RBRC01477, B6.129S6-Ptprj<tm1Taka>/SataRbrc

RBRC No. RBRC01477
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129S6-Ptprj<tm1Taka>/SataRbrc
Former Common name CD148/DEP-1 KO/PTPRJ, CD148 KO
H-2 Haplotype No Data
Donor strain 129S6/SvEvTac via TL1/TL-1 ES cell line
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr Takamune Takahashi at Vanderbilt University Medical Center in 2003. A targeting construct containing EGFP and neo was transfered into ES cells to disrupt Ptprj gene. GFP fluorescence was not detected in mutant mice.
Strain description B6.129S6-Ptprj<tm1Taka>. Protein tyrosine phospatase, receptor type J (CD148) is a member of the receptor tyrosine phosphatase family playing an important role in cell growth, differentiation, and development. Homozygous Ptprj<tm1Taka> mutant mice were embryonic lethal due to abnormal cardiovascular system morphology.
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Ptprj
Symbol name protein tyrosine phosphatase, receptor type, J
Chromosome 2
Common name BET, Byp, CD148, DEP-1, Ptpb2, PTPbeta2, Scc-1, Scc1
Symbol description No Data
2 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome 2
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 2
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 2
Common name neo; neomycin;
Symbol description No Data
References Mol Cell Biol. 2003 Mar;23(5):1817-31.

A mutant receptor tyrosine phosphatase, CD148, causes defects in vascular development.
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mol. Cell. Biol., 23, 1817-1831 (2003).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Satake, Masanobu (Institute of Development, Aging and Cancer, Tohoku University) Satake, Masanobu
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01478, B6.129P2-Ptprt<tm1Sata>/Rbrc

RBRC No. RBRC01478
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Ptprt<tm1Sata>/Rbrc
Former Common name PTP rho-1 KO/PTPRT, PTP rho KO 233
H-2 Haplotype No Data
Donor strain 129P2/OlaHsd via E14 ES cell line
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Masanobu Satake, Tohoku University. C57BL/6 background.
Strain description PTP rho, Protein tyrosine phospatase receptor type T, is a member of the receptor tyrosine phosphatase family. PTPs are signaling molecules that regulate a variety of cellular processes including mitotic cycle, cell growth, differentiation, and oncogenic transformation. At present, homozygous mutant mice show no abnormality.
Colony maintenance backcross to C57BL/6 (Heterozygote x C57BL/6J)
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Ptprt
Symbol name protein tyrosine phosphatase, receptor type, T
Chromosome 2
Common name RPTPrho
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 2
Common name neo; neomycin;
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Satake, Masanobu (Institute of Development, Aging and Cancer, Tohoku University) Satake, Masanobu
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01479, B6.129S4-Cbfb<tm1Tno>/SataRbrc

RBRC No. RBRC01479
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129S4-Cbfb<tm1Tno>/SataRbrc
Former Common name PEBP1beta/CBFbeta KO
H-2 Haplotype No Data
Donor strain 129S4/SvJae via J1 ES cell line
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Cbfb
Symbol name core binding factor beta
Chromosome 8
Common name PEA2, Pebp2, PEBP2b, Pebpb2
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 8
Common name neo; neomycin;
Symbol description No Data
References Proc Natl Acad Sci U S A 1997 May 27;94(11):5697-702.

Hematopoiesis in the fetal liver is impaired by targeted mutagenesis of a gene encoding a non-DNA binding subunit of the transcription factor, polyomavirus enhancer binding protein 2/core binding factor.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Proc. Natl. Acad. Sci., 94 5697-5702 (1997).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Satake, Masanobu (Institute of Development, Aging and Cancer, Tohoku University) Satake, Masanobu
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01489, B6.129P2-Ptprt<tm1Sata>/SataRbrc

RBRC No. RBRC01489
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Ptprt<tm1Sata>/SataRbrc
Former Common name PTP rho KO 231
H-2 Haplotype No Data
ES cell line E14.1 [129P2/OlaHsd]
Donor strain 129P2/OlaHsd via E14 ES cell line
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Masanobu Satake, Tohoku University. C57BL/6 background.
Strain description PTP rho, Protein tyrosine phospatase receptor type T, is a member of the receptor tyrosine phosphatase family. PTPs are signaling molecules that regulate a variety of cellular processes including mitotic cycle, cell growth, differentiation, and oncogenic transformation. At present, homozygous mutant mice show no abnormality.
Colony maintenance backcross to C57BL/6 (Heterozygote x C57BL/6J)
Health Report
Gene Details
Promoter No Data
1 Symbol Ptprt
Symbol name protein tyrosine phosphatase, receptor type, T
Chromosome 2
Common name RPTPrho
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 2
Common name neo; neomycin;
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Satake, Masanobu (Institute of Development, Aging and Cancer, Tohoku University) Satake, Masanobu
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
PTPRT regulates high-fat diet-induced obesity and insulin resistance. PLoS One. (2014).(24949727)
Feng X, Scott A, Wang Y, Wang L, Zhao Y, Doerner S, Satake M, Croniger CM, Wang Z.

RBRC01450, B6.129S4-Runx1<tm1Sata>/SataRbrc

RBRC No. RBRC01450
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129S4-Runx1<tm1Sata>/SataRbrc
Former Common name AML1 KO, Runx1 KO mice
H-2 Haplotype No Data
Donor strain 129S4/SvJae
Background strain C57BL/6JJcl
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Masanobu Satake, Tohoku University in 1998. A targeting vector containing pgk-neo cassette was transfered into ES cells to relpace exons 4.
Strain description B6.129S4-Runx1<tm1Sata>. Runx1, runt related transcription factor 1 (also called AML1) encodes the DNA binding subunit of the heterodimeric transcription factor. Runx1 is one of the most frequent targets of chromosome translocation associated with several subtypes of human acute myeloid or lymphoblastic leukemia. Mice homozygous for the Runx1 null mutantion die embryonic day 12.5 due to massive hemorrhage in the central nervous system. In fetal livers of mutant embryos, no hematopoieteic cells are generated.
Colony maintenance Backcross to C57BL/6 (Heterozygote x C57BL/6JJcl)
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Runx1
Symbol name runt related transcription factor 1
Chromosome 16
Common name AML1, Cbfa2, Pebp2a2, runt domain, alpha subunit 2
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 16
Common name neo; neomycin;
Symbol description No Data
References Oncogene. 1998 Nov 5;17(18):2287-93.

AML1(-/-) embryos do not express certain hematopoiesis-related gene transcripts including those of the PU.1 gene.
Research applications Cancer Research,
Cell Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Oncogene, 17, 2287-2293 (1998).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use
of it from the DEPOSITOR to avoid possible conflicts with DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a
citation of the following literature designated by the DEPOSITOR is requested.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE,
an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to include the DEPOSITOR as a co-author in any publications
resulted by the use of the BIOLOGICAL RESOURCE during the first 2 years after deposition by the DEPOSITOR to the RIKEN BRC.
The RECIPIENT should contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Satake, Masanobu (Institute of Development, Aging and Cancer, Tohoku University) Satake, Masanobu
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months