RBRC01143, CAG-CAT-Delta1

RBRC No. RBRC01143
Type TransgeneCartagena
Species Mus musculus
Strain name CAG-CAT-Delta1
Former Common name No Data
H-2 Haplotype No Data
Background strain ICR MCH
Appearance
1 Appearance albino
Genotype c/c
Strain development Developed by Dr. Yumiko Saga (2002). The transgene fragment was injected into the pronucleous of B6C3F1 fertilized eggs. This strain were maintained under ICR background.
Strain description Dll1, delta-like 1 (Drosophila) is one of Notch ligands. This transgenic mouse expresses the Dll1 gene after mating with a cre transgenic mouse. This strain is useful for elucidation of the Notch-signalling mechanism underlying initial segmentation in the presomitic mesoderm.
Colony maintenance Sibling Mating
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Dll1
Symbol name delta-like 1 (Drosophila)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name myc-tag
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol No Data
Symbol name signal pep
Chromosome UN
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
6 Symbol cat
Symbol name E. coli Chloramphenicol acetyltransferase (CAT)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Saga Lab HP
2 Genotyping protocol <PCR>
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01372, CAG-CAT-Dll4#1

RBRC No. RBRC01372
Type TransgeneCartagena
Species Mus musculus
Strain name CAG-CAT-Dll4#1
Former Common name No Data
H-2 Haplotype No Data
Background strain ICR
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Dll4
Symbol name Delta4
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
3 Symbol cat
Symbol name E. coli Chloramphenicol acetyltransferase (CAT)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
A permission of the DEPOSITOR is required. Within a period of 2 years after deposition of the material, user must contact the DEPOSITOR and notify him a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then user must include DEPOSITOR and/or developer’s names as co-authors.
Additional information
1 Saga Lab HP
2 Genotyping protocol <PCR>
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01764, nanos1-B6

RBRC No. RBRC01764
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name nanos1-B6
Former Common name No Data
H-2 Haplotype No Data
Donor strain (C57BL/6 x CBA)F1 via TT2 ES cell line
Background strain C57BL/6
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Nanos1
Symbol name nanos homolog 1 (Drosophila)
Chromosome 19
Common name No Data
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 19
Common name No Data
Symbol description E. coli beta galactosidase
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mech Dev., 120, 721-731 (2003).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Saga Lab HP
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02489, ICR.Cg-Tg(P2APdeltaEME)1Ysa/YsaRbrc

RBRC No. RBRC02489
Type TransgeneCartagena
Species Mus musculus
Strain name ICR.Cg-Tg(P2APdeltaEME)1Ysa/YsaRbrc
Former Common name BAC-P2AP Delta EME#1
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol ALP
Symbol name alkaline phosphatase (human)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome UN
Common name neo; neomycin;
Symbol description No Data
References Mech Dev. 2008 May-Jun;125(5-6):432-40. Epub 2008 Feb 5.

Identification of presomitic mesoderm (PSM)-specific Mesp1 enhancer and generation of a PSM-specific Mesp1/Mesp2-null mouse using BAC-based rescue technology.
Research applications Developmental Biology Research,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mech Dev., 125, 432-440 (2008).
Additional information
1 Saga Lab HP
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02509, Δneo-mNos2-L(N2enh-nos2EGFP)#2

RBRC No. RBRC02509
Type Targeted Mutation and TransgeneCartagena
Species Mus musculus
Strain name Δneo-mNos2-L(N2enh-nos2EGFP)#2
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome UN
Common name No Data
Symbol description E. coli beta galactosidase
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter Nanos2 enhancer promoter
3 Symbol Nanos2
Symbol name nanos homolog 2(Drosophila)
Chromosome UN
Common name nos2
Symbol description No Data
4 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Within a period of two years after deposition of the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and/or the DEVELOPER’s names as co-authors.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Saga Lab HP
2 Genotyping protocol <PCR>
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC01144, B6;129S7-Heyl<tm1Kkb>/YsaRbrc

RBRC No. RBRC01144
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129S7-Heyl<tm1Kkb>/YsaRbrc
Former Common name hairy and enhancer of split-related 3 Knock in (LacZ)
H-2 Haplotype No Data
Donor strain 129S7/SvEvBrd via AB1 ES cell line
Background strain C57BL/6NJcl
Appearance
1 Appearance black
Genotype a/a , B/B , C/C
Strain development B6;129S7-Heyl<tm1Kkb>. The Heyl gene is a member of the hairy and enhancer of split related family of bHLH transcription factors, play important roles in mediating the Notch signaling pathway. It is suggested that the Heyl gene has a role of the differentiation of retina. This strain is useful for elucidation of the Notch-signaling pathway mechanism.
Strain description Developed by Drs. H. Kokubo and R.L. Johnson at University of Texas M.D. Anderson Cancer Center in 1999. Combined loxP site and lacZ-Neo cassette was transferred into Heyl gene.
Colony maintenance Sibling mating.
Homozygous mutant mice are fertile and display no obvious defects.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 4
Common name neo; neomycin;
Symbol description No Data
2 Symbol Heyl
Symbol name hairy and enhancer of split-related 3
Chromosome 4
Common name No Data
Symbol description No Data
3 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 4
Common name No Data
Symbol description E. coli beta galactosidase
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 4
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 4
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Saga Lab HP
2 Genotyping protocol <PCR>
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01373, CAG-CAT-Dll4#2

