RBRC03436, B6;129P2-Arx<tm1Kki>/2Rbrc

RBRC No. RBRC03436
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Arx<tm1Kki>/2Rbrc
Former Common name Arx-2 KO
H-2 Haplotype No Data
ES cell line E14TG2a [129P2/OlaHsd]carries Hprt
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Kunio Kitamura, Mitsubishi Kagaku Institute of Life Sciences. E14 ES cells were used to generate the mutant mice.
Strain description Arx gene knockout mice. A lacZ-neo cassette was inserted into the exon 2 of Arx gene. Hemizygous (-/Y) mutant males die within 12 hrs after birth. Main line: Arx-6 KO (RBRC03438).
Colony maintenance Heterozygote (female) x Wild-type (male)[C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Arx
Symbol name aristaless related homeobox
Chromosome X
Common name No Data
Symbol description No Data
References No Data

Research applications Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The BIOLOGICAL RESOURCE shall be used only for academic research purposes by
a non-profit organization.
The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and
negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL
RESOURCE for purposes of other than academic research.
In publishing the research results to be obtained by use of the BIOLOGICAL
RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the
source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi
Chemical Corporation.
The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical
Corporation of RECIPIENT organization, RECIPIENT name, the specific research
purpose and the date of distribution.
Additional information
1 No Data
Depositor Kitamura, Kunio (Mitsubishi Chemical Corporation) Kitamura, Kunio
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC03665, ICR;129(B6)-Arx<tm(GCG)Kki>/7-2Rbrc

RBRC No. RBRC03665
Type Targeted MutationCartagena
Species Mus musculus
Strain name ICR;129(B6)-Arx<tm(GCG)Kki>/7-2Rbrc
Former Common name Arx (GCG)7-2 KI (ICR)
H-2 Haplotype No Data
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Kunio Kitamura, Mitsubishi Kagaku Institute of Life Sciences. CCE/EK.CCE ES cells were used to generate the mutant mice.
Strain description Arx GCG 7 repeats knock-in mice. 7 repeats of the GCG triple were inserted at the codon 330 of Arx gene. C57BL/6 mixed background (RBRC03654 and RBRC03656), 129 background (RBRC03655), C3H mixed background (RBRC03657), ICR mixed background (RBRC03665).
Colony maintenance Heterozygote x Wild-type [or Crlj:CD1(ICR)]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Arx
Symbol name aristaless related homeobox
Chromosome X
Common name No Data
Symbol description No Data
References No Data

Research applications Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The BIOLOGICAL RESOURCE shall be used only for academic research purposes by
a non-profit organization.
The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and
negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL
RESOURCE for purposes of other than academic research.
In publishing the research results to be obtained by use of the BIOLOGICAL
RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the
source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi
Chemical Corporation.
The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical
Corporation of RECIPIENT organization, RECIPIENT name, the specific research
purpose and the date of distribution.
Additional information
1 No Data
Depositor Kitamura, Kunio (Mitsubishi Chemical Corporation) Kitamura, Kunio
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC03437, B6;129P2-Arx<tm1Kki>/4Rbrc

RBRC No. RBRC03437
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Arx<tm1Kki>/4Rbrc
Former Common name Arx-4 KO
H-2 Haplotype No Data
ES cell line E14TG2a [129P2/OlaHsd]carries Hprt
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Kunio Kitamura, Mitsubishi Kagaku Institute of Life Sciences. E14 ES cells were used to generate the mutant mice.
Strain description Arx gene knockout mice. A lacZ-neo cassette was inserted into the exon 2 of Arx gene. Hemizygous (-/Y) mutant males die within 12 hrs after birth. Main line: Arx-6 KO (RBRC03438).
Colony maintenance Heterozygote (female) x Wild-type (male)[C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Arx
Symbol name aristaless related homeobox
Chromosome X
Common name No Data
Symbol description No Data
References No Data

Research applications Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The BIOLOGICAL RESOURCE shall be used only for academic research purposes by
a non-profit organization.
The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and
negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL
RESOURCE for purposes of other than academic research.
In publishing the research results to be obtained by use of the BIOLOGICAL
RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the
source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi
Chemical Corporation.
The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical
Corporation of RECIPIENT organization, RECIPIENT name, the specific research
purpose and the date of distribution.
Additional information
1 No Data
Depositor Kitamura, Kunio (Mitsubishi Chemical Corporation) Kitamura, Kunio
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC03666, STOCK Arx<tm1Kki> Gad1<tm1.1Tama>/Rbrc

