RBRC06322, B6.Cg-Edn1<tm1Ywa>

RBRC No. RBRC06322
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Edn1<tm1Ywa>
Former Common name ET-1 flox (ET1 flox), B6-Edn1 floxed
H-2 Haplotype No Data
ES cell line SM1[129S6/SvEvTac]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Masashi Yanagisawa, The University of Texas Southwestern Medical Center. SM1 ES cells were used to generate the mutant mice.
Strain description ET1 (Edn1 / endothelin 1) gene floxed mice. Exon 2 was flanked by loxP sites.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6J(Crlj)]
Health Report
Gene Details
Promoter No Data
1 Symbol Edn1
Symbol name endothelin 1
Chromosome 13
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 13
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Developmental Biology Research,
Hematological Research,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Proc. Natl. Acad. Sci. U S A., 101, 2088-2093 (2004).
Depositor limits the use of the BIOLOGICAL RESOURCE to not-for-profit institutions. Depositor limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipients who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the Depositor. Recipient shall acquire prior written consent from the Depositor prior to filing an application for patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Yanagisawa, Masashi (University of Texas Southwestern Medical Center at Dallas) Yanagisawa, Masashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06323, B6.Cg-Ednra<tm2Ywa>

RBRC No. RBRC06323
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Ednra<tm2Ywa>
Former Common name ETA flox, B6-Ednra floxed
H-2 Haplotype No Data
ES cell line Not specified (ES cell)
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Masashi Yanagisawa, The University of Texas Southwestern Medical Center.
Strain description ETA (Ednra / endothelin receptor type A) gene floxed mice. Exon 6 to 7 were flanked by loxP sites.
Colony maintenance Homozygote x Homoztgote [or Crossing to C57BL/6J(Crlj)]
Health Report
Gene Details
Promoter No Data
1 Symbol Ednra
Symbol name endothelin receptor type A
Chromosome 8
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 8
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Hematological Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Mo.l Cell Biol., 23, 8226-8232 (2003).
Depositor limits the use of the BIOLOGICAL RESOURCE to not-for-profit institutions. Depositor limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipients who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the Depositor. Recipient shall acquire prior written consent from the Depositor prior to filing an application for patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Yanagisawa, Masashi (University of Texas Southwestern Medical Center at Dallas) Yanagisawa, Masashi
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06324, B6.Cg-Tg(DBH-Ednrb)Ywa

RBRC No. RBRC06324
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(DBH-Ednrb)Ywa
Former Common name ETB KO/DBH-ETB Tg, B6-DBH-Ednrb Tg
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Masashi Yanagisawa, The University of Texas Southwestern Medical Center. 129 and C57BL/6 mixed background.
Strain description DBH-ETB transgenic mice. These mice express a Ednrb gene under the control of the human DBH promoter.
Colony maintenance Carrier x Carrier [or Crossing to C57BL/6J(Crlj)]
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name mouse protamine genomic DNA
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name rat insulin genomic DNA
Chromosome UN
Common name No Data
Symbol description No Data
Promoter human Dopamine b-hydroxylase promoter
3 Symbol Ednrb
Symbol name endothelin receptor type B (Rat)
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Developmental Biology Research,
Hematological Research,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Circulation., 106, 1991-1998 (2002).
Depositor limits the use of the BIOLOGICAL RESOURCE to not-for-profit institutions. Depositor limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipients who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the Depositor. Recipient shall acquire prior written consent from the Depositor prior to filing an application for patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Yanagisawa, Masashi (University of Texas Southwestern Medical Center at Dallas) Yanagisawa, Masashi
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06327, B6;129-Ffar3<tm1Reh>

