RBRC02293, B6.129P2-p130Cas<tm1.1Homy>

RBRC No. RBRC02293
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-p130Cas<tm1.1Homy>
Former Common name Cas-ex2<flox/flox>
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2003.
Strain description No Data
Colony maintenance backcross to C57BL/6N (Heterozygote x C57BL/6NCrlCrlj)
Health Report
Gene Details
Promoter No Data
1 Symbol Bcar1
Symbol name breast cancer anti-estrogen resistance 1
Chromosome 8
Common name Cas, Crkas, p130 Cas
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 8
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 8
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 8
Common name No Data
Symbol description No Data
References Genes Cells. 2008 Feb;13(2):145-57.

Functional analysis of Src homology 3-encoding exon (exon 2) of p130Cas in primary fibroblasts derived from exon 2-specific knockout mice.
Research applications Cell Biology Research,
Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05201, B6;129P2-Mbtd1<tm1Homy>

RBRC No. RBRC05201
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Mbtd1<tm1Homy>
Former Common name Hemp KO B6-Mbtd1 KI
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. The Mbtd1 mutant mice were generated by replacing the mbt-coding exons with IRES-GFP and floxed neo resistant gene. The neo resistant gene was removed by microinjecting a Cre expression vector into fertilized eggs. The mutant mice were backcrossed with C57BL/6N for at least 10 times.
Strain description Mbtd1 (Hemp) gene knockout mice. Exon 6, 7 and 8 (mbt-coding exons) were replaced with an IRES-GFP. Western blot analysis showed absence of Mbtb1 protein. Homozygous null mutant mice show skeletal abnormalities and are neonatal lethal.
Colony maintenance Heterozygote X Wild-type [C57BL/6NCrlCrlj]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Mbtd1
Symbol name mbt domain containing 1
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome No Data
Common name internal ribosomal entry site from EMCV
Symbol description No Data
3 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome No Data
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome No Data
Common name No Data
Symbol description No Data
References

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02294, B6.Cg-p130Cas<tm1.2Homy>

RBRC No. RBRC02294
Type Targeted Mutation CongenicCartagena wks
Species Mus musculus
Strain name B6.Cg-p130Cas<tm1.2Homy>
Former Common name Cas-ex2<+/->
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2003.
Strain description No Data
Colony maintenance backcross to C57BL/6N (Heterozygote x C57BL/6NCrlCrlj)
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Bcar1
Symbol name breast cancer anti-estrogen resistance 1
Chromosome 8
Common name Cas, Crkas, p130 Cas
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 8
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 8
Common name No Data
Symbol description No Data
References No Data

Research applications Cell Biology Research,
Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC05202, B6;129P2-Mbtd1<tm2Homy>

RBRC No. RBRC05202
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Mbtd1<tm2Homy>
Former Common name Hemp cKO B6-Mbtd1 floxed
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. A loxP was inserted upstream of exon 6 and an FRT flanked neo cassette with a 3' loxP site was inserted downstream of exon 5. Neo cassette was removed by crossing with FLPe mice (C57BL/6-Tg (CAG-FLPe) 36Ito). The mice were backcrossed with C57BL/6N for at least 7 times.
Strain description Mbtd1 (Hemp) gene floxed mice. Exon 6 of Mbtd1 gene is flanked by loxP sites. Conditional Mbtd1 deficient mice can be generated by crossing with tissue-specific Cre mice. Homozygous mice are viable and fertile.
Colony maintenance Heterozygote X Wild-type [C57BL/6NCrlCrlj]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Mbtd1
Symbol name mbt domain containing 1
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome No Data
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome No Data
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02295, B6.Cg-p130Cas<tm1.2Homy> Tg(BLG-cre)1Homy

RBRC No. RBRC02295
Type Targeted Mutation and TransgeneCartagena wks
Species Mus musculus
Strain name B6.Cg-p130Cas<tm1.2Homy> Tg(BLG-cre)1Homy
Former Common name Cas-ex2<flox/flox>-Tg(BLG-Cre)
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2003.
Strain description Casp130 (Bcar1) gene exon 2 floxed and BLG (beta lactoglobulin)-cre double mutant mice. Viable and fertile, but the reproductive performance is not so good.
Colony maintenance KO: Homozygote x Homozygote; Tg: Carrier x Carrier [or Crossing to C57BL/6NCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol Bcar1
Symbol name breast cancer anti-estrogen resistance 1
Chromosome 8
Common name Cas, Crkas, p130 Cas
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 8
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 8
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 8
Common name No Data
Symbol description No Data
Promoter sheep BLG promoter
5 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Cell Biology Research,
Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05203, C57BL/6-Tg(TH-ALK*F1174L)5Homy

