RBRC01251, C57BL/6-Tg(EIF1A-FLP)14Mim/MimRbrc

RBRC No. RBRC01251
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(EIF1A-FLP)14Mim/MimRbrc
Former Common name FLP14; FLP14 mouse
H-2 Haplotype No Data
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol poly A
Symbol name human granulocyte colony-stimulating facter gene poly adenylation signal sequence
Chromosome UN
Common name No Data
Symbol description No Data
Promoter human elongation factor 1 alpha (EF-1 alpha) promoter
3 Symbol FLP
Symbol name yeast flippase recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References Biochem Biophys Res Commun. 2002 May 10;293(3):953-7.

Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting.
Research applications Cell Biology Research,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Biochem. Biophys. Res. Commun., 293, 953-957 (2002).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Mishina, Masayoshi (The University of Tokyo, Graduate School of Medicine) Mishina, Masayoshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01252, C57BL/6-Tg(EEF1A1-FLP)66Mim/MmshRbrc

RBRC No. RBRC01252
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(EEF1A1-FLP)66Mim/MmshRbrc
Former Common name FLP66, FLP66 mouse
H-2 Haplotype No Data
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Tomonori Takeuchi and Masayoshi Mishina, Graduate School of Medicine, University of Tokyo in 1997. FLP recombinase rexpression vector pEF-NFLP was injected into the pronuclei of C57BL/6NCrj fertilized eggs. Three transgenic mouse lines (FLP22, FLP39, and FLP66) were produced.
Strain description These transgenic mice express a FLP recombinase gene under the control of the human polypeptide chian elongation factor 1 alpha (EF-1alpha) gene promoter. This can be used to make an inducible knock-out by corssing with the other genetically-engineeered mice that have a pair of FLP recombinase target (FRT) sequence flanking the genomic region of interest. This strain is useful to remove the drug-resistance marker gene from mice manipulated for the conditional gene targeting using the Cre/loxP system.
Colony maintenance Carrier x Carrier [or Crossing to C57BL/6NCrlCrlj]. Tg homozygous and hemizygous mice are viable and fertile.
Health Report
Gene Details
Promoter No Data
1 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol poly A
Symbol name human granulocyte colony-stimulating factor gene poly adenylation signal sequence
Chromosome UN
Common name No Data
Symbol description No Data
Promoter human elongation factor 1 alpha (EF-1 alpha) promoter
3 Symbol FLP
Symbol name yeast flippase recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References Biochem Biophys Res Commun. 2002 May 10;293(3):953-7.

Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting.
Research applications Cell Biology Research,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Biochem. Biophys. Res. Commun., 293, 953-957 (2002).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Mishina, Masayoshi (The University of Tokyo, Graduate School of Medicine) Mishina, Masayoshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Phospholipase Cgamma2 is necessary for separation of blood and lymphatic vasculature in mice. Development.136(2): 191-5 (2009).(19056831)
Ichise H, Ichise T, Ohtani O, Yoshida N.
H-, N- and Kras cooperatively regulate lymphatic vessel growth by modulating VEGFR3 expression in lymphatic endothelial cells in mice. Development.137(6): 1003-13 (2010).(20179099)
Ichise T, Yoshida N, Ichise H.

RBRC01655, GluRδ2-tTA mouse

RBRC No. RBRC01655
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name GluRδ2-tTA mouse
Former Common name GluRδ2-tTA mouse
H-2 Haplotype No Data
Donor strain C57BL/6
Background strain C57BL/6NJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Grid2
Symbol name glutamate receptor, ionotropic, delta 2
Chromosome 6
Common name B230104L07Rik, GluRdelta2, tpr
Symbol description No Data
2 Symbol tTA
Symbol name tTA (E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain)
Chromosome 6
Common name E. coli tet repressor (TetR), herpes simplex virus (HSV)VP16 activation domain
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
References No Data

Research applications FLP/frt system,
Tet system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Prior to filing for a patent, or intellectual property or other rights based on results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research. And Use of the BIOLOGICAL RESOURCE shall require co-authorship of the DEPOSITOR for TWO years after deposition of the BIOLOGICAL RESOURCE to the RIKEN BRC or for first publication if no publication is made within the TWO years.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Mishina, Masayoshi (The University of Tokyo, Graduate School of Medicine) Mishina, Masayoshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01656, C57BL/6-Kcnh5<tm1(cre/PGR)Mim>/MimRbrc

