RBRC01370, CAG-CAT-Notch2#1

RBRC No. RBRC01370
Type TransgeneCartagena wks
Species Mus musculus
Strain name CAG-CAT-Notch2#1
Former Common name No Data
H-2 Haplotype No Data
Background strain ICR
Appearance
1 Appearance albino
Genotype c/c
Strain development Developed by Dr. Hiroki Kokubo, National Institute of Genetics, Japan in 2004. This strain was maintained under ICR background.
Strain description ICR.Cg-Tg(CAG-CAT-Notch2)1Ysa. Notch1, Notch gene homolog 2 (Drosophila) is important in establishing metameric pattern during somitogenesis. This transgenic mouse expresses the active Notch2 gene after excision of the floxed-CAT gene by Cre-mediated recombination. This strain are useful for elucidation of the Notch-signaling mechanism underlying initial segmentation in the presomitic mesoderm.
Colony maintenance Homozygote x Hemizygote or Crossing to ICR (Jcl:MCH)
Health Report
Gene Details
Promoter No Data
1 Symbol Notch2
Symbol name Notch gene homolog 2 (Drosophila)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
3 Symbol cat
Symbol name E. coli Chloramphenicol acetyltransferase (CAT)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
A permission of the DEPOSITOR is required. Within a period of 2 years after deposition of the material, user must contact the DEPOSITOR and notify him a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then user must include DEPOSITOR and/or developer’s names as co-authors.
Additional information
1 Saga Lab HP
2 Genotyping protocol <PCR>
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01763, ICR.Cg-Tg(Hey1-lacZ)1Kkb/YsaRbrc

RBRC No. RBRC01763
Type TransgeneCartagena
Species Mus musculus
Strain name ICR.Cg-Tg(Hey1-lacZ)1Kkb/YsaRbrc
Former Common name hesr1Bglsac-nlacZ line
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome UN
Common name internal ribosomal entry site from EMCV
Symbol description No Data
2 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter hesr1 promoter
3 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome UN
Common name No Data
Symbol description E. coli beta galactosidase
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
A permission of the DEPOSITOR is required. Within a period of 2 years after deposition of the material, user must contact the DEPOSITOR and notify him a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then user must include DEPOSITOR and/or developer’s names as co-authors.
Additional information
1 Saga Lab HP
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02228, TOP-GAL

RBRC No. RBRC02228
Type TransgeneCartagena
Species Mus musculus
Strain name TOP-GAL
Former Common name No Data
H-2 Haplotype No Data
Donor strain (C57BL/6 x C3H)F2
Background strain ICR
Appearance
1 Appearance albino
Genotype c/c
Strain development Developed by Yumiko Saga, National Institute of Genetics in 2005. The transgene was injected into the pronuclei of (C57BL/6 x C3H)F2 fertilized eggs. ICR mixed background.
Strain description Transgenic mice for visualizing Wnt signal pathway. Wnt proteins belong to a family of secreted and lipid-modified glycoprotein that can activate intracellular signaling pathways, such as the Wnt/planar cell polarity, the Wnt/calcium and the Wnt beta-catenin or so-called Wnt canonical signaling pathways. The TOP-GAL transgenic mice are a reporter line which can visualize the Wnt canonical pathway by beta-galactosidase activity. The transgenic mice were generated with ins-TOPGAL that accurately drive beta-galactosidase under TCF/beta-catenin. These transgenic mice will provide you unique opportunity to investigate the function of Wnt canonical pathway in vivo.
Colony maintenance Carrier x Noncarrier [or Crossing to Jcl:MCH(ICR)]. Tg mice are viable and fertile.
Health Report
Gene Details
Promoter No Data
1 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter TOPFLUSH promoter
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome UN
Common name No Data
Symbol description E. coli beta galactosidase
References Genesis. 2007 Feb;45(2):90-100.

