RBRC03797, 129S6-Egflam<tm1Tfir>

RBRC No. RBRC03797
Type Targeted MutationCartagena
Species Mus musculus
Strain name 129S6-Egflam<tm1Tfir>
Former Common name 129S6/SvEvTac-Egflam
H-2 Haplotype No Data
Background strain 129S6/SvEvTac
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Egflam
Symbol name EGF-like, fibronectin type III and laminin G domains
Chromosome 15
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome 15
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 15
Common name No Data
Symbol description No Data
References Nat Neurosci. 2008 Aug;11(8):923-31. Epub 2008 Jul 20.

Pikachurin, a dystroglycan ligand, is essential for photoreceptor ribbon synapse formation.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Neurosci., 11, 923-931 (2008).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Furukawa, Takahisa (Osaka Bioscience Institute) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06220, B6.Cg-Tg(Syn1-creERT2)1Tfur

RBRC No. RBRC06220
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(Syn1-creERT2)1Tfur
Former Common name Syn1-CreERT2 Tg, B6-Syn1-CreER Tg
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Yoshihiro Omori and Takahisa Furukawa, Institute for Protein Research, Osaka University in 2012. The transgene was injected into the pronuclei of B6C3F1 fertilized eggs. The mutant mice were crossed to C57BL/6.
Strain description These transgenic mice express the cre ERT2 under the control of the rat 4.3 kb synapsin 1 promoter.
Colony maintenance Carrier x Noncarrier [C57BL/6]
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 poly A
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol ERT2
Symbol name estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter rat Synapsin1 promoter
3 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Furukawa, Takahisa (Institute for Protein Research, Osaka University) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC09434, B6CD1-Tg(Pax2-cre)1Akg

RBRC No. RBRC09434
Type TransgeneCartagena
Species Mus musculus
Strain name B6CD1-Tg(Pax2-cre)1Akg
Former Common name Pax2-Cre mice
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Ires
Symbol name internal ribosomal entry site (EMCV)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol poly A
Symbol name SV40 polyadenylation site
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genesis, 38(4):195-199 (2004).
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr. Andy Grove (@ Baylor College of Medicine) and the DEPOSITOR using the Approval Form.
Additional information
1 No Data
Depositor Furukawa, Takahisa (Institute for Protein Research, Osaka University) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC10305, B6;B6D2-Lrit2<em1Tfur>

RBRC No. RBRC10305
Type CRISPR/Cas9
Species Mus musculus
Strain name B6;B6D2-Lrit2<em1Tfur>
Former Common name Lrit2 KO
H-2 Haplotype No Data
Background strain C57BL/6JJmsSlc
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Lrit2
Symbol name leucine-rich repeat, immunoglobulin-like and transmembrane domains 2
Chromosome 14
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Cell Reports, 22, 3548-3561 (2018)
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE is requested to contact the depositor (Dr. Takahisa Furukawa) and enter into a Material Transfer Agreement with Osaka University.
Additional information
1 No Data
Depositor Furukawa, Takahisa (Institute for Protein Research, Osaka University) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05138, 129S6-Trpm1<tm1Tfur>

RBRC No. RBRC05138
Type Targeted MutationCartagena
Species Mus musculus
Strain name 129S6-Trpm1<tm1Tfur>
Former Common name 129-Trpm1 KO
H-2 Haplotype No Data
Background strain 129S6/SvEvTac
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takahisa Furukawa, Osaka Bioscience Institute in 2005. A neomycin selection cassette was inserted into the exon 4-6 of the TRPM1 gene. 129 background.
Strain description Trpm1 knockout mice. A neomycin selection cassette was inserted into the exon 4-6 of the TRPM1 gene.
Colony maintenance Homozygote x Homozygote
Health Report
Gene Details
Promoter No Data
1 Symbol Trpm1
Symbol name transient receptor potential cation channel, subfamily M, member 1
Chromosome 7
Common name No Data
Symbol description No Data
References

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Proc. Natl. Acad. Sci. USA,, 107, 332-337(2010).
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
Additional information
1 Mouse of the Month Sep 2011
2 Genotyping protocol <PCR>
Depositor Furukawa, Takahisa (Osaka Bioscience Institute) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06356, B6(C3)-Lgalsl<tm1(KOMP)Vlcg>

