RBRC01659, C.FVB-Tg(Itgax-DTR/EGFP)57Lan/Rbrc

RBRC No. RBRC01659
Type TransgeneCartagena
Species Mus musculus
Strain name C.FVB-Tg(Itgax-DTR/EGFP)57Lan/Rbrc
Former Common name No Data
H-2 Haplotype No Data
Background strain BALB/cAnNCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Itgax promoter
2 Symbol HBEGF
Symbol name heparin-binding epidermal growth factor-like growth factor
Chromosome UN
Common name No Data
Symbol description No Data
References Immunity. 2002 Aug;17(2):211-20.

In vivo depletion of CD11c(+) dendritic cells abrogates priming of CD8(+) T cells by exogenous cell-associated antigens.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Research Purpose only. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEVELOPER (Dr. Dan Littman).
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05852, C.B6-a/a B/B Kit<W-sh>

RBRC No. RBRC05852
Type Spontaneous Mutation
Species Mus musculus
Strain name C.B6-a/a B/B Kit<W-sh>
Former Common name BALB-W-sh
H-2 Haplotype No Data
Background strain BALB/cAnNTac
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Shigeo Koyasu, Keio University School of Medicine. The mutant mice were backcrossed to BALB/c for 12 times.
Strain description W-sh BALB/c congenic strain. Homozygous mutant mice are viable and fertile.
Colony maintenance Heterozygote x Homozygote [or Crossing to BALB/cAnNTac]
Health Report
Gene Details
Promoter No Data
1 Symbol Kit
Symbol name kit oncogene
Chromosome 5
Common name No Data
Symbol description No Data
References No Data

Research applications Immunology and Inflammation Research
Specific Term and Conditions No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC01660, C57BL/6N-Tg(AK7-H18)/Rbrc

RBRC No. RBRC01660
Type TransgeneCartagena wks
Species Mus musculus
Strain name C57BL/6N-Tg(AK7-H18)/Rbrc
Former Common name C57BL/6-AK7-H18 mice
H-2 Haplotype No Data
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol AK7-H18
Symbol name No Data
Chromosome No Data
Common name No Data
Symbol description No Data
References Int Immunol. 2004 Oct;16(10):1487-95. Epub 2004 Aug 31.

Auto-reactive B cells against peripheral antigen, desmoglein 3, escape from tolerance mechanism.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Int. Immunol., 16, 1487-1495 (2004).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05853, B6.Cg-Igh<tm1(AK23)>

RBRC No. RBRC05853
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Igh<tm1(AK23)>
Former Common name AK23 IgH KI, B6-IgH KI
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Shigeo Koyasu, Keio University School of Medicine.
Strain description The endogenous DQ53 and JH1-4 elements were replaced by a anti-Dsg3 VDJ (AK23) H chain.
Colony maintenance Heterozygote x Wild-type [C57BL/6NTac]
Health Report
Gene Details
Promoter No Data
1 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 12
Common name neo; neomycin;
Symbol description No Data
2 Symbol Igh
Symbol name immunoglobulin heavy chain complex
Chromosome 12
Common name No Data
Symbol description No Data
References No Data

Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must include of the DEVELOPER as a co-author of any paper before the DEVELOPER publishes the original paper describing the BIOLOGICAL RESOURCE. After publication of the original paper, the RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. but may not include the DEVELOPER as an author.
Additional information
1 Lab HP
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01719, C57BL/6N-Tg(AK7-LH1)/Rbrc

RBRC No. RBRC01719
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6N-Tg(AK7-LH1)/Rbrc
Former Common name Ig(H+L) Tg(AK7-LH1) mice
H-2 Haplotype No Data
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter κ chain promoter
1 Symbol AK7-L
Symbol name No Data
Chromosome UN
Common name No Data
Symbol description No Data
Promoter H chain promoter
2 Symbol AK7-H
Symbol name No Data
Chromosome UN
Common name No Data
Symbol description No Data
References Int Immunol. 2004 Oct;16(10):1487-95. Epub 2004 Aug 31.

Auto-reactive B cells against peripheral antigen, desmoglein 3, escape from tolerance mechanism.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Int. Immunol., 16, 1487-1495 (2004).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05854, B6.Cg-Tg(IgkAK23)

RBRC No. RBRC05854
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(IgkAK23)
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Shigeo Koyasu, Keio University School of Medicine. C57BL/6 and 129 mixed background.
Strain description Ant-Dsg3 Ig (AK23) L chain transgenic mice.
Colony maintenance Carrier x Noncarrier [C57BL/6NTac]
Health Report
Gene Details
Promoter No Data
1 Symbol Igk
Symbol name immunoglobulin kappa chain complex
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must include of the DEVELOPER as a co-author of any paper before the DEVELOPER publishes the original paper describing the BIOLOGICAL RESOURCE. After publication of the original paper, the RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. but may not include the DEVELOPER as an author.
Additional information
1 Lab HP
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01720, B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/Rbrc

RBRC No. RBRC01720
Type TransgeneCartagena wks
Species Mus musculus
Strain name B6.FVB-Tg(Itgax-DTR/EGFP)57Lan/Rbrc
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Itgax promoter
2 Symbol HBEGF
Symbol name heparin-binding EGF-like growth factor (monkey)
Chromosome UN
Common name No Data
Symbol description No Data
References Immunity. 2002 Aug;17(2):211-20.