RBRC No. RBRC01373
Type TransgeneCartagena
Species Mus musculus
Strain name CAG-CAT-Dll4#2
Former Common name No Data
H-2 Haplotype No Data
Background strain ICR
Appearance
1 Appearance albino
Genotype c/c
Strain development Developed by Dr. Hiroki Kokubo, National Institute of Genetics, Japan in 2004. This strain was maintained under ICR background.
Strain description ICR.Cg-Tg(CAG-cat,-Dll4)2Ysa. In these transgenic mice, The Dll4, Delta-like 4 gene is expressed after excision of the floxed-CAT gene by Cre-mediated recombination.
Colony maintenance Homozygote x Hemizygote or Crossing to ICR (Jcl:MCH)
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Dll4
Symbol name Delta4
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
3 Symbol cat
Symbol name E. coli Chloramphenicol acetyltransferase (CAT)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
A permission of the DEPOSITOR is required. Within a period of 2 years after deposition of the material, user must contact the DEPOSITOR and notify him a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then user must include DEPOSITOR and/or developer’s names as co-authors.
Additional information
1 Saga Lab HP
2 Genotyping protocol <PCR>
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01862, ICR.Cg-Mesp2<tm(GFP)>/YsaRbrc

RBRC No. RBRC01862
Type Targeted MutationCartagena
Species Mus musculus
Strain name ICR.Cg-Mesp2<tm(GFP)>/YsaRbrc
Former Common name Mesp2-GFP
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 7
Common name No Data
Symbol description No Data
3 Symbol Mesp2
Symbol name mesoderm posterior 2
Chromosome 7
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
6 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 7
Common name neo; neomycin;
Symbol description No Data
References Dev Biol. 2006 Aug 24;.

Cooperative Mesp activity is required for normal somitogenesis along the anterior-posterior axis.
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Dev. Biol., 300, 687-698 (2006).
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Saga Lab HP
2 Genotyping protocol <PCR>
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Scaling of embryonic patterning based on phase-gradient encoding. Nature. (2012).(23254931)
Lauschke VM, Tsiairis CD, Fran ois P, Aulehla A.

RBRC02490, ICR.Cg-Tg(P2APdeltaEME)2Ysa/YsaRbrc

RBRC No. RBRC02490
Type TransgeneCartagena wks
Species Mus musculus
Strain name ICR.Cg-Tg(P2APdeltaEME)2Ysa/YsaRbrc
Former Common name BAC-P2AP Delta EME#2
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol ALP
Symbol name alkaline phosphatase (human)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome UN
Common name neo; neomycin;
Symbol description No Data
References Mech Dev. 2008 May-Jun;125(5-6):432-40. Epub 2008 Feb 5.

Identification of presomitic mesoderm (PSM)-specific Mesp1 enhancer and generation of a PSM-specific Mesp1/Mesp2-null mouse using BAC-based rescue technology.
Research applications Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mech Dev., 125, 432-440 (2008).
Additional information
1 Saga Lab HP
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02561, ICR.Cg-Lfng<tm1(venus)Ysa>/YsaRbrc

RBRC No. RBRC02561
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name ICR.Cg-Lfng<tm1(venus)Ysa>/YsaRbrc
Former Common name L-fring-venus knockin(+neo)
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Venus
Symbol name Green Fluorescent Protein, F46L (Jellyfish)
Chromosome 5
Common name Venus
Symbol description No Data
2 Symbol Lfng
Symbol name LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
Chromosome 5
Common name lunatic fringe
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 5
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 5
Common name neo; neomycin;
Symbol description No Data
References No Data

Research applications Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
Within a period of two years after deposition of the BIOLOGICAL RESOURCE, the RECIPIENT shall obtain prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR and notify him/her a content of the research. If the DEPOSITOR judges that the research should be a collaborative work, then the RECIPIENT must include the DEPOSITOR and/or the DEVELOPER’s names as co-authors.
Additional information
1 Saga Lab HP
Depositor Saga, Yumiko (National Institute of Genetics) Saga, Yumiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months