GFP transgenic mice reveal active canonical Wnt signal in neonatal brain and in adult liver and spleen.
Research applications Developmental Biology Research,
Fluorescent Proteins/lacZ System,
General Purpose
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genesis, 45, 90-100 (2007).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Mouse of the Month Aug 2009
Genetic Background
2 Saga Lab HP
3 Genotyping protocol <PCR>
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Investigating Tonic Wnt Signaling Throughout the Adult CNS and in the Hippocampal Neurogenic Niche of BatGal and Ins-TopGal Mice. Cell Mol Neurobiol. (2012).(22491991)
Garbe DS, Ring RH.
beta-CateninC429S mice exhibit sterility consequent to spatiotemporally sustained Wnt signalling in the internal genitalia. Sci Rep.4: 6959 (2014).(25376241)
Murata T, Ishitsuka Y, Karouji K, Kaneda H, Toki H, Nakai Y, Makino S, Fukumura R, Kotaki H, Wakana S, Noda T, Gondo Y.
Differential requirements for beta-catenin in murine prostate cancer originating from basal versus luminal cells. J. Pathol.236(3): 290-301 (2015).(25712462)
Lu TL, Chen CM.
Bcl11b SWI/SNF-complex subunit modulates intestinal adenoma and regeneration after -irradiation through Wnt/ -catenin pathway. Carcinogenesis (2015).(25827435)
Sakamaki A, Katsuragi Y, Otsuka K, Tomita M, Obata M, Iwasaki T, Abe M, Sato T, Ochiai M, Sakuraba Y, Aoyagi Y, Gondo Y, Sakimura K, Nakagama H, Mishima Y, Kominami R.

RBRC02508, Δneo-mNos2-L(N2enh-nos2(dZ)EGFP)#2

RBRC No. RBRC02508
Type Targeted Mutation and TransgeneCartagena wks
Species Mus musculus
Strain name Δneo-mNos2-L(N2enh-nos2(dZ)EGFP)#2
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome UN
Common name No Data
Symbol description E. coli beta galactosidase
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter Nanos2 enhancer promoter
3 Symbol Nanos2
Symbol name nanos homolog 2(Drosophila)
Chromosome UN
Common name nos2
Symbol description No Data
4 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Within a period of two years after deposition of the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and/or the DEVELOPER’s names as co-authors.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Saga Lab HP
2 Genotyping protocol <PCR>
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01142, ICR.Cg-Tg(CAG-cat,-Notch1)1Ysa/YsaRbrc

RBRC No. RBRC01142
Type TransgeneCartagena
Species Mus musculus
Strain name ICR.Cg-Tg(CAG-cat,-Notch1)1Ysa/YsaRbrc
Former Common name CAG-CAT-Notch1
H-2 Haplotype No Data
Background strain Jcl:MCH(ICR)
Appearance
1 Appearance albino
Genotype c/c
Strain development Developed by Dr. Yumiko Saga (2002). The transgene fragment was injected into the pronuclei of B6C3F1 fertilized eggs. This strain was maintained with ICR background.
Strain description Notch1, Notch gene homolog 1 (Drosophila) is imortant in establishing metameric pattern during somitogenesis. This transgenic mouse expresses the active Notch1 gene after mating with a cre transgenic mouse. This strain are useful for elucidation of the Notch-signalling mechanism underlying initial segmentation in the presomitic mesoderm.
Colony maintenance Sibling Mating
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Notch1
Symbol name Notch gene homolog 1 (Drosophila)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
4 Symbol cat
Symbol name E. coli Chloramphenicol acetyltransferase (CAT)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Saga Lab HP
2 Genotyping protocol <PCR>
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Distinct roles for cell-autonomous Notch signaling in cardiomyocytes of the embryonic and adult heart. Circ Res.106(3): 559-72 (2010).(20007915)
Kratsios P, Catela C, Salimova E, Huth M, Berno V, Rosenthal N, Mourkioti F.

