RBRC00850, B6.129P2-Itgal tm1Mak

RBRC No. RBRC00850
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6.129P2-Itgaltm1Mak
Former Common name C57BL/6-[KO]LFA-1; B6.129P2-Itgb2tm1Oci
H-2 Haplotype No Data
Donor strain 129P2/OlaHsd via E14 ES cell line
Background strain No Data
Appearance
1 Appearance black
Genotype No Data
Strain development From Tak. W. Mak of ontario Cancer Institute, N6
Strain description Original line was established by Tak Mak at Ontario Cancer Institute in Tronto (Schmits et al., J. Exp. Med., 183, 1415, 1996). Neo registance gene construct was introduced into the CD11a allele of an ES cell line E14k of 129/Ola origin. Mutant ES cells were injected to C57BL/6 blastocysts. Chimeras were mated with C57BL/6 and was backcrossed to C57BL/6 for 6 generations. After embryo transfer at Taconic, the colony has been maintained through intercrossing of homozygous mice.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Itgal
Symbol name integrin alpha L
Chromosome 7
Common name CD11a, Integrin alpha L
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 7
Common name neo; neomycin;
Symbol description No Data
References J Exp Med. 1996 Apr 1;183(4):1415-26.

LFA-1-deficient mice show normal CTL responses to virus but fail to reject immunogenic tumor.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
TLR7 mediated viral recognition results in focal type I interferon secretion by dendritic cells. Nat Commun8(1): 1592 (2017).(29150602)
Saitoh SI, Abe F, Kanno A, Tanimura N, Mori Saitoh Y, Fukui R, Shibata T, Sato K, Ichinohe T, Hayashi M, Kubota K, Kozuka-Hata H, Oyama M, Kikko Y, Katada T, Kontani K, Miyake K.

RBRC02304, B6.Cg-Kit<W-sh>(N18)

RBRC No. RBRC02304
Type Spontaneous Mutation Congenic wks
Species Mus musculus
Strain name B6.Cg-Kit<W-sh>(N18)
Former Common name C57BL6-W N18
H-2 Haplotype No Data
Background strain C57BL/6NTac
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Kit
Symbol name kit oncogene
Chromosome 5
Common name belly-spot, c-KIT, CD117, Dominant white spotting, Steel Factor Receptor, Tr-kit
Symbol description No Data
References Am J Pathol. 2005 Sep;167(3):835-48.
Development. 1993 Jul;118(3):705-17.
Genet Res. 1982 Jun;39(3):315-22.

Mast cell-deficient W-sash c-kit mutant Kit W-sh/W-sh mice as a model for investigating mast cell biology in vivo.
Mast cell-deficient W-sash c-kit mutant Kit W-sh/W-sh mice as a model for investigating mast cell biology in vivo.
Mast cell-deficient W-sash c-kit mutant Kit W-sh/W-sh mice as a model for investigating mast cell biology in vivo.
Research applications Dermatology Research,
Developmental Biology Research,
Immunology and Inflammation Research,
Reproductive Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT agrees to use of BIOLOGICAL RESOURCE only for publishing the research papers. In publishing research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowlidgement to the DEPOSITOR is requested.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC01577, C.Cg-Pik3r1<tm1Tka>/Rbrc

RBRC No. RBRC01577
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name C.Cg-Pik3r1<tm1Tka>/Rbrc
Former Common name BALB/c-PI3K KO
H-2 Haplotype No Data
Donor strain (C57BL/6 x CBA)F1 via TT2 ES cell line
Background strain BALB/cAnNCrlCrlj
Appearance
1 Appearance albino
Genotype A/A b/b c/c
Strain development Developed by Dr. Kadowaki of the University of Tokyo and backcrossed to BALB/c for 12 generations by depositor.
Strain description C.Cg-Pik3r1<tm1Tka>. The PI3K, phosphoinositide-3-kinase gene is a key enzyme producing phospholipid second messengers and plays an important role in various signal transduction pathways. This knockout mouse strain is useful for investigating the function of the PI3K gene.
Colony maintenance Since most of homozygous females are sterile, we maintain the colony by mating heterozygous females with homozygous males.
Health Report
Gene Details
Promoter No Data
1 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 13
Common name neo; neomycin;
Symbol description No Data
2 Symbol Pik3r1
Symbol name phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
Chromosome 13
Common name p50alpha, p55alpha, p85alpha, PI3K
Symbol description No Data
3 Symbol poly A
Symbol name HSV TK poly A
Chromosome 13
Common name No Data
Symbol description No Data
References Nat Genet. 1999 Feb;21(2):230-5.
Science. 1999 Jan 15;283(5400):390-2.

