RBRC05150, B6;129S6-Gt(ROSA)26Sor<tm1(CAG-eNpHR2/EYFP)Imayo>

RBRC No. RBRC05150
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129S6-Gt(ROSA)26Sor<tm1(CAG-eNpHR2/EYFP)Imayo>
Former Common name R26-CAG-LF-eNpHR2-EYFP
H-2 Haplotype No Data
ES cell line TC1 [129S6/SvEvTac]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description eNpHR2-EYFP is expressed after both Cre- and FLP-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, eNpHR2-EYFP is expressed under CAG promoter and WPRE sequence is inserted into 3'UTR region.
Health Report
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol EYFP
Symbol name Enhanced Yellow Fluorescent Protein (Jellyfish)
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol WPRE
Symbol name Woodchuck hepatitis virus posy-transcriptional regulatory element
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol eNpHR2
Symbol name Natronomonas enhanced halorhodopsin 2
Chromosome 6
Common name No Data
Symbol description No Data
7 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
8 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
9 Symbol poly A
Symbol name SV40 poly A
Chromosome 6
Common name No Data
Symbol description No Data
10 Symbol puro
Symbol name puromycin resistance gene (Streptomyces alboniger)
Chromosome 6
Common name No Data
Symbol description No Data
11 Symbol tpA
Symbol name SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal
Chromosome 6
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neurosci. Res., 75(1):53-58 (2013).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR.
Additional information
1 Dr. Karl Deisseroth, MTA
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Itaru Imayoshi HP
2 Genotyping protocol <PCR>
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05166, B6;B6CB-Slc17a7<tm1.1(IRES-tTAad)Imayo>

RBRC No. RBRC05166
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;B6CB-Slc17a7<tm1.1(IRES-tTAad)Imayo>
Former Common name VGLUT1-LoxP-Stop-LoxP-IRES2-tTA-Ad
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description tTA-Ad is expressed after Cre-recombination in VGLUT1-expressing cells. LoxP-Stop-LoxP-IRES2-tTA-Ad sequence is knocked into 3'UTR region of VGLUT1 gene.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 7
Common name No Data
Symbol description No Data
3 Symbol Ires
Symbol name internal ribosomal entry site (EMCV)
Chromosome 7
Common name No Data
Symbol description No Data
4 Symbol Slc17a7
Symbol name solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
Chromosome 7
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
6 Symbol tTAad
Symbol name tTA-Advanced (synthetic)
Chromosome 7
Common name No Data
Symbol description No Data
References

Research applications FLP/frt system,
Tet system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
The Journal of Neuroscience, 34, 5788-5799 (2014).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
Additional information
1 Itaru Imayoshi HP
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06006, ICR-Tg(Hes5-ECFP/PSLD)2Imayo

RBRC No. RBRC06006
Type TransgeneCartagena
Species Mus musculus
Strain name ICR-Tg(Hes5-ECFP/PSLD)2Imayo
Former Common name pHes5-f-ECFP-PSLD Line2 ; Hes5-CFP/PSLD Tg#2
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2010. The transgene was injected into the pronuclei of ICR fertilized eggs.
Strain description Hes5-ECFP/PSLD transgenic mice. Cell-cycle phase marker, ECFP-PSLD is expressed under the control of Hes5 promoter.
Colony maintenance Carrier x Noncarrier [or Crossing to Slc:ICR]
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name No Data
Chromosome No Data
Common name No Data
Symbol description No Data
References No Data

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
後日指定。(will be announced).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
Additional information
1 No Data
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Preparing for distribution
Preparing for distribution
(Please note that it may take more than 6 months to make arrangements to distribute.)

Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

RBRC06047, B6.Cg-Hes1<tm1Imayo>

RBRC No. RBRC06047
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name B6.Cg-Hes1<tm1Imayo>
Former Common name B6-Hes1-floxed ; B6-Hes1 floxed
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2007. TT2 ES cells were used. The mutant mice were crossed to C57BL/6 for 6 times.
Strain description Hes1 gene floxed mice. LoxP sequences were knocked into the first intron and downstream of 3’UTR of Hes1 gene for conditional knock-out study. PGK-Neo sequence was removed.
Colony maintenance Homozygote x Homozygote [or Crossing to C57BL/6JJcl]
Health Report
Gene Details
Promoter No Data
1 Symbol Hes1
Symbol name hairy and enhancer of split 1 (Drosophila)
Chromosome 16
Common name No Data
Symbol description No Data
2 Symbol loxP
Symbol name phage P1 loxP
Chromosome 16
Common name No Data
Symbol description No Data
References Development. 2008 Aug;135(15):2531-41. Epub 2008 Jun 25.

Hes genes and neurogenin regulate non-neural versus neural fate specification in the dorsal telencephalic midline.
Research applications Cre/loxP system,
Developmental Biology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Development. 2008 135(15):2531-41.
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC05151, B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-eNpHR2/EYFP)Imayo>

RBRC No. RBRC05151
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129S6-Gt(ROSA)26Sor<tm1.1(CAG-eNpHR2/EYFP)Imayo>
Former Common name R26-CAG-LoxP-eNpHR2-EYFP
H-2 Haplotype No Data
ES cell line TC1 [129S6/SvEvTac]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description eNpHR2-EYFP is expressed after Cre-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, eNpHR2-EYFP is expressed under CAG promoter and WPRE sequence is inserted into 3'UTR region.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol EYFP
Symbol name Enhanced Yellow Fluorescent Protein (Jellyfish)
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol WPRE
Symbol name Woodchuck hepatitis virus posy-transcriptional regulatory element
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol eNpHR2
Symbol name Natronomonas enhanced halorhodopsin 2
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
7 Symbol polyA
Symbol name SV40 polyA
Chromosome 6
Common name No Data
Symbol description No Data
8 Symbol puro
Symbol name puromycin resistance gene (Streptomyces alboniger)
Chromosome 6
Common name No Data
Symbol description No Data
9 Symbol tpA
Symbol name SV40 late polyA signal, HSV thymidine kinase polyA signal, SV40 early polyA signal
Chromosome 6
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neurosci. Res., 75(1):53-58 (2013).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR.
Additional information
1 Dr. Karl Deisseroth, MTA
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Itaru Imayoshi HP
2 Genotyping protocol <PCR>
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05167, B6;B6CB-Slc17a7<tm1.2(IRES-tTAad)Imayo>

RBRC No. RBRC05167
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;B6CB-Slc17a7<tm1.2(IRES-tTAad)Imayo>
Former Common name VGLUT1-IRES2-tTA-Ad
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description tTA-Ad is expressed in VGLUT1-expressing cells. IRES2-tTA-Ad sequence is knocked into 3'UTR region of VGLUT1 gene.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome 7
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 7
Common name No Data
Symbol description No Data
3 Symbol Ires
Symbol name internal ribosomal entry site (EMCV)
Chromosome 7
Common name No Data
Symbol description No Data
4 Symbol Slc17a7
Symbol name solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
Chromosome 7
Common name No Data
Symbol description No Data
5 Symbol loxP
Symbol name phage P1 loxP
Chromosome 7
Common name No Data
Symbol description No Data
6 Symbol tTAad
Symbol name tTA-Advanced (synthetic)
Chromosome 7
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system,
Tet system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
The Journal of Neuroscience, 34, 5788-5799 (2014).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
Additional information
1 Itaru Imayoshi HP
2 Genotyping protocol <PCR>
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06008, ICR.Cg-Hes5<tm1(venus)Imayo>

RBRC No. RBRC06008
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name ICR.Cg-Hes5<tm1(venus)Imayo>
Former Common name Venus-Hes5 fusion Knock-in ; Hes5/venus KI
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2010. TT2 ES cells were used. The mutant mice were crossed to ICR.
Strain description Venus/Hes5 knock-in mice. Venus-Hes5 fusion protein is expressed from Hes5 locus.
Colony maintenance Heterozygote x Wild-type [or Crossing to Slc:ICR]
Health Report
Gene Details
Promoter No Data
1 Symbol FLAG
Symbol name FLAG tag (synthetic)
Chromosome 4
Common name No Data
Symbol description No Data
2 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 4
Common name No Data
Symbol description No Data
3 Symbol Hes5
Symbol name hairy and enhancer of split 5 (Drosophila)
Chromosome 4
Common name No Data
Symbol description No Data
4 Symbol Venus
Symbol name Green Fluorescent Protein, F46L (Jellyfish)
Chromosome 4
Common name No Data
Symbol description No Data
References