RBRC No. RBRC01656
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name C57BL/6-Kcnh5<tm1(cre/PGR)Mim>/MimRbrc
Former Common name Eag2-hCrePR mouse
H-2 Haplotype No Data
Donor strain C57BL/6
Background strain C57BL/6NJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol PGR
Symbol name Progesterone receptor (human)
Chromosome 12
Common name 9930019P03Rik, NR3C3, PR, PR-A, PR-B
Symbol description No Data
2 Symbol Kcnh5
Symbol name potassium voltage-gated channel, subfamily H (eag-related), member 5
Chromosome 12
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 12
Common name No Data
Symbol description No Data
Promoter Eag2
4 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome 12
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 12
Common name neo; neomycin;
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Prior to filing for a patent, or intellectual property or other rights based on results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research. And Use of the BIOLOGICAL RESOURCE shall require co-authorship of the DEPOSITOR for TWO years after deposition of the BIOLOGICAL RESOURCE to the RIKEN BRC or for first publication if no publication is made within the TWO years.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Mishina, Masayoshi (The University of Tokyo, Graduate School of Medicine) Mishina, Masayoshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01657, GluRδ2KO mouse

RBRC No. RBRC01657
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name GluRδ2KO mouse
Former Common name GluRδ2KO mouse
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Donor strain (C57BL/6 x CBA)F1 via TT2 ES cell line
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Grid2
Symbol name glutamate receptor, ionotropic, delta 2
Chromosome 6
Common name B230104L07Rik, GluRdelta2, tpr
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name neo; neomycin;
Symbol description No Data
References Cell. 1995 Apr 21;81(2):245-52.

Impairment of motor coordination, Purkinje cell synapse formation, and cerebellar long-term depression in GluR delta 2 mutant mice.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Prior to filing for a patent, or intellectual property or other rights based on results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research. And Use of the BIOLOGICAL RESOURCE shall require co-authorship of the DEPOSITOR for TWO years after deposition of the BIOLOGICAL RESOURCE to the RIKEN BRC or for first publication if no publication is made within the TWO years.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Mishina, Masayoshi (The University of Tokyo, Graduate School of Medicine) Mishina, Masayoshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01813, B6.Cg-Grin2a<tm1Mim>/MimRbrc

RBRC No. RBRC01813
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.Cg-Grin2a<tm1Mim>/MimRbrc
Former Common name GluRε1 KO mouse
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Masayoshi Mishina at Tokyo University in 1995. The targeting vector containing pgk-neo cassette was transferred into TT2 ES cells to replace the exon encoding M2 and M3 transmembrane regions of Grin2a gene. The Grin2a deficient mice were backcrossed to C57BL/6N for 14 times.
Strain description B6.Cg-Grin2a<tm1Mim>. The Grin2a gene is one of the glutamate receptor channel subunit families forming NMDA receptor channel, which is important for synaptic plasticity. The Grin2a homozygous mutant mice show a moderate deficiency in spatial learning. Reproductive performance and growth of them is normal.
Colony maintenance backcross to C57BL/6 (Heterozygote x C57BL/6NJcl)
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Grin2a
Symbol name glutamate receptor, ionotropic, NMDA2A (epsilon 1)
Chromosome 16
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 16
Common name neo; neomycin;
Symbol description No Data
References J Neurosci. 1998 Sep 1;18(17):6704-12.

