RBRC03096, B6.Cg-Tg(CAG-EGFP/MIR143)A

RBRC No. RBRC03096
Type Transgene CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Tg(CAG-EGFP/MIR143)A
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol MIR143
Symbol name miR-143
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
2 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome No Data
Common name No Data
Symbol description No Data
References Biochem Biophys Res Commun. 2008 Aug 8;372(4):856-61. Epub 2008 Jun 3.
J Biol Chem 2004 Dec 10;279(50):52361-5. Epub 2004 Oct 25.
Mol Cancer Res. 2003 Oct;1(12):882-91.

Establishment of cells to monitor Microprocessor through fusion genes of microRNA and GFP.
Establishment of cells to monitor Microprocessor through fusion genes of microRNA and GFP.
Establishment of cells to monitor Microprocessor through fusion genes of microRNA and GFP.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. All data got with the BIOLOGICAL RESOURCE must be published as a joint result with the DEPOSITER.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Iwamoto, Takashi (Chubu University) Iwamoto, Takashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC03098, B6.Cg-Tg(CAG-EGFP/MIR145/MIR143)A

RBRC No. RBRC03098
Type Transgene CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Tg(CAG-EGFP/MIR145/MIR143)A
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol MIR143
Symbol name miR-143
Chromosome No Data
Common name No Data
Symbol description No Data
2 Symbol MIR145
Symbol name miR-145
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
3 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome No Data
Common name No Data
Symbol description No Data
References Biochem Biophys Res Commun. 2008 Aug 8;372(4):856-61. Epub 2008 Jun 3.
J Biol Chem 2004 Dec 10;279(50):52361-5. Epub 2004 Oct 25.
Mol Cancer Res. 2003 Oct;1(12):882-91.

Establishment of cells to monitor Microprocessor through fusion genes of microRNA and GFP.
Establishment of cells to monitor Microprocessor through fusion genes of microRNA and GFP.
Establishment of cells to monitor Microprocessor through fusion genes of microRNA and GFP.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. All data got with the BIOLOGICAL RESOURCE must be published as a joint result with the DEPOSITER.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Iwamoto, Takashi (Chubu University) Iwamoto, Takashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC03099, B6.Cg-Tg(CAG-EGFP/MIR145/MIR143)B

RBRC No. RBRC03099
Type Transgene CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Tg(CAG-EGFP/MIR145/MIR143)B
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol MIR143
Symbol name miR-143
Chromosome No Data
Common name No Data
Symbol description No Data
2 Symbol MIR145
Symbol name miR-145
Chromosome No Data
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
3 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome No Data
Common name No Data
Symbol description No Data
References Biochem Biophys Res Commun. 2008 Aug 8;372(4):856-61. Epub 2008 Jun 3.
J Biol Chem 2004 Dec 10;279(50):52361-5. Epub 2004 Oct 25.
Mol Cancer Res. 2003 Oct;1(12):882-91.

Establishment of cells to monitor Microprocessor through fusion genes of microRNA and GFP.
Establishment of cells to monitor Microprocessor through fusion genes of microRNA and GFP.
Establishment of cells to monitor Microprocessor through fusion genes of microRNA and GFP.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. All data got with the BIOLOGICAL RESOURCE must be published as a joint result with the DEPOSITER.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Iwamoto, Takashi (Chubu University) Iwamoto, Takashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC03712, B6;129P2-Dgcr8<Gt(XG058)Byg>

RBRC No. RBRC03712
Type Gene Trap
Species Mus musculus
Strain name B6;129P2-Dgcr8<Gt(XG058)Byg>
Former Common name DGCR8KO/XG058
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description DGCR8 is a key molecule of biogenesis of microRNA. DGCR8-/- mice are embryonic lethal and DGCR8-/+ mice look healthy but could be models of human 22q11.2 deletion syndrome including schizophrenia.
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name No Data
Chromosome No Data
Common name No Data
Symbol description No Data
References Nat Genet. 2007 Jan 28;.
Nat Genet. 2008 Jun;40(6):751-60. Epub 2008 May 11.

DGCR8 is essential for microRNA biogenesis and silencing of embryonic stem cell self-renewal.
DGCR8 is essential for microRNA biogenesis and silencing of embryonic stem cell self-renewal.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of research papers. The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEPOSITOR/DEVELOPER. All data got with this BIOLOGICAL RESOURCE must be published as a joint result with the DEPOSITOR.
Additional information
1 Lab HP (Japanese)
2 Genotyping protocol <PCR>
Depositor Iwamoto, Takashi (Chubu University) Iwamoto, Takashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04323, B6;B6C-Tg(CAG-EGFP/miR21)

RBRC No. RBRC04323
Type TransgeneCartagena
Species Mus musculus
Strain name B6;B6C-Tg(CAG-EGFP/miR21)
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description miR-21 is a potent tumor promoting gene and may be involved in cardiac hypertrophy. Their expression can be monitored by GFP.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name No Data
Chromosome No Data
Common name No Data
Symbol description No Data
References Nature. 2008 Dec 18;456(7224):980-4. Epub 2008 Nov 30.

