RBRC05146, B6;129S6-Gt(ROSA)26Sor<tm1(CAG-mTFP1)Imayo>

RBRC No. RBRC05146
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129S6-Gt(ROSA)26Sor<tm1(CAG-mTFP1)Imayo>
Former Common name R26-CAG-LF-mTFP1
H-2 Haplotype No Data
ES cell line TC1 [129S6/SvEvTac]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description HAtag-attached mTFP1 fluorescent protein is expressed after both Cre- and FLP-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, HA-mTFP1 is expressed under CAG promoter and WPRE sequence is inserted into 3'UTR region.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name STOP cassette (SV40pA-TKpA-SV40pA)
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol GH
Symbol name growth hormone polyadenylation signal
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol HA
Symbol name human influenza virus HA (hemagglutinin) tag sequence
Chromosome 6
Common name No Data
Symbol description No Data
7 Symbol SA
Symbol name SA (splice acceptor sequence)
Chromosome 6
Common name No Data
Symbol description No Data
8 Symbol WPRE
Symbol name Woodchuck hepatitis virus posy-transcriptional regulatory element
Chromosome 6
Common name No Data
Symbol description No Data
9 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
10 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
11 Symbol mTFP1
Symbol name monomeric Teal Fluorescent Protein 1 (Clavularia cFP484)
Chromosome 6
Common name No Data
Symbol description No Data
12 Symbol poly A
Symbol name SV40 late polyA signal
Chromosome 6
Common name No Data
Symbol description No Data
13 Symbol puro
Symbol name puromycin resistance gene (Streptomyces alboniger)
Chromosome 6
Common name No Data
Symbol description No Data
14 Symbol tk
Symbol name HSV thymidine kinase polyA signal
Chromosome 6
Common name No Data
Symbol description No Data
References

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neurosci. Res., 73, 85-91 (2012).
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
Additional information
1 Itaru Imayoshi HP
2 Mouse of the Month Feb 2013
3 Genotyping protocol <PCR>
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05162, B6;129S6-Gt(ROSA)26Sor<tm1(CAG-GCaMP3)Imayo>

RBRC No. RBRC05162
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129S6-Gt(ROSA)26Sor<tm1(CAG-GCaMP3)Imayo>
Former Common name R26-CAG-LF-GCaMP3
H-2 Haplotype No Data
ES cell line TC1 [129S6/SvEvTac]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description GCaMP3 is expressed after both Cre- and FLP-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, GCaMP3 is expressed under CAG promoter and WPRE sequence is inserted into 3'UTR region.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name HSV thymidine kinase polyA signal
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol No Data
Symbol name SV40 late polyA signal
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol GH
Symbol name growth hormone polyadenylation signal
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol WPRE
Symbol name Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)
Chromosome 6
Common name No Data
Symbol description No Data
7 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
8 Symbol GCaMP3
Symbol name RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA
Chromosome 6
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Cell Rep., 9(5):1896-1907 (2014).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR.
Additional information
1 Dr. Loren Looger, MTA
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Itaru Imayoshi HP
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06001, C57BL/6-Tg(Nes-cre/ERT2)6Imayo

RBRC No. RBRC06001
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(Nes-cre/ERT2)6Imayo
Former Common name Nestin-CreERT2 Line6 ; B6-Nes-CreER#6
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2005. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs.
Strain description Nestin-CreER transgenic mice. CreERT2 is expressed by neural stem/progenitor cells. CreERT2-expression level is intermediate.
Colony maintenance Carrier x Noncarrier [C57BL/6JJcl]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol ERT2
Symbol name estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol polyA
Symbol name SV40 PolyA signal
Chromosome UN
Common name No Data
Symbol description No Data
Promoter rat Nestin promoter, Nestin 2nd intron enhancer
3 Symbol cre
Symbol name Phage P1 Cre recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References Genesis. 2006 May;44(5):233-8.

Temporal regulation of Cre recombinase activity in neural stem cells.
Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Genesis. 2006 44(5):233-8.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
Additional information
1 No Data
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC06022, ICR-Tg(Olig2-luc2/Olig2)1Imayo

RBRC No. RBRC06022
Type TransgeneCartagena
Species Mus musculus
Strain name ICR-Tg(Olig2-luc2/Olig2)1Imayo
Former Common name Luc2-Olig2 fusion BA ; Olig2-luc2/Olig2 Tg#1
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2011. The transgene was injected into the pronuclei of ICR fertilized eggs.
Strain description Olig2 BAC transgenic mice, in which luc2-Olug2 fusion protein is expressed.
Colony maintenance Carrier x Noncarrier [or Crossing to Slc:ICR]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol HA
Symbol name human influenza virus HA (hemagglutinin) tag sequence
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol luc2
Symbol name Photinus pyralis Luciferase
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Science DOI: 10.1126/science.1242366
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
Additional information
1 No Data
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06590, C57BL/6-Tg(Gt(ROSA)26Sor-C1V1/EYFP)1.2Imayo

