RBRC02412, B6.Cg-Tg(Nes-cre)1Nogu

RBRC No. RBRC02412
Type Transgene CongenicCartagena wks
Species Mus musculus
Strain name B6.Cg-Tg(Nes-cre)1Nogu
Former Common name B6.Cg-Tg(Nes-cre)1Nogu
H-2 Haplotype No Data
Background strain C57BL/6JJcl
1 Appearance No Data
Genotype No Data
Strain development Developed by Dr. Shigeru Noguchi, Meiji Dairies Corporation and Kanagawa Academy of Science and Technology and Dr. Toshikuni Sasaoka, Center for Bioresource-based Researches, Brain Research Institute, Niigata University. The transgene was injected into the pronuclei of B6D2F1 fertilized eggs. The mice were backcrossed to C57BL/6.
Strain description Nestin-cre transgenic mice, expressing a cre recombinase gene under the control of Nestin promoter and enhancer. This Nestin-cre transgene contains a rat nestin promoter, cre recombinase gene, IRES-lacZ-polyA and neuron specific enhancer (around 100 bp of exon 1, the entire intron 1 and around 100 bp of exon 3 of nesitin gene). Tissue-specific expression: observed in whole brain, spinal cord and testis/ovary; not observed in thymus, lung, heart, kidney, spleen, stomach, small and large intestine, pancreas, skeletal muscle and tail (Developers, personal communication).
Colony maintenance Backcross to C57BL/6 (Hemizygote x C57BL/6JJcl)
Health Report
Gene Details
Promoter No Data
1 Symbol Nes
Symbol name nestin
Chromosome UN
Common name ESTM46
Symbol description No Data
2 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome UN
Common name internal ribosomal entry site from EMCV
Symbol description No Data
3 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
4 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome UN
Common name No Data
Symbol description E. coli beta galactosidase
5 Symbol poly A
Symbol name SV40 poly A signal
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature(s) designated by the DEPOSITOR is requested. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for publication of academic research. Prior to publication, the RECIPIENT should contact the developer scientist, Dr. Shigeru Noguchi and acknowledge Drs. Shigeru Noguchi and Dr. Toshikuni Sasaoka.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Nishimura, Koichiro (Kanagawa Institute of Industrial Science and Technology) Nishimura, Koichiro
Strain Status /
(Expected delivery)

Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live mouse :
BRC mice in Publications
Title Journal
Prostaglandin e2-mediated attenuation of mesocortical dopaminergic pathway is critical for susceptibility to repeated social defeat stress in mice. J Neurosci.32(12): 4319-29 (2012).(22442093)
Tanaka K, Furuyashiki T, Kitaoka S, Senzai Y, Imoto Y, Segi-Nishida E, Deguchi Y, Breyer RM, Breyer MD, Narumiya S.
Overview and assessment of the histochemical methods and reagents for the detection of -galactosidase activity in transgenic animals. Anat Sci Int. (2015).(26394634)
Trifonov S, Yamashita Y, Kase M, Maruyama M, Sugimoto T.