RBRC05555, B6;B6129-Ezh2<tm1Yugo>/Hko

RBRC No. RBRC05555
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;B6129-Ezh2<tm1Yugo>/Hko
Former Common name B6-Ezh2 floxed
H-2 Haplotype No Data
ES cell line M1 [(C57BL/6 x 129)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Haruhiko Koseki and Jun Shinga, RIKEN Center for Allergy and Immunology in 2009. M1 ES cells derived from (C57BL/6 x 129) F1 were used. The mutant mice were crossed to C57BL/6J.
Strain description Ezh2 floxed mice. A floxed neo cassette was inserted upstream of exon 18 and an additional loxP was inserted downstream of exon 19 of Ezh2 gene. Conditional Ezh2 deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report
BRC facility Health Report in the last year and a half

Examination Date Room Rack
Sep 9, 2013 2-検 E
Jul 8, 2013 2-検 E
May 13, 2013 2-検 E
Mar 4, 2013 2-検 E
Jan 7, 2013 2-検 E

Gene Details
Promoter No Data
1 Symbol Ezh2
Symbol name enhancer of zeste homolog 2 (Drosophila)
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name neo; neomycin;
Symbol description No Data
References

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Koseki, Haruhiko (RIKEN Research Center For Allergy and Immunology) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06215, B6.Cg-Plag1<tm1.2Hko>

RBRC No. RBRC06215
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Plag1<tm1.2Hko>
Former Common name PLAG1 Delta FRT/+ (GWAS011), B6-Plag1 floxed
H-2 Haplotype No Data
ES cell line M1 [(C57BL/6 x 129)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Haruhiko Koseki, RIKEN Center for Allergy and Immunology. M1 ES cells derived from (C57BL/6 x 129)F1 were used for the generation of floxed mice. Neo cassette was removed by crossing with ACTB-FLPe mice (C57BL/6 background). The mutant mice were crossed to C57BL/6J.
Strain description PLAG1 (Plag1) gene floxed mice. Exon 5 was flanked by loxP sites.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 4
Common name No Data
Symbol description No Data
2 Symbol Plag1
Symbol name pleiomorphic adenoma gene 1
Chromosome 4
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 4
Common name No Data
Symbol description No Data
References Unpublished

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Koseki, Haruhiko (RIKEN Center for Integrative Medical Sciences (IMS)) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC09906, B6;B6D2-Fgf9<Eks>

RBRC No. RBRC09906
Type Spontaneous Mutation
Species Mus musculus
Strain name B6;B6D2-Fgf9<Eks>
Former Common name Fgf9Eks
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Fgf9
Symbol name fibroblast growth factor 9
Chromosome 14
Common name No Data
Symbol description No Data
References No Data

Research applications Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Koseki, Haruhiko (RIKEN Center for Integrative Medical Sciences (IMS)) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC10847, B6;B6129-Pcgf3<tm1.1Hko>

RBRC No. RBRC10847
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;B6129-Pcgf3<tm1.1Hko>
Former Common name Pcgf3
H-2 Haplotype No Data
ES cell line M1 [(C57BL/6 x 129)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 5
Common name No Data
Symbol description No Data
2 Symbol Pcgf3
Symbol name polycomb group ring finger 3
Chromosome 5
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 5
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
Depositor Koseki, Haruhiko (RIKEN Research Center For Allergy and Immunology) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC05572, B6;129-Rnf2<tm2Hko>

RBRC No. RBRC05572
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129-Rnf2<tm2Hko>
Former Common name B6-Rnf2 floxed Ring1B cond
H-2 Haplotype No Data
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Haruhiko Koseki, RIKEN Center for Allergy and Immunology, and Miguel Vidal, Centro de Investigaciones Biologicas in 2002. R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+> were used. The mutant mice were crossed to C57BL/6J at least 5 times.
Strain description Ring1B (Rnf2) gene floxed mice. A loxP site was inserted upstream of Exon 2 including the ATG codon. A floxed neo cassette was inserted into intron 2.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 1
Common name neo; neomycin;
Symbol description No Data
2 Symbol Rnf2
Symbol name ring finger protein 2
Chromosome 1
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 1
Common name No Data
Symbol description No Data
References Dev Cell. 2004 Nov;7(5):663-76.