Increased insulin sensitivity and hypoglycaemia in mice lacking the p85 alpha subunit of phosphoinositide 3-kinase.
Increased insulin sensitivity and hypoglycaemia in mice lacking the p85 alpha subunit of phosphoinositide 3-kinase.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Science, 283, 390-392 (1999). Nat. genet., 21, 230-235 (1999).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Role of phosphatidylinositol 3-kinase in friend spleen focus-forming virus-induced erythroid disease. J Virol.84(15): 7675-82 (2010).(20504929)
Umehara D, Watanabe S, Ochi H, Anai Y, Ahmed N, Kannagi M, Hanson C, Ruscetti S, Nishigaki K.

RBRC04047, B6.129S6-Nfkbid<tm1Clay>

RBRC No. RBRC04047
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129S6-Nfkbid<tm1Clay>
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name No Data
Chromosome No Data
Common name No Data
Symbol description No Data
References J Immunol. 2007 Aug 1;179(3):1681-92.

Functional Role for I{kappa}BNS in T Cell Cytokine Regulation As Revealed by Targeted Gene Disruption.
Functional Role for I{kappa}BNS in T Cell Cytokine Regulation As Revealed by Targeted Gene Disruption.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement with Dr. Linda K. Clayton, Dana-Farber Cancer Institute (DFCI)).
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01578, B6.Cg-Pik3r1<tm1Tka>/Rbrc

RBRC No. RBRC01578
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Pik3r1<tm1Tka>/Rbrc
Former Common name C57BL/6-PI3K KO
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Donor strain (C57BL/6 x CBA)F1 via TT2 ES cell line
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Kadowaki of the University of Tokyo and backcrossed to C57BL/6 for 12 generations by depositor.
Strain description PI3K, phosphoinositide-3-kinase gene is a lipid kinases that catalyze the addition of a phosphate molecule specifically to the 3-position of the inositol ring of phosphoinositides. This strain is useful for elucidating of the rule of PI3K in various phases of immune responses. Homozygous mutant females were sterile.
Colony maintenance Since most of homozygous females are sterile, we maintain the colony by mating heterozygous females with homozygous males.
Health Report
Gene Details
Promoter No Data
1 Symbol Pik3r1
Symbol name phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha)
Chromosome 13
Common name p50alpha, p55alpha, p85alpha, PI3K
Symbol description No Data
Promoter herpes simplex virus thymidine kinase (HSV TK) poly A
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 13
Common name neo; neomycin;
Symbol description No Data
References Nat Genet. 1999 Feb;21(2):230-5.
Science. 1999 Jan 15;283(5400):390-2.

Increased insulin sensitivity and hypoglycaemia in mice lacking the p85 alpha subunit of phosphoinositide 3-kinase.
Increased insulin sensitivity and hypoglycaemia in mice lacking the p85 alpha subunit of phosphoinositide 3-kinase.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Science, 283, 390-392 (1999). Nat. genet., 21, 230-235 (1999).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04048, C.129S6(B6)-Nfkbid<tm1Clay>

RBRC No. RBRC04048
Type Targeted MutationCartagena
Species Mus musculus
Strain name C.129S6(B6)-Nfkbid<tm1Clay>
Former Common name BALB/c-IkBNS knockout mice
H-2 Haplotype No Data
Background strain BALB/c
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name No Data
Chromosome No Data
Common name No Data
Symbol description No Data
References J Immunol. 2007 Aug 1;179(3):1681-92.