RBRC No. RBRC05203
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(TH-ALK*F1174L)5Homy
Former Common name B6-Th-ALK/F1174L Tg#5L
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine and Junko Takita, The University of Tokyo Hospital. A F1174L mutant hALK gene under the TH promoter was injected into the pronuclei of C57BL/6N fertilized eggs.
Strain description Human ALK (anaplastic lymphoma kinase) gene having F1174L mutation transgenic mice. F1174L mutation was identified in a patient with an inflammatory myofibroblastic tumor harboring a RANBP2-ALK translocation.
Health Report
Gene Details
Promoter human tyrosine hydroxylase promoter
1 Symbol ALK
Symbol name human ALK cDNA (F1174L mutant)
Chromosome UN
Common name No Data
Symbol description No Data
References Nature. 2008 Oct 16;455(7215):971-4.

Oncogenic mutations of ALK kinase in neuroblastoma.
Research applications Cancer Research,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02296, BDF1-Tg(Crx-Gatad1)1Homy

RBRC No. RBRC02296
Type TransgeneCartagena wks
Species Mus musculus
Strain name BDF1-Tg(Crx-Gatad1)1Homy
Former Common name BDF1-Tg(Crx-ODAG)
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome UN
Common name internal ribosomal entry site from EMCV
Symbol description No Data
2 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol poly A
Symbol name SV40 poly A signal
Chromosome UN
Common name No Data
Symbol description No Data
Promoter Crx (cone-rod homeobox containing gene) promoter
4 Symbol Gatad1
Symbol name GATA zinc finger domain containing 1
Chromosome UN
Common name 2310031E19Rik, 2810047M21Rik, 8430439A17Rik, B330017N08Rik, Odag
Symbol description No Data
References Invest Ophthalmol Vis Sci. 2009 Jan;50(1):242-8. Epub 2008 Sep 12.

Elevated intraocular pressure, optic nerve atrophy, and impaired retinal development in ODAG transgenic mice.
Research applications Fluorescent Proteins/lacZ System,
Sensorineural Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05235, C57BL/6-Tg(TH-ALK*F1174L)9Homy

RBRC No. RBRC05235
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(TH-ALK*F1174L)9Homy
Former Common name B6-Th-ALK/F1174L Tg#9L
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine and Junko Takita, The University of Tokyo Hospital. A F1174L mutant hALK gene under the TH promoter was injected into the pronuclei of C57BL/6N fertilized eggs.
Strain description Human ALK (anaplastic lymphoma kinase) gene having F1174L mutation transgenic mice. F1174L mutation was identified in a patient with an inflammatory myofibroblastic tumor harboring a RANBP2-ALK translocation.
Health Report
Gene Details
Promoter human tyrosine hydroxylase promoter
1 Symbol ALK
Symbol name human ALK cDNA (F1174L mutant)
Chromosome UN
Common name No Data
Symbol description No Data
References Nature. 2008 Oct 16;455(7215):971-4.

Oncogenic mutations of ALK kinase in neuroblastoma.
Research applications Cancer Research,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02338, B6;129P2-p130Cas<tm2.1Homy>

RBRC No. RBRC02338
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6;129P2-p130Cas<tm2.1Homy>
Former Common name Cas-FL conditional KO
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Dr. Hiroaki Honda, Hiroshima University in 2005.
Strain description Bcar1 (p130Cas) is an adaptor molecule on growth factor receptor and integrin activation functions as regulator of cell motility and invasion. This conditional knockout mice have a loxP-flanked Bcar1 allele. Conditional Bcar1 deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenance backcross to C57BL/6N (Heterozygote x C57BL/6NCrlCrlj)
Health Report
Gene Details
Promoter No Data
1 Symbol Bcar1
Symbol name breast cancer anti-estrogen resistance 1
Chromosome 8
Common name Cas, Crkas, p130 Cas
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 8
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 8
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 8
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Skeletal muscle adaptation in response to mechanical stress in p130cas-/- mice. Am J Physiol Cell Physiol.304(6): C541-7 (2013).(23325412)
Akimoto T, Okuhira K, Aizawa K, Wada S, Honda H, Fukubayashi T, Ushida T.

RBRC05494, B6;129P2-Tnfaip3<tm1Homy>

RBRC No. RBRC05494
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Tnfaip3<tm1Homy>
Former Common name A20 cKO, B6-Tnfaip3 floxed
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6N mice.
Strain description A20 (Tnfaip3) gene floxed mice. Exon 3 of A20 gene was flanked by loxP sites. Conditional A20 deficient mice can be generated by crossing with tissue-specific Cre mice.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6NCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol Tnfaip3
Symbol name tumor necrosis factor, alpha-induced protein 3
Chromosome 10
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 10
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 10
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
2 Mouse of the Month May 2014
3 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months