RBRC01893, B6.Cg-Mgl2<tm1Tats>/TatsRbrc

RBRC No. RBRC01893
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.Cg-Mgl2<tm1Tats>/TatsRbrc
Former Common name mMgl2 knockout mice (C57BL/6J)
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain C57BL/6J
Appearance
1 Appearance No Data
Genotype No Data
Strain development eveloped by Kaori Denda and Tasturo Irimura, Graduate School of Pharmaceutical Sciences, The University of Tokyo. TT2 ES cells derived from B6CBAF1 were used to generate the knockout mice. The mice were backcrossed to C57BL/6J and BALB/c, respectively (RBRC01893 and RBRC01894).
Strain description Mgl2 gene knockout mice. Exon 2 of the Mgl2 was replaced with a PGK-neo cassette. The MGL is one of the calcium-dependent (C type) lectins, which are intrinsic carbohydrate recognition proteins. Homozygous mutant mice are viable and fertile. Under investigation (details unknown). C57BL/6 background (RBRC01893) and BALB/c background (RBRC01894).
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
Health Report
Gene Details
Promoter No Data
1 Symbol Mgl2
Symbol name macrophage galactose N-acetyl-galactosamine specific lectin 2
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 11
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Irimura, Tatsuro (The University of Tokyo) Irimura, Tatsuro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC01894, C.Cg-Mgl2<tm1Tats>/TatsRbrc

RBRC No. RBRC01894
Type Targeted MutationCartagena
Species Mus musculus
Strain name C.Cg-Mgl2<tm1Tats>/TatsRbrc
Former Common name mMgl2 knockout mice (BALB/c)
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain BALB/cAnNCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain development eveloped by Kaori Denda and Tasturo Irimura, Graduate School of Pharmaceutical Sciences, The University of Tokyo. TT2 ES cells derived from B6CBAF1 were used to generate the knockout mice. The mice were backcrossed to C57BL/6J and BALB/c, respectively (RBRC01893 and RBRC01894).
Strain description Mgl2 gene knockout mice. Exon 2 of the Mgl2 was replaced with a PGK-neo cassette. The MGL is one of the calcium-dependent (C type) lectins, which are intrinsic carbohydrate recognition proteins. Homozygous mutant mice are viable and fertile. Under investigation (details unknown). C57BL/6 background (RBRC01893) and BALB/c background (RBRC01894).
Colony maintenance Homozygote x Homozygote [or Crossing to BALB/cAnNCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol Mgl2
Symbol name macrophage galactose N-acetyl-galactosamine specific lectin 2
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 11
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Genetic Background
2 Lab HP
3 Genotyping protocol <PCR>
Depositor Irimura, Tatsuro (The University of Tokyo) Irimura, Tatsuro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC03898, B6;B6CB-Mucl3<tm1Tats>/Tats

RBRC No. RBRC03898
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6;B6CB-Mucl3<tm1Tats>/Tats
Former Common name Muc21 KO, B6;B6CB-Muc21<tm1Tats>/Tats
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Mucl3
Symbol name mucin like 3
Chromosome 17
Common name No Data
Symbol description No Data
2 Symbol lox2272
Symbol name No Data
Chromosome 17
Common name No Data
Symbol description No Data
3 Symbol lox71
Symbol name No Data
Chromosome 17
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 17
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 17
Common name No Data
Symbol description No Data
References Glycobiology. 2008 Jan;18(1):74-83. Epub 2007 Oct 31.

Identification and expression of human epiglycanin/MUC21: a novel transmembrane mucin.
Research applications Cancer Research,
Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
後日指定
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Irimura, Tatsuro (The University of Tokyo) Irimura, Tatsuro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05439, C57BL/6J-Muc21<tm2(Venus)Tats>/1

RBRC No. RBRC05439
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6J-Muc21<tm2(Venus)Tats>/1
Former Common name C57BL/6J-Muc21/1TatsRbrc
H-2 Haplotype No Data
ES cell line B6J-S1 [C57BL/6J]
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Tatsuro Irimura, Graduate School of Pharmaceutical Sciences, The University of Tokyo in 2011. C57BL/6J background. Main line (RBRC05439), Subline (RBRC05440).
Strain description Mucin 21/epiglycanin venus knock-in mice. A PGK-neo cassette and venus gene was inserted into Muc21 gene. Homozygous mutant mice show no obvious abnormality.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Gm9573
Symbol name predicted gene 9573
Chromosome 17
Common name No Data
Symbol description No Data
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome No Data
Common name neo; neomycin;
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome No Data
Common name No Data
Symbol description No Data
4 Symbol Venus
Symbol name Green Fluorescent Protein, F46L (Jellyfish)
Chromosome No Data
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome No Data
Common name No Data
Symbol description No Data
References Glycobiology. 2008 Jan;18(1):74-83. Epub 2007 Oct 31.