Increased thresholds for long-term potentiation and contextual learning in mice lacking the NMDA-type glutamate receptor epsilon1 subunit.
Increased thresholds for long-term potentiation and contextual learning in mice lacking the NMDA-type glutamate receptor epsilon1 subunit.
Research applications Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature, 373, 151-155 (1995).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Prior to filing for a patent, or intellectual property or other rights based on results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research. And Use of the BIOLOGICAL RESOURCE shall require co-authorship of the DEPOSITOR for TWO years after deposition of the BIOLOGICAL RESOURCE to the RIKEN BRC or for the first publication if no publication is made within the TWO years.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Mishina, Masayoshi (The University of Tokyo, Graduate School of Medicine) Mishina, Masayoshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Phenotypic characterization of orofacial movement topography in mutants with disruption of amino acid mechanisms: Glutamate N2A/B/D [GluR 1/2/4] subtypes and the GABA synthesizing enzyme GAD65. Neuroscience.250: 743-54 (2013).(23892010)
Tomiyama K, Kato R, Hara Y, Kobayashi M, Mishina M, Yanagawa Y, Kinsella A, Koshikawa N, Waddington JL.

RBRC01814, B6.Cg-Grin2b<tm1Mim>/MimRbrc

RBRC No. RBRC01814
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.Cg-Grin2b<tm1Mim>/MimRbrc
Former Common name GluRε2 KO mouse
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Masayoshi Mishina at Tokyo University in 1995. A pgk-neo was transfered into TT2 ES cells to replace the exon encoding the transiation initiation site of Grin2b gene. The Mint deficient mice were backcrossed to C57BL/6N for 14 times.
Strain description B6.Cg-Grin2b<tm1Mim>. The Grin2b gene is one of the glutamate recepoter channel subunit families forming NMDA receptor channel, which is important for synaptic plasticity. Homozygous mutant mice show impairments in suckling and die shortly after birth.
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6NJcl)
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Grin2b
Symbol name glutamate receptor, ionotropic, NMDA2B (epsilon 2)
Chromosome 6
Common name GluRepsilon2, NMDAR2B, Nmdar2b, NR2B
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name neo; neomycin;
Symbol description No Data
References Eur J Neurosci. 2001 Jul;14(1):153-60.
Neuron. 1996 Feb;16(2):333-44.

Roles of the glutamate receptor epsilon2 and delta2 subunits in the potentiation and prepulse inhibition of the acoustic startle reflex.
Roles of the glutamate receptor epsilon2 and delta2 subunits in the potentiation and prepulse inhibition of the acoustic startle reflex.
Research applications Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neuron, 16 ,333-344 (1996).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Prior to filing for a patent, or intellectual property or other rights based on results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research. And Use of the BIOLOGICAL RESOURCE shall require co-authorship of the DEPOSITOR for TWO years after deposition of the BIOLOGICAL RESOURCE to the RIKEN BRC or for the first publication if no publication is made within the TWO years.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Mishina, Masayoshi (The University of Tokyo, Graduate School of Medicine) Mishina, Masayoshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Phenotypic characterization of orofacial movement topography in mutants with disruption of amino acid mechanisms: Glutamate N2A/B/D [GluR 1/2/4] subtypes and the GABA synthesizing enzyme GAD65. Neuroscience.250: 743-54 (2013).(23892010)
Tomiyama K, Kato R, Hara Y, Kobayashi M, Mishina M, Yanagawa Y, Kinsella A, Koshikawa N, Waddington JL.

RBRC01840, B6.Cg-Grin2d<tm1Mim>/MimRbrc

RBRC No. RBRC01840
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Grin2d<tm1Mim>/MimRbrc
Former Common name GluRε4 KO mouse
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain C57BL/6
Appearance
1 Appearance black
Genotype No Data
Strain development Developed by Kazutaka Ikeda and Masami Mishina, Brain Research Institute, Niigata University in 1995. A pgk-neo was transfered into TT2 ES cells to replace the exon encoding the M4 putative transmembrane segment of Grin2d gene. The mutant mice were backcrossed to C57BL/6N over 10 times.
Strain description GluR epsilon4 (Grin2d, glutamate receptor, ionotropic, NMDA2D (epsilon 4)) knockout mice. The Grin2d gene is one of the glutamate receptor channel subunit families forming NMDA receptor channel. The Grin2d homozygous mutant mice show a reduced spontaneous activity, but no significant difference in motor activity and anxiety tests when compared with normal mice. Reproductive performance and growth of them is normal.
Colony maintenance Heterozygote x Wild-type [C57BL/6NJcl]. Homozygous mutant mice are viable and fertile.
Health Report
Gene Details
Promoter No Data
1 Symbol Grin2d
Symbol name glutamate receptor, ionotropic, NMDA2D (epsilon 4)
Chromosome 7
Common name GluRepsilon4, NMDAR2D, NR2D
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 7
Common name neo; neomycin;
Symbol description No Data
References

