (Medical institute of bioregulation, Kyushu University) Nakayama, Keiichi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06401, B6;129P2-Cdkn1b<tm3Kin>

RBRC No. RBRC06401
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Cdkn1b<tm3Kin>
Former Common name p27/S10D KI, B6-Cdkn1b/S10D KI
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Keiichi Nakayama, Medical Institute of Bioregulation, Kyushu University. E14 ES cells were used. C57BL/6J and 129 mixed.
Strain description p23 S10D variant knockin mice (S10D type).
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Cdkn1b
Symbol name cyclin-dependent kinase inhibitor 1B
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome No Data
Common name No Data
Symbol description No Data
References No Data

Research applications Cancer Research,
Cell Biology Research,
Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
Depositor Nakayama, Keiichi (Medical institute of bioregulation, Kyushu University) Nakayama, Keiichi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC05808, B6.129P2-Aldh2<tm1Kaw>

RBRC No. RBRC05808
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Aldh2<tm1Kaw>
Former Common name Aldh2KO
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Kyoko Kitagawa and Toshihiro Kawamoto, University of Occupational and Environmental Health in 1998. E14 ES cells were used. The mutant mice were backcrossed to C57BL/6 over 7 times.
Strain description Aldh2 gene knockout mice. A termination codon and a neomycin selection cassette was inserted into exon 3 of Aldh2 gene.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl] Homozygous mutant mice are viable and fertile.
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 Poly A signal
Chromosome 5
Common name No Data
Symbol description No Data
2 Symbol Aldh2
Symbol name aldehyde dehydrogenase 2, mitochondrial
Chromosome 5
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 5
Common name No Data
Symbol description No Data
References


Research applications Metabolism Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Nakayama, Keiichi (Medical institute of bioregulation, Kyushu University) Nakayama, Keiichi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Postinfarction Hearts Are Protected by Premature Senescent Cardiomyocytes Via GATA4-Dependent CCN1 Secretion. J. Am. Heart Assoc.7: e009111. (2018).(30371213)
Sumei Cui, Li Xue, Feihong Yang, Shuai Dai, Ziqi Han, Kai Liu, Baoshan Liu, Qiuhuan Yuan, Zhaoqiang Cui, Yun Zhang, Feng Xu, Yuguo Chen.

RBRC06402, B6;129P2-Cdkn1c<tm2.1Kei>

RBRC No. RBRC06402
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Cdkn1c<tm2.1Kei>
Former Common name p57 cKO, B6-Cdkn1c floxed
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Keiichi Nakayama, Medical Institute of Bioregulation, Kyushu University. E14 ES cells were used. The mutant mice were crossed to C57BL/6J (N6 generations).
Strain description p57 floxed mice. Exons 4 to 4 were flanked by loxP sites (Flox type). Paternal imprinting gene.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Cdkn1c
Symbol name cyclin-dependent kinase inhibitor 1C (P57)
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
References

Research applications Cancer Research,
Cell Biology Research,
Cre/loxP system,
Metabolism Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Nakayama, Keiichi (Medical institute of bioregulation, Kyushu University) Nakayama, Keiichi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06387, B6;129P2-Ube4b<tm1Kei>

RBRC No. RBRC06387
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Ube4b<tm1Kei>
Former Common name E4b KO, B6-Ube4b KO
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Keiichi Nakayama, Medical Institute of Bioregulation, Kyushu University. E14 ES cells were used. The mutant mice were crossed to C57BL/6J over 6 times.
Strain description Ube4b (Ufd2a) gene knockout mice. Exons 23 to 27 were replaced with a PGK-neo (loxP-neo) cassette.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 polyA signal
Chromosome 4
Common name No Data
Symbol description No Data
2 Symbol Ube4b
Symbol name ubiquitination factor E4B
Chromosome 4
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 4
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 4
Common name No Data
Symbol description No Data
References

Research applications Developmental Biology Research,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
Depositor Nakayama, Keiichi (Medical institute of bioregulation, Kyushu University) Nakayama, Keiichi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC06403, B6;129P2-Cdkn1c<tm1Kat>

RBRC No. RBRC06403
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Cdkn1c<tm1Kat>
Former Common name p57 KO, B6-Cdkn1c KO
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Keiichi Nakayama, Medical Institute of Bioregulation, Kyushu University. E14 ES cells were used. The mutant mice were crossed to C57BL/6J.
Strain description p57 gene knockout mice. Exons 1 to 3 and a part of exon 4 were replaced with a PGK-neo cassette. Paternal imprinting gene.
Colony maintenance Heterozygote (male) x Wild-type (female) [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 polyA signal
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol Cdkn1c
Symbol name cyclin-dependent kinase inhibitor 1C (P57)
Chromosome 7
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 7
Common name No Data
Symbol description No Data
References

Research applications Cell Biology Research,
Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
Depositor Nakayama, Keiichi (Medical institute of bioregulation, Kyushu University) Nakayama, Keiichi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC06388, B6;129P2-Fbxl21<tm1Kei>

RBRC No. RBRC06388
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Fbxl21<tm1Kei>
Former Common name Fbxl21 KO, B6-bxl21 KO
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Keiichi Nakayama, Medical Institute of Bioregulation, Kyushu University. E14 ES cells were used. The mutant mice were crossed to C57BL/6J (N7 generations).
Strain description Fbxl21 gene knockout mice. Exons 4 and 5 were replaced with an IRES-lacZ/neo cassette.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 polyA signal
Chromosome 13
Common name No Data
Symbol description No Data
2 Symbol Fbxl21
Symbol name F-box and leucine-rich repeat protein 21
Chromosome 13
Common name No Data
Symbol description No Data
3 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 13
Common name No Data
Symbol description No Data
4 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 13
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 13
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
6 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 13
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System,
Metabolism Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
Depositor Nakayama, Keiichi (Medical institute of bioregulation, Kyushu University) Nakayama, Keiichi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC06404, B6;129P2-Cdkn1c<tm1.1(Cdkn1b*)Kei>

RBRC No. RBRC06404
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Cdkn1c<tm1.1(Cdkn1b*)Kei>
Former Common name p57/p27KI, B6-Cdkn1c/Cdkn1b KI
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Keiichi Nakayama, Medical Institute of Bioregulation, Kyushu University. E14 ES cells were used. The mutant mice were crossed to C57BL/6J over 5 times.
Strain description p57 gene p27 knockin mice. Intron 2 of p57 was replaced with a p27 cDNA (p57<p27KI> type). Paternal imprinting gene.
Colony maintenance Heterozygote (male) x Wild-type (female) [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Cdkn1c
Symbol name cyclin-dependent kinase inhibitor 1C (P57)
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
References

Research applications Cell Biology Research,
Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Nakayama, Keiichi (Medical institute of bioregulation, Kyushu University) Nakayama, Keiichi
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months