RBRC No. RBRC06327
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129-Ffar3<tm1Reh>
Former Common name Gpr41 KO, B6-Ffar3 KO
H-2 Haplotype No Data
ES cell line SM1[129S6/SvEvTac]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Masashi Yanagisawa, The University of Texas Southwestern Medical Center. SM1 ES cells derived from 129S6/SvEvTac mice were used to generate the mutant mice. The mice were crossed to C57BL/6.
Strain description The Gpr41 (Ffar3) exon coding region was replaced with a nLacZ and floxed neo cassette.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6J(Crjl)]
Health Report
Gene Details
Promoter No Data
1 Symbol Ffar3
Symbol name free fatty acid receptor 3
Chromosome 7
Common name Gpr41
Symbol description No Data
2 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 7
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
4 Symbol nls
Symbol name SV40 large T antigen nuclear localization signal (NLS)
Chromosome 7
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 7
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Proc. Natl. Acad. Sci. U S A. 105, 16767-1672 (2008).
Depositor limits the use of the BIOLOGICAL RESOURCE to not-for-profit institutions. Depositor limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipients who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the Depositor. Recipient shall acquire prior written consent from the Depositor prior to filing an application for patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Yanagisawa, Masashi (University of Texas Southwestern Medical Center at Dallas) Yanagisawa, Masashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09472, B6.129-Nms<tm1.1Ywa>Nmu<tm1Mko>

RBRC No. RBRC09472
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.129-Nms<tm1.1Ywa>Nmu<tm1Mko>
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 1
Common name No Data
Symbol description No Data
2 Symbol Nms
Symbol name neuromedin S
Chromosome 1
Common name No Data
Symbol description No Data
3 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome 1
Common name No Data
Symbol description No Data
4 Symbol Nmu
Symbol name neuromedin U
Chromosome 5
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
5 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 5
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron. 2015 Mar 4;85(5):1086-102. doi: 10.1016. DEPOSITOR limits the use of BIOLOGICAL RESOURCE to not-for-profit institutions. DEPOSITOR limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipient who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the DEPOSITOR. Recipient shall acquire prior written consent from the DEPOSITOR prior to filing an application for patent or intellectual property or their rights based on the results of research using the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Yanagisawa, Masashi (University of Texas Southwestern Medical Center at Dallas) Yanagisawa, Masashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09473, C57BL/6-Tg(Nms-IRES-iCre)

RBRC No. RBRC09473
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(Nms-IRES-iCre)
Former Common name No Data
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol iCre
Symbol name phage P1 codon improved Cre (iCre)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Nms promoter
2 Symbol Ires
Symbol name internal ribosomal entry site (EMCV)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron. 2015 Mar 4;85(5):1086-102. doi: 10.1016. DEPOSITOR limits the use of BIOLOGICAL RESOURCE to not-for-profit institutions. DEPOSITOR limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipient who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the DEPOSITOR. Recipient shall acquire prior written consent from the DEPOSITOR prior to filing an application for patent or intellectual property or their rights based on the results of research using the BIOLOGICAL RESOURCE.
Additional information
1 No Data
Depositor Yanagisawa, Masashi (University of Texas Southwestern Medical Center at Dallas) Yanagisawa, Masashi
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09909, C57BL/6N-Nalcn<em1Iiis>

RBRC No. RBRC09909
Type CRISPR/Cas9
Species Mus musculus
Strain name C57BL/6N-Nalcn<em1Iiis>
Former Common name Nalcn
H-2 Haplotype No Data
Background strain C57BL/6NJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development The EEG/EMG-based dominant screening of ENU-mutagenized mice yielded a mutant pedigree with a REMS abnormality, which we termed Dreamless (Drl). The founder of the pedigree showed a short REMS episode duration. Whole-exome sequencing combined with direct sequencing of candidate genes identified a heterozygous missense mutation in the Nalcn gene (chr14: 123515403). We then confirmed the causal relationship of the Nalcn gene mutation to the REMS phenotype by introducing the same nucleotide substitution in wild-type mice using the CRISPR/Cas9 system. CRISPR NalcnDrl/+ mice showed a short REMS episode duration, similar to the original Dreamless pedigree.
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Nalcn
Symbol name sodium leak channel, non-selective
Chromosome 14
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature, 539, 378-383 (2016).
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr. Masashi Yanagisawa. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Additional information
1 No Data
Depositor Yanagisawa, Masashi (University of Tsukuba) Yanagisawa, Masashi
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09910, C57BL/6N-Sik3<tm1.1Iiis>

RBRC No. RBRC09910
Type Targeted MutationCartagena
Species Mus musculus
Strain name C57BL/6N-Sik3<tm1.1Iiis>
Former Common name Sik3
H-2 Haplotype No Data
ES cell line RENKA [C57BL/6NCrlCrlj]
Background strain C57BL/6NJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development We induced random point mutations by ethylnitrosourea (ENU) and screened for dominant sleep and wakefulness abnormalities through EEG/EMG-based sleep staging. Through our screening, we established a mutant pedigree, which we termed Sleepy (Slp), with a markedly prolonged sleep time. Whole-exome sequencing of Sleepy mutants identified a heterozygous single nucleotide substitution at the splice donor site for intron 13 of the Sik3 gene. To confirm genetically that the Sik3 splice mutation is the sole cause of the long-sleep phenotype, we introduced the Sik3 exon 13-skipping allele into wild-type mice by using conventional knock-in in embryonic stem (ES) cells. As expected, these mouse lines exhibited markedly reduced wake time, similar to the original Sleepy pedigree.
Strain description This mouse line exhibited markedly reduced wake time.
Health Report
Gene Details
Promoter No Data
1 Symbol Sik3
Symbol name SIK family kinase 3
Chromosome 9
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome No Data
Common name No Data
Symbol description No Data
References No Data

Research applications FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature, 539, 378-383 (2016).
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr. Masashi Yanagisawa. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Yanagisawa, Masashi (University of Tsukuba) Yanagisawa, Masashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04495, B6.Cg-Tg(Tek-cre)1Ywa

RBRC No. RBRC04495
Type TransgeneCartagena
Species Mus musculus
Strain name B6.Cg-Tg(Tek-cre)1Ywa
Former Common name Tie2-Cre, B6-TekCre Tg
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Masashi Yanagisawa, The University of Texas Southwestern Medical Center. Mixed background.
Strain description Tek-cre (Tie2-cre) transgenic mice. These mice express a cre recombinase gene under the control of the mouse Tek promoter.
Colony maintenance Carrier x Noncarrier [or Crossing to C57BL/6N(Crlj)]
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name mouse metallothionein-1(MT-1) poly A signal
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse endothelial-specific receptor tyrosine kinase promoter
2 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Dev. Biol., 230, 230-242 (2001).
Depositor limits the use of the BIOLOGICAL RESOURCE to not-for-profit institutions. Depositor limits the use of the BIOLOGICAL RESOURCE to academic purposes. For-profit institutions and recipients who intend to use BIOLOGICAL RESOURCE for commercial research purposes shall acquire prior written consent from the Depositor. Recipient shall acquire prior written consent from the Depositor prior to filing an application for patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor Yanagisawa, Masashi (University of Texas Southwestern Medical Center at Dallas) Yanagisawa, Masashi
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :
BRC mice in Publications
Title Journal
(PMID)
Author
O-GlcNAc on NOTCH1 EGF repeats regulates ligand-induced Notch signaling and vascular development in mammals. Elife6: (2017).(28395734)
Sawaguchi S, Varshney S, Ogawa M, Sakaidani Y, Yagi H, Takeshita K, Murohara T, Kato K, Sundaram S, Stanley P, Okajima T.

RBRC09911, C57BL/6N-Sik3<em2Iiis>

RBRC No. RBRC09911
Type CRISPR/Cas9 (Transgene)Cartagena
Species Mus musculus
Strain name C57BL/6N-Sik3<em2Iiis>
Former Common name Sik3
H-2 Haplotype No Data
Background strain C57BL/6NJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development We induced random point mutations by ethylnitrosourea (ENU) and screened for dominant sleep and wakefulness abnormalities. Through our screening, we established a mutant pedigree, which we termed Sleepy (Slp), with a markedly prolonged sleep time. Whole-exome sequencing identified a heterozygous single nucleotide substitution at the splice donor site for intron 13 of the Sik3 gene. Further examine this function, we created the mice in which a Flag-tag was inserted into the N terminus of SIK3 (wildtype) using CRISPR/Cas9 technology.
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol FLAG
Symbol name FLAG tag (synthetic)
Chromosome 9
Common name No Data
Symbol description No Data
2 Symbol HA
Symbol name human influenza virus HA (hemagglutinin) tag sequence
Chromosome 9
Common name No Data
Symbol description No Data
3 Symbol Sik3
Symbol name SIK family kinase 3
Chromosome 9
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Nature, 539, 378-383 (2016).
Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from Dr. Masashi Yanagisawa. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research.
Additional information
1 No Data
Depositor Yanagisawa, Masashi (University of Tsukuba) Yanagisawa, Masashi
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months