RBRC02406, B6129-4930583H14Rik<tm1.1Hmd>

RBRC No. RBRC02406
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6129-4930583H14Rik<tm1.1Hmd>
Former Common name 4930583H14Rik null
H-2 Haplotype No Data
ES cell line G4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takako Maeda and Hiroshi Hamada, Institute for Molecular and Cellular Biology, Osaka University in 2007. G4 ES cells derived from (129S6 x C57BL/6)F1 were used. C57BL/6 and 129 mixed background.
Strain description 4930583H14Rik knockout mice. The ORF region of exon 4 flanked by loxP sites was removed by crossing with CAG-Cre mice (B6).
Health Report
Gene Details
Promoter No Data
1 Symbol 4930583H14Rik
Symbol name RIKEN cDNA 4930583H14 gene
Chromosome 3
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 3
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC03829, STOCK Tg(Lefty1 9.5-lacZ)F32Hmd

RBRC No. RBRC03829
Type TransgeneCartagena
Species Mus musculus
Strain name STOCK Tg(Lefty1 9.5-lacZ)F32Hmd
Former Common name Lefty1-9.5lacZ(F32)
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome No Data
Common name No Data
Symbol description No Data
References

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04718, B6;129B6-Cluap1<tm1Hmd>

RBRC No. RBRC04718
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129B6-Cluap1<tm1Hmd>
Former Common name Qilin (flox/+)
H-2 Haplotype No Data
ES cell line G4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Yanick Botlde and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University. The construct was electroporated into G4 ES cells derived from B6/129. Correctly targeted ES cells were aggregated with ICR molura. The resulting chimeric animals were crossed to C57BL/6 and then crossed to CAG-FLPe mice.
Strain description Cluap1 (Qilin) gene floxed mice. Exons 1 to 3 were flanked by loxP sites.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Cluap1
Symbol name clusterin associated protein 1
Chromosome 16
Common name Qilin
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 16
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 16
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05060, B6;B6C3(129)-Tg(Lefty1-cre)2Hmd

RBRC No. RBRC05060
Type TransgeneCartagena
Species Mus musculus
Strain name B6;B6C3(129)-Tg(Lefty1-cre)2Hmd
Former Common name Lefty1-cre/BAC (2) B6-Lefty1-cre Tg#2
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroshi Hamada, Institute for Molecular and Cellular Biology, Osaka University in 2008. The transgene was injected into the pronuclei of B6C3F1 fertilized egg.
Strain description No Data
Colony maintenance Carrier x Carrier, Noncarrier [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter mouse left right determination factor 1 promoter
1 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06203, B6.Cg-Pitx2<tm1Hmd>

RBRC No. RBRC06203
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Pitx2<tm1Hmd>
Former Common name Pitx2 A-17 flox, B6-Pitx2 floxed
H-2 Haplotype No Data
ES cell line G4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hidetaka Shiratori and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2001. G4 ES cells were used. The transgene was injected into the pronuclei of B6C3F1 fertilized eggs. C57BL/6 and 129 mixed background.
Strain description Pitx2 gene floxed mice. A loxP site was inserted in the intron 5, and a neo cassette with a 3' loxP site was inserted downstream of intron 5. Homozygous mice are viable and fertile.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Pitx2
Symbol name paired-like homeodomain transcription factor 2
Chromosome 3
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 3
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 3
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC06550, B6.Cg-Nodal<tm3.2Hmd>

RBRC No. RBRC06550
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Nodal<tm3.2Hmd>
Former Common name NodalΔRAREn, B6-Nodal/RAREn KO
H-2 Haplotype No Data
ES cell line G4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Masayuki Uehara and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University in 2009. G4 ES cells derived from (129 x C57BL/6)F1 were used to generate the mutant mice. Flanked REREn was removed by crossing with CAG-Cre mice (B6/129 background). A frt flanked PGK-neo cassette was removed by crossing with CAG-FLPe mice (B6/129 background). C57BL/6 and 129 mixed background.
Strain description REREn in intron 1 of the Nodal gene was removed. Homozygous mutant mice show no obvious abnormality.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 10
Common name No Data
Symbol description No Data
2 Symbol Nodal
Symbol name Nodal
Chromosome 10
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 10
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
Additional information
1 No Data
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC01229, B6;129-Foxh1<tm1.1Hmd>/HmdRbrc