RBRC No. RBRC03666
Type Targeted MutationCartagena
Species Mus musculus
Strain name STOCK Arx<tm1Kki> Gad1<tm1.1Tama>/Rbrc
Former Common name Arx-6 KO/GAD67 GFPdNeo KI
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Kunio Kitamura, Mitsubishi Kagaku Institute of Life Sciences.
Strain description Arx gene knockout and GAD67-GFP (Gad1-GFPdNeo) double mutant mice. Arx: A lacZ-neo cassette was inserted into the exon 2 of Arx gene. Hemizygous (-/Y) mutant males die within 12 hrs after birth. GAD67: An EGFP was inserted at the ATG codon in exon 1 of Gad1. Homozygous mutant mice are rethal. A loxP flanked neo was removed by crossing with cre mice.
Colony maintenance Arx: Heterozygote (female) x Wild-type (male); Gad1: Heterozygote x Wild-type [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Gad1
Symbol name glutamic acid decarboxylase 1
Chromosome 2
Common name No Data
Symbol description No Data
2 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome 2
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 2
Common name No Data
Symbol description No Data
4 Symbol Arx
Symbol name aristaless related homeobox
Chromosome X
Common name No Data
Symbol description No Data
5 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome X
Common name No Data
Symbol description No Data
6 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome X
Common name No Data
Symbol description No Data
7 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
8 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome X
Common name No Data
Symbol description No Data
References Nat Genet 2002 Nov;32(3):359-69. Epub 2002 Oct 15.

Mutation of ARX causes abnormal development of forebrain and testes in mice and X-linked lissencephaly with abnormal genitalia in humans.
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The BIOLOGICAL RESOURCE shall be used only for academic research purposes by
a non-profit organization.
The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and
negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL
RESOURCE for purposes of other than academic research.
In publishing the research results to be obtained by use of the BIOLOGICAL
RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the
source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi
Chemical Corporation.
The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical
Corporation of RECIPIENT organization, RECIPIENT name, the specific research
purpose and the date of distribution.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor Kitamura, Kunio (Mitsubishi Chemical Corporation) Kitamura, Kunio
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC03438, B6;129P2-Arx<tm1Kki>/6Rbrc

RBRC No. RBRC03438
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Arx<tm1Kki>/6Rbrc
Former Common name Arx-6 KO
H-2 Haplotype No Data
ES cell line E14TG2a [129P2/OlaHsd]carries Hprt
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Kunio Kitamura, Mitsubishi Kagaku Institute of Life Sciences. E14 ES cells were used to generate the mutant mice.
Strain description Arx gene knockout mice. A lacZ-neo cassette was inserted into the exon 2 of Arx gene. Hemizygous (-/Y) mutant males die within 12 hrs after birth. Main line: Arx-6 KO (RBRC03438).
Colony maintenance Heterozygote (female) x Wild-type (male)[C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome X
Common name No Data
Symbol description No Data
2 Symbol Arx
Symbol name aristaless related homeobox
Chromosome X
Common name No Data
Symbol description No Data
3 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome X
Common name No Data
Symbol description No Data
4 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome X
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome X
Common name No Data
Symbol description No Data
References Nat Genet 2002 Nov;32(3):359-69. Epub 2002 Oct 15.

Mutation of ARX causes abnormal development of forebrain and testes in mice and X-linked lissencephaly with abnormal genitalia in humans.
Research applications Fluorescent Proteins/lacZ System,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The BIOLOGICAL RESOURCE shall be used only for academic research purposes by
a non-profit organization.
The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and
negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL
RESOURCE for purposes of other than academic research.
In publishing the research results to be obtained by use of the BIOLOGICAL
RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the
source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi
Chemical Corporation.
The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical
Corporation of RECIPIENT organization, RECIPIENT name, the specific research
purpose and the date of distribution.
Additional information
1 No Data
Depositor Kitamura, Kunio (Mitsubishi Chemical Corporation) Kitamura, Kunio
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC03444, B6;129P2-Pitx2<tm1Kki>/Rbrc

