RBRC05290, B6.129P2-Dscam<tm1.1Kzy>/A151Kzy

RBRC No. RBRC05290
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Dscam<tm1.1Kzy>/A151Kzy
Former Common name B6-Dscam KO#A151
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Exon 1 was removed by crossing with CAG-cre (B6 background). The mice were backcrossed to C57BL/6 over 21 times.
Strain description Dscam knockout mice. Dscam, Down syndrome cell adhesion molecule is a gene of neural Ig superfamily mediates cell adhesion and axon outgrowth. Homozygous mutant mice of C57BL/6 genetic background die within 24 hours after birth due to central respiratory defects. In BALB/c mixed background, Homozygous mutant mice are viable and survive to adulthood.
Colony maintenance Heterozygote x Wild-type [C57BL/6NCrSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol Dscam
Symbol name Down syndrome cell adhesion molecule
Chromosome 16
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 16
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Mouse Models for Human Disease,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Amano et al., J. Neurosci., 29(9):2984-2996. 2009
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Mouse of the Month Jan 2012
2 Genotyping protocol <PCR>
Depositor Yamakawa, Kazuhiro (RIKEN) Yamakawa, Kazuhiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06358, B6.129P2-Scn1a<tm1.1Kzy>

RBRC No. RBRC06358
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Scn1a<tm1.1Kzy>
Former Common name Scn1a KI, B6-Scn1a KI
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Ikuo Ogiwara and Kazuhiro Yamakawa, RIKEN Brain Science Institute in 2007. E14 ES cells were used to generate the mutant mice. After ackcrossing to C57BL/6J (JAX) for 5 times, a frt flanked sequence were removed by crossing with CAG-FLPe mice (B6 background), and then the mice were backcrossed to C57BL/6J(JAX) over 9 times.
Strain description Scn1a GENE R1407* nonsense mutation knock-in mice. Homozygous mutant mice show ataxia from around 10 days after birth and die up before 25 days-old due to malnutrition. Heterozygous mutant mice show epileptic seizure from around 14 days after birth, and about 30-50% mice die a sudden death at 20 to 40 days-old. Model mice for dravet syndrome.
Colony maintenance Heterozygote x Wild-type [C57BL/6J(Crlj)]
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 2
Common name No Data
Symbol description No Data
2 Symbol Scn1a
Symbol name sodium channel, voltage-gated, type I, alpha
Chromosome 2
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 2
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neurosci., 27, 5903-5914 (2007).
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Mouse of the Month Oct 2014
Depositor Yamakawa, Kazuhiro (RIKEN) Yamakawa, Kazuhiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09420, STOCK Scn1a<tm1.1Kzy>

RBRC No. RBRC09420
Type Targeted MutationCartagena
Species Mus musculus
Strain name STOCK Scn1a<tm1.1Kzy>
Former Common name No Data
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Ikuo Ogiwara and Kazuhiro Yamakawa, RIKEN Brain Science Institute in 2007. E14 ES cells were used to generate the mutant mice. After backcrossing to C57BL/6J (JAX) for 5 times, a frt flanked sequence were removed by crossing with CAG-FLPe mice (B6 background), and then the mice were backcrossed to C57BL/6J(JAX) over 9 times. Then, mice were further backcrossed to B6D2.
Strain description Scn1a GENE R1407* nonsense mutation knock-in mice. Homozygous mutant mice show ataxia from around 10 days after birth and die up before 25 days-old due to malnutrition. Heterozygous mutant mice show epileptic seizure from around 14 days after birth, and about 30-50% mice die a sudden death at 20 to 40 days-old. Model mice for Dravet syndrome. C57BL/6 genetic background (RBRC06358), B6D2 mixed genetic background (RBRC09420).
Health Report
BRC facility Health Report in the last year and a half

Examination Date Room Rack
Jan 15, 2018 2-検 L
Nov 13, 2017 2-検 L
Sep 11, 2017 2-検 L
Jul 10, 2017 2-検 L
May 15, 2017 2-検 L
Mar 13, 2017 2-検 L
Jan 16, 2017 2-検 L
Nov 14, 2016 2-検 L

Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 2
Common name No Data
Symbol description No Data
2 Symbol Scn1a
Symbol name sodium channel, voltage-gated, type I, alpha
Chromosome 2
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 2
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Neurosci., 27, 5903-5914 (2007).
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Yamakawa, Kazuhiro (RIKEN) Yamakawa, Kazuhiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC10241, B6-Tg(Scn1a-GFP)1Kzy

RBRC No. RBRC10241
Type TransgeneCartagena
Species Mus musculus
Strain name B6-Tg(Scn1a-GFP)1Kzy
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 polyA
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Scn1a promoter
2 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Depositor Yamakawa, Kazuhiro (RIKEN) Yamakawa, Kazuhiro
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC10242, B6.129-Scn1a<tm2.1Kzy>

RBRC No. RBRC10242
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129-Scn1a<tm2.1Kzy>
Former Common name No Data
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 2
Common name No Data
Symbol description No Data
2 Symbol Scn1a
Symbol name sodium channel, voltage-gated, type I, alpha
Chromosome 2
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 2
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 2
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Human Molecular Genetics, 22(23), 4784-4804 (2013).
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Yamakawa, Kazuhiro (RIKEN) Yamakawa, Kazuhiro
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC10243, B6.129-Scn2a<tm1Mml>

RBRC No. RBRC10243
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6.129-Scn2a<tm1Mml>
Former Common name No Data
H-2 Haplotype No Data
ES cell line R1 [(129X1/SvJ x 129S1/Sv)F1-Kitl<+>]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Scn2a
Symbol name sodium channel, voltage-gated, type II, alpha
Chromosome 2
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 2
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Biophys. J., 78(6), 2878-2891 (2000).
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEVELOPER (Prof. Maurice Montal, Section of Neurobiology, Division of Biological Sciences, University of California San Diego, USA, E-mail: mmontal@ucsd.edu) using the Approval Form.
2) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEVELOPER (Prof. Maurice Montal) in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Yamakawa, Kazuhiro (RIKEN) Yamakawa, Kazuhiro
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC10468, B6.129-Scn2a<tm2.1Kzy>

RBRC No. RBRC10468
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129-Scn2a<tm2.1Kzy>
Former Common name Scn2a-floxed, Scn2afl/+
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 2
Common name No Data
Symbol description No Data
2 Symbol Scn2a
Symbol name sodium channel, voltage-gated, type II, alpha
Chromosome 2
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 2
Common name No Data
Symbol description No Data
4 Symbol loxP
Symbol name phage P1 loxP
Chromosome 2
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Ogiwara, et al., (2018) Communications Biologyvolume 1, Article number: 96.
https://doi.org/10.1038/s42003-018-0099-2
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Yamakawa, Kazuhiro (RIKEN) Yamakawa, Kazuhiro
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC10469, B6.129-Scn2a<tm1.1Kzy>

RBRC No. RBRC10469
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129-Scn2a<tm1.1Kzy>
Former Common name Scn2a-knock-in, Scn2aRX/+
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 2
Common name No Data
Symbol description No Data
2 Symbol Scn2a
Symbol name sodium channel, voltage-gated, type II, alpha
Chromosome 2
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 2
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
Ogiwara, et al., (2018) Communications Biologyvolume 1, Article number: 96.
https://doi.org/10.1038/s42003-018-0099-2
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Yamakawa, Kazuhiro (RIKEN) Yamakawa, Kazuhiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC02715, B6.129P2-Epm2a<tm1Kzy>

RBRC No. RBRC02715
Type Targeted MutationCartagena wks
Species Mus musculus
Strain name B6.129P2-Epm2a<tm1Kzy>
Former Common name Epm2a-KO
H-2 Haplotype No Data
ES cell line E14 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Epm2a
Symbol name epilepsy, progressive myoclonic epilepsy, type 2 gene alpha
Chromosome 10
Common name laforin
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 10
Common name neo; neomycin;
Symbol description No Data
References

Research applications Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Hum. Mol. Genet., 11, 1251-1262 (2002).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use with the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Yamakawa, Kazuhiro (RIKEN) Yamakawa, Kazuhiro
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months