Research applications Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol. Brain. Res., 33, 61-71 (1995).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Prior to filing for a patent, or intellectual property or other rights based on results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research. And Use of the BIOLOGICAL RESOURCE shall require co-authorship of the DEPOSITOR for TWO years after deposition of the BIOLOGICAL RESOURCE to the RIKEN BRC or for the first publication if no publication is made within the TWO years.
Additional information
1 Genetic Background
2 Lab HP
3 Genotyping protocol <PCR>
Depositor Mishina, Masayoshi (The University of Tokyo, Graduate School of Medicine) Mishina, Masayoshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
GluN2D Subunit-Containing NMDA Receptors Control Tissue Plasminogen Activator-Mediated Spatial Memory. J Neurosci.32(37): 12726-34 (2012).(22972996)
Obiang P, Macrez R, Jullienne A, Bertrand T, Lesept F, Ali C, Maubert E, Vivien D, Agin V.
Phenotypic characterization of orofacial movement topography in mutants with disruption of amino acid mechanisms: Glutamate N2A/B/D [GluR 1/2/4] subtypes and the GABA synthesizing enzyme GAD65. Neuroscience.250: 743-54 (2013).(23892010)
Tomiyama K, Kato R, Hara Y, Kobayashi M, Mishina M, Yanagawa Y, Kinsella A, Koshikawa N, Waddington JL.

RBRC02694, B6.Cg-Ptk2<tm1Mmsh>

RBRC No. RBRC02694
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Ptk2<tm1Mmsh>
Former Common name Ptk2, fFak
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Ptk2
Symbol name PTK2 protein tyrosine kinase 2
Chromosome 15
Common name Fadk, FAK, FRNK
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 15
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 15
Common name No Data
Symbol description No Data
References Biochem Biophys Res Commun. 2002 May 10;293(3):953-7.
Eur J Neurosci. 2008 Feb;27(4):836-54. Epub 2008 Feb 13.

Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting.
Flp recombinase transgenic mice of C57BL/6 strain for conditional gene targeting.
Research applications Cell Biology Research,
Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Eur. J. Meurosci., 27, 836-854 (2008).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Prior to filing for a patent, or intellectual property or other rights based on results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research. Use of the BIOLOGICAL RESOURCE shall require co-authorship of the DEPOSITOR for TWO years after deposition of the BIOLOGICAL RESOURCE to the RIKEN BRC or for the first publication if no publication is made within the TWO years.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Mishina, Masayoshi (The University of Tokyo, Graduate School of Medicine) Mishina, Masayoshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02695, B6.Cg-Grid2ip<tm1.1Mmsh>

RBRC No. RBRC02695
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Grid2ip<tm1.1Mmsh>
Former Common name Grid2ip KO, Delphilin KO
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Grid2ip
Symbol name glutamate receptor, ionotropic, delta 2 (Grid2) interacting protein 1
Chromosome 5
Common name delphilin
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 5
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 5
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 5
Common name neo; neomycin;
Symbol description No Data
References PLoS ONE. 2008 May 28;3(5):e2297.

Enhancement of both long-term depression induction and optokinetic response adaptation in mice lacking delphilin.
Research applications Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
PLoS ONE, 3, e2297 (2008).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Prior to filing for a patent, or intellectual property or other rights based on results of research using the BIOLOGICAL RESOURCE, the RECIPIENT shall acquire the consent from the DEPOSITOR for such application. Use of the BIOLOGICAL RESOURCE shall be limited to a collaborative research. Use of the BIOLOGICAL RESOURCE shall require co-authorship of the DEPOSITOR for TWO years after deposition of the BIOLOGICAL RESOURCE to the RIKEN BRC or for the first publication if no publication is made within the TWO years.
Additional information
1 Lab HP
2 Mouse of the Month Aug 2008
3 Genotyping protocol <PCR>
Depositor Mishina, Masayoshi (The University of Tokyo, Graduate School of Medicine) Mishina, Masayoshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months