RBRC00041, C;B6-a/a·b/b-W Sh/+

RBRC No. RBRC00041
Type Spontaneous Mutation 8wks,♂
Species Mus musculus
Strain name C;B6-a/a·b/b-WSh/+
Former Common name C;B6-a/a·b/b-WSh/+, Sash brown, B6C3-WSh/+ (brown)
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance brown
Genotype No Data
Strain development C57BL/6-WSh/+ was gifted from Dr. Yukihiko Kitamura, Osaka University to Dr. Kusakabe M, RIKEN Tsukuba Institute.
Strain description Homozygotes are viable, males are sterile.
Health Report
Gene Details
Promoter No Data
1 Symbol Kit
Symbol name kit oncogene
Chromosome 5
Common name No Data
Symbol description No Data
References Am J Pathol. 2005 Sep;167(3):835-48.
Blood. 1992 Sep 15;80(6):1448-53.
Blood. 1994 Jun 15;83(12):3509-16.
Blood. 1999 Oct 15;94(8):2658-66.
Development. 1993 Jul;118(3):705-17.

Mast cell-deficient W-sash c-kit mutant Kit W-sh/W-sh mice as a model for investigating mast cell biology in vivo.
Mast cell-deficient W-sash c-kit mutant Kit W-sh/W-sh mice as a model for investigating mast cell biology in vivo.
Mast cell-deficient W-sash c-kit mutant Kit W-sh/W-sh mice as a model for investigating mast cell biology in vivo.
Mast cell-deficient W-sash c-kit mutant Kit W-sh/W-sh mice as a model for investigating mast cell biology in vivo.
Mast cell-deficient W-sash c-kit mutant Kit W-sh/W-sh mice as a model for investigating mast cell biology in vivo.
Research applications No Data
Specific Term and Conditions No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor RIKEN, BioResource Center
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC00323, B6C3-mip2

RBRC No. RBRC00323
Type Spontaneous Mutation
Species Mus musculus
Strain name B6C3-mip2
Former Common name B6C3-Ph-eyeless, Patch-eyeless, B6C3-Ph (small eye)
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Cxcl2
Symbol name patch deletion region
Chromosome 5
Common name patch
Symbol description No Data
References No Data

Research applications Sensorineural Research
Specific Term and Conditions No specific terms and conditions. (The DEPOSITOR waives its own rights under any patents, intellectual property, or other proprietary rights with respect to the results to be obtained by use of the BIOLOGICAL RESOURCE.)
Additional information
1 No Data
Depositor RIKEN, BioResource Center
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC03801, C57BL/6N-Tg(Vil1-cre)2Utr/Rbrc

RBRC No. RBRC03801
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6N-Tg(Vil1-cre)2Utr/Rbrc
Former Common name Villin-NLSCre
H-2 Haplotype No Data
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Laboratory Animal Resource Center, Tsukuba University and RIKEN BioResource Center in 2009. C57BL/6N background.
Strain description Cre-driver transgenic mice. A transgenic construct containing sequence encoding Cre recombinase under the control of the mouse villin 1 promoter fragment (intestinal stem cells specific promoter region) derived from the BAC library was microinjected into C57BL/6NCrlCrlj fertilized eggs. Tissue-specific expression of Cre recombinase has not been examined yet. Users are encouraged to test the tissue-specificity and feed back the data for the research community.
Colony maintenance Carrier x Noncarrier [C57BL/6NCrlCrlj]. Hemizygous Tg mice are viable and fertile (viability and fertility of homozygotes are unknown).
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Villin gene promoter (intestinal stem cells specific promoter region)
4 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Ken-ichi Yagami, Laboratory Animal Resource Center, University of Tsukuba and RIKEN BioResource Center is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 Mouse of the Month Jul 2013
2 Genotyping protocol <PCR>
Depositor RIKEN, BioResource Center
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC04206, C57BL/6N-Tg(CAG-KO,Halo/GFP)477-7Sasa/Rbrc

RBRC No. RBRC04206
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6N-Tg(CAG-KO,Halo/GFP)477-7Sasa/Rbrc
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6NCrlCrlj
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by National Institute for Basic Biology and RIKEN BioResource Center in 2010. C57BL/6N background.
Strain description Optogenetic transgenic mice. A transgenic construct containing CAG promoter, a loxP-KO (Kusabira Orange)-STOP-loxP sequence and a Halo (halorhodopsin)-GFP fusion cDNA was microinjected into C57BL/6NCrlCrlj fertilized eggs. Expression of KO and Halo/GFP has not been examined yet. Users are encouraged to test the expression pattern and feed back the data for the research community.
Colony maintenance Carrier x Noncarrier [C57BL/6NCrlCrlj]. Hemizygous Tg mice are viable and fertile (viability and fertility of homozygotes are unknown).
Health Report
BRC facility Health Report in the last year and a half

Examination Date Room Rack
Sep 3, 2012 2-検 A
Jul 2, 2012 2-検 A
May 7, 2012 2-検 A
Mar 5, 2012 2-検 A
Jan 10, 2012 2-検 A
Nov 7, 2011 2-検 A
Sep 5, 2011 2-検 A
Jul 4, 2011 2-検 A

Gene Details
Promoter No Data
1 Symbol GFP
Symbol name Green Fluorescent Protein (Jellyfish)
Chromosome No Data
Common name No Data
Symbol description No Data
2 Symbol KO
Symbol name Kusabira Orange
Chromosome No Data
Common name No Data
Symbol description No Data
References No Data

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Toshikuni Sasaoka, National Institute for Basic Biology and RIKEN BioResource Center is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Depositor RIKEN, BioResource Center
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05463, C57BL/6N-Trpc3<tm1a(EUCOMM)Wtsi>/D08

RBRC No. RBRC05463
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6N-Trpc3<tm1a(EUCOMM)Wtsi>/D08
Former Common name B6-Trpc3#D08
H-2 Haplotype No Data
ES cell line JM8A3.N1 [C57BL/6N-A]
Background strain C57BL/6NTac
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Trpc3
Symbol name transient receptor potential cation channel, subfamily C, member 3
Chromosome 3
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions For inquiries please mail us at animal@brc.riken.jp.
Additional information
1 No Data
Depositor RIKEN, BioResource Center
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05681, C57BL/6N-Penk<tm1(KOMP)Mbp>/G01

RBRC No. RBRC05681
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6N-Penk<tm1(KOMP)Mbp>/G01
Former Common name No Data
H-2 Haplotype No Data
ES cell line JM8A3.N1 [C57BL/6N-A]
Background strain C57BL/6NTac
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol En2
Symbol name En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)
Chromosome 4
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 4
Common name No Data
Symbol description No Data
3 Symbol Ires
Symbol name internal ribosomal entry site (EMCV)
Chromosome 4
Common name No Data
Symbol description No Data
4 Symbol Penk
Symbol name preproenkephalin
Chromosome 4
Common name No Data
Symbol description No Data
5 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 4
Common name No Data
Symbol description No Data
6 Symbol poly A
Symbol name SV40 polyadenylation site
Chromosome 4
Common name No Data
Symbol description No Data
Promoter human beta-actin promoter
7 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 4
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT should acknowledge the KOMP, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor RIKEN, BioResource Center
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06261, C57BL/6N-Clspn<tm1(KOMP)Vlcg>/AA8

RBRC No. RBRC06261
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6N-Clspn<tm1(KOMP)Vlcg>/AA8
Former Common name No Data
H-2 Haplotype No Data
ES cell line VGB6 [C57BL/6NTac]
Background strain C57BL/6NTac
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Clspn
Symbol name claspin
Chromosome 4
Common name No Data
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT should acknowledge the KOMP, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively.
Additional information
1 No Data
Depositor RIKEN, BioResource Center
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06348, C57BL/6N-Cck<tm2b(EUCOMM)Hmgu>/B06

RBRC No. RBRC06348
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6N-Cck<tm2b(EUCOMM)Hmgu>/B06
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6NTac
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Cck
Symbol name cholecystokinin
Chromosome 9
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT should acknowledge the EUCOMM, RIKEN BRC and the International Mouse Phenotyping Consortium as the original creator of the targeted mouse ES cells and the provider of the knockout mice, respectively.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor RIKEN, BioResource Center
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06499, C57BL/6N-Gucy2c<em2Rbrc>

RBRC No. RBRC06499
Type CRISPR/Cas9
Species Mus musculus
Strain name C57BL/6N-Gucy2c<em2Rbrc>
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6NTac
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Gucy2c
Symbol name guanylate cyclase 2c
Chromosome 6
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT should acknowledge RIKEN BRC and the International Mouse Phenotyping Consortium for creating and providing of the knockout mice.
Additional information
1 No Data
Depositor RIKEN, BioResource Center
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09300, C57BL/6N-Katna1<em1Rbrc>

RBRC No. RBRC09300
Type CRISPR/Cas9
Species Mus musculus
Strain name C57BL/6N-Katna1<em1Rbrc>
Former Common name No Data
H-2 Haplotype No Data
Background strain C57BL/6NTac
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Katna1
Symbol name katanin p60 (ATPase-containing) subunit A1
Chromosome 10
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
The RECIPIENT should acknowledge RIKEN BRC and the International Mouse Phenotyping Consortium for creating and providing of the knockout mice.
Additional information
1 No Data
Depositor RIKEN, BioResource Center
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months