MicroRNA-21 contributes to myocardial disease by stimulating MAP kinase signalling in fibroblasts.
MicroRNA-21 contributes to myocardial disease by stimulating MAP kinase signalling in fibroblasts.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
後日指定
For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Lab HP (Japanese)
Depositor Iwamoto, Takashi (Chubu University) Iwamoto, Takashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05652, B6.Cg-Tg(alpha-MHC/miR143/145)9

RBRC No. RBRC05652
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(alpha-MHC/miR143/145)9
Former Common name B6-aMHC/miR143/145 Tg#9
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takashi Iwamoto, College of Life and Health Sciences, Chubu University in 2010. The transgene was injected into the (C57BL/6 x BALB/c)F2 fertilized eggs. The mice were crossed to C57BL/6J over 5 generations.
Strain description Transgenic mice expressing miR-143 and miR-145 in cardiomyocytes. Their hearts generally show low %FS after 2 months old and become enlarged.
Colony maintenance Carrier x Noncarrier [C57BL/6J(Crlj)]
Tg mice show a high incidence of sudden death after 2-3 months old.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol miR145
Symbol name pri-miR-145 (human)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter rat alpha-myosine heavy chain promoter
2 Symbol miR143
Symbol name pri-miR-143 (human)
Chromosome UN
Common name No Data
Symbol description No Data
References Biochem Biophys Res Commun. 2008 Aug 8;372(4):856-61. Epub 2008 Jun 3.

Establishment of cells to monitor Microprocessor through fusion genes of microRNA and GFP.
Establishment of cells to monitor Microprocessor through fusion genes of microRNA and GFP.
Research applications Cardiovascular Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (will be announced). Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval form the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Iwamoto, Takashi (Chubu University) Iwamoto, Takashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05653, B6.Cg-Tg(alpha-MHC/miR143/145)19

RBRC No. RBRC05653
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(alpha-MHC/miR143/145)19
Former Common name B6-aMHC/miR143/145 Tg#19
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takashi Iwamoto, College of Life and Health Sciences, Chubu University in 2010. The transgene was injected into the (C57BL/6 x BALB/c)F2 fertilized eggs. The mice were crossed to C57BL/6J over 5 generations.
Strain description Transgenic mice expressing miR-143 and miR-145 in cardiomyocytes. Their hearts generally show low %FS after 2 months old and become enlarged.
Colony maintenance Carrier x Noncarrier [C57BL/6J(Crlj)]
Tg mice show a high incidence of sudden death after 2-3 months old.
Health Report
Gene Details
Promoter No Data
1 Symbol miR145
Symbol name pri-miR-145 (human)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter rat alpha-myosine heavy chain promoter
2 Symbol miR143
Symbol name pri-miR-143 (human)
Chromosome UN
Common name No Data
Symbol description No Data
References Biochem Biophys Res Commun. 2008 Aug 8;372(4):856-61. Epub 2008 Jun 3.

Establishment of cells to monitor Microprocessor through fusion genes of microRNA and GFP.
Establishment of cells to monitor Microprocessor through fusion genes of microRNA and GFP.
Research applications Cardiovascular Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (will be announced). Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval form the DEPOSITOR using the Approval Form. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Iwamoto, Takashi (Chubu University) Iwamoto, Takashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09429, C57BL-Tg(alpha-MHC/miR145)287

RBRC No. RBRC09429
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL-Tg(alpha-MHC/miR145)287
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description These strains overexpress miR-145 in cardiomyocytes.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol GH
Symbol name Growh hormone polyA (human)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Alpha-myosine heavy chain promoter
2 Symbol MIR145
Symbol name microRNA 145
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
後日指定
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Additional information
1 No Data
Depositor Iwamoto, Takashi (Chubu University) Iwamoto, Takashi
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09430, C57BL-Tg(alpha-MHC/miR145)417

RBRC No. RBRC09430
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL-Tg(alpha-MHC/miR145)417
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol GH
Symbol name Growh hormone polyA (human)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter Rat Alpha-myosine heavy chain promoter
2 Symbol MIR143
Symbol name microRNA 143
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
後日指定
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Iwamoto, Takashi (Chubu University) Iwamoto, Takashi
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09558, C57BL-Tg(villin-Lin28A)A

RBRC No. RBRC09558
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL-Tg(villin-Lin28A)A
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse villin promoter
2 Symbol Lin28A
Symbol name lin-28 homolog A
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
(後日指定)
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The RECIPIENT agrees to use the BIOLOGICAL RESOURSE only for publication of research papers.The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Iwamoto, Takashi (Chubu University) Iwamoto, Takashi
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months