RBRC No. RBRC03444
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Pitx2<tm1Kki>/Rbrc
Former Common name Pitx2 KO
H-2 Haplotype No Data
ES cell line E14TG2a [129P2/OlaHsd]carries Hprt
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Pitx2
Symbol name paired-like homeodomain transcription factor 2
Chromosome 3
Common name No Data
Symbol description No Data
2 Symbol Ires
Symbol name internal ribosomal entry site (EMCV)
Chromosome 3
Common name No Data
Symbol description No Data
3 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 3
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 3
Common name No Data
Symbol description No Data
References

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The BIOLOGICAL RESOURCE shall be used only for academic research purposes by
a non-profit organization.
The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and
negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL
RESOURCE for purposes of other than academic research.
In publishing the research results to be obtained by use of the BIOLOGICAL
RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the
source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi
Chemical Corporation.
The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical
Corporation of RECIPIENT organization, RECIPIENT name, the specific research
purpose and the date of distribution.
Additional information
1 No Data
Depositor Kitamura, Kunio (Mitsubishi Chemical Corporation) Kitamura, Kunio
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC03653, B6;129-Arx<tm(Dp)Kki>/Rbrc

RBRC No. RBRC03653
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129-Arx<tm(Dp)Kki>/Rbrc
Former Common name Arx Dp-7 KI (B6), Arx (GCG)dup KI (B6)
H-2 Haplotype No Data
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Arx
Symbol name aristaless related homeobox
Chromosome X
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The BIOLOGICAL RESOURCE shall be used only for academic research purposes by
a non-profit organization.
The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and
negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL
RESOURCE for purposes of other than academic research.
In publishing the research results to be obtained by use of the BIOLOGICAL
RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the
source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi
Chemical Corporation.
The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical
Corporation of RECIPIENT organization, RECIPIENT name, the specific research
purpose and the date of distribution.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Kitamura, Kunio (Mitsubishi Chemical Corporation) Kitamura, Kunio
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Reduced polyalanine-expanded Arx mutant protein in developing mouse subpallium alters Lmo1 transcriptional regulation. Hum Mol Genet. (2013).(24122442)
Lee K, Mattiske T, Kitamura K, Gecz J, Shoubridge C.

RBRC03654, B6;129-Arx<tm(GCG)Kki>/7-1Rbrc

RBRC No. RBRC03654
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129-Arx<tm(GCG)Kki>/7-1Rbrc
Former Common name Arx (GCG)7-1 KI (B6)
H-2 Haplotype No Data
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Kunio Kitamura, Mitsubishi Kagaku Institute of Life Sciences. CCE/EK.CCE ES cells were used to generate the mutant mice.
Strain description Arx GCG 7 repeats knock-in mice. 7 repeats of the GCG triple were inserted at the codon 330 of Arx gene. C57BL/6 mixed background (RBRC03654 and RBRC03656), 129 background (RBRC03655), C3H mixed background (RBRC03657), ICR mixed background (RBRC03665).
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 polyA signal
Chromosome X
Common name No Data
Symbol description No Data
2 Symbol Arx
Symbol name aristaless related homeobox
Chromosome X
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome X
Common name No Data
Symbol description No Data
References

Research applications Mouse Models for Human Disease,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The BIOLOGICAL RESOURCE shall be used only for academic research purposes by
a non-profit organization.
The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and
negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL
RESOURCE for purposes of other than academic research.
In publishing the research results to be obtained by use of the BIOLOGICAL
RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the
source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi
Chemical Corporation.
The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical
Corporation of RECIPIENT organization, RECIPIENT name, the specific research
purpose and the date of distribution.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Kitamura, Kunio (Mitsubishi Chemical Corporation) Kitamura, Kunio
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
An Epilepsy-Related ARX Polyalanine Expansion Modifies Glutamatergic Neurons Excitability and Morphology Without Affecting GABAergic Neurons Development. Cereb Cortex. (2012).(22628459)
Beguin S, Cr pel V, Aniksztejn L, Becq H, Pelosi B, Pallesi-Pocachard E, Bouamrane L, Pasqualetti M, Kitamura K, Cardoso C, Represa A.
Aristaless-Related Homeobox Plays a Key Role in Hyperplasia of the Pancreas Islet alpha-Like Cells in Mice Deficient in Proglucagon-Derived Peptides. PLoS One.8(5): e64415 (2013).(23671715)
Xu S, Hayashi Y, Takagishi Y, Itoh M, Murata Y.
Reduced polyalanine-expanded Arx mutant protein in developing mouse subpallium alters Lmo1 transcriptional regulation. Hum Mol Genet. (2013).(24122442)
Lee K, Mattiske T, Kitamura K, Gecz J, Shoubridge C.

RBRC03655, 129-Arx<tm(GCG)Kki>/7-2Rbrc

RBRC No. RBRC03655
Type Targeted MutationCartagena
Species Mus musculus
Strain name 129-Arx<tm(GCG)Kki>/7-2Rbrc
Former Common name Arx (GCG)7-2 KI (129)
H-2 Haplotype No Data
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Kunio Kitamura, Mitsubishi Kagaku Institute of Life Sciences. CCE/EK.CCE ES cells were used to generate the mutant mice.
Strain description Arx GCG 7 repeats knock-in mice. 7 repeats of the GCG triple were inserted at the codon 330 of Arx gene. C57BL/6 mixed background (RBRC03654 and RBRC03656), 129 background (RBRC03655), C3H mixed background (RBRC03657), ICR mixed background (RBRC03665).
Colony maintenance Heterozygote x Wild-type
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Arx
Symbol name aristaless related homeobox
Chromosome X
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name SV40 polyA signal
Chromosome X
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome X
Common name No Data
Symbol description No Data
References No Data

Research applications Mouse Models for Human Disease,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The BIOLOGICAL RESOURCE shall be used only for academic research purposes by
a non-profit organization.
The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and
negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL
RESOURCE for purposes of other than academic research.
In publishing the research results to be obtained by use of the BIOLOGICAL
RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the
source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi
Chemical Corporation.
The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical
Corporation of RECIPIENT organization, RECIPIENT name, the specific research
purpose and the date of distribution.
Additional information
1 No Data
Depositor Kitamura, Kunio (Mitsubishi Chemical Corporation) Kitamura, Kunio
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC03656, B6;129-Arx<tm(GCG)Kki>/7-2Rbrc

RBRC No. RBRC03656
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129-Arx<tm(GCG)Kki>/7-2Rbrc
Former Common name Arx (GCG)7-2KI (B6)
H-2 Haplotype No Data
ES cell line CCE/EK.CCE [129S/SvEv-Gpi1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Kunio Kitamura, Mitsubishi Kagaku Institute of Life Sciences. CCE/EK.CCE ES cells were used to generate the mutant mice.
Strain description Arx GCG 7 repeats knock-in mice. 7 repeats of the GCG triple were inserted at the codon 330 of Arx gene. C57BL/6 mixed background (RBRC03654 and RBRC03656), 129 background (RBRC03655), C3H mixed background (RBRC03657), ICR mixed background (RBRC03665).
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Arx
Symbol name aristaless related homeobox
Chromosome X
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name SV40 polyA signal
Chromosome X
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome X
Common name No Data
Symbol description No Data
References

Research applications Mouse Models for Human Disease,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The BIOLOGICAL RESOURCE shall be used only for academic research purposes by
a non-profit organization.
The RECIPIENT of a non-profit organization must prior contact RIKEN BRC and
negotiate with Mitsubishi Chemical Corporation to use the BIOLOGICAL
RESOURCE for purposes of other than academic research.
In publishing the research results to be obtained by use of the BIOLOGICAL
RESOURCE in relevant journals and meetings, the RECIPIENT must stipulate the
source of the BIOLOGICAL RESOURCE and an acknowledgment to Mitsubishi
Chemical Corporation.
The RECIPIENT agrees that RIKEN BRC informs annually to Mitsubishi Chemical
Corporation of RECIPIENT organization, RECIPIENT name, the specific research
purpose and the date of distribution.
Additional information
1 No Data
Depositor Kitamura, Kunio (Mitsubishi Chemical Corporation) Kitamura, Kunio
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months