Identification and expression of human epiglycanin/MUC21: a novel transmembrane mucin.
Identification and expression of human epiglycanin/MUC21: a novel transmembrane mucin.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
(後日指定)
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Irimura, Tatsuro (The University of Tokyo) Irimura, Tatsuro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05440, C57BL/6J-Muc21<tm2(Venus)Tats>/2

RBRC No. RBRC05440
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6J-Muc21<tm2(Venus)Tats>/2
Former Common name C57BL/6J-Muc21/2TatsRbrc
H-2 Haplotype No Data
ES cell line B6J-S1 [C57BL/6J]
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Tatsuro Irimura, Graduate School of Pharmaceutical Sciences, The University of Tokyo in 2011. C57BL/6J background. Main line (RBRC05439), Subline (RBRC05440).
Strain description Mucin 21/epiglycanin venus knock-in mice. A PGK-neo cassette and venus gene was inserted into Muc21 gene. Homozygous mutant mice show no obvious abnormality.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome No Data
Common name neo; neomycin;
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome No Data
Common name No Data
Symbol description No Data
3 Symbol Venus
Symbol name Green Fluorescent Protein, F46L (Jellyfish)
Chromosome No Data
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome No Data
Common name No Data
Symbol description No Data
References Glycobiology. 2008 Jan;18(1):74-83. Epub 2007 Oct 31.

Identification and expression of human epiglycanin/MUC21: a novel transmembrane mucin.
Identification and expression of human epiglycanin/MUC21: a novel transmembrane mucin.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
(後日指定)
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
Depositor Irimura, Tatsuro (The University of Tokyo) Irimura, Tatsuro
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05713, B6.129S7-Asgr1<tm1Ishi>/Tats

RBRC No. RBRC05713
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129S7-Asgr1<tm1Ishi>/Tats
Former Common name Asgr1 KO
H-2 Haplotype No Data
ES cell line JH1 [129S7/SvEvBrd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Shun Ishibashi, Faculty of Medicine, University of Tokyo in 2001. JH1 ES cells derived from 129S7/SvEvBrd were used for the generation of knockout mice. The mice were backcrossed to C57BL/6J for 8 generations by Laboratory of Cancer Biology and Molecular Immunology, University of Tokyo.
Strain description Asgr1 knockout mice. The transcription initiation codon and the sequences encoding the transmembrane domain of Asgr1 gene were replaced with a pol2neo cassette. Homozygous mice show an impaired hepatic clearance of asialoglycoproteins.
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Asgr1
Symbol name asialoglycoprotein receptor 1
Chromosome 11
Common name No Data
Symbol description No Data
References

Research applications Cell Biology Research,
Metabolism Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Biol. Chem., 276, 12624-12628 (2001).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 No Data
Depositor Irimura, Tatsuro (The University of Tokyo) Irimura, Tatsuro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01889, B6.Cg-Clec10a<tm1Tats>/TatsRbrc

RBRC No. RBRC01889
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Clec10a<tm1Tats>/TatsRbrc
Former Common name mMgl1 lacZ knock in mice (C57BL/6J)
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain C57BL/6J
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Kaori Denda and Tasturo Irimura, Graduate School of Pharmaceutical Sciences, The University of Tokyo. TT2 ES cells derived from B6CBAF1 were used to generate the knockout mice. RBRC01889: backcrossed to C57BL/6J. RBRC01890: BALB/c semicongenic (BALB/c, C57BL/6 and BALB/c mixed).
Strain description Mgl1 gene knockout mice. Exons 2 to 10 were replaced with a PGK-neo and lacZ cassette. The MGL is one of the calcium-dependent (C type) lectins, which are intrinsic carbohydrate recognition proteins. C57BL/6 background (RBRC01889) and BALB/c mixed background (RBRC01890). Under investigation (details unknown).
Colony maintenance Heterozygote x Wild-type [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Clec10a
Symbol name macrophage galactose N-acetyl-galactosamine specific lectin 1
Chromosome 11
Common name Mgl1
Symbol description No Data
2 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 11
Common name No Data
Symbol description No Data
3 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 11
Common name No Data
Symbol description E. coli beta galactosidase
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
Depositor Irimura, Tatsuro (The University of Tokyo) Irimura, Tatsuro
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01890, C.Cg-Clec10a<tm1Tats>/TatsRbrc