RBRC01371, CAG-CAT-Notch2#2

RBRC No. RBRC01371
Type TransgeneCartagena
Species Mus musculus
Strain name CAG-CAT-Notch2#2
Former Common name No Data
H-2 Haplotype No Data
Background strain ICR
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Notch2
Symbol name Notch gene homolog 2 (Drosophila)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
3 Symbol cat
Symbol name E. coli Chloramphenicol acetyltransferase (CAT)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
A permission of the DEPOSITOR is required. Within a period of 2 years after deposition of the material, user must contact the DEPOSITOR and notify him a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then user must include DEPOSITOR and/or developer’s names as co-authors.
Additional information
1 Saga Lab HP
2 Genotyping protocol <PCR>
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC01764, nanos1-B6

RBRC No. RBRC01764
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name nanos1-B6
Former Common name No Data
H-2 Haplotype No Data
Donor strain (C57BL/6 x CBA)F1 via TT2 ES cell line
Background strain C57BL/6
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Nanos1
Symbol name nanos homolog 1 (Drosophila)
Chromosome 19
Common name No Data
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 19
Common name No Data
Symbol description E. coli beta galactosidase
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mech Dev., 120, 721-731 (2003).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Saga Lab HP
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02489, ICR.Cg-Tg(P2APdeltaEME)1Ysa/YsaRbrc

RBRC No. RBRC02489
Type TransgeneCartagena
Species Mus musculus
Strain name ICR.Cg-Tg(P2APdeltaEME)1Ysa/YsaRbrc
Former Common name BAC-P2AP Delta EME#1
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol ALP
Symbol name alkaline phosphatase (human)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome UN
Common name neo; neomycin;
Symbol description No Data
References Mech Dev. 2008 May-Jun;125(5-6):432-40. Epub 2008 Feb 5.

Identification of presomitic mesoderm (PSM)-specific Mesp1 enhancer and generation of a PSM-specific Mesp1/Mesp2-null mouse using BAC-based rescue technology.
Research applications Developmental Biology Research,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mech Dev., 125, 432-440 (2008).
Additional information
1 Saga Lab HP
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02509, Δneo-mNos2-L(N2enh-nos2EGFP)#2

RBRC No. RBRC02509
Type Targeted Mutation and TransgeneCartagena
Species Mus musculus
Strain name Δneo-mNos2-L(N2enh-nos2EGFP)#2
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome UN
Common name No Data
Symbol description E. coli beta galactosidase
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter Nanos2 enhancer promoter
3 Symbol Nanos2
Symbol name nanos homolog 2(Drosophila)
Chromosome UN
Common name nos2
Symbol description No Data
4 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Within a period of two years after deposition of the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and/or the DEVELOPER’s names as co-authors.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Saga Lab HP
2 Genotyping protocol <PCR>
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC01143, CAG-CAT-Delta1

RBRC No. RBRC01143
Type TransgeneCartagena
Species Mus musculus
Strain name CAG-CAT-Delta1
Former Common name No Data
H-2 Haplotype No Data
Background strain ICR MCH
Appearance
1 Appearance albino
Genotype c/c
Strain development Developed by Dr. Yumiko Saga (2002). The transgene fragment was injected into the pronucleous of B6C3F1 fertilized eggs. This strain were maintained under ICR background.
Strain description Dll1, delta-like 1 (Drosophila) is one of Notch ligands. This transgenic mouse expresses the Dll1 gene after mating with a cre transgenic mouse. This strain is useful for elucidation of the Notch-signalling mechanism underlying initial segmentation in the presomitic mesoderm.
Colony maintenance Sibling Mating
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Dll1
Symbol name delta-like 1 (Drosophila)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name myc-tag
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol No Data
Symbol name signal pep
Chromosome UN
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
6 Symbol cat
Symbol name E. coli Chloramphenicol acetyltransferase (CAT)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Saga Lab HP
2 Genotyping protocol <PCR>
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months