In vivo depletion of CD11c(+) dendritic cells abrogates priming of CD8(+) T cells by exogenous cell-associated antigens.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Immunity, 17, 211-220 (2002).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Research Purpose only. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEVELOPER (Dr. Dan Littman).
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05893, B6.Cg-Tg(AKT/Ert2,EGFP)1Nkn

RBRC No. RBRC05893
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(AKT/Ert2,EGFP)1Nkn
Former Common name Akt-Mer Tg, B6-AKT/Ert2, GFP Tg
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Toru Nakano, Graduate School of Medicine, Osaka University. The mice were backcrossed to C57BL/6N over 8 times by Shigeo Koyasu, School of Medicine, Keio University.
Strain description AKT-Mer transgenic mice. These transgenic mice express a fusion protein composed of a myristoylated constitutively active form of human Akt and the ligand-binding domain of a mutant estrogen receptor under the control of the CAG promoter. The Akt-Mer was also fused to IRES-EGFP, can be monitored Akt-Mer expression by GFP fluorescence.
Colony maintenance Carrier x Noncarrier [C57BL/6NCrSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol ERT2
Symbol name estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol HA
Symbol name human influenza virus HA (hemagglutinin) tag sequence
Chromosome UN
Common name No Data
Symbol description No Data
4 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
5 Symbol AKT
Symbol name thymoma viral proto-oncogene (human)
Chromosome UN
Common name No Data
Symbol description No Data
References Oncogene. 2007 Feb 12;.

Akt activation induces epidermal hyperplasia and proliferation of epidermal progenitors.
Akt activation induces epidermal hyperplasia and proliferation of epidermal progenitors.
Research applications Cancer Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER, Professor Toru Nakano of Osaka University.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01721, Bcl-xL Tg

RBRC No. RBRC01721
Type TransgeneCartagena wks
Species Mus musculus
Strain name Bcl-xL Tg
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Gabriel Nunez at University of Michigan Medical School in 1995. The human Bcl-xL under the control of the SV40 promoter and IgH enhancer (SV40-EH casette) was injected into the pronuclei of (C57BL/6 x SJL/J)F2 fertilized egges.
Strain description C57BL/6-Tg(SV40-BCL2L1)87Nnz. Bcl-xL, a member of the bcl-2 amily, is expressed in pre-B cells but downregulated at the immature and mature stages of B cell development. This transgenic mouse strain expresses the human Bcl-xL gene within B cell lineage under the control of the SV40 promoter and B IgH enhancer. The transgenic mice show marked accumulation of peripheral B cells in lymphoid organs and enhanced B cell survival in vitro.
Colony maintenance Backcross to C57BL/6 (Hemizygote x C57BL/6NCrj).
Health Report No Data
Gene Details
Promoter No Data
1 Symbol poly A
Symbol name SV40 poly adenylation signal sequence
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Immunogloblin Eu enhancer, SV 40 early promoter, SV40 poly A additional signal
2 Symbol BCL2L1
Symbol name Bcl2-like 1
Chromosome UN
Common name bcl-x, Bcl-XL, Bcl-Xs, BclX
Symbol description No Data
References J Exp Med. 1996 Feb 1;183(2):381-91.

bcl-x exhibits regulated expression during B cell development and activation and modulates lymphocyte survival in transgenic mice.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Exp. Med., 183, 381-391 (1991).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Research Purpose only. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEVELOPER (Dr. Timothy W. Behens).
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01730, C.129S2-Ptprc<tm1Tyb>/Rbrc

RBRC No. RBRC01730
Type Targeted MutationCartagena
Species Mus musculus
Strain name C.129S2-Ptprc<tm1Tyb>/Rbrc
Former Common name CD45 knockout mouse
H-2 Haplotype No Data
Donor strain 129S2/SvPas via D2 ES cell line
Background strain BALB/cAnNCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Victor L J Tybulewicz at National Institute for Medical Research in 1999. A neo was transfered into D2 ES cells to reolace the exon 12 of the Ptprc gene. BALB/c background.
Strain description CD45 is a transmembrane tyrosine phosphatase implicated in the control of T cell receptor signaling. CD45 homozygous mutant mice in the BALB/c background were gorssly normal and fertile.
Colony maintenance Backcross to BALB/c (Heterozygote x BALB/cAnN)
Health Report
Gene Details
Promoter No Data
1 Symbol Ptprc
Symbol name protein tyrosine phosphatase, receptor type, C
Chromosome 1
Common name B220, CD45, Cd45, CD45R, Ly-5, Lyt-4, T200
Symbol description No Data
2 Symbol poly A
Symbol name HSV TK poly A
Chromosome 1
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 1
Common name neo; neomycin;
Symbol description No Data
References Eur J Immunol. 1999 Sep;29(9):2923-33.

Greatly reduced efficiency of both positive and negative selection of thymocytes in CD45 tyrosine phosphatase-deficient mice.
Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Eur. immunol., 29, 2923-2933 (1999).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Research Purpose only. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEVELOPER (Dr. Victor Tybulewicz).
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months