RBRC No. RBRC06590
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(Gt(ROSA)26Sor-C1V1/EYFP)1.2Imayo
Former Common name R26 BAC::pCAG-FRT-C1V1-EYFP, B6-ROSA26/C1V1-YFP/Frt Tg
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Itaru Imayoshi, Institute for Virus Research, Kyoto University in 2012. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs.
Strain description C1V1-EYFP(E122T/E162T) is expressed after both Cre- and FLP-recombination. The transgene construct is knocked into Rosa26 BAC. This modified BAC was used for Tg mouse production. To achieve high-level transgene expression, C1V1-EYFP is expressed under CAG promoter and WPRE sequence is inserted into 3'UTR region. R26 BAC::pCAG-LF-C1V1-EYFP line (RBRC06588), R26 BAC::pCAG-LoxP-C1V1-EYFP line (RBRC06589) (FRT-Stop-FRT cassette was removed by crossing pCAG-FLPe strain), R26 BAC::pCAG-FRT-C1V1-EYFP line (RBRC06590) (LoxP-Stop-LoxP cassette was removed by crossing TNAP-Cre strain).
Colony maintenance Carrier x Noncarrier [C57BL/6JJmsSlc]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name beta-globin poly A signal (Rabbit), SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol EYFP
Symbol name Enhanced Yellow Fluorescent Protein (Jellyfish)
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome UN
Common name No Data
Symbol description No Data
4 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome UN
Common name No Data
Symbol description No Data
5 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome UN
Common name No Data
Symbol description No Data
6 Symbol WPRE
Symbol name Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)
Chromosome UN
Common name No Data
Symbol description No Data
7 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
8 Symbol No Data
Symbol name C1V1 (Chlamydomonas reinhardtii, Volvox carteri)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
(後日指定)
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). The RECIPIENT of BIOLOGICAL RESOURCE must obtain a prior written consent on use of it from the DEVELOPER of the C1V1·ChR2(SSFO) (Dr. Karl Deisseroth, http://web.stanford.edu/group/dlab/optogenetics/request_dna.html).
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC05147, B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-mTFP1)Imayo>

RBRC No. RBRC05147
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-mTFP1)Imayo>
Former Common name R26-CAG-LoxP-mTFP1
H-2 Haplotype No Data
ES cell line TC1 [129S6/SvEvTac]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description HAtag-attached mTFP1 fluorescent protein is expressed after Cre-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, HA-mTFP1 is expressed under CAG promoter and WPRE sequence is inserted into 3'UTR region.
Health Report
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name STOP cassette (SV40pA-TKpA-SV40pA)
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol GH
Symbol name growth hormone polyadenylation signal
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol HA
Symbol name human influenza virus HA (hemagglutinin) tag sequence
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol SA
Symbol name SA (splice acceptor sequence)
Chromosome 6
Common name No Data
Symbol description No Data
7 Symbol WPRE
Symbol name Woodchuck hepatitis virus posy-transcriptional regulatory element
Chromosome 6
Common name No Data
Symbol description No Data
8 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
9 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
10 Symbol mTFP1
Symbol name monomeric Teal Fluorescent Protein 1 (Clavularia sp.)
Chromosome 6
Common name No Data
Symbol description No Data
11 Symbol polyA
Symbol name SV40 late polyA signal
Chromosome 6
Common name No Data
Symbol description No Data
12 Symbol puro
Symbol name puromycin resistance gene (Streptomyces alboniger)
Chromosome 6
Common name No Data
Symbol description No Data
13 Symbol tk
Symbol name HSV thymidine kinase polyA signal
Chromosome 6
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neurosci. Res., 73, 85-91 (2012).
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
Additional information
1 Itaru Imayoshi HP
2 Genotyping protocol <PCR>
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC05163, B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-GCaMP3)Imayo>

RBRC No. RBRC05163
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-GCaMP3)Imayo>
Former Common name R26-CAG-LoxP-GCaMP3
H-2 Haplotype No Data
ES cell line TC1 [129S6/SvEvTac]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description GCaMP3 is expressed after Cre-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, GCaMP3 is expressed under CAG promoter and WPRE sequence is inserted into 3'UTR region.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name HSV thymidine kinase polyA signal
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol No Data
Symbol name SV40 late polyA signal
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol GH
Symbol name growth hormone polyadenylation signal
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol SA
Symbol name splice acceptor sequence
Chromosome 6
Common name No Data
Symbol description No Data
7 Symbol WPRE
Symbol name Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE) pA
Chromosome 6
Common name No Data
Symbol description No Data
8 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
9 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
10 Symbol puro
Symbol name puromycin resistance gene (Streptomyces alboniger)
Chromosome 6
Common name No Data
Symbol description No Data
Promoter CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)
11 Symbol GCaMP3
Symbol name RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA
Chromosome 6
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Cell Rep., 9(5):1896-1907 (2014).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR.
Additional information
1 Dr. Loren Looger, MTA
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Itaru Imayoshi HP
2 Genotyping protocol <PCR>
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months
BRC mice in Publications
Title Journal
(PMID)
Author
Transient elevation of cytoplasmic calcium ion concentration at a single cell level precedes morphological changes of epidermal keratinocytes during cornification. Sci. Rep.8(1): 6610 (2018).(29700333)
Murata T, Honda T, Egawa G, Yamamoto Y, Ichijo R, Toyoshima F, Dainichi T, Kabashima K.