RBRC No. RBRC06356
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6(C3)-Lgalsl<tm1(KOMP)Vlcg>
Former Common name LgalsL KO, Lgalsl KO
H-2 Haplotype No Data
ES cell line VGB6 [C57BL/6NTac]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Yoshihiro Omori and Takahisa Furukawa, Institute for Protein Research, Osaka University. The original allele were from the Knockout Mouse Project (KOMP) Repository.
Strain description Lgalsl gene knockout mice. Homozygous mutant mice are viable and fertile.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 polyA
Chromosome 11
Common name No Data
Symbol description No Data
2 Symbol Lgalsl
Symbol name lectin, galactoside binding-like
Chromosome 11
Common name No Data
Symbol description No Data
3 Symbol Pgk1
Symbol name mouse PGK polyA signal
Chromosome 11
Common name No Data
Symbol description No Data
4 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 11
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data
Promoter human ubiquitin C promoter
6 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University). The use of the BIOLOGICAL RESOURCE is strictly limited to academic, non-profit research institutes for academic non-profit research purposes. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested.
Additional information
1 No Data
Depositor Furukawa, Takahisa (Institute for Protein Research, Osaka University) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09474, C57BL/6-Tmem119<tm1Tfur>

RBRC No. RBRC09474
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6-Tmem119<tm1Tfur>
Former Common name B6-Obif-KO, B6-Obif<tm1Tfur>
H-2 Haplotype No Data
ES cell line JM8A3 [C57BL/6N-A]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takahisa Furukawa, Osaka Bioscience Institute in 2015.
Strain description Obif, osteoblast induction factor gene knockout mice. Exon 2 of Obif gene was replaced with PGK-neo cassette.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Tmem119
Symbol name transmembrane protein 119
Chromosome 5
Common name obif
Symbol description No Data
2 Symbol No Data
Symbol name SV40 Poly A signal
Chromosome 5
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 5
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
Additional information
1 No Data
Depositor Furukawa, Takahisa (Institute for Protein Research, Osaka University) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC10662, B6;BDF1-Lrtm1<em1Tfur>

RBRC No. RBRC10662
Type CRISPR/Cas9
Species Mus musculus
Strain name B6;BDF1-Lrtm1<em1Tfur>
Former Common name Lrtm1 KO
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Lrtm1
Symbol name leucine-rich repeats and transmembrane domains 1
Chromosome 14
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
RECIPIENT which wants to use the BIOLOGICAL RESOURCE is requested to contact the depositor (Dr. Takahisa Furukawa) and enter into a Material Transfer Agreement with Osaka University.
Additional information
1 No Data
Depositor Furukawa, Takahisa (Institute for Protein Research, Osaka University) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC05426, 129.B6C3-Tg(Crx-cre)1Tfur

RBRC No. RBRC05426
Type TransgeneCartagena
Species Mus musculus
Strain name 129.B6C3-Tg(Crx-cre)1Tfur
Former Common name 129-Crx-cre Tg
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takahisa Furukawa, Osaka Bioscience Institute in 2003. The transgene was injected into the B6C3F1 fertilized eggs. The transgenic mice were backcrossed to 126S6 mice over 6 generations.
Strain description This transgenic mouse expresses Cre recombinase gene in retinal photoreceptor cells and progenitor cells under the control of the 12-kb mouse Crx promoter.
Colony maintenance Carrier x Noncarrier [or Crossing to 129S6/SvEvTac]
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Crx promoter
2 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Sensorineural Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nat. Neurosci., 6, 1255-1263 (2003).
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Furukawa, Takahisa (Osaka Bioscience Institute) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC06569, B6(C3)-Ift80<tm1c(KOMP)Wtsi>

RBRC No. RBRC06569
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6(C3)-Ift80<tm1c(KOMP)Wtsi>
Former Common name Ift80 flox, B6-Ift80 floxed
H-2 Haplotype No Data
ES cell line JM8A1.N3 [C57BL/6N-A]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Yoshihiro Omori and Takahisa Furukawa, Institute for Protein Research, Osaka University. The original allele were from the Knockout Mouse Project (KOMP) Repository. JM8A1.N3 ES cells were used to generate the mutant mice. Floxed mice (tm1c allele mice) were generated by crossing with CAG-FLPe mice.
Strain description Ift80 gene floxed mice. Exon 6 was flanked by loxP sites.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6NCrSlc]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 3
Common name No Data
Symbol description No Data
2 Symbol Ift80
Symbol name intraflagellar transport 80
Chromosome 3
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 3
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must contract an MTA with the DEPOSITOR (Osaka University). The use of the BIOLOGICAL RESOURCE is strictly limited to academic, non-profit research institutes for academic non-profit research purposes. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR and a mention of the Knockout Mouse Project and the KOMP Repository as the source of the original allele and mice are requested.
Additional information
1 No Data
Depositor Furukawa, Takahisa (Institute for Protein Research, Osaka University) Furukawa, Takahisa
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months