RBRC02237, C57BL/6Cr-Tg(Dmp1-HBEGF)

RBRC No. RBRC02237
Type TransgeneCartagena wks
Species Mus musculus
Strain name C57BL/6Cr-Tg(Dmp1-HBEGF)
Former Common name DTR-9.6 kb Tg
H-2 Haplotype No Data
Background strain C57BL/6NCrSlc
Appearance
1 Appearance black
Genotype a/a B/B C/C
Strain development Developed by Drs. Tatsumi and Ikeda, National Center for Geriatrics and Gerontology in 2003.
Strain description Bone remodeling consists of two distinct stages, resorption and formation, which are performed by osteoclasts and osteoblasts, respectively. Dmp1-HBEGF transgenic mice express human HB-EGF as the receptor for diphtheria toxin (DT) on the cell surface of osteocytes under the control of Dmp1 promoter. Most of the osteocytes (70 to 80%) can be ablated in these transgenic mice by injection of DT. The osteocytes-ablated mice develop osteoporosis, even in young age, showing fragile bone with intracortical porosity and microfractures, osteoblastic dysfunction, and loss of trabecular bone with microstructural deterioration and adipose tissue proliferation in the marrow space. Interestingly, the mice do not respond to mechanical unloading with bone loss. This strain is a useful model for the study of osteoporosis and mechanotransduction in bone.
Colony maintenance Backcross to C57BL/6 (Hemizygote x C57BL/6CrSlc)
Health Report
Gene Details
Promoter Dmp1 (dentin matrix protein 1) promoter
1 Symbol HBEGF
Symbol name heparin-binding EGF-like growth factor (human)
Chromosome UN
Common name No Data
Symbol description No Data
References Cell Metab. 2007 Jun;5(6):464-75.

Targeted ablation of osteocytes induces osteoporosis with defective mechanotransduction.
Research applications Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Cell Metab., 6, 464-475, (2007).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 Mouse of the Month Oct 2007
2 Genotyping protocol <PCR>
Depositor Ikeda, Kyoji (National Center for Geriatrics and Gerontology) Ikeda, Kyoji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Skeletal adaptation to intramedullary pressure-induced interstitial fluid flow is enhanced in mice subjected to targeted osteocyte ablation. PLoS One.7(3): e33336 (2012).(22413015)
Kwon RY, Meays DR, Meilan AS, Jones J, Miramontes R, Kardos N, Yeh JC, Frangos JA.
The Role of Osteocytes in Bone Resorption during Orthodontic Tooth Movement. J Dent Res. (2013).(23358677)
Matsumoto T, Iimura T, Ogura K, Moriyama K, Yamaguchi A.

RBRC06142, B6.129-Tnfsf11<tm1>

RBRC No. RBRC06142
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129-Tnfsf11<tm1>
Former Common name RANKL flox, B6-Tnfsf11 floxed
H-2 Haplotype No Data
ES cell line Not specified (ES cell)
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Toshio Fumoto and Kyoji Ikeda, National Center for Geriatrics and Gerontology (2009-2013). 129Sv/EvTac derived ES cells were used. Frt flanked neo cassette was removed by crossing with CAG-FLPe (B6). The mutant mice were crossed C57BL/6 OVER 9 times.
Strain description RANKL (Tnfsf11) gene floxed mice. Exons 3 and 4 were flanked by loxP site.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJmsSlc] Homozygous mice show no obvious abnormality.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 14
Common name No Data
Symbol description No Data
2 Symbol Tnfsf11
Symbol name tumor necrosis factor (ligand) superfamily, member 11
Chromosome 14
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 14
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system,
Mouse Models for Human Disease
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Bone. Miner. Res., 29, 830-842 (2014).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Ikeda, Kyoji (National Center for Geriatrics and Gerontology) Ikeda, Kyoji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06522, B6.Cg-Cthrc1<tm1.1Suna>

RBRC No. RBRC06522
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Cthrc1<tm1.1Suna>
Former Common name Cthrc1 flox, B6-Cthrc1 floxed
H-2 Haplotype No Data
ES cell line iTL BA1[(C57BL/6N x 129S6/SvEvTac)F1]
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Sunao Takeshita, National Center for Geriatrics and Gerontology. (C57BL/6NTac x 129S6/SvEvTac)F1 derived ES cells were used to generate the mutant mice. C57BL/6 background. Floxed mice (RBRC06522): A frt-flanked neo cassette was removed by crossing with CAG-FLPe mice. KO mice (RBRC06523): A frt-flanked neo cassette was removed by crossing with CAG-FLPe mice, and then the exon 2 was removed by crossing with CAG-Cre mice.
Strain description Cthrc1 gene floxed mice (RBRC06522)(Exon 2 was flanked by loxP sites). Cthrc1 gene KO mice (RBRC06523)(Exon 2 was deleted).
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Cthrc1
Symbol name collagen triple helix repeat containing 1
Chromosome 15
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 15
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 15
Common name No Data
Symbol description No Data
References

Research applications Cancer Research,
Cre/loxP system,
FLP/frt system,
Hematological Research,
Metabolism Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Clin. Invest., 123, 3914-3924 (2013).
Additional information
1 No Data
Depositor Ikeda, Kyoji (National Center for Geriatrics and Gerontology) Ikeda, Kyoji
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06523, B6.Cg-Cthrc1<tm1.2Suna>

RBRC No. RBRC06523
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Cthrc1<tm1.2Suna>
Former Common name Cthrc1 sKO, B6-Cthrc1 KO
H-2 Haplotype No Data
ES cell line iTL BA1[(C57BL/6N x 129S6/SvEvTac)F1]
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Sunao Takeshita, National Center for Geriatrics and Gerontology. (C57BL/6NTac x 129S6/SvEvTac)F1 derived ES cells were used to generate the mutant mice. C57BL/6 background. Floxed mice (RBRC06522): A frt-flanked neo cassette was removed by crossing with CAG-FLPe mice. KO mice (RBRC06523): A frt-flanked neo cassette was removed by crossing with CAG-FLPe mice, and then the exon 2 was removed by crossing with CAG-Cre mice.
Strain description Cthrc1 gene floxed mice (RBRC06522)(Exon 2 was flanked by loxP sites). Cthrc1 gene KO mice (RBRC06523)(Exon 2 was deleted).
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Cthrc1
Symbol name collagen triple helix repeat containing 1
Chromosome 15
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 15
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 15
Common name No Data
Symbol description No Data
References

Research applications Cancer Research,
Cre/loxP system,
FLP/frt system,
Hematological Research,
Metabolism Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Clin. Invest., 123, 3914-3924 (2013).
Additional information
1 No Data
Depositor Ikeda, Kyoji (National Center for Geriatrics and Gerontology) Ikeda, Kyoji
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06524, C57BL/6-Tg(CAG-Cthrc1)1Suna

RBRC No. RBRC06524
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(CAG-Cthrc1)1Suna
Former Common name Cthrc1 Tg#1, B6-CAG-Cthrc1 Tg#1
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Sunao Takeshita, National Center for Geriatrics and Gerontology. The transgene was injected into the pronuclei of C57BL/6N fertilized eggs.
Strain description CAG-Cthrc1 transgenic mice. Line 1 (RBRC06524)(1 copy). Line 100 (main line)(RBRC06525)(100 copies).
Colony maintenance Carrier x Noncarrier [C57BL/6N]
Health Report No Data
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
1 Symbol Cthrc1
Symbol name collagen triple helix repeat containing 1
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Cancer Research,
Hematological Research,
Metabolism Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Clin. Invest., 123, 3914-3924 (2013).
Additional information
1 No Data
Depositor Ikeda, Kyoji (National Center for Geriatrics and Gerontology) Ikeda, Kyoji
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06525, C57BL/6-Tg(CAG-Cthrc1)100Suna

RBRC No. RBRC06525
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(CAG-Cthrc1)100Suna
Former Common name Cthrc1 Tg#100, B6-CAG-Cthrc1 Tg#100
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Sunao Takeshita, National Center for Geriatrics and Gerontology. The transgene was injected into the pronuclei of C57BL/6N fertilized eggs.
Strain description CAG-Cthrc1 transgenic mice. Line 1 (RBRC06524)(1 copy). Line 100 (main line)(RBRC06525)(100 copies).
Colony maintenance Carrier x Noncarrier [C57BL/6N]
Health Report No Data
Gene Details
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
1 Symbol Cthrc1
Symbol name collagen triple helix repeat containing 1
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Cancer Research,
Hematological Research,
Metabolism Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
J. Clin. Invest., 123, 3914-3924 (2013).
Additional information
1 No Data
Depositor Ikeda, Kyoji (National Center for Geriatrics and Gerontology) Ikeda, Kyoji
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC02230, B6.Cg-Tg(CAG-Ggt1)

RBRC No. RBRC02230
Type Transgene CongenicCartagena wks
Species Mus musculus
Strain name B6.Cg-Tg(CAG-Ggt1)
Former Common name GGT Tg, C57BL/6Cr
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol HBB2
Symbol name rabbit beta-globin poly A
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
3 Symbol Ggt1
Symbol name gamma-glutamyltransferase 1
Chromosome UN
Common name CD224, GGT, Ggtp
Symbol description No Data
References Endocrinology. 2007 Mar 15;.
J Biol Chem 2004 Feb 13;279(7):5752-6. Epub 2003 Nov 21.

Overexpression of {gamma}-glutamyltransferase in transgenic mice accelerates bone resorption and causes osteoporosis.
Overexpression of {gamma}-glutamyltransferase in transgenic mice accelerates bone resorption and causes osteoporosis.
Research applications Metabolism Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Endocrinology, 148(6):2708-2715 (2007).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional information
1 No Data
Depositor Ikeda, Kyoji (National Center for Geriatrics and Gerontology) Ikeda, Kyoji
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months