RBRC No. RBRC01229
Type Targeted Mutation and Chromosomal Aberration and F2 HybridCartagena
Species Mus musculus
Strain name B6;129-Foxh1<tm1.1Hmd>/HmdRbrc
Former Common name B6;129-FoxH1+/-, FAST+/-, B6;129-FoxH1tm1Hh(B6;129-FoxH1+/-,FAST+/- ) knockout mice
H-2 Haplotype No Data
Donor strain (129X1/SvJ x 129S1/Sv)F1-Kitl+ via R1 ES cell line
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Drs. Masamichi Yamamoto and Hiroshi Hamada at Osaka University in 2001. Foxh1<tm1.1Hmd> mutant mice were generated from Foxh1<tm1Hmd> mutant mice by crossing with cre-expressing line, Tg(ACTB-cre)Miya mice strain.
Strain description B6;129-Foxh1<tm1.1Hmd>. The transcription factor Foxh1 (horkhead box H1) mediates signaling by transforming growth factor-β, Activin, and Nodal. Foxh1 null deficient mice are perinatal lethal. The embryos of Foxh1 deficient mice exhibit various patterning defects by the loss of Nodal signaling. This mice are useful for the study of Foxh1 gene in vivo.
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6Cr)
Homozygous mutant mice are perinatal lethal.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol FoxH1
Symbol name forkhead box H1
Chromosome 15
Common name FAST
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 15
Common name No Data
Symbol description No Data
References Genes Dev. 2001 May 15;15(10):1242-56.

The transcription factor FoxH1 (FAST) mediates Nodal signaling during anterior-posterior patterning and node formation in the mouse.
Research applications Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on
use of it from the DEPOSITOR.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02407, B6129-Lplunc1<tm1Hmd>

RBRC No. RBRC02407
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6129-Lplunc1<tm1Hmd>
Former Common name L-plunc1 knockout
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol U46068
Symbol name cDNA sequence U46068
Chromosome 2
Common name 2310039C12Rik, LPlunc1, von Ebner minor salivary protein
Symbol description No Data
2 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 2
Common name internal ribosomal entry site from EMCV
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 2
Common name No Data
Symbol description No Data
4 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 2
Common name No Data
Symbol description No Data
5 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 2
Common name No Data
Symbol description E. coli beta galactosidase
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 2
Common name No Data
Symbol description No Data
7 Symbol poly A
Symbol name poly A
Chromosome 2
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
8 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 2
Common name neo; neomycin;
Symbol description No Data
References

Research applications Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
The BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC03830, STOCK Tg(Pitx2 17k-lacZ)26Hmd

RBRC No. RBRC03830
Type TransgeneCartagena
Species Mus musculus
Strain name STOCK Tg(Pitx2 17k-lacZ)26Hmd
Former Common name Pitx2 17-LacZ(26)
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name No Data
Chromosome No Data
Common name No Data
Symbol description No Data
References Development. 2006 Aug;133(15):3015-25.

Conserved regulation and role of Pitx2 in situs-specific morphogenesis of visceral organs.
Conserved regulation and role of Pitx2 in situs-specific morphogenesis of visceral organs.
Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04719, B6;129B6-Cluap1<tm1.1Hmd>

RBRC No. RBRC04719
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129B6-Cluap1<tm1.1Hmd>
Former Common name Qilin (null/+)
H-2 Haplotype No Data
ES cell line G4 [(129S6/SvEvTac x C57BL/6NCr)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Yanick Botlde and Hiroshi Hamada, Graduate School of Frontier Biosciences, Osaka University. The construct was electroporated into G4 ES cells derived from B6/129. Correctly targeted ES cells were aggregated with ICR molura. The resulting chimeric animals were crossed to C57BL/6 and then crossed to CAG-fLPe and CAG-Cre mice.
Strain description Cluap1 (Qilin) gene knockout mice. Exons 1 to 3 were removed. Homozygous mutant mice are embryonic lethal.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Cluap1
Symbol name clusterin associated protein 1
Chromosome 16
Common name Qilin
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 16
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 16
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Hamada, Hiroshi (RIKEN Center for Developmental Biology) Hamada, Hiroshi
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months