RBRC00158, C57BL/6-TgN(gpt Delta)

RBRC No. RBRC00158
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-TgN(gpt Delta)
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6
Appearance
1 Appearance black
Genotype a/a
Strain development This strain was developed for efficient detection of mutations in vivo by Dr.
Nohmi T.
Strain description This strain is useful to detect point mutations and deletions with high efficiency and sensitivity in vivo. The mice carry lambda EG10 DNA as a transgene. When the rescued phages are infected into Escherichia coli YG6020-expressing Cre recombinase, the phage DNA is converted into plasmid pYG142 carrying the chloramphenicol-resistance gene and the gpt gene of E. coli. The gpt mutants can be positively detected as colonies arising on plates containing chloramphenicol and 6-thioguanine. The EG10 DNA carries a chi site along with the red and gam genes so that the wild-type phages display Spi-(sensitive to P2 interference) phenotype. Mutant phages lacking both red and gam genes can be positively detected as plaques that grow in P2 lysogens of E. coli.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol cat
Symbol name E. coli Chloramphenicol acetyltransferase (CAT)
Chromosome 17
Common name No Data
Symbol description No Data
2 Symbol gpt Delta
Symbol name guanine phosphoribosyltransferase
Chromosome 17
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 17
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 17
Common name No Data
Symbol description No Data
5 Symbol red/gam
Symbol name lambda phage red/gam
Chromosome 17
Common name No Data
Symbol description No Data
References Environ. Mutagen Res., 22, 85-90 (2000).
Cancer Lett. 1999 Sep 1;143(2):241-4.
Cancer Res. 2001 May 15;61(10):3913-8.
Carcinogenesis. 2000 Nov;21(11):2049-56.
Environ Mol Mutagen. 1996;28(4):465-70.
Environ Mol Mutagen. 1999;34(1):1-8.
Environ Mol Mutagen. 1999;34(1):9-15.
Environ Mol Mutagen. 1999;34(2-3):72-9.
Proc Natl Acad Sci U S A. 2000 Apr 11;97(8):4156-61.

Research applications No Data
Specific Term and Conditions No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
Additional information
1 No Data
Depositor Noumi, Takehiko (National Institute of Health Sciences) Noumi, Takehiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC06054, C57BL/6-Polk<tm3(mpolk)Csk> Tg(gptdelta)1Nmi

RBRC No. RBRC06054
Type Targeted Mutation and TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Polk<tm3(mpolk)Csk> Tg(gptdelta)1Nmi
Former Common name inactivated Polk KI and gpt delta double transgenic mice
H-2 Haplotype No Data
ES cell line Not specified (ES cell)
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takehiko Nohmi, National Institute of Health Sciences. Inactivate Polk KI mice were generated by using C57BL/6N derived ES cells. The mutant mice were crossed with the gpt delta Tg mice (C57BL/6J background) (RBRC00158). C57BL/6J and C57BL/6N mixed background.
Strain description Inactivate Polk knock-in mice, in which Asp at position 197 and Glu at position 198 of exon 6 were replace with Ala, respectively. This mutant mice also have a gpt delta transgene that serves as a tool for assessing the mutagenicity of environmental chemicals.
Colony maintenance KI: Heterozygote x Wild-type; Tg: Carrier x Carrier
Health Report
Gene Details
Promoter No Data
1 Symbol Polk
Symbol name polymerase (DNA directed), kappa
Chromosome 13
Common name No Data
Symbol description No Data
2 Symbol Polk
Symbol name polymerase (DNA directed), kappa
Chromosome 13
Common name No Data
Symbol description No Data
3 Symbol cat
Symbol name E. coli Chloramphenicol acetyltransferase (CAT)
Chromosome 17
Common name No Data
Symbol description No Data
4 Symbol gpt Delta
Symbol name guanine phosphoribosyltransferase
Chromosome 17
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 17
Common name No Data
Symbol description No Data
6 Symbol red/gam
Symbol name lambda phage red/gam
Chromosome 17
Common name No Data
Symbol description No Data
References Unpublished

Research applications Cancer Research,
Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must reach agreement on terms and conditions of use of the mice with the DEPOSITOR and its co-developer, Chugai Pharmaceutical Co., Ltd. (Contact person: Dr. Akira Takeiri, e-mail, takeiriakr@chugai-pharm.co.jp) and must obtain a prior written consent from them and, if necessary, shall enter into an agreement with them.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Noumi, Takehiko (National Institute of Health Sciences) Noumi, Takehiko
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :