RBRC02739, C57BL/6J-Tg(Dsg3TCR164)3

RBRC No. RBRC02739
Type TransgeneCartagena wks
Species Mus musculus
Strain name C57BL/6J-Tg(Dsg3TCR164)3
Former Common name Dsg3T3
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Tcra
Symbol name T-cell receptor alpha chain
Chromosome No Data
Common name No Data
Symbol description No Data
2 Symbol Tcrb
Symbol name T-cell receptor beta chain
Chromosome No Data
Common name No Data
Symbol description No Data
References J Immunol Methods. 1995 Mar 27;180(2):273-80.

Cassette vectors directing expression of T cell receptor genes in transgenic mice.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Amagai, Masayuki (Keio University) Amagai, Masayuki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05850, B6.Cg-Flg<tm1Amag>/Rbrc

RBRC No. RBRC05850
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Flg<tm1Amag>/Rbrc
Former Common name B6-FLG KO, B6-Flg KO, B6.Cg-Flg<tm1>
H-2 Haplotype No Data
ES cell line iTL BA1[(C57BL/6N x 129S6/SvEvTac)F1]
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Akiharu Kubo and Masayuki Amagai, Keio University School of Medicine in 2008. BA1 ES cells were used. The mutant mice were backcrossed to C57BL/6J by using speed congenic method (100 makers were replaced to C57BL/6 type). C57BL/6J background (RBRC05850), BALB/c (RBRC05851).
Strain description Flg, filaggrin gene knockout mice. The start codon in exon 2 and in-frame ATG sequences of exon 3 were replaced with a PGK-neo cassette. Homozygous mutant mice are viable, but exhibit dry scaly skin.
Colony maintenance Heterozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Flg
Symbol name filaggrin
Chromosome 3
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 3
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 3
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 3
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 3
Common name neo; neomycin;
Symbol description No Data
References

Research applications Dermatology Research,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT is requested to submit the abstract of the research project to the DEPOSITOR, and shall agree to decide the co-authorship of the DEPOSITOR based agreement with the DEPOSITOR in the case of any publications based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Mouse of the Month Sep 2013
2 Genotyping protocol <PCR>
Depositor Amagai, Masayuki (Keio University) Amagai, Masayuki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC05851, C.Cg-Flg<tm1>

RBRC No. RBRC05851
Type Targeted MutationCartagena
Species Mus musculus
Strain name C.Cg-Flg<tm1>
Former Common name BALB-FLG KO, BALB-Flg KO
H-2 Haplotype No Data
ES cell line iTL BA1[(C57BL/6N x 129S6/SvEvTac)F1]
Background strain BALB/cAJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Akiharu Kubo and Masayuki Amagai, Keio University School of Medicine in 2008. BA1 ES cells were used. The mutant mice were backcrossed to BALB/c by using speed congenic method (99.4% replacement). C57BL/6J background (RBRC05850), BALB/c (RBRC05851).
Strain description Flg, filaggrin gene knockout mice. The start codon in exon 2 and in-frame ATG sequences of exon 3 were replaced with a PGK-neo cassette. Homozygous mutant mice are viable, but exhibit dry scaly skin.
Colony maintenance Heterozygote x Homozygote [or Crossing to BALB/cAJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Flg
Symbol name filaggrin
Chromosome 3
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 3
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 3
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 3
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 3
Common name neo; neomycin;
Symbol description No Data
References

Research applications Cre/loxP system,
Dermatology Research,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT is requested to submit the abstract of the research project to the DEPOSITOR, and shall agree to decide the co-authorship of the DEPOSITOR based agreement with the DEPOSITOR in the case of any publications based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Mouse of the Month Sep 2013
2 Genotyping protocol <PCR>
Depositor Amagai, Masayuki (Keio University) Amagai, Masayuki
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02252, C57BL/6-Tg(hK5-DsRed2/hK14)1

RBRC No. RBRC02252
Type TransgeneCartagena wks
Species Mus musculus
Strain name C57BL/6-Tg(hK5-DsRed2/hK14)1
Former Common name DsRed2hK14 Tg, C57BL/6
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter human Keratin 5 promoter
1 Symbol KRT14
Symbol name Keratin 14 (human)
Chromosome UN
Common name Cytokeratin 14, epidermolysis bullosa simplex, Dowling-Meara, Koebner, K14, Krt-1.14, Krt1-14
Symbol description No Data
2 Symbol DsRed2
Symbol name Red Fluorescent Protein (Discosoma sp.)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Dermatology Research,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Amagai, Masayuki (Keio University) Amagai, Masayuki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02253, C57BL/6-Tg(hK5-mDsg3/EGFP)1

RBRC No. RBRC02253
Type TransgeneCartagena wks
Species Mus musculus
Strain name C57BL/6-Tg(hK5-mDsg3/EGFP)1
Former Common name mDsg3EGFP Tg, C57BL/6
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter human Keratin 5 promoter
2 Symbol Dsg3
Symbol name desmoglein 3
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Cell Biology Research,
Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Amagai, Masayuki (Keio University) Amagai, Masayuki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02731, C57BL/6J-Tg(Dsg3TCR140)1

RBRC No. RBRC02731
Type TransgeneCartagena wks
Species Mus musculus
Strain name C57BL/6J-Tg(Dsg3TCR140)1
Former Common name Dsg3H1
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Tcra
Symbol name T-cell receptor alpha chain
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol Tcrb
Symbol name T-cell receptor beta chain
Chromosome UN
Common name No Data
Symbol description No Data
References J Immunol Methods. 1995 Mar 27;180(2):273-80.

Cassette vectors directing expression of T cell receptor genes in transgenic mice.
Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Amagai, Masayuki (Keio University) Amagai, Masayuki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02732, C57BL/6J-Tg(Dsg3TCR140)2

RBRC No. RBRC02732
Type TransgeneCartagena wks
Species Mus musculus
Strain name C57BL/6J-Tg(Dsg3TCR140)2
Former Common name Dsg3H2
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Tcra
Symbol name T-cell receptor alpha chain
Chromosome No Data
Common name No Data
Symbol description No Data
2 Symbol Tcrb
Symbol name T-cell receptor beta chain
Chromosome No Data
Common name No Data
Symbol description No Data
References J Immunol Methods. 1995 Mar 27;180(2):273-80.

Cassette vectors directing expression of T cell receptor genes in transgenic mice.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP (Japanese)
Depositor Amagai, Masayuki (Keio University) Amagai, Masayuki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02734, C57BL/6J-Tg(Dsg3TCR162)1

RBRC No. RBRC02734
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6J-Tg(Dsg3TCR162)1
Former Common name Dsg3M1
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Tcra
Symbol name T-cell receptor alpha chain
Chromosome No Data
Common name No Data
Symbol description No Data
2 Symbol Tcrb
Symbol name T-cell receptor beta chain
Chromosome No Data
Common name No Data
Symbol description No Data
References J Immunol Methods. 1995 Mar 27;180(2):273-80.

Cassette vectors directing expression of T cell receptor genes in transgenic mice.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP (Japanese)
Depositor Amagai, Masayuki (Keio University) Amagai, Masayuki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02736, C57BL/6J-Tg(Dsg3TCR162)3

RBRC No. RBRC02736
Type TransgeneCartagena wks
Species Mus musculus
Strain name C57BL/6J-Tg(Dsg3TCR162)3
Former Common name Dsg3M3
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Tcra
Symbol name T-cell receptor alpha chain
Chromosome No Data
Common name No Data
Symbol description No Data
2 Symbol Tcrb
Symbol name T-cell receptor beta chain
Chromosome No Data
Common name No Data
Symbol description No Data
References J Immunol Methods. 1995 Mar 27;180(2):273-80.

Cassette vectors directing expression of T cell receptor genes in transgenic mice.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Amagai, Masayuki (Keio University) Amagai, Masayuki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02737, C57BL/6J-Tg(Dsg3TCR164)1

RBRC No. RBRC02737
Type TransgeneCartagena wks
Species Mus musculus
Strain name C57BL/6J-Tg(Dsg3TCR164)1
Former Common name Dsg3T1
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Tcra
Symbol name T-cell receptor alpha chain
Chromosome No Data
Common name No Data
Symbol description No Data
2 Symbol Tcrb
Symbol name T-cell receptor beta chain
Chromosome No Data
Common name No Data
Symbol description No Data
References J Immunol Methods. 1995 Mar 27;180(2):273-80.

Cassette vectors directing expression of T cell receptor genes in transgenic mice.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Amagai, Masayuki (Keio University) Amagai, Masayuki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months