RBRC04201, C57BL/6-Tg(Tie2-Cxcl12)1Tng

RBRC No. RBRC04201
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(Tie2-Cxcl12)1Tng
Former Common name Tie2-CXCL12
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter mouse Tie2 promotor/enhancer
1 Symbol Cxcl12
Symbol name chemokine (C-X-C motif) ligand 12
Chromosome UN
Common name No Data
Symbol description No Data
References Immunity. 2003 Aug;19(2):257-67.

Long-term hematopoietic stem cells require stromal cell-derived factor-1 for colonizing bone marrow during ontogeny.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT is requested to make a Material Transfer Agreement with the DEPOSITOR. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent form the DEPOSITOR.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Nagasawa, Takashi (Institute for frontier medical sciences, Kyoto university) Nagasawa, Takashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04197, B6.129P2-Cxcr4<tm1Tng>

RBRC No. RBRC04197
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Cxcr4<tm1Tng>
Former Common name CXCR4 KO
H-2 Haplotype No Data
ES cell line E14.1 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Cxcr4
Symbol name chemokine (C-X-C motif) receptor 4
Chromosome 1
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 1
Common name No Data
Symbol description No Data
References

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT is requested to make a Material Transfer Agreement with the DEPOSITOR. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent form the DEPOSITOR.
Additional information
1 No Data
Depositor Nagasawa, Takashi (Institute for frontier medical sciences, Kyoto university) Nagasawa, Takashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04198, B6.129P2-Cxcr4<tm2Tng>

RBRC No. RBRC04198
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Cxcr4<tm2Tng>
Former Common name CXCR4 flox
H-2 Haplotype No Data
ES cell line E14.1 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Cxcr4
Symbol name chemokine (C-X-C motif) receptor 4
Chromosome 1
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 1
Common name No Data
Symbol description No Data
References Immunity. 2004 Jun;20(6):707-18.

Cellular niches controlling B lymphocyte behavior within bone marrow during development.
Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT is requested to make a Material Transfer Agreement with the DEPOSITOR. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent form the DEPOSITOR.
Additional information
1 Mouse of the Month Dec 2014
2 Genotyping protocol <PCR>
Depositor Nagasawa, Takashi (Institute for frontier medical sciences, Kyoto university) Nagasawa, Takashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04199, B6.129P2-Cxcl12<tm1Tng>

RBRC No. RBRC04199
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Cxcl12<tm1Tng>
Former Common name CXCL12 KO
H-2 Haplotype No Data
ES cell line E14.1 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Cxcl12
Symbol name chemokine (C-X-C motif) ligand 12
Chromosome 6
Common name No Data
Symbol description No Data
Promoter herpes simplex virus thymidine kinase promoter (HSV tk promoter)
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name neo; neomycin;
Symbol description No Data
References

Research applications Immunology and Inflammation Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT is requested to make a Material Transfer Agreement with the DEPOSITOR. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent form the DEPOSITOR.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Nagasawa, Takashi (Institute for frontier medical sciences, Kyoto university) Nagasawa, Takashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
CXCL12 in early mesenchymal progenitors is required for haematopoietic stem-cell maintenance. Nature.495(7440): 227-30 (2013).(23434756)
Greenbaum A, Hsu YM, Day RB, Schuettpelz LG, Christopher MJ, Borgerding JN, Nagasawa T, Link DC.

RBRC04200, B6.129P2-Cxcl12<tm2Tng>

RBRC No. RBRC04200
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129P2-Cxcl12<tm2Tng>
Former Common name CXCL12-GFP
H-2 Haplotype No Data
ES cell line E14.1 [129P2/OlaHsd]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Takashi Nagasawa, Research Institute, Osaka Medical Center for Maternal and Child Health. E14.1 ES cells were used. The mutant mice were backcrossed to C57BL/6J for 6 times, and then maintained by sibling mating.
Strain description CXCL12-GFP knock-in mice. Exon 1 of the Cxcl12 gene was replaced with a GFP expression cassette.
Colony maintenance Heterozygote x Wild-type [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Cxcl12
Symbol name chemokine (C-X-C motif) ligand 12
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name neo; neomycin;
Symbol description No Data
References Immunity. 2003 Aug;19(2):257-67.

Long-term hematopoietic stem cells require stromal cell-derived factor-1 for colonizing bone marrow during ontogeny.
Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT is requested to make a Material Transfer Agreement with the DEPOSITOR. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent form the DEPOSITOR.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Mouse of the Month Dec 2014
3 Genotyping protocol <PCR>
Depositor Nagasawa, Takashi (Institute for frontier medical sciences, Kyoto university) Nagasawa, Takashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months