RBRC No. RBRC01890
Type Targeted MutationCartagena
Species Mus musculus
Strain name C.Cg-Clec10a<tm1Tats>/TatsRbrc
Former Common name mMgl1 lacZ knock in mice (BALB/c)
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain BALB/cAnNCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Kaori Denda and Tasturo Irimura, Graduate School of Pharmaceutical Sciences, The University of Tokyo. TT2 ES cells derived from B6CBAF1 were used to generate the knockout mice. RBRC01889: backcrossed to C57BL/6J. RBRC01890: BALB/c semicongenic (BALB/c, C57BL/6 and BALB/c mixed).
Strain description Mgl1 gene knockout mice. Exons 2 to 10 were replaced with a PGK-neo and lacZ cassette. The MGL is one of the calcium-dependent (C type) lectins, which are intrinsic carbohydrate recognition proteins. C57BL/6 background (RBRC01889) and BALB/c mixed background (RBRC01890). Under investigation (details unknown).
Colony maintenance Heterozygote x Wild-type [BALB/cAnNCrlCrlj]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Clec10a
Symbol name macrophage galactose N-acetyl-galactosamine specific lectin 1
Chromosome 11
Common name Mgl1
Symbol description No Data
2 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 11
Common name No Data
Symbol description No Data
3 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 11
Common name No Data
Symbol description E. coli beta galactosidase
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
Depositor Irimura, Tatsuro (The University of Tokyo) Irimura, Tatsuro
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01891, B6.129-Clec10a<tm1Hed>/TatsRbrc

RBRC No. RBRC01891
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129-Clec10a<tm1Hed>/TatsRbrc
Former Common name mMgl1 knockout mice (C57BL/6J)
H-2 Haplotype No Data
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain C57BL/6J
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Stephen M Hedrick, University of California. R1 ES cells were used to generate the knockout mice. R RBRC01891: backcrossed to C57BL/6J over 8 times. RBRC01892: backcrossed to C57BL/6J for 8 times, and then backcrossed to BALB/c over 6 times.
Strain description Mgl1 gene knockout mice. Exons 2 and 3 were replaced with a PGK-neo cassette. The MGL is one of the calcium-dependent (C type) lectins, which are intrinsic carbohydrate recognition proteins. C57BL/6 background (RBRC01891) and BALB/c mixed background (RBRC01892). Homozygous mutant mice were viable and fertile.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Clec10a
Symbol name macrophage galactose N-acetyl-galactosamine specific lectin 1
Chromosome 11
Common name Mgl1
Symbol description No Data
2 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 11
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References Mol Cell Biol. 2002 Jul;22(14):5173-81.

Generation of mice deficient for macrophage galactose- and N-acetylgalactosamine-specific lectin: limited role in lymphoid and erythroid homeostasis and evidence for multiple lectins.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Irimura, Tatsuro (The University of Tokyo) Irimura, Tatsuro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC01892, C.B6(129)-Clec10a<tm1Hed>/TatsRbrc

RBRC No. RBRC01892
Type Targeted MutationCartagena
Species Mus musculus
Strain name C.B6(129)-Clec10a<tm1Hed>/TatsRbrc
Former Common name mMgl1 knockout mice (BALB/c)
H-2 Haplotype No Data
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain BALB/cAnNCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Stephen M Hedrick, University of California. R1 ES cells were used to generate the knockout mice. R RBRC01891: backcrossed to C57BL/6J over 8 times. RBRC01892: backcrossed to C57BL/6J for 8 times, and then backcrossed to BALB/c over 6 times.
Strain description Mgl1 gene knockout mice. Exons 2 and 3 were replaced with a PGK-neo cassette. The MGL is one of the calcium-dependent (C type) lectins, which are intrinsic carbohydrate recognition proteins. C57BL/6 background (RBRC01891) and BALB/c mixed background (RBRC01892). Homozygous mutant mice were viable and fertile.
Colony maintenance Homozygote x Homozygote [or Crossing to BALB/cAnNCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol Clec10a
Symbol name macrophage galactose N-acetyl-galactosamine specific lectin 1
Chromosome 11
Common name Mgl1
Symbol description No Data
2 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 11
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
References Mol Cell Biol. 2002 Jul;22(14):5173-81.

Generation of mice deficient for macrophage galactose- and N-acetylgalactosamine-specific lectin: limited role in lymphoid and erythroid homeostasis and evidence for multiple lectins.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Irimura, Tatsuro (The University of Tokyo) Irimura, Tatsuro
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months