RBRC05152, B6;129S6-Gt(ROSA)26Sor<tm1.2(CAG-eNpHR2/EYFP)Imayo>

RBRC No. RBRC05152
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129S6-Gt(ROSA)26Sor<tm1.2(CAG-eNpHR2/EYFP)Imayo>
Former Common name R26-CAG-FRT-eNpHR2-EYFP
H-2 Haplotype No Data
ES cell line TC1 [129S6/SvEvTac]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description eNpHR2-EYFP is expressed after FLP-recombination. The transgene construct is knocked into Rosa26-locus. To achieve high-level transgene expression, eNpHR2-EYFP is expressed under CAG promoter and WPRE sequence is inserted into 3'UTR region.
Health Report No Data
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol EYFP
Symbol name Enhanced Yellow Fluorescent Protein (Jellyfish)
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol WPRE
Symbol name Woodchuck hepatitis virus posy-transcriptional regulatory element
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol eNpHR2
Symbol name Natronomonas enhanced halorhodopsin 2
Chromosome 6
Common name No Data
Symbol description No Data
7 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
8 Symbol poly A
Symbol name poly A
Chromosome 6
Common name No Data
Symbol description No Data
9 Symbol puro
Symbol name puromycin resistance gene (Streptomyces alboniger)
Chromosome 6
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neurosci. Res., 75(1):53-58 (2013).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary). The RECIPIENT of the BIOLOGICAL RESOURCE is requested to make a Material Transfer Agreement specified by the DEPOSITOR.
Additional information
1 Dr. Karl Deisseroth, MTA
GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Itaru Imayoshi HP
2 Genotyping protocol <PCR>
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05168, C57BL/6-Tg(Th-FLPo)4Imayo

RBRC No. RBRC05168
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(Th-FLPo)4Imayo
Former Common name pTH-FLPo line4
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description FLPo recombinase is expressed under the TH promoter.
Health Report No Data
Gene Details
Promoter rat tyrosine hydroxylase (TH) promoter
1 Symbol FLPo
Symbol name yeast flippase recombinase, a codon improved version of the flpe site specific recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications FLP/frt system,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neursci. Res., 73, 85-91 (2012).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
Additional information
1 Itaru Imayoshi HP
2 Genotyping protocol <PCR>
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06009, ICR.Cg-Hes5<tm1(Eluc)Imayo>

RBRC No. RBRC06009
Type Targeted Mutation CongenicCartagena
Species Mus musculus
Strain name ICR.Cg-Hes5<tm1(Eluc)Imayo>
Former Common name Eluc-Hes5 fusion Knock-in ; Hes5/Eluc KI
H-2 Haplotype No Data
ES cell line TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2010. TT2 ES cells were used. The mutant mice were crossed to ICR.
Strain description Eluc/Hes5 knock-in mice. Eluc-Hes5 fusion protein is expressed from Hes5 locus.
Colony maintenance Heterozygote x Wild-type [or Crossing to Slc:ICR]
Health Report
Gene Details
Promoter No Data
1 Symbol Hes5
Symbol name hairy and enhancer of split 5 (Drosophila)
Chromosome 4
Common name No Data
Symbol description No Data
References

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Science DOI: 10.1126/science.1242366
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06580, C57BL/6-Tg(Dlx5/6-tTAad)1Imayo

RBRC No. RBRC06580
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(Dlx5/6-tTAad)1Imayo
Former Common name Dlx5/6-tTA-Ad Line1, B6-Dlx-tTAad Tg#1
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Itaru Imayoshi, Institute for Virus Research, Kyoto University in 2010. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs.
Strain description tTA-Ad is expressed under the Dlx5/6 enhancer. Line 1 (RBRC06580), Line 2 (RBRC06581), Line 3 (RBRC06582).
Colony maintenance Carrier x Noncarrier [C57BL/6JJmsSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name immunoglobulin gene heavy chain variable region (human)
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol HA
Symbol name human influenza virus HA (hemagglutinin) tag sequence
Chromosome UN
Common name No Data
Symbol description No Data
4 Symbol HBB2
Symbol name human beta-globin
Chromosome UN
Common name No Data
Symbol description No Data
5 Symbol WPRE
Symbol name Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Dlx5/6 enhancer
6 Symbol tTAad
Symbol name tTA-Advanced (synthetic)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Neurobiology Research,
Tet system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
(後日指定)
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

Live
Live mouse :

