RBRC02712, B6.Cg-Gpr126<tm1Taki>

RBRC No. RBRC02712
Type Targeted Mutation CongenicCartagena wks
Species Mus musculus
Strain name B6.Cg-Gpr126<tm1Taki>
Former Common name DREG Knock-out Mouse
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain C57BL/6
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Akiyama T, The University of Tokyo.
Strain description Gpr129 knock-out mice. Gpr129, G-protein-coupled receptor 126 is a member of seven-transmembrane proteins related to family-B G-protein- coupled receptors (LNB-TM7), expresses at high levels in the heart and somites during mouse embryogenesis and in the adult lung. Homozygous mutant mice are embryonic lethal (E12.5).
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Gpr126
Symbol name G protein-coupled receptor 126
Chromosome 10
Common name 1190004A11Rik, DREG, LOC215798
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 10
Common name No Data
Symbol description E. coli beta galactosidase
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 10
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 10
Common name neo; neomycin;
Symbol description No Data
References Genes Cells 2004 Jun;9(6):549-60.

DREG, a developmentally regulated G protein-coupled receptor containing two conserved proteolytic cleavage sites.
Research applications Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genes Cells, 9, 549-560 (2004).
The DEPOSITOR should be a co-author in articles when the RECIPIENTs publish their data using the BIOLOGICAL RESOURCE for two years after deposition to the RIKEN BRC. After two years from deposition, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Akiyama, Tetsu (The University of Tokyo) Akiyama, Tetsu
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02149, B6.Cg-Ctnnbip1<tm1Taki>

RBRC No. RBRC02149
Type Targeted Mutation CongenicCartagena wks
Species Mus musculus
Strain name B6.Cg-Ctnnbip1<tm1Taki>
Former Common name ICAT Knock-out mouse
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Akiyama at Institute for Molecular and Cellular Bioscviences, University of Tokyo in 2001.
Strain description ICAT, Inhibitor of beta-catenin and Tcell factor is an 81 amino acid protein that interferes with the interaction between beta-catenin and TCF. The ICAT gene ubiquitously expressed in embryo, and brain, heart, liver and skeletal muscle in adult of mouse. ICAT homozygous mutant mice show malformation of the forebrain and craniofacial bones and lack kidney, and dead within 1 day after birth.
Colony maintenance Heterozygote x C57BL/6 [C57BL/6NJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Ctnnbip1
Symbol name catenin beta interacting protein 1
Chromosome 4
Common name 1110008O09Rik, 2310001I19Rik, Catnbip1, ICAT
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 4
Common name neo; neomycin;
Symbol description No Data
References Proc Natl Acad Sci U S A 2004 May 25;101(21):8017-21. Epub 2004 May 17.

Anteriorization of neural fate by inhibitor of beta-catenin and T cell factor (ICAT), a negative regulator of Wnt signaling.
Research applications Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Proc. Natl. Acad. Sci. USA, 101, 8017-8021 (2004).
The RECIPIENT of BIOLOGICAL RESOURCE shall inform the DEPOSITOR about the research project using the BIOLOGICAL RESOURCE, and agrees to include the DEPOSITOR as a co-author in any publications resulted by the used of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC. After the two years’ period the RECIPIENT shall acknowledge to the DEPOSITOR in any publications resulted by the use of the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP (Japanese)
2 Genetic Background
3 Genotyping protocol <PCR>
Depositor Akiyama, Tetsu (The University of Tokyo) Akiyama, Tetsu
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months