Research applications FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Science DOI: 10.1126/science.1242366
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
Additional information
1 No Data
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06493, ICR.Cg-Hes1<tm1(venus)Imayo>

RBRC No. RBRC06493
Type Targeted MutationCartagena
Species Mus musculus
Strain name ICR.Cg-Hes1<tm1(venus)Imayo>
Former Common name Venus-Hes1 fusion KI (Neo), Hes1/venus KI
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2008. TT2 ES cells were used. The mutant mice were crossed to ICR.
Strain description Venus/Hes1 knock-in mice. Venus-Hes1 fusion protein is expressed from Hes1 locus. LoxP sequences were knocked into the first intron and downstream of 3’UTR of Hes1 gene for conditional knock-out study. delta Neo type (RBRC06010), fNeo type (RBRC06493).
Colony maintenance Heterozygote x Wild-type [or Crossing to Slc:ICR]
Health Report
Gene Details
Promoter No Data
1 Symbol Hes1
Symbol name hairy and enhancer of split 1 (Drosophila)
Chromosome 16
Common name No Data
Symbol description No Data
2 Symbol Venus
Symbol name Green Fluorescent Protein, F46L (Jellyfish)
Chromosome 16
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome 16
Common name No Data
Symbol description No Data
Promoter mouse phosphoglycerate kinase promoter (PGK promoter)
4 Symbol neo
Symbol name neomycin resistance gene (E. coli)
Chromosome 16
Common name No Data
Symbol description No Data
References No Data

Research applications Cre/loxP system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Science DOI: 10.1126/science.1242366
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
Additional information
1 No Data
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05152, B6;129S6-Gt(ROSA)26Sor<tm1.2(CAG-eNpHR2/EYFP)Imayo>

RBRC No. RBRC05152
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129S6-Gt(ROSA)26Sor<tm1.2(CAG-eNpHR2/EYFP)Imayo>
Former Common name R26-CAG-FRT-eNpHR2-EYFP
H-2 Haplotype No Data
ES cell line TC1 [129S6/SvEvTac]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description eNpHR2-EYFP is expressed after FLP-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, eNpHR2-EYFP is expressed under CAG promoter and WPRE sequence is inserted into 3'UTR region.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol EYFP
Symbol name Enhanced Yellow Fluorescent Protein (Jellyfish)
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol WPRE
Symbol name Woodchuck hepatitis virus posy-transcriptional regulatory element
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol eNpHR2
Symbol name Natronomonas enhanced halorhodopsin 2
Chromosome 6
Common name No Data
Symbol description No Data
7 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
8 Symbol poly A
Symbol name poly A
Chromosome 6
Common name No Data
Symbol description No Data
9 Symbol puro
Symbol name puromycin resistance gene (Streptomyces alboniger)
Chromosome 6
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neurosci. Res., 75(1):53-58 (2013).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR.
Additional information
1 Dr. Karl Deisseroth, MTA
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Itaru Imayoshi HP
2 Genotyping protocol <PCR>
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05168, C57BL/6-Tg(Th-FLPo)4Imayo

RBRC No. RBRC05168
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(Th-FLPo)4Imayo
Former Common name pTH-FLPo line4
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description FLPo recombinase is expressed under the TH promoter.
Health Report No Data
Gene Details
Promoter rat tyrosine hydroxylase (TH) promoter
1 Symbol FLPo
Symbol name yeast flippase recombinase, a codon improved version of the flpe site specific recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications FLP/frt system,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neursci. Res., 73, 85-91 (2012).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
Additional information
1 Itaru Imayoshi HP
2 Genotyping protocol <PCR>
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months