RBRC06002, ICR.Cg-Gt(ROSA)26Sor<tm1(Hes1,EGFP)Imayo>

RBRC No. RBRC06002
Type Gene Trap
Species Mus musculus
Strain name ICR.Cg-Gt(ROSA)26Sor<tm1(Hes1,EGFP)Imayo>
Former Common name Rosa26R-Hes1-IRES-EGFP ; ROSA26/Hes1GFP
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2008. TT2 ES cells were used. The mutant mice were crossed to ICR.
Strain description Hes1-IRES-EGFP Rosa26 knock-in mice. Expression of Hes1 and EGFP can be induced after Cre-mediated recombination.
Colony maintenance Homozygote x Homozygote [or Crossing to Slc:ICR]
Health Report
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name SV40 late polyA signal
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol GH
Symbol name Growth hormone polyA (Bovine)
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol Hes1
Symbol name hairy and enhancer of split 1 (Drosophila)
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol Ires
Symbol name internal ribosomal entry site (EMCV)
Chromosome 6
Common name No Data
Symbol description No Data
7 Symbol SA
Symbol name adenovirus splice acceptor sequence (SA)
Chromosome 6
Common name No Data
Symbol description No Data
8 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
9 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 6
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Science DOI: 10.1126/science.1242366
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06023, ICR.Cg-Tg(Sox2-luc2/Sox2)1Imayo

RBRC No. RBRC06023
Type Transgene CongenicCartagena
Species Mus musculus
Strain name ICR.Cg-Tg(Sox2-luc2/Sox2)1Imayo
Former Common name Luc2-Sox2 fusion BAC ; Sox2-luc2/Sox2 Tg#1
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2011. The transgene was injected into the pronuclei of ICR fertilized eggs.
Strain description Sox2 BAC transgenic mice, in which luc2-Sox2 fusion protein is expressed.
Colony maintenance Carrier x Noncarrier [or Crossing to Slc:ICR]
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol HA
Symbol name human influenza virus HA (hemagglutinin) tag sequence
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
4 Symbol luc2
Symbol name Photinus pyralis Luciferase
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Science DOI: 10.1126/science.1242366
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
Additional information
1 No Data
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05148, B6;129S6-Gt(ROSA)26Sor<tm1.2(CAG-mTFP1)Imayo>

RBRC No. RBRC05148
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129S6-Gt(ROSA)26Sor<tm1.2(CAG-mTFP1)Imayo>
Former Common name R26-CAG-FRT-mTFP1
H-2 Haplotype No Data
ES cell line TC1 [129S6/SvEvTac]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description HAtag-attached mTFP1 fluorescent protein is expressed after FLP-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, HA-mTFP1 is expressed under CAG promoter and WPRE sequence is inserted into 3'UTR region.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name STOP cassette (SV40pA-TKpA-SV40pA)
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol GH
Symbol name growth hormone polyadenylation signal
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol HA
Symbol name human influenza virus HA (hemagglutinin) tag sequence
Chromosome 6
Common name No Data
Symbol description No Data
7 Symbol SA
Symbol name SA (splice acceptor sequence)
Chromosome 6
Common name No Data
Symbol description No Data
8 Symbol WPRE
Symbol name Woodchuck hepatitis virus posy-transcriptional regulatory element
Chromosome 6
Common name No Data
Symbol description No Data
9 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
10 Symbol mTFP1
Symbol name monomeric Teal Fluorescent Protein 1 (Clavularia sp.)
Chromosome 6
Common name No Data
Symbol description No Data
11 Symbol polyA
Symbol name SV40 late polyA signal
Chromosome 6
Common name No Data
Symbol description No Data
12 Symbol puro
Symbol name puromycin resistance gene (Streptomyces alboniger)
Chromosome 6
Common name No Data
Symbol description No Data
13 Symbol tk
Symbol name HSV thymidine kinase polyA signal
Chromosome 6
Common name No Data
Symbol description No Data
References

Research applications FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neurosci. Res., 73, 85-91 (2012).
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
Additional information
1 Itaru Imayoshi HP
2 Genotyping protocol <PCR>
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months