RBRC05153, B6;129S6-Gt(ROSA)26Sor<tm1(CAG-EGFP/TeNT)Imayo>

RBRC No. RBRC05153
Type Targeted MutationCartagena
Species Mus musculus
Strain name B6;129S6-Gt(ROSA)26Sor<tm1(CAG-EGFP/TeNT)Imayo>
Former Common name R26-CAG-LF-EGFP-TeNT
H-2 Haplotype No Data
ES cell line TC1 [129S6/SvEvTac]
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain description EGFP-TeNTpest is expressed after both Cre- and FLP-recombination. The transgene construct is knocked into Rosa26-locus. EGFP-TeNTpest is expressed under CAG promoter.
Health Report
Gene Details
Promoter No Data
1 Symbol Gt(ROSA)26Sor
Symbol name gene trap ROSA 26, Philippe Soriano
Chromosome 6
Common name No Data
Symbol description No Data
2 Symbol EGFP
Symbol name Enhanced Green Fluorescent Protein, F64L and S65T (Jellyfish)
Chromosome 6
Common name No Data
Symbol description No Data
3 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome 6
Common name No Data
Symbol description No Data
4 Symbol Odc1
Symbol name mouse ornithine decarboxylase (PEST sequence) cDNA
Chromosome 6
Common name No Data
Symbol description No Data
5 Symbol TeNT
Symbol name tetanus neurotoxin light chain (Clostridium tetani)
Chromosome 6
Common name No Data
Symbol description No Data
6 Symbol loxP
Symbol name phage P1 loxP
Chromosome 6
Common name No Data
Symbol description No Data
7 Symbol poly A
Symbol name poly A
Chromosome 6
Common name No Data
Symbol description No Data
8 Symbol puro
Symbol name puromycin resistance gene (Streptomyces alboniger)
Chromosome 6
Common name No Data
Symbol description No Data
References

Research applications Cre/loxP system,
FLP/frt system,
Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
The Journal of Neuroscience, 34, 5788-5799 (2014).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
Itaru Imayoshi HP
2 Genotyping protocol <PCR>
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC05169, C57BL/6-Tg(Th-FLPo)5Imayo

RBRC No. RBRC05169
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(Th-FLPo)5Imayo
Former Common name pTH-FLPo line5
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain description FLPo recombinase is expressed under the TH promoter.
Health Report No Data
Gene Details
Promoter rat tyrosine hydroxylase (TH) promoter
1 Symbol FLPo
Symbol name yeast flippase recombinase, a codon improved version of the flpe site specific recombinase
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications FLP/frt system,
Neurobiology Research
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Neursci. Res., 73, 85-91 (2012).
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
Additional information
1 Itaru Imayoshi HP
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06013, ICR-Tg(Hes1-luc2/Hes1)1Imayo

RBRC No. RBRC06013
Type TransgeneCartagena
Species Mus musculus
Strain name ICR-Tg(Hes1-luc2/Hes1)1Imayo
Former Common name Luc2-Hes1 fusion BAC ; Hes1-luc3/Hes1 Tg#1
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Itaru Imayoshi and Ryoichiro Kageyama, Institute for Virus Research, Kyoto University in 2011. The transgene was injected into the pronuclei of ICR fertilized eggs.
Strain description Hes1 BAC transgenic mice, in which Luc2-Hes1 fusion protein is expressed.
Colony maintenance Carrier x Noncarrier [or Crossing to Slc:ICR]
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol HA
Symbol name human influenza virus HA (hemagglutinin) tag sequence
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol loxP
Symbol name phage P1 loxP
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Hes1 promoter
4 Symbol luc2
Symbol name Photinus pyralis Luciferase
Chromosome UN
Common name No Data
Symbol description No Data
References

Research applications No Data
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Science DOI: 10.1126/science.1242366
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp) and Ryoichiro Kageyama (E-mail: rkageyam@virus.kyoto-u.ac.jp ). (No approval form (Form D) is necessary).
Additional information
1 No Data
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC06581, C57BL/6-Tg(Dlx5/6-tTAad)2Imayo

RBRC No. RBRC06581
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(Dlx5/6-tTAad)2Imayo
Former Common name Dlx5/6-tTA-Ad Line2, B6-Dlx-tTAad Tg#2
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Itaru Imayoshi, Institute for Virus Research, Kyoto University in 2010. The transgene was injected into the pronuclei of C57BL/6 fertilized eggs.
Strain description tTA-Ad is expressed under the Dlx5/6 enhancer. Line 1 (RBRC06580), Line 2 (RBRC06581), Line 3 (RBRC06582).
Colony maintenance Carrier x Noncarrier [C57BL/6JJmsSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name SV40 PolyA signal
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol No Data
Symbol name immunoglobulin gene heavy chain variable region (human)
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol HA
Symbol name human influenza virus HA (hemagglutinin) tag sequence
Chromosome UN
Common name No Data
Symbol description No Data
4 Symbol HBB2
Symbol name human beta-globin
Chromosome UN
Common name No Data
Symbol description No Data
5 Symbol WPRE
Symbol name Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Dlx5/6 enhancer
6 Symbol tTAad
Symbol name tTA-Advanced (synthetic)
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Neurobiology Research,
Tet system
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
(後日指定)
In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. The RECIPIENT of BIOLOGICAL resource shall obtain a prior written consent on use of it from the DEPOSITOR Dr. Itaru Imayoshi (E-mail: iimayosh@virus.kyoto-u.ac.jp). (No approval form (Form D) is necessary).
Additional information
1 No Data
2 Genotyping protocol <PCR>
Depositor Imayoshi, Itaru (Institute for Virus Research, Kyoto University) Imayoshi, Itaru
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months