Functional Role for I{kappa}BNS in T Cell Cytokine Regulation As Revealed by Targeted Gene Disruption.
Functional Role for I{kappa}BNS in T Cell Cytokine Regulation As Revealed by Targeted Gene Disruption.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement with Dr. Linda K. Clayton, Dana-Farber Cancer Institute (DFCI)).
Additional information
1 Lab HP
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC01579, C57BL/6-W<sh>

RBRC No. RBRC01579
Type Spontaneous Mutation
Species Mus musculus
Strain name C57BL/6-W<sh>
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Kit
Symbol name kit oncogene
Chromosome 5
Common name No Data
Symbol description No Data
References Am J Pathol. 2005 Sep;167(3):835-48.
Blood. 1992 Sep 15;80(6):1448-53.
Blood. 1994 Jun 15;83(12):3509-16.
Blood. 1999 Oct 15;94(8):2658-66.
Development. 1993 Jul;118(3):705-17.
Genet Res. 1982 Jun;39(3):315-22.

Mast cell-deficient W-sash c-kit mutant Kit W-sh/W-sh mice as a model for investigating mast cell biology in vivo.
Mast cell-deficient W-sash c-kit mutant Kit W-sh/W-sh mice as a model for investigating mast cell biology in vivo.
Mast cell-deficient W-sash c-kit mutant Kit W-sh/W-sh mice as a model for investigating mast cell biology in vivo.
Mast cell-deficient W-sash c-kit mutant Kit W-sh/W-sh mice as a model for investigating mast cell biology in vivo.
Mast cell-deficient W-sash c-kit mutant Kit W-sh/W-sh mice as a model for investigating mast cell biology in vivo.
Mast cell-deficient W-sash c-kit mutant Kit W-sh/W-sh mice as a model for investigating mast cell biology in vivo.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genet. Res., 39, 315-322 (1982). Development, 118, 705-717 (1993).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04049, C57BL/6-Tg(Lck-Nfkbid)1Clay

RBRC No. RBRC04049
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(Lck-Nfkbid)1Clay
Former Common name C57BL/6-IkBNS transgenic mice
H-2 Haplotype No Data
Background strain C57BL/6NTac
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter mouse Lck promoter
1 Symbol Nfkbid
Symbol name nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, delta
Chromosome UN
Common name No Data
Symbol description No Data
References Unpublished

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement with Dr. Linda K. Clayton, Dana-Farber Cancer Institute (DFCI)).
Additional information
1 Lab HP
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01659, C.FVB-Tg(Itgax-DTR/EGFP)57Lan/Rbrc

RBRC No. RBRC01659
Type TransgeneCartagena
Species Mus musculus
Strain name C.FVB-Tg(Itgax-DTR/EGFP)57Lan/Rbrc
Former Common name No Data
H-2 Haplotype No Data
Background strain BALB/cAnNCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Itgax promoter
2 Symbol HBEGF
Symbol name heparin-binding epidermal growth factor-like growth factor
Chromosome UN
Common name No Data
Symbol description No Data
References Immunity. 2002 Aug;17(2):211-20.

In vivo depletion of CD11c(+) dendritic cells abrogates priming of CD8(+) T cells by exogenous cell-associated antigens.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Research Purpose only. The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEVELOPER (Dr. Dan Littman).
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05852, C.B6-a/a B/B Kit<W-sh>

RBRC No. RBRC05852
Type Spontaneous Mutation
Species Mus musculus
Strain name C.B6-a/a B/B Kit<W-sh>
Former Common name BALB-W-sh
H-2 Haplotype No Data
Background strain BALB/cAnNTac
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Shigeo Koyasu, Keio University School of Medicine. The mutant mice were backcrossed to BALB/c for 12 times.
Strain description W-sh BALB/c congenic strain. Homozygous mutant mice are viable and fertile.
Colony maintenance Heterozygote x Homozygote [or Crossing to BALB/cAnNTac]
Health Report
Gene Details
Promoter No Data
1 Symbol Kit
Symbol name kit oncogene
Chromosome 5
Common name No Data
Symbol description No Data
References No Data

Research applications Immunology and Inflammation Research
Specific Term and Conditions No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Koyasu, Shigeo (Keio University) Koyasu, Shigeo
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :