RBRC02275, B6.Cg-Fam101b<tm1Sia>/SiaRbrc

RBRC No. RBRC02275
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Fam101b<tm1Sia>/SiaRbrc
Former Common name cfm(-) (Acc. No. CDB0025K)
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Fam101b
Symbol name family with sequence similarity 101, member B
Chromosome 11
Common name cfm
Symbol description No Data
2 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 11
Common name neo; neomycin;
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 11
Common name No Data
Symbol description No Data
4 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 11
Common name No Data
Symbol description No Data
5 Symbol HBB2
Symbol name rabbit beta-globin polyA
Chromosome 11
Common name No Data
Symbol description No Data
6 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 11
Common name No Data
Symbol description E. coli beta galactosidase
7 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data
8 Symbol loxP
Symbol name phage P1 loxP
Chromosome 11
Common name No Data
Symbol description No Data
References Gene Expr Patterns. 2005 Feb;5(3):439-44.

cfm is a novel gene uniquely expressed in developing forebrain and midbrain, but its null mutant exhibits no obvious phenotype.
Research applications Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
(1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. CDB0025K and the BIOLOGICAL RESOURCE is requested (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html). (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested, Gene Expression Patterns, 5 439-444 (2005). (3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested, “We acknowledge Dr. AIZAWA and Laboratory for animal Resources and Genetic Engineering in CDB for providing us the cfm mutant mice(Acc. No. CDB0025K).” (4) Please inform the DEVELOPER (mutant.bdr@riken.jp) about the paper when published.
Additional information
1 No Data
Depositor RIKEN, CDB (RIKEN Center for Developmental Biology) RIKEN, CDB
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02794, B6.Cg-Vps52<tm1Kab>/95Rbrc

RBRC No. RBRC02794
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Vps52<tm1Kab>/95Rbrc
Former Common name Vps52 #95(Acc.No.CDB0767K)
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Michihiko Sugimoto and Kuniya Abe, RIKEN BioResource center in 2008. TT2 (B6CBF1 derived) ES cells were used to generate the targeted mice. C57BL/6 and CBA mixed (C57BL/6 semicongenic).
Strain description Vps52 2lox2frt (neo) targeted mice in which Frt-neo cassette (PGK-neo) was inserted upstream of exon 2, and two loxP sites were located in introns 1 and 2. Homozygous mutant mice were embryonic lethal. Line 95 (main line) (RBRC02794), Line 16 (sub line) (RBRC02832), Line 20 (sub line) (RBRC02857).
Colony maintenance Heterozygote x Wild-type [C57BL/6NCrlCrlj]
Health Report
Gene Details
Promoter No Data
1 Symbol Vps52
Symbol name VPS52 GARP complex subunit
Chromosome 17
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 17
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 17
Common name No Data
Symbol description No Data
4 Symbol HBB2
Symbol name rabbit beta-globin
Chromosome 17
Common name No Data
Symbol description No Data
5 Symbol lox71
Symbol name mutant lox sequence
Chromosome 17
Common name No Data
Symbol description No Data
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 17
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
7 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 17
Common name neo; neomycin;
Symbol description No Data
References

Research applications Cre/loxP system,
Developmental Biology Research,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
(1) The RECIPIENT shall obtain a prior agreement from Dr. Kuniya Abe (abe@brc.riken.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0767K and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested (Cell Rep. 2012 2(5):1363-74.). (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
Additional information
1 Lab HP
2 Genotyping protocol <PCR>
Depositor RIKEN, CDB (RIKEN Center for Developmental Biology) RIKEN, CDB
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC03512, B6.Cg-Rspo2<tm1Hoka>/Rbrc

RBRC No. RBRC03512
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Rspo2<tm1Hoka>/Rbrc
Former Common name R-spondin2(lacZ) (Acc.No. CDB0467K)
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 15
Common name No Data
Symbol description No Data
2 Symbol HBB2
Symbol name rabbit beta-globin polyA
Chromosome 15
Common name No Data
Symbol description No Data
3 Symbol LacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 15
Common name No Data
Symbol description No Data
4 Symbol Rspo2
Symbol name R-spondin 2 homolog (Xenopus laevis)
Chromosome 15
Common name No Data
Symbol description No Data
5 Symbol lox71
Symbol name mutant lox sequence
Chromosome 15
Common name No Data
Symbol description No Data
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 15
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
7 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 15
Common name No Data
Symbol description No Data
References Dev Growth Differ. 2008 Feb;50(2):85-95. Epub 2007 Dec 7.

R-spondin2 expression in the apical ectodermal ridge is essential for outgrowth and patterning in mouse limb development.
Research applications Cre/loxP system,
Developmental Biology Research,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
(1) The RECIPIENT shall obtain a prior agreement from Dr. Hitoshi Okamoto (hitoshi@brain.riken.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0467K) (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Dev Growth Differ. 2008 50(2):85-95.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor RIKEN, CDB (RIKEN Center for Developmental Biology) RIKEN, CDB
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC04402, aquaporin 4 (GFP)-6 (Acc.No. CDB0758K-1)

RBRC No. RBRC04402
Type Targeted MutationCartagena
Species Mus musculus
Strain name aquaporin 4 (GFP)-6 (Acc.No. CDB0758K-1)
Former Common name No Data
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Masato Yasui, School of Medicine, Keio University/Johns Hopkins University and Laboratory for Animal Resources and Genetic Engineering, RIKEN Center for Developmental Biology.
Strain description aquaporin 4 gene GFP knock-in mice. EGFP-Pgk-neo type (#5 ES clone derived)(B6 mixed: RBRC04397, BALB/c background: RBRC04399), delta Pgk-neo type (#5 ES clone derived)(B6 background: RBRC04364, BALB/c mixed: RBRC04400), delta EGFP-Pgk-neo type (#5 ES clone derived)(B6 mixed: RBRC04401, BALB/c mixed: RBRC04402), EGFP-Pgk-neo type (#50 ES clone derived)(B6 mixed: RBRC04398), delta Pgk-neo type (#50 ES clone derived)(B6 mixed: RBRC04403).
Colony maintenance Homozygote x Homozygote [or Crossing to BALB/cAJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name No Data
Chromosome No Data
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
(1) The RECIPIENT shall obtain a prior agreement from Dr. Masato Yasui (myasui@a3.keio.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0758K-1) (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (未発表) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor RIKEN, CDB (RIKEN Center for Developmental Biology) RIKEN, CDB
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05277, B6.Cg-Fgfbp3<tm1Tohe>/Rbrc

RBRC No. RBRC05277
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Fgfbp3<tm1Tohe>/Rbrc
Former Common name Fgf-bp3 (Acc. No.CDB0444K)
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Tatsutoshi Nakahata, Graduate School of Medicine, Kyoto University.
Strain description Fgfbp3, fibroblast growth factor binding protein 3 knockout mice. Homozygous mutant mice show increased anxiety-related responses.
Colony maintenance Heterozygote x Wild-type [C57BL/6N]
Health Report
Gene Details
Promoter No Data
1 Symbol Fgfbp3
Symbol name fibroblast growth factor binding protein 3
Chromosome 19
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 19
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 19
Common name No Data
Symbol description No Data
4 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 19
Common name No Data
Symbol description No Data
5 Symbol lox71
Symbol name mutant lox sequence
Chromosome 19
Common name No Data
Symbol description No Data
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 19
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
7 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 19
Common name neo; neomycin;
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER (Dr. Tatsutoshi Nakahata, Kyoto University, E-mail: tnakaha@cira.kyoto-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0444K and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (Mol Cell Neurosci. 2011 46(1):200-12.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
Additional information
1 No Data
Depositor RIKEN, CDB (RIKEN Center for Developmental Biology) RIKEN, CDB
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06302, NEAT1(lacZ)(Acc.No.CDB0773K)

RBRC No. RBRC06302
Type Targeted MutationCartagena
Species Mus musculus
Strain name NEAT1(lacZ)(Acc.No.CDB0773K)
Former Common name NEAT1 KO, B6-Neat1 KO, NEAT1(lacZ)(Acc.No.CDB0773K)
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Shinichi Nakagawa, RIKEN Advanced Science Institute. TT2 ES cells were used to generate the knockout mice. A FRT-flanked neo cassette was removed by crossing with FLP mice. The mutant mice were backcrossed to C57BL/6N.
Strain description Neat1 ncRNA knock-out mice. A lacZ was inserted immediately downstream of the transcription start site of Neat1.
Colony maintenance Heterozygote x Wild-type [C57BL/6NCrSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 19
Common name No Data
Symbol description No Data
2 Symbol Neat1
Symbol name nuclear paraspeckle assembly transcript 1 (non-protein coding)
Chromosome 19
Common name No Data
Symbol description No Data
3 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 19
Common name No Data
Symbol description No Data
4 Symbol lox71
Symbol name mutant lox sequence
Chromosome 19
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 19
Common name No Data
Symbol description No Data
References

Research applications Cell Biology Research,
Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
(1) The RECIPIENT shall obtain a prior agreement from Dr. Shinichi Nakagawa (nakagawas@riken.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No.CDB0773K and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested (will be announced). (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor RIKEN, CDB (RIKEN Center for Developmental Biology) RIKEN, CDB
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09343, MOR215-1(swap)(Acc.No.CDB0545K)

RBRC No. RBRC09343
Type Targeted MutationCartagena
Species Mus musculus
Strain name MOR215-1(swap)(Acc.No.CDB0545K)
Former Common name No Data
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name No Data
Chromosome No Data
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
(1) The RECIPIENT shall obtain a prior agreement from the DEVELOPER Dr. Hitoshi Sakano (The University of Tokyo)(satano@u-fukui.acjp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0545K and Web Site (www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested (Cell. 2013 154(6):1314-25.). (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
Additional information
1 No Data
Depositor RIKEN, CDB (RIKEN Center for Developmental Biology) RIKEN, CDB
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02276, B6.Cg-Zic5<tm1Sia>/SiaRbrc

RBRC No. RBRC02276
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Zic5<tm1Sia>/SiaRbrc
Former Common name Opr(-) (Acc. No. CDB0027K)
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description No Data
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Zic5
Symbol name zinc finger protein of the cerebellum 5
Chromosome 14
Common name 1700049L20Rik, odd-paired related, Opr
Symbol description No Data
2 Symbol IRES
Symbol name internal ribosomal entry site (EMCV)
Chromosome 14
Common name internal ribosomal entry site from EMCV
Symbol description No Data
3 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 14
Common name neo; neomycin;
Symbol description No Data
4 Symbol HBB2
Symbol name rabbit beta-globin polyA
Chromosome 14
Common name No Data
Symbol description No Data
5 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 14
Common name No Data
Symbol description E. coli beta galactosidase
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 14
Common name No Data
Symbol description No Data
7 Symbol loxP
Symbol name phage P1 loxP
Chromosome 14
Common name No Data
Symbol description No Data
References Dev Dyn. 2005 Apr;232(4):1056-61.

Characterization of Opr deficiency in mouse brain: subtle defects in dorsomedial telencephalon and medioventral forebrain.
Research applications Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
(1) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. CDB0027K and the BIOLOGICAL RESOURCE is requested (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html). (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested, Developmental Dynamics 232, 1056-1061 (2005). (3) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested, “We acknowledge Dr. AIZAWA for providing us the Opr mutant mice(Acc. No. CDB0027K).” (4) Please inform the DEVELOPER (mutant.bdr@riken.jp) about the paper when published.
Additional information
1 No Data
Depositor RIKEN, CDB (RIKEN Center for Developmental Biology) RIKEN, CDB
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC02832, B6.Cg-Vps52<tm1Kab>/16Rbrc

RBRC No. RBRC02832
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Vps52<tm1Kab>/16Rbrc
Former Common name Vps52 #16(Acc.No.CDB0767K)
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Michihiko Sugimoto and Kuniya Abe, RIKEN BioResource center in 2008. TT2 (B6CBF1 derived) ES cells were used to generate the targeted mice. C57BL/6 and CBA mixed (C57BL/6 semicongenic).
Strain description Vps52 2lox2frt (neo) targeted mice in which Frt-neo cassette (PGK-neo) was inserted upstream of exon 2, and two loxP sites were located in introns 1 and 2. Homozygous mutant mice were embryonic lethal. Line 95 (main line) (RBRC02794), Line 16 (sub line) (RBRC02832), Line 20 (sub line) (RBRC02857).
Colony maintenance Heterozygote x Wild-type [C57BL/6NCrlCrlj]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Vps52
Symbol name VPS52 GARP complex subunit
Chromosome 17
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 17
Common name No Data
Symbol description No Data
3 Symbol HBB2
Symbol name rabbit beta-globin
Chromosome 17
Common name No Data
Symbol description No Data
4 Symbol lox71
Symbol name mutant lox sequence
Chromosome 17
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 17
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
6 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 17
Common name neo; neomycin;
Symbol description No Data
References

Research applications Cre/loxP system,
Developmental Biology Research,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
(1) The RECIPIENT shall obtain a prior agreement from Dr. Kuniya Abe (abe@brc.riken.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Acc.No. CDB0767K and Web. Site (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested (Cell Rep. 2012 2(5):1363-74.). (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
Additional information
1 Lab HP
Depositor RIKEN, CDB (RIKEN Center for Developmental Biology) RIKEN, CDB
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC03513, B6.Cg-Isx<tm1Sse>/Rbrc

RBRC No. RBRC03513
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6.Cg-Isx<tm1Sse>/Rbrc
Former Common name Isx(lacZ) (Acc.No. CDB0475K)
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Susumu Seino, Kobe University Graduate School of Medicine. TT2 ES cells were used to generate the mutant mice.
Strain description Isx gene knockout mice. Exon1 was replaced with a lacZ-neo cassette.
Colony maintenance Heterozygote x Wild-type [C57BL/6NJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 8
Common name No Data
Symbol description No Data
2 Symbol HBB2
Symbol name rabbit beta-globin polyA
Chromosome 8
Common name No Data
Symbol description No Data
3 Symbol Isx
Symbol name intestine specific homeobox
Chromosome 8
Common name No Data
Symbol description No Data
4 Symbol lacZ
Symbol name beta-galactosidase (E. coli)
Chromosome 8
Common name No Data
Symbol description No Data
5 Symbol lox71
Symbol name mutant lox sequence
Chromosome 8
Common name No Data
Symbol description No Data
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 8
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
7 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 8
Common name No Data
Symbol description No Data
References J Biol Chem. 2008 Feb 22;283(8):4905-11. Epub 2007 Dec 19.

Isx participates in the maintenance of vitamin A metabolism by regulation of beta-carotene 15,15′-monooxygenase (Bcmo1) expression.
Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
(1) The RECIPIENT shall obtain a prior agreement from Dr. Susumu Seino (seino@med.kobe-u.ac.jp) to transfer the BIOLOGICAL RESOURCE. (2) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Accession No. (CDB0475K) (http://www2.clst.riken.jp/arg/mutant%20mice%20list.html) and the BIOLOGICAL RESOURCE is requested. (3) In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested. (J Biol Chem. 2008 283(8):4905-11.) (4) Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor RIKEN, CDB (RIKEN Center for Developmental Biology) RIKEN, CDB
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months