RBRC03826, B6;B6D2-Tg(Emu-Zfp521)1Homy

RBRC No. RBRC03826
Type TransgeneCartagena
Species Mus musculus
Strain name B6;B6D2-Tg(Emu-Zfp521)1Homy
Former Common name E myu -Evi3
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Zfp521
Symbol name zinc finger protein 521
Chromosome 18
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05497, B6.129P2-Hepacam2<tm1Homy>

RBRC No. RBRC05497
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Hepacam2<tm1Homy>
Former Common name MIKI KO
H-2 Haplotype No Data
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2006. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6N mice.
Strain description Hepacam2 knockout mice. Exon 2 of Hepacam2 gene was replaced with a lacZ-frt-neo-frt cassette. Homozygous mutant mice are viable and fertile.
Colony maintenance Heterozygote x Wild-type [C57BL/6NCrlCrlj]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Hepacam2
Symbol name HEPACAM family member 2
Chromosome 6
Common name No Data
Symbol description No Data
References

Research applications Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04607, B6;129P2-Ciz1<tm1.1Homy>

RBRC No. RBRC04607
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Ciz1<tm1.1Homy>
Former Common name Ciz1 knockout
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome No Data
Common name neo; neomycin;
Symbol description No Data
2 Symbol Ciz1
Symbol name CDKN1A interacting zinc finger protein 1
Chromosome No Data
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome No Data
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome No Data
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05498, B6.129P2-Samd9l<tm1Homy>

RBRC No. RBRC05498
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Samd9l<tm1Homy>
Former Common name TITAN KO
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2006. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6N mice.
Strain description Samd9L knockout mice. A part of Exon 2 of Samd9L gene was replaced with a lacZ-loxP-neo-loxP cassette. Homozygous mutant mice are viable and fertile.
Colony maintenance Heterozygote x Wild-type [C57BL/6NCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol Samd9l
Symbol name sterile alpha motif domain containing 9-like
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name neo; neomycin;
Symbol description No Data
References

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04608, B6;129P2-Runx1<tm1Homy>

RBRC No. RBRC04608
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Runx1<tm1Homy>
Former Common name Runx1-delta IRES
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Runx1
Symbol name runt related transcription factor 1
Chromosome 16
Common name AML1, Cbfa2, Pebp2a2, runt domain, alpha subunit 2
Symbol description No Data
2 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome 16
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 16
Common name No Data
Symbol description No Data
4 Symbol Ires
Symbol name internal ribosomal entry site (EMCV)
Chromosome 16
Common name No Data
Symbol description No Data
5 Symbol RUNX1
Symbol name runt related transcription factor 1(human)
Chromosome 16
Common name No Data
Symbol description No Data
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 16
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05655, B6;129P2-Cbl<tm1(CBL*Q367P)Homy>

RBRC No. RBRC05655
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Cbl<tm1(CBL*Q367P)Homy>
Former Common name Cbl Q367P cKI, B6-Cbl/CBL*Q367P KI
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2012. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6 mice. Neomycin selection cassette was removed by crossing with FLPe mice.
Strain description Exon 5 to 9 of Cbl (Casitas B-lineage lymphoma) gene was replaced with a loxP flanked human CBL, and a human CBL having Q367P variant was inserted the downstream.
Colony maintenance Heterozygote x Wild-type [C57BL/6NCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol CBL
Symbol name Casitas B-lineage lymphoma(human)
Chromosome 9
Common name No Data
Symbol description No Data
2 Symbol Cbl
Symbol name Casitas B-lineage lymphoma
Chromosome 9
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 9
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 9
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 9
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04609, B6;129P2-Wnt5a<tm1.1Homy>

RBRC No. RBRC04609
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Wnt5a<tm1.1Homy>
Former Common name Wnt5a-floxed
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 14
Common name No Data
Symbol description No Data
2 Symbol Wnt5a
Symbol name wingless-related MMTV integration site 5A
Chromosome 14
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 14
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 14
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
Developmental Biology Research,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
WNT5a is required for normal ovarian follicle development and antagonizes gonadotropin responsiveness in granulosa cells by suppressing canonical WNT signaling. FASEB J.30: 1534-1547 (2016).(26667040)
Abedini A, Zamberlam G, Lapointe E, Tourigny C, Boyer A, Paquet M, Hayashi K, Honda H, Kikuchi A, Price C, Boerboom D.

RBRC05921, B6;129P2-Eed<tm1(L240Q)Homy>

RBRC No. RBRC05921
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Eed<tm1(L240Q)Homy>
Former Common name Eed L240Q KI mice
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2012. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6 mice. Neomycin selection cassette was removed by crossing with Cre mice.
Strain description Eed L240Q mutant knock-in mice. L240Q mutation in the EED gene was found from patients with myelodysplastic syndromes.
Colony maintenance Heterozygote x Wild-type [C57BL/6NCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol Eed
Symbol name embryonic ectoderm development
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Developmental Biology Research,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04841, B6N.129P2-Bhlhe40<tm1Homy>

RBRC No. RBRC04841
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6N.129P2-Bhlhe40<tm1Homy>
Former Common name Dec1 KO
H-2 Haplotype No Data
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Bhlhe40
Symbol name basic helix-loop-helix family, member e40
Chromosome 6
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (Streptomyces fradiase)
Chromosome 6
Common name neo; neomycin;
Symbol description No Data
References

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Icariin protects against glucocorticoid induced osteoporosis, increases the expression of the bone enhancer DEC1 and modulates the PI3K/Akt/GSK3 beta/beta-catenin integrated signaling pathway. Biochem. Pharmacol.136: 109-121 (2017).(28408345)
Hu J, Mao Z, He S, Zhan Y, Ning R, Liu W, Yan B, Yang J.

RBRC05922, B6;129P2-Eed<tm1(I363M)Homy>

RBRC No. RBRC05922
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129P2-Eed<tm1(I363M)Homy>
Former Common name IEed I363M K
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hiroaki Honda, Research Institute for Radiation Biology and Medicine, Hiroshima University in 2012. E14 ES cells (129P2/OlaHsd) were used. The mutant mice were crossed to C57BL/6 mice. Neomycin selection cassette was removed by crossing with Cre mice.
Strain description Eed I363M mutant knock-in mice. I363M mutation in the EED gene was found from patients with myelodysplastic syndromes.
Colony maintenance Heterozygote x Wild-type [C57BL/6NCrlCrlj]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Eed
Symbol name embryonic ectoderm development
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Developmental Biology Research,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Additional information
1 No Data
Depositor Honda, Hiroaki (Tokyo Women’s Medical University) Honda, Hiroaki
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months