RBRC06258, C57BL/6-Tg(Cacng5-EGFP)500Bsi

RBRC No. RBRC06258
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(Cacng5-EGFP)500Bsi
Former Common name Cacng5 promoter-GFP, B6-Cacng5-GFP Tg#500
H-2 Haplotype No Data
Background strain No Data
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Yoshiaki Shinohara and Hajime Hirose, RIKEN Brain Science Institute. The BAC transgenic construct containing the mouse Cacng5 gene with IRES-GFP (GENSAT1-BX1300) was used. C57BL/6 background.
Strain description hese transgenic mice express IRES-GFP under the control of the mouse Cacng5 promoter.
Colony maintenance Carrier x Noncarrier [C57BL/6JJmsSlc]
Health Report
Gene Details
Promoter No Data
1 Symbol No Data
Symbol name No Data
Chromosome No Data
Common name No Data
Symbol description No Data
References

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.
Eur. J. Neurosci., 35, 702-710 (2012 ).
The use of the BIOLOGICAL RESOURCE is strictly limited to academic, non-profit research institutes for academic non-profit research purposes. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, a mention of the GENSAT Project (GENSAT1-BX1300) as the source of the original BAC transgenic construct are requested.
Additional information
1 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
2 Genotyping protocol <PCR>
Depositor Hirase, Hajime (RIKEN) Hirase, Hajime
Strain Status /
Availability
(Expected delivery)


Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months

RBRC09650, C57BL/6-Tg(Slc1a2-G-CaMP7)Bsi

RBRC No. RBRC09650
Type TransgeneCartagena
Species Mus musculus
Strain name C57BL/6-Tg(Slc1a2-G-CaMP7)Bsi
Former Common name GLT1-G-CaMP7, G7NG817
H-2 Haplotype No Data
Background strain C57BL/6JJcl
Appearance
1 Appearance No Data
Genotype No Data
Strain development Developed by Hajime Hirase, Brain Science Institute, RIKEN in 2016. A BAC DNA construct was injected to fertilized eggs derived from C57BL/6J. Mice were maintained in this genetic background. Homozygous mice are viable and fertile.
Strain description G-CaMP7 transgenic mice under the control of Slc1a2 (GLT-1) promoter. Astrocytes and a subset of cortical neurons express G-CaMP7.
Health Report
Gene Details
Promoter No Data
1 Symbol Frt
Symbol name yeast FRT (flippase recombination target) site
Chromosome UN
Common name No Data
Symbol description No Data
2 Symbol HBB2
Symbol name rabbit beta-globin polyA
Chromosome UN
Common name No Data
Symbol description No Data
3 Symbol WPRE
Symbol name Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)
Chromosome UN
Common name No Data
Symbol description No Data
Promoter mouse Slc1a2 promoter
4 Symbol GCaMP7
Symbol name RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA
Chromosome UN
Common name No Data
Symbol description No Data
References No Data

Research applications Fluorescent Proteins/lacZ System
Specific Term and Conditions The following terms and conditions will be requested by the DEPOSITOR.
In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the above literature designated by the DEPOSITOR is recommended. Nat Commun. (2016) 7:11100. DOI: 10.1038/ncomms11100
Additional information
1 Mouse of the Month Aug 2017
2 GFP Transfer License (Japanese / English)
Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC’s MTA. Please also read Schedule B.
3 Genotyping protocol <PCR>
Depositor Hirase, Hajime (RIKEN) Hirase, Hajime
Strain Status /
Availability
(Expected delivery)


Frozen
Cryopreserved embryos : Within 1 month
Recovered litters from cryopreserved embryos : 2-4 months

Sperm
Cryopreserved sperm : Within 1 month
Recovered litters from cryopreserved sperm : 2-4 months