Polycomb group proteins Ring1A/B link ubiquitylation of histone H2A to heritable gene silencing and X inactivation.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Koseki, Haruhiko (RIKEN Research Center For Allergy and Immunology) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06216, B6.Cg-Mc4r<tm1.2Hko>

RBRC No. RBRC06216
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Mc4r<tm1.2Hko>
Former Common name MCR Delta FRT/+ (GWAS023), B6-Mc4r floxed
H-2 Haplotype No Data
ES cell line M1 [(C57BL/6 x 129)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Haruhiko Koseki, RIKEN Center for Allergy and Immunology. M1 ES cells derived from (C57BL/6 x 129)F1 were used for the generation of floxed mice. Neo cassette was removed by crossing with ACTB-FLPe mice (C57BL/6 background). The mutant mice were crossed to C57BL/6J.
Strain description MCR4 (Mcr4) gene floxed mice. Exon 1 was flanked by loxP sites.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 18
Common name No Data
Symbol description No Data
2 Symbol Mc4r
Symbol name melanocortin 4 receptor
Chromosome 18
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 18
Common name No Data
Symbol description No Data
References Unpublished

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Koseki, Haruhiko (RIKEN Center for Integrative Medical Sciences (IMS)) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC10210, B6;Cg-Ewsr1<tm1.1Hko>

RBRC No. RBRC10210
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6;Cg-Ewsr1<tm1.1Hko>
Former Common name Ewsr1 cond.
H-2 Haplotype No Data
ES cell line M1 [(C57BL/6 x 129)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Ewsr1
Symbol name Ewing sarcoma breakpoint region 1
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 11
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Koseki, Haruhiko (RIKEN Center for Integrative Medical Sciences (IMS)) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC10848, B6;B6129-Pcgf5<tm1.1Hko>

RBRC No. RBRC10848
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;B6129-Pcgf5<tm1.1Hko>
Former Common name Pcgf5
H-2 Haplotype No Data
ES cell line M1 [(C57BL/6 x 129)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 19
Common name No Data
Symbol description No Data
2 Symbol Pcgf5
Symbol name polycomb group ring finger 5
Chromosome 19
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 19
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
Depositor Koseki, Haruhiko (RIKEN Research Center For Allergy and Immunology) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC05638, B6;129-Nov<tm1Hko>

RBRC No. RBRC05638
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129-Nov<tm1Hko>
Former Common name CCN3 KO, B6-Nov KO
H-2 Haplotype No Data
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Haruhiko Koseki, RIKEN Center for Allergy and Immunology. R1 ES cells derived from (129X1/SvJ x 129S1/Sv)F1-Kitl<+> were used for the generation of knockout mice. The mutant mice were crossed to C57BL/6J.
Strain description Nov (CCN3) gene knockout mice. Exons 1, 2 and 3 of Nov gene were replaced with a neomycin selection cassette. Homozygous mutant mice show no obvious abnormality. Homozygous mutant mice exhibit enhanced neointimal hyperplasia in vascular smooth muscles induced by endothelial injury.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Nov
Symbol name nephroblastoma overexpressed gene
Chromosome 15
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 15
Common name neo; neomycin;
Symbol description No Data
References

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Koseki, Haruhiko (RIKEN Research Center For Allergy and Immunology) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06536, B6.Cg-Uhrf1<tm2.1Hko>

RBRC No. RBRC06536
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Uhrf1<tm2.1Hko>
Former Common name NP95 cond ver.2, 6-Uhrf1 floxed
H-2 Haplotype No Data
ES cell line M1 [(C57BL/6 x 129)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Haruhiko Koseki, RIKEN Center for Integrative Medical Sciences. M1 ES cell were used to generate the mutant mice.
Strain description Uhrf1 gene floxed mice. Gene targeting for the Np95 (also known as Uhrf1, ICBP90) allele was done by flanking exons 4 and 5 with Cre-loxP sequences. Conditional deletion of Np95 (exons 4,5) can be achieved by crossing the mice with specific cre deleters such as CreERT2. The Np95 protein is an epigenetic reader and writer molecule, involved in maintenance DNA methylation (Sharif et al., 2007), recognition of H3K9 di/tri methylation (Rothbart et al., 2012) and ubiquitination of histone H3 (Nishiyama et al., 2013). The constitutive null (Np95-KO) mice are embryonically lethal post 9.5 dpc. Ablation of Np95 causes genomewide loss of DNA methylation marks (Sharif et al., 2007). This conditional allele would be useful to study the roles of Np95 in DNA methylation and regulation of other epigenetic modifications such as H3K9 methylation and H3 ubiquitination, by combining with tissue specific Cre deleters
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 17
Common name No Data
Symbol description No Data
2 Symbol Uhrf1
Symbol name ubiquitin-like, containing PHD and RING finger domains, 1
Chromosome 17
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 17
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Mouse of the Month May 2015
2 Genotyping protocol <PCR>
Depositor Koseki, Haruhiko (RIKEN Center for Integrative Medical Sciences (IMS)